mathematical models and in vitro studies

... treatment were used in the following in vivo and in vitro studies. 3.3 In vitro Study To mimic the functional response of Th17 and other Th cell subpopulations to HDM allergens, an in vitro allergen ... between in vivo and in vitro findings, confirming that not only Th2, but also Th17 cells play an important role in the context of allergic inflammation. IL10 producing CD4+ T cells showed a distinct ... In Vivo and In Vitro Studies of Th17 Response to Specific Immunotherapy in House Dust Mite-Induced Allergic Rhinitis Patients Chun Wei Li1., Han Gui Lu2,3., De Hua Chen2,4, Zhi Bin Lin2,4,...

Ngày tải lên: 23/09/2015, 14:47

... DOX, and their therapeutic effects against the cancer cells were studied in vitro In vivo MSC/Fluc migration in mice having thyroid or breast cancer xenografts was evaluated after systemic injection ... xenograft models and in vitro drug delivery by doxorubicin Senthilkumar Kalimuthu, Liya Zhu, Ji Min Oh, Prakash Gangadaran, Ho Won Lee, Se hwan Baek, Ramya Lakshmi Rajendran, Arunnehru Gopal, Shin Young ... cells was measured by bioluminescence imaging (BLI) Further in vitro migration assays were performed to confirm cancer cells conditioned medium dependent MSC and doxorubicin (DOX) treated MSC migration...

Ngày tải lên: 15/01/2020, 09:12

... encoding reg-ulators of immune and inflammatory responses and also genes involved in apoptosis and cell proliferation [36] In this paper, we report the results of cell-based, in vitroand in silico ... pre-warmed in a water bath at 37C) was dispensed into each well and incubated for 15 min (prewarming fixative is critical to maintaining cell integrity) After 15 min, the plates were inverted to ... future inhibitors designed for this NF-jB signaling pathway should be tested not just in vitro and cell based but also simulated in silico Combining this method of analysis (in vitro, cell-based and...

Ngày tải lên: 16/03/2014, 11:20

... in mediating the effects of FSH on inhibin-A, activin-A, oestradiol and progesterone secretion and also important in mediating IGF-I stimulated inhibin-A, activin-A, follistatin, oestradiol and ... treating bovine theca cells in vitro with control medium or LH (160 pg/ml) in combination with PD98059 (Erk inhibi-tor) and/or LY294002 (Akt inhibiinhibi-tor) on cell number and secretion of androstenedione ... inhibitor) and/or LY294002 (Akt inhibitor) and their effect on cell number and hormone secretion (estradiol, activin-A, inhibin-A, follistatin, progesterone and androstenedione) determined In addition,...

Ngày tải lên: 20/06/2014, 07:20

... Furthermore, increased radiosensitivity in the androgen independent PC cell line PC-3 has been demonstrated in vitro and in vivo [26,27]. Our study analyzes the interactions of irradiation and gen-istein ... estradiol incubation in androgen sensitive LNCaP and androgen independant PC-3 cells in vitro. Clinically relevant irradiation doses between 0 and 4 Gy were tested Methods Cell lines and cultures ... three assays In controls incubated in FCS containing media and in controls incubated in media with FCS-withdrawal there was a induction of p21 expression The same was seen in cells after incubation...

Ngày tải lên: 09/08/2014, 09:22

... insulin signaling upon binding of insulin to insulin receptors causes suppression of gluconeogenesis in liver and. .. catechoamines are released and they inhibit insulin secretion and ... secreted by intestine in response to nutrients ingestion Their main effect is to stimulate pancreas to release insulin after meals intake 6 Two incretin... Activation of insulin signaling is an ... those with diabetes Individuals in this category can either have predominant insulin resistance with relative insulin deficiency or predominant insulin secretory defect with insulin 2 resistance...

Ngày tải lên: 08/09/2015, 18:22

... OF SINGAPORE 2004 Trang 2IN-VITRO STUDIES ON THE COLOR STABILITY AND MASKING ABILITY OF COMPOSITE CORES AND THE INFLUENCE OF POSTS AND CORES ON THE SHADES OF ALL-CERAMIC SYSTEMS SWAMINATHAN ... Meng and Dr.Girija for their sincere support and guidance during the period of my study Trang 4I take immense pleasure in thanking my parents, for constantly encouraging and supporting me in all ... was proposed by CIE in 1931 by defining standard observer and illuminants The first generation instruments could measure point to point reflectance only to be followed by hand calculations to...

