methods and protocols methods in molecular biology vol 700

... establish clean point mutations and in-frame deletion mutations that have been instrumental in identifying and understanding the molecular interactions between components of the Yersinia type III ... dendritic cells This interaction causes an increase in pro-infl ammatory cytokines and chemokines The increase in infl ammation eventually leads to edema, erythema, abscess formation, and mucosal hemorrhages ... synthesis and secretion translocators have been studied in some detail Utilizing in-frame deletion mutagenesis uncovered two LcrH-interacting domains in YopD—the fi rst being a large N-terminal domain

Ngày tải lên: 25/12/2016, 15:05

... Nikiforov (ed.), Oncogene-Induced Senescence: Methods and Protocols, Methods in Molecular Biology, vol 1534, DOI 10.1007/978-1-4939-6670-7_1, © Springer Science+Business Media New York 2017 ... as insulin-like growth factor-binding proteins (IGFBPs) [ 23 ] and interleukin-6 (IL-6) (reviewed in ref 24 ) These observations indicate that senescence is not just dictated by events happening ... gelatin and let coverslips dry before cell plating 7 Cells should always be plated in duplicate for experimental replicates 8 SA-βgal staining should be performed in the evening Staining

Ngày tải lên: 25/12/2016, 15:07

... and 2 (HTLV-1 and HTLV-2) infections in HTLV seroindeterminate individuals from Sao Paulo, Brazil J Clin Virol 44(3):185–189 80 Garin B, Gosselin S, de The G, Gessain A (1994) HTLV-I/II infection ... HTLV indeterminate pattern provisionally called “New HTLV indeterminate” indeterminate pattern (T–Z) HTLV-1 Infection Epidemiology and Diagnosis Trang 25discriminate between HTLV-1 and HTLV-2 infections ... protein (GD21) of HTLV-1 and HTLV-2 as well as specific synthetic peptides originating from HTLV-1 (including MTA-I or rgp46-I) and HTLV-2 (including K55 or recognize both HTLV-1 and HTLV-2 infection

Ngày tải lên: 13/05/2017, 21:19

... 30Ingrid C Gelissen and Andrew J Brown (eds.), Cholesterol Homeostasis: Methods and Protocols, Methods in Molecular Biology, vol 1583, DOI 10.1007/978-1-4939-6875-6_3, © Springer Science+Business ... Homeostasis: Methods and Protocols, Methods in Molecular Biology, vol 1583, DOI 10.1007/978-1-4939-6875-6_1, © Springer Science+Business Media LLC 2017 Chapter 1 An Overview of Cholesterol Homeostasis Ingrid ... Cholesterol-Binding Domains in Membrane Proteins Coralie Di Scala and Jacques Fantini Abstract In eukaryotic cells, cholesterol is an important regulator of a broad range of membrane proteins, including

Ngày tải lên: 13/05/2017, 21:19

... Snick J (2006) Allergen- induced interleukin-9 production in vitro: correlation with atopy in human adults and comparison with interleukin-5 and interleu-kin- 13 Clin Exp Allergy 36(2):174–182 ... Th9 Cells: Methods and Protocols, Methods in Molecular Biology, vol 1585, DOI 10.1007/978-1-4939-6877-0_2, © Springer Science+Business Media LLC 2017 Chapter 2 IL-9: Function, Sources, and Detection ... Study of Inflammatory Diseases in Research and Clinical Settings 247 Patricia Keating and James X Hartmann Index 257 Contents Trang 8Silvia C.P almeida • Faculdade de Medicina, Instituto

Ngày tải lên: 13/05/2017, 21:20

... possession an ever-expanding arsenal that continues to aid us in dissecting the function of telomerase and telomere-binding proteins, probing the changes in telomeres, and elucidating the consequences ... transfer and minimize bubbles 9 The membrane may be further incubated in NaOH solution for 5–10 min to denature any remaining DNA, neutralized again, and rinsed in 2× SSC before cross-linking 10 ... insights into the mechanism of action, adap-Nature reviews Microbiology 14:67–76 Trang 24Zhou Songyang (ed.), Telomeres and Telomerase: Methods and Protocols, Methods in Molecular Biology, vol

Ngày tải lên: 13/05/2017, 21:21

... of interest 2 Hongseok Ha et al. Trang 13For studies involving multiple samples, Illumina provides6 bp index sequences for pooling multiple samples in one ing library We tested 48 indexes and ... blocking during runs 16 If tips are to be used again within 24 h they can remain in thetip holder and be stored in the fridge, inside the black box.Ensure that there are some volumes of NDTs remaining ... solution obtained homoge-22 Gwen Hoad and Kristina Harrison Trang 326 Prepare Binding Buffer/Streptavidin Sepharose bead mix.38 μL Binding buffer and 2 μL beads are required per well.Prepare volume

