mechanical stretching of cell cultures

... understanding of the mechanical behaviour of the catalyst layers during regular cell operation, mechanical response under steady-state hygro-thermal stresses should be studied under real cell operating ... the influence of the operating parameters on fuel cell performance to investigate the in situ total displacement and degree of the deformation of the polymer membrane of PEM fuel cells ISSN 2076-2895 ... active area of the fuel cell in three-dimensions Nevertheless, except of ref [11], no models have yet been published to incorporate the effect of hygro-thermal stresses into actual fuel cell models...

Ngày tải lên: 05/09/2013, 14:58

... Influence of Pi starvation on growth and secreted AP activity of tomato suspension cells Tomato cells cultured for days in the absence of exogenous Pi had only approximately 40% of the fresh weight of ... 8-day-old +Pi cells (approximately 63 and 25 g of cells were obtained per 500 mL culture of 8-day-old +Pi and –Pi cells, respectively) Eight days following subculture of the tomato cells into –Pi ... concentration of enzyme assayed One unit (U) of activity is defined as the amount of AP resulting in the hydrolysis of lmol of substrate per at 25 °C Phosphatase assay A For routine measurements of AP...

Ngày tải lên: 23/03/2014, 21:20

... Characterization of the cell types and growth kinetics of cultured cells Healthy growing EC and SMC cultures were obtained from UC of group-1 and group-2 individuals No contamination of SMCs in EC cultures ... receiving the impact of the fetal milieu The UC is an exceptional source of vascular cells, which can offer valuable information about the cellular characteristics of the blood vessels of the individual ... 0.005) Several growth parameters of the different cell cultures were analyzed Figure shows the cell proliferation kinetics of the cell types obtained from individuals Cell culture growth follows the...

Ngày tải lên: 18/06/2014, 15:20

... outgrowth assays that evaluate the ability of a fixed input of T cells to inhibit long-term growth of different numbers of target cells, without the addition of cytokines This experimental design ... number of lymphocytes obtained in these cultures As shown in Figure 2, overall we found more pronounced differences in the phenotypic profile of CD8+ and CD4+ T cells prior to the addition of the ... re-stimulation with LCL, CD4+ T cells were adjusted to 1.5 × 106 cells/ml and CD8+ T cells to × 106 cells/ml Cytotoxicity assays Cytotoxic activity of CD4+ and CD8+ T cells was determined in a standard...

Ngày tải lên: 18/06/2014, 16:20

... contents of free (F), soluble ester-bound (SE), cell wall ester-bound (SWE) and soluble glycoside-bound (SG) phenolic acids in control cells of embryogenic cultures of Norway spruce (C) and in the cells ... 8-h level, but levels of the other forms of phenolic acids increased; levels of F by 70% and levels of CWE by 25% (Fig 1) After 24 h of WF treatment a decline in the contents of total phenolics ... doubled content of F after 12 h were observed in WF-elicited cells (Fig 1) In PF-elicited cells, the level of SG increased by about 40% after h After 12  h of treatment with PF the amount of SG was...

Ngày tải lên: 07/08/2014, 10:21

... decreased the number of cells only in P1-F344-cells, but not in P2-F344- and P2-Lewis-cells Proportion of SA-b-gal-positive cells did not correlate with cell number, as this amount of cells was not ... http://www.jeccr.com/content/30/1/102 U/ml Page of 12 50 U/ml a) Total number of cells b) Viable cells c) Dead cells Figure Change in cell number after treatment of F344 and Lewis cells with salivary a-amylase ... (P0) Page of 12 b) Lewis cells (P0) 100 μm 100 μm d) Lewis cells (P1) c) F344 cells (P1) 10 μm 10 μm Figure Differences in cultures of primary mammary cells from F344 and Lewis rats and cellular...