Ngày tải lên: 09/10/2015, 11:24

... TNF-induced apoptosis in differentiating myoblasts and prevented the TNF-induced inhibition of myogenesis, as demonstrated by the increased expression of myogenin, myosin heavy chain and caveolin-3, ... Regeneration: in Vivo, in Vitro and in Silico Studies Felicia Carotenuto1,2*, Alessandra Costa3,4, Maria Cristina Albertini5, Marco Bruno Luigi Rocchi5, Alexander Rudov5, Dario Coletti6, Marilena Minieri7, ... apoptosis and preserved muscle myogenesis by increasing the myogenin and alpha myosin heavy chain Moreover, it restored the normal expression pattern of caveolin-3 thereby allowing protein retention...

Ngày tải lên: 15/01/2020, 20:53

... first-order, and Higuchi kinetic models The kinetic model which generates the best fit line was considered In Vitro Skin Permeation Study In vitroskin permeation study was carried out in a diffu-sion (modified ... had not enough con-vincing linearity where the curves were drawn by point to point joining In those cases, best fits of the individual linear graphics were used to determine the respective Kp ... morphology, hardness test, skin irritation test, gel viscosity and swelling index, drug re-lease, in vitro skin permeation study, and in vivo drug plasma profile in rats were performed on the...

Ngày tải lên: 11/05/2020, 12:03

... co-crystallized ligands, thus validating the docking protocol The selected com-pounds showed appreciable binding to the binding site of MMP-2 and MMP-9 proteins as determined by analyzing their bonding interactions ... MMP-2 and MMP-9 proteins by means of Auto-Dock Vina Auto-Docking runs were launched from the com-mand line, followed by the generation and scoring of best nine poses, for each and every ligand using ... visualized, and photo-graphed using a gel documentation system Molecular docking studies The docking studies were performed for selected com-pounds (6, 7, 8, 15 and 16) in the binding site of MMP-2 and...

Ngày tải lên: 29/05/2020, 12:59

... the strength of the protein–ligand interaction, to determine biding poses and free energy values [19] Docking describe ligand binding to a receptor through noncovalent interactions which is commonly ... terpenoids and phenylpropanoids molecules were included in the in vitro evaluation, and QSAR models using genetic algorithms were built to identify molecular and structural properties of biological interest ... (41) Rotundifolone, (42) Sabinene (43) α-Terpinene, (44) γ-Terpinene, (45) 4-Terpineol, (46) α-Terpineol, 47) β-Terpineol, 48) γ-Terpineol, (49) Terpinolene, (50) Thymol Andrade‑Ochoa et al Chemistry...

Ngày tải lên: 29/05/2020, 13:36

... resolution and symmetry of the bands Con-sequently, a wide range of Δ λ (20–140 nm) was investi-gated Good band shapes and adequate sensitivity were obtained upon using Δ λ of 80 and 100  nm in case ... at 389 nm Intraday Interday The second approach SDSF at 346 nm Intraday Interday FDSF at 312.45 nm Intraday Interday Trang 8for ADP in a good agreement with the label claims and no interference ... in adapalene gels using high performance liquid chromatography Se pu 26(5):640–642 8 Ruhl R, Nau H (1997) Determination of adapalene (CD271/Differin) and retinol in plasma and tissue by online...

Ngày tải lên: 29/05/2020, 14:19

... terminated Second is the inhibition of chain initiation by scavenging reactive oxygen and nitrogen species.35 In the present investigation, lipid peroxidation was induced in RBCs by t-BHP The inhibition ... hydrazine method The reference standard used in this assay was naringenin Five hundred, 1000, 1500 and 2000µg of naringenin and 100 – 1000µg of extracts were made up to 1.0 mL with methanol in separate ... composition and in vitroantioxidant studies on Syzygium cumini fruit Agro-Processing and Natural Products Division, Regional Research Laboratory, CSIR, Trivandrum-695 019, India Abstract: Syzygium cumini,...