Ngày tải lên: 13/05/2017, 21:21

... quantitative information on immune cell migration and interactions including the velocity, meandering index, turning angles, duration and timing of interactions, and physiological outcomes of these interactions ... Trafficking: Methods and Protocols, Methods in Molecular Biology, vol 1591, DOI 10.1007/978-1-4939-6931-9_1, © Springer Science+Business Media LLC 2017 Key words Multiphoton, Modeling, 3D imaging, ... University of Birmingham Birmingham, UK Trang 5Welcome to the second edition of Methods and Protocols for assessing T cell Trafficking, in the Methods in Molecular Biology series The trafficking of T

Ngày tải lên: 13/05/2017, 21:21

... Contributors Trang 11Jing Lin and Marcos Alcocer (eds.), Food Allergens: Methods and Protocols, Methods in Molecular Biology, vol 1592, DOI 10.1007/978-1-4939-6925-8_1, © Springer Science+Business Media ... immunodetection and providing certain discriminating character-istics such as isoelectric points or molecular mass Using 2DGE, proteins are fractionated according to their isoelectric points in the first ... with five volumes of binding buffer (5 mL) at a flow rate of 2 mL/min 4 Elute with a linear gradient from 0 to 0.5 M NaCl in binding buffer in 15 column volumes (15 mL) Fraction containing purified

Ngày tải lên: 13/05/2017, 21:22

... Activation: Methods and Protocols, Methods in Molecular Biology, vol 1605, DOI 10.1007/978-1-4939-6988-3_1, © Springer Science+Business Media LLC 2017 Key words RNA degradation, Deadenylation, RNA-binding ... Activation: Methods and Protocols, Methods in Molecular Biology, vol 1605, DOI 10.1007/978-1-4939-6988-3_2, © Springer Science+Business Media LLC 2017 Chapter 2 Link of Zygotic Genome Activation and ... of four zinc fingers and a low- complexity activation domain, both of which are required for pro-moting DNA binding and mediating transcriptional activation During ZGA, Vfl/Zld-binding sites

Ngày tải lên: 13/05/2017, 21:24

... Protein-Ligand Binding Sites and Structures 97Hui Sun Lee and Wonpil Im 9 Local Alignment of Ligand Binding Sites in Proteins for Polypharmacology and Drug Repositioning 109Michal Brylinski ... query protein structure,including global and local structure matching to known proteins Chapter8describes G-LoSA, which finds ligand binding sites similar to a query binding site within a referencedatabase ... (ed.), Protein Function Prediction: Methods and Protocols, Methods in Molecular Biology, vol 1611, DOI 10.1007/978-1-4939-7015-5_1, © Springer Science+Business Media LLC 2017 1 Trang 12Hawkins &

Ngày tải lên: 13/05/2017, 21:25

... Viruses: Methods and Protocols, Methods in Molecular Biology, vol 1602, DOI 10.1007/978-1-4939-6964-7_2, © Springer Science+Business Media LLC 2017 gen-Key words Equine arteritis virus, EAV, Equine ... contain-ing ampicillin with an individual bacterial colony and incubate at 37 °C overnight in a shaker incubator (240 rpm) Purify the plasmid DNA using QIAprep Spin Miniprep Kit according to ... Viruses: Methods and Protocols, Methods in Molecular Biology, vol 1602, DOI 10.1007/978-1-4939-6964-7_3, © Springer Science+Business Media LLC 2017 Chapter 3 Reverse Genetics for Porcine Reproductive

Ngày tải lên: 16/05/2017, 23:21

... Netherlands Trang 12Andrew F Hill (ed.), Exosomes and Microvesicles: Methods and Protocols, Methods in Molecular Biology, vol 1545, DOI 10.1007/978-1-4939-6728-5_1, © Springer Science+Business ... sensing zone of the Andrew F Hill (ed.), Exosomes and Microvesicles: Methods and Protocols, Methods in Molecular Biology, vol 1545, DOI 10.1007/978-1-4939-6728-5_2, © Springer Science+Business ... scattered light contains information, ing detection and analysis of the particles which is employed in common and new techniques based on light scattering allow-Bearing in mind the principle of energy

Ngày tải lên: 24/12/2016, 22:31

... cell cycle proteins including G1 cyclins (Cln1 and Cln2), Cdk inhibitors The Budding and Fission Yeasts 11 (Sic1, Far1, and Rum1), and replication proteins (Cdc6 and Cdc18; reviewed in ref 55) SCF ... proteins involved in the spindle checkpoint are also conserved in plants (40), including MAD2 In maize, MAD2 is abundant at kinetochores during early mitosis but is barely detectable at kinetochores ... be involved in integrating cell cycle signals and transducing these to regulate the cytokinetic vesicle fusion machinery No homologs of polo-like kinases or aurora kinases, which are all involved...

