mechanisms for activating cell death in cancer cells pdf

... were performed with cells inpassage 4–9 The hybrid EA.hy926 cell line, generated by fusion of HUVEC with cells of the lung carcinoma cell line A549 [36], was cultured in 1% gelatine-coated cell ... in endothelial cells, we repeated these exper-iments in the endothelial hybrid cell line Ea.hy926 Similar to the finding in HUVEC, the NHE1 mRNA level was decreased by about 50% in Ea.hy926 cells ... alone Tumor cell conditioned medium exposure increases protein expression of Akt1 in HUVEC In the in vivo tumor microenvironment, cancer cells and stromal cells secrete VEGF and other cytokines and...

Ngày tải lên: 06/08/2020, 06:45

... Types of cell death 9 1.2.1 Type I cell death – apoptosis 10 1.2.2 Type II cell death – autophagic cell death 16 1.2.3 Type III cell death – necrosis 21 1.3 Programmed cell death (PCD) in protozoan ... lectin binding to glycoproteins (Welburn et al., 1996) The proto-oncogene prohibitin and a receptor for activated protein kinase C was shown to be up-regulated in concanavalin A-induced cell death ... Jing Yin and Kevin S W Tan Staurosporine-induced programmed cell death in Blastocystis In 4th International Conference on Anaerobic Protists, 12 – 16 May 2008, Taoyuan, Taiwan Binhui Wu, Jing...

Ngày tải lên: 16/10/2015, 15:35

... determine the percentage of cells within a population that are actively undergoing apoptotic cell death For the cell-death assessment, the cells were plated in 60 mm dish at a density of 2· 105cells ... examine the kinetics of the apoptotic cell death induced by ionizing radiation in human cervical cancer cells, we treated HeLa cells with 10 Gy radiation, and analyzed induction of apoptotic cell ... apoptotic cell death in response to various stimuli To investigate a potential involvement of MAPK in ionizing radiation-induced cell death, we employed specific chemical inhibitors of MAPK As shown in...

Ngày tải lên: 07/03/2014, 05:20

... AccessThe Akt-inhibitor Erufosine induces apoptotic cell death in prostate cancer cells and increases the short term effects of ionizing radiation Justine Rudner1, Carola-Ellen Ruiner1, René Handrick2,3, ... reducing the number of viable cells in the WST-1 assay, ionizing radiation did not induce sig-nificant apoptotic nuclear fragmentation in LNCaP cells (Figure 2D) In line with these findings, ... inhibitor LY294002 Moreover, the efficacy of combined treatment with Akt inhibitors and ionizing radiation in prostate cancer cells was examined Materials and methods: Prostate cancer cell lines...

Ngày tải lên: 09/08/2014, 09:20

... MB-PDT in selectively eliminating tumour cells while not affecting normal-like cells Methods Cell cultures Non-tumorigenic human MCF-10A (ATCC CRL-10317™) breast cell line was maintained in phenol ... The incorporation of MB was determined by correcting the absorbance of MB by the number of cells remaining in each well after the incubation period Intracellular singlet oxygen generation Singlet ... Berlin, Germany) The cells were seeded in six-well plates (4x105 cells/well) and after 24 h were incubated with MB for 2 h The cells were washed in PBS, removed from the plates using trypsin solution,...

Ngày tải lên: 19/09/2020, 21:32

... vesicles An increase in the 488-2A channel represents an increase in the number of autophagic vesicles staining green Caspase 3 assay Cells were fixed in 4 % paraformaldehyde and washed in permeabilisation ... silencing stathmin 1 (using adenovirus technology) in combination with 5-FU was reported to increase apoptosis and decrease clono-genic survival of prostate cancer cells [25] We therefore examined ... process in which cells self-digest their own organelles, protein aggregates and other macromolecules to main-tain cellular homeostasis However, when a tumour has become established, cancer cells...

Ngày tải lên: 21/09/2020, 11:34

... channels in SW480 cancer cells Keywords: Ca2+; [10]-Gingerol; L-type Ca2+ channel blockers; SW480 cells; Thapsigargin OPEN ACCESS Trang 2Introduction Ginger (Zingiber officinale L Zingiberaceae) ... [Ca2+]i rise in human colorectal SW480 cancer cells In Ca2+-containing medium, the [Ca2+]i rise induced by [10]-gingerol was sustained without a decay during the 5 min of measurements Sustained [Ca2+]i ... of [10]-gingerol-induced Ca 2+ influx by Protein Kinase C A Ca2+ signal could be modulated by the activity of protein kinase C [17,18] The role of protein kinase C in [10]-gingerol-induced [Ca2+]i...