Ngày tải lên: 10/08/2014, 10:21

... method involving rinsing of cell cultures, mainly measured firmly attached cell attachment and viability These results emphasize the importance of consideration of the type of assay utilized in assessing ... cells [29] The detachment of Caco-2 cells from cell culture substrates is therefore possibly due to the onset of apoptosis Lopez et al reported that in the presence of serum, concentrations of ... be a high enough amount of the Cd2+ to cause acute cell death in Caco-2 cells However, the release of a small amount of Cd2+ into the cell culture medium from QDs Page of 15 (page number not for...

Ngày tải lên: 11/08/2014, 00:22

... A B Figure RT-PCR 2of total RNA extracted from hRz-C6/36 cells RT-PCR of total RNA extracted from hRz-C6/36 cells (A) hRz expression in the cells was detected by the presence of an hRz-specific ... quantitation of DENV titer in cellular medium To evaluate the impact of hRzs on assembly and release of DENV, we performed qRT-PCR on the viral RNA extracted from cell supernatant of the infected ... level of specificity of the ribozyme In this study, the length of each arm ranges from 5–10 bp The sequences of hRz templates, positions of the triplets on DENV genome, and the specific strains of...

Ngày tải lên: 12/08/2014, 04:21

... understanding of cellular responses to environmental change Reports of metabolite profiling in the context of defence-related plant secondary metabolism, although rare, include the analysis of elicitor-treated ... the lack of a comprehensive spectral library of standard compounds In this paper, we report the application of 1H NMR to characterize the metabolome of elicitor-induced opium poppy cell cultures ... Biology 2008, 8:5 Results Global metabolite profiling of the elicitation response Aqueous extracts of control and elicitor-treated cell suspension cultures of opium poppy were analyzed in D2O by 1H...

Ngày tải lên: 12/08/2014, 05:20

... of small airway epithelial cells Lineage Lineage verification of small airway epithelial cells A & B; Gene expression of CCSP (CC-10) in small airway (SAEC) cell cultures, large airway cell cultures ... expression of Surfactant Protein-B (SP-B) in small airway (SAEC) cell cultures, large airway cell cultures (LAEC) at p0 and p2 as well as large airway epithelial cell line 16HBE14o- cells as compared ... the five failed cultures was 0.326 ± 0.055 × 106 cells, which is significantly lower than the cell yield for successful cultures (1.071 ± 0.070 × 106 cells; p < 0.01) The presence of BOS significantly...

Ngày tải lên: 12/08/2014, 14:20

... Batch cultures 49 3.1.4 Feeding of batch cultures 49 3.1.5 Rapamycin treatment of batch cultures 50 3.1.6 Determination of cell viability 50 3.1.7 Determination of ... identification of key cell growth genes in recombinant CHO cultures, thereby guiding to the discovery of a new mechanism of the crucial mTOR pathway which paves the way for future improvement of CHO cell cultures ... 154 7.2.5 Single cell cloning of CHO-U19 155 7.2.6 Measurement of targeted pseudouridylation sites 155 7.2.7 Microarrays profiling of snoRNAs in CHO cells 156 Abbreviations...

Ngày tải lên: 08/09/2015, 19:49

... influence of the operating parameters on fuel cell performance to investigate the in situ total displacement and degree of the deformation of the polymer membrane of PEM fuel cells A unique feature of ... active area of the fuel cell in three-dimensions Nevertheless, except of ref [9], no models have yet been published to incorporate the effect of hygro-thermal stresses into actual fuel cell models ... effect of these real conditions on the stresses developed in membrane and gas diffusion layers In addition, as a result of the architecture of a cell, the transport phenomena in a fuel cell are...

Ngày tải lên: 09/09/2015, 10:17

... MECHANICAL CHARACTERIZATION OF BIOPOLYMER AND THE EFFECTS OF MECHANICAL PROPERTIES OF EXTRACELLULAR MATRIX ON CELL BEHAVIORS WONG LONG HUI (B.Eng.(Hons.), ... the cancer cells in 3D cell culture [41,42] The use of 3D biopolymer cell culture has elucidated the importance of generating traction forces through cell- ECM adhesion and degradation of local ... integrity of the impregnated scaffold is also maintained by the interdependence of cell- cell and cell- ECM forces [86] A typical strategy in cell therapy involves impregnating stem cells into...