Ngày tải lên: 08/04/2014, 12:50

... computer modeling in biotechnology These include silicon bionanodevices, carbon nanotube-biomolecular systems, lipoprotein assemblies, and protein engineering of gas-binding proteins, such as ... enabling gene therapy and tissue engineering applications (2022) 1.2 Methodology for Creating and Studying Chimeric Proteins Between Fibritin and Triple-Stranded Segments of Fibrous Proteins The ... creating and studying chimeric proteins was first developed from fundamental studies aimed at structural understanding of fibrous proteins In phage T4 fibritin, a 27-amino acid (aa) domain (amino...

Ngày tải lên: 04/06/2014, 15:06

... of Plasmocin; and (3) the use of a combination therapy applying the two antibiotics minocycline (tetracycline) and tiamulin (macrolide) in alternating cycles (BM-Cyclin) (4) (see Fig and Note ... use of cell lines in science and biotechnology continues to increase, the possibility of inadvertent mixture of cell lines during the course of From: Methods in Molecular Biology, vol 290: Basic ... of cell lines using DNA fingerprinting and cytogenetic evaluation have shown a high incidence (approx 15%) of false cell lines observed among cell lines obtained directly from original investigators...

Ngày tải lên: 08/04/2014, 12:50

... Cataloging -in- Publication Data Nanobiotechnology protocols / edited by Sandra J Rosenthal and David W Wright p cm (Methods in molecular biology ; 303) Includes bibliographical references and index ... Plants: Methods and Protocols, edited by Leandro Peña, 2005 285 Cell Cycle Control and Dysregulation Protocols: Cyclins, Cyclin-Dependent Kinases, and Other Factors, edited by Antonio Giordano and ... 307.Phosphodiesterase Methods and Protocols, edited by Claire Lugnier, 2005 306 Receptor Binding Techniques: Second Edition, edited by Anthony P Davenport, 2005 305.Protein–Ligand Interactions: Methods and Protocols, ...

Ngày tải lên: 04/06/2014, 15:14

... mesoderm-inducing factors and the cloning of genes encoding them, it soon became routine to induce caps by injecting in vitro-transcribed RNA into embryos in the first few cell cycles and subsequently ... embryos, in which maternal dorsal determinants have been inactivated or removed, have been an effective tool for analyzing From: Methods in Molecular Biology, Vol 127: Molecular Methods in Developmental ... polyacrylamide gel and detected by autoradiography From: Methods in Molecular Biology, Vol 127: Molecular Methods in Developmental Biology: Xenopus and Zebrafish Edited by: M Guille © Humana Press Inc., Totowa,...

Ngày tải lên: 11/04/2014, 09:52

... C-terminal domain binds to cytoskeletal proteins such as spectrin and tubulin, while its N-terminal 89-kDa ankyrin-repeat domain binds to integral membrane proteins, such as ion channels and cell ... protein) Ankyrin-binding glycoprotein 205 (AB-GP205) Ankyrins Erythroid ankyrin (Ankl) Epithelial anlgwin(s) Brain ankyrin (Ank2) Brain ankyrin (Ank2) Epithelial ankyrin(s) Epithelial ankyrin(s) ... results indicate that iPLA.2~ contains eonsensns phosphorylation sites for calcium/calmodulin-dependent protein kinase II, protein kinase A, protein kinase C, protein kinase G, and casein kinase...

Ngày tải lên: 05/05/2014, 16:43

... ending at 517, the second coding segment starting from 646 and ending at 1735, and the third coding segment starting from 1933 and ending at 2165 The complete coding sequence is made by joining ... the rapidly expanding interdisciplinary science In short, the material is developed in the spirit of the student-centered learning which is now gaining acceptance and popularity in universities ... find this book exactly what you have been looking for Acknowledgement added in the second printing Perhaps nothing is more gratifying than preparing one’s first book for the second printing, and...

Ngày tải lên: 08/04/2014, 12:50