Ngày tải lên: 24/11/2022, 17:48

... glutamine, cysteine, and glycine It is the most abundant intracellular small molecule thiol present in mammalian cells and it serves as apotent intracellular antioxidant protecting cells from toxins ... activated upon binding of a ligand in their extracellular domain, resulting in dimerization of HERs and triggering the intrinsic tyrosine kinase activity of the receptors responsible for a mutual ... agonist in D351G ER mutant T47D:C4:2 cells, as TPEs are unable to increase the luciferase activity in cells expressing the mutant form of ER, indicating the importance of Asp351 of the ER for activation...

Ngày tải lên: 23/03/2014, 17:20

... by ANT isoforms 1 and 2 in the growth of a human glioblastoma cell line (ADF cells) The silencing of ANT2 isoform, by small interfering RNA, did not produce sig-nificant changes in ADF cell viability ... propidium iodide stain-ing (data not shown), indicatstain-ing that the plasma membrane is maintained intact, allowing us to exclude primary necrosis as the principal cell death modality Moreover, ... requires protein synthesis ANT1-silencing induces ADF cell apoptosis and a non-apoptotic cell death modality similar to paraptosis To gain more insight regarding the observed reduction in cell viability,...

Ngày tải lên: 29/03/2014, 09:20

... VX-680 inhibits activation of A and induces monopolar spindle in NB4-R2 cells (A) VX-680 inhibited phosphorylation of Aur-A at Thr288 in NB4-R2 cell line Cells were incubated with increasing doses ... staining NB4-R2 cells were incubated with VX-680 at 2 nM for 24 hr Cells were fixed in cold methanol for 20 min at 4°C and permeabilized in 0.5% TritonX-100 in PBS at room temperature (RT) for ... activity induced defects of mitotic spindle in VX-680 treated cells VX-680 suppresses cell growth and induces cell apoptosis in NB4-R2 cells Next, we studied if VX-680 could suppress proliferation in...

Ngày tải lên: 18/06/2014, 19:20

... cells treated with Cd or parental Jurkat cells treated with either Zn or Cd were minimally affected (Figure 1A) We sought to independently confirm the finding of cell death in JPX-9 cells using ... death in Hct116 cells by Tax + Jun kinase Figure 7 Induction of cell death in Hct116 cells by Tax + Jun kinase Hct116 cells were transfected with pCMV-beta-gal and control plasmid pUC19 or the indicated ... JPX-9 cells To examine the effect of Tax on the growth/death of Jurkat cells, we studied its induction by Zn or Cd in the JPX-9 cell line As mentioned above, JPX-9 is a Jurkat derived cell line in...

Ngày tải lên: 13/08/2014, 13:20

... in HT-29 and SW620 cells than SW480 cells Cancer cells are known to produce higher amount of ROS than normal cells, treating cancer cells with these ROS generating agents would insult these cells ... experimentation was setup for other adenocarcinoma cell lines SW480 PT was found to induce cell death by inducing signifi-cant cell death NAC was found to block total cell death in SW480 cells (Fig.7c-d) ... PT-mediated cell death was studied in meta-static colorectal cancer cell lines SW620 As shown in Fig.2e-f, incubation with 5μM and 10 μM of PT for 24 h induced 24.7 and 41.7% total cell death in SW620 cells...

Ngày tải lên: 06/08/2020, 05:47

... maternal cells However, there are no clinical markers for detecting cell fusion in clinical context Macrophage-specific antigen CD163 expression in tumor cells is reported in breast and colorectal cancers ... both maternal cells CD163 expression in MCF-7 cells could not be induced by paracrine interaction with M2-activated macrophages CD163 positive cancer cells in tumor sections grew in clonal collection ... macrophage traits in cancer cells In the present study, macrophage traits in MCF-7 cancer cells are only generated by fusion with macrophages, proving they are not induced by cellular interaction between...