Ngày tải lên: 09/09/2015, 11:28

... protein-producing cell cultures 25 2.2 Mammalian cell culture for the production of recombinant proteins 25 2.2.1 Accumulation of metabolites in mammalian cell culture medium 26 2.2.2 Induction of apoptosis ... state in cultures of NSO and CHO cells expressing monoclonal antibodies (Ma et al., 2009) Most recently, metabolic profiling with GC-MS enabled the differentiation of baby hamster kidney cell cultures ... mammalian cells include metabolic profiling of media from human hepatoma cell cultures in relation to cell growth (Miccheli et al., 2006), and identifying molecules secreted by human embryonic stem cells...

Ngày tải lên: 11/09/2015, 10:01

... standardized protocol of testing the mechanical behaviour of a hip spacer available, the loading procedure was defined following the recommendations of the ISO 7206 standard for determination of fatigue ... to be used for the creation of finite element models of the femur J Biomech 1996; 29: 1241 20 Cristofolini L, Viceconti M, Cappello A, Toni A Mechanical Validation of Whole Bone Composite Femur ... 23:61-62 18 ISO 7206-4 Determination of endurance properties of stemmed femoral components with application of torsion ISO 1989 19 Viceconti M, Casali M, Massari B, Cristofolini L, Bassini S, Toni A...

Ngày tải lên: 26/10/2012, 09:53

... the balance of expression of members of the Myc/Max/Mad network of proteins (see Fig 1) may dictate the response to loss of Myc (Walker et al 2005) In mice, the effects of deletion of the c-myc ... excision of c-myc (de Alboran et al 2001) Importantly, deletion of the mnt gene, which encodes a member of the Mad family of antagonists of Myc, restores proliferation to c-myc deleted cells, suggesting ... neuronal precursor cells leads to a dramatic loss of proliferative capacity of such cells (Knoepfler et al 2002) As long as questions of redundancy between different members of the myc gene family...

Ngày tải lên: 25/10/2013, 21:20

... in Mouse Models of Cell Cycle Regulators One of the hallmarks of cancer cells is misregulation of cell proliferation Cancer cells are able to evade the normal signals that stop cell division and ... the mechanisms of regulation of normal cell proliferation sets the ground for understanding the deregulated proliferation characteristic of a cancer cell regardless of the type of cancer or where/how ... within certain cell types such as trophoblast giant cells of the placenta and megakaryocytes, but it can also be observed in cells due to a defect in the cell cycle regulation of these cells For example...

Ngày tải lên: 25/10/2013, 21:20

... Behavior of the Very Small • electrons are incredibly small a single speck of dust has more electrons than the number of people who have ever lived on earth • electron behavior determines much of ... see in the properties of the elements Tro, Chemistry: A Molecular Approach The Nature of Light its Wave Nature • light is a form of electromagnetic radiation  composed of perpendicular oscillating ... Characterizing Waves • the frequency, (ν) is the number of waves that pass a point in a given period of time the number of waves = number of cycles units are hertz, (Hz) or cycles/s = s-1 1...

Ngày tải lên: 28/11/2013, 01:11

... and 2.8(f) These may be thought of as the absence of a plane of atoms (intrinsic stacking faults) or the insertion of rows of atoms that disturb the arrangement of atoms (extrinsic stacking faults) ... example of volume defects is presented in Fig 2.9 This shows MnS inclusions in an A707 steel Another example of a volume defect is presented in Fig 1.16(d) This shows evidence of $1–2 vol % of porosity ... approximately 0.3–0.5 of the melting temperature in degrees Kelvin Since the evolution of microstructure is often controlled by diffusion processes, a brief introduction to elementary aspects of diffusion...

Ngày tải lên: 23/12/2013, 00:15