Ngày tải lên: 22/09/2020, 22:53

... complete results for one cell line as an example Besides the increase in SubG1 peak, in the remaining living cells a decrease in G1 phase and in G2/M-phase and an increase in S-phase of cell cycle ... anaplastic cell lines, increasing the subG1 frac-tion in HTh7 cells by 25.7% and in HTh83 cells by 74.7% In follicular cell lines percentage of subG1 fraction varies from 21.5% in TT2609 to 43.0% in ... kinases in addition to inhibiting AKT and receptor tyrosine kinases Conclusions: Sorafenib inhibited multiple intracellular signaling pathways in thyroid carcinoma cells, which resulted in cell...

Ngày tải lên: 30/09/2020, 11:14

... over-expressed in tumors exhibiting CIN The silencing of these genes caused cancer cell death, suggesting that these genes might be involved in the survival of aneuploid cells In diploid cells, but not in ... selectively elevate CIN in cancer cells to promote cancer cell death identified with this new screening tool could lay the foundation for new treatment strategies for cancer Methods Cell lines Human fibrosarcoma ... HT1080 cells containing the HAC were grown in DMEM medium to 70-80% confluence Metaphase cells were obtained by adding colcemid (Gibco) to a final concentration of 0.05 μg/ml and incubating overnight...

Ngày tải lên: 05/11/2020, 06:32

... important signal involved in Cd-induced cell death in plants [63, 64] It was reported that Cd-induced cell death in suspension cells of Arabidopsis thaliana was accompanied by an increase in H2O2 content ... reports that indicated that H2S in-hibits cell apoptosis by inhibiting Cyt c release, such as in SH-SY5Y cells [70], RGC-5 cells [71] and rat cells [72] Cysteine protease is a kind of biological ... stain dead cells, it is indicative of the level of dead cells As shown in Fig.2a, with increase in treatment duration (0, 12, 24, 36 and 48 h), root tip staining deepened gradually, indicating...

Ngày tải lên: 28/06/2021, 20:32

... Under stressing conditions, a part of MI cells is subjected to PCD while the remaining viable cells differentiate into MII cells In the second phase of PCD, a part of MII cells dies releasing nutrients ... In some cases, bacteria, in response to environmental signals, can induce a death program in neighbouring cells in order to uptake their genetic material (Figure 2b) Indeed in S pneumoniae, cells ... toxin (c) Type III: antitoxin RNA binds and inactivates to the toxin protein (d) Type IV: the antitoxin prevents the effect of the toxin by binding the toxin target Again in response to stress, degradation...

Ngày tải lên: 02/11/2022, 09:24

... p27 found in quiescent cells suggest that the p27 gene also plays a role in maintaining cells in G0 (Hengst et al., 1996) As p27 has a nuclear localization signal in its -COOH terminus, loss ... of our hypothesis in determining clinical significance of cancer stem -cell marks in colorectal cancer Our main objective is to understand the clinical value of cancer stem -cell markers and their ... relevance of cancer stem cells Taking into consideration that the working mechanisms of the cancer stem -cell concept may incorporate many molecular pathways (Barozzi et al., 2002), we have examined a...

Ngày tải lên: 03/10/2015, 20:57

... ATP binding site serves as a docking site for specific binding of cytoplasmic signaling proteins containing Src homology-2 (SH2) and protein tyrosine binding (PTB) domains These proteins in turn ... shock protein 90-binding geldanamycin inhibits cell proliferation, down- regulates oncoproteins, and inhibits epidermal growth factor-induced invasion in thyroid cancer cell line J Clin Endocrionol ... tyrosine kinase inhibitors Inhibiting the activity of tyrosine kinases by low molecular weight compounds capable of interfering with either ligand binding (in the case of receptor tyrosine kinases)...

Ngày tải lên: 03/11/2012, 09:57

... H358), two large carcinoma cell lines (H460, H661), and one squamous cell carcinoma cell line (H226), as well as one small cell lung cancer cell line (SCLC) (H841) were obtained from the American ... SQ participated in acquiring clinical samples and follow-up clinical information MSC participated in study design and coordination, data analysis and interpretation and final writing of the manuscript ... exist within the individual gene promoters for certain genes in lung cancer carcinomas MS-MLPA is only based on a single CpG site compared to an average of 4-6 CpG sites in MS-PCR assays Since only...

Ngày tải lên: 18/06/2014, 16:20