Ngày tải lên: 18/11/2022, 07:33

... sequencing andin vitro expression of PCR products 5.1 Introduction Analysis of PCR products is critical in optimizing PCR conditions to yield reliable and accurate results, and in interpreting the ... separation in thin-coated capillaries containing nonpolymerized gel matrices and laser detection systems The introduction and removal of polymer from the capillaries, plus loading and running samples and ... (Chapter 3) DNA sequencing chemistry and automation Dideoxy terminator DNA sequencing (5) involves the incorporation of 2′,3′ -dideoxynucleotide ‘terminators’ into nascent DNA chains (5; Figure 5.3)....

Ngày tải lên: 25/10/2013, 22:20

... an in vitro substrate for the PsCCaMK, the effect of phosphorylation on its DNAbinding property was tested The protein was in vitro phosphorylated using cold ATP and used for DNA binding studies ... protein kinases are involved in stress signaling pathways [29] and recent work points towards the involvement of Ca2+/CaM-dependent protein kinases in stress signaling [16], changes in the level of ... containing lg purified p40 (fraction A) and those containing lg of both p40 and p72 PsCCaMK kinase (fraction B) were used for in vitro phosphorylation in the absence or presence of Ca2+, CaM, kinase...

Ngày tải lên: 18/03/2014, 01:20

... synthesis, and metal loading RP conceived of the study, and participated in its design and coordination, carried out in vitro toxicity studies, SEM and EDX analysis and drafted the manuscript. Competing ... synthesis, characterization, and metal binding properties of our Cu-binding particles, as well as preliminary in vitro toxicity in cancer cells Results Synthesis and characterization of Cu-loaded ... antiproliferative effect and intracellular glutathione depletion induced by Casiopeína IIgly and cisplatin in murine melanoma B16 cells Toxicol in Vitro 2011, 25:868-873. 36 Alemón-Medina R, Breña-Valle...

Ngày tải lên: 21/06/2014, 02:20

... cell line in vivo and in vitro Based on these results, we hypothesized that the two different tumor cell lines, including GBC-SD and SGC-996, can exhibit significant different invasive ability and ... were incu-bated with 0.5% PAS for 10 min in a dark chamber and washing with distilled water for 3 min, finally all of these sections were counterstained with hematoxylin The Microvessel in marginal ... and their invasive potential to participate in vessel-like structures forma-tionin vitro, and succeeded in establishing GBC-SD and SGC-996 nude mouse xenograft models In addition, highly invasive...

Ngày tải lên: 10/08/2014, 10:21

... cytokine repertoire of DC matured in the presence of inflammatory stimuli com-prises pro-inflammatory cytokines and chemokines, including the T cell inhibitory cytokine IL-10, the Th-1 promoting ... PerCP-Cy5.5 and CD4 APC and acquired on a flow cytometer CD3+CD4+ lymphocytes were gated including the blasts and excluding CD209+ MDDC The percentage of cells showing decreased CFSE staining intensity ... IL-12+ and COX-2+cells, compared to the corresponding unstimulated cells (p < 0.05, data not shown) The dot plots in Fig 3A and 3B show the intracellular staining profiles of IL-12 and COX-2 in...

Ngày tải lên: 11/08/2014, 10:23

... nausea and vomiting that can be quite severe and require interrupting treatment A less frequent side effect is bone pain[47] Other rare side effects include vaginal bleeding, thrombophlebitis, and ... is excreted in the bile and eliminated through the faces, with small amounts eliminated in the urine[44, 78, 83] Following oral administration of tamoxifen, elimination is biphasic and is dependent ... from the gastrointestinal tract[69] Its bioavailability is high (approximately 100%) and independent of dose, suggesting minimal first-pass metabolism[70, 71] Following single-dose administration...

Ngày tải lên: 09/10/2015, 11:24

... curcumin and capsaicin H Manjunatha and K Srinivasan Table Effect of dietary curcumin and capsaicin on iron(II)-induced (in vivo) and copper-induced (in vitro) LDL oxidation in rats Rats were injected ... capsaicin (saline-injected); 6, capsaicin (Fe2+-injected); 7, curcumin + capsaicin (saline-injected); 8, curcumin + capsaicin (Fe2+-injected) 4530 on curcumin, capsaicin or curcumin + capsaicin, the anodic ... curcumin and capsaicin H Manjunatha and K Srinivasan Protective effect of dietary curcumin, capsaicin and their combination on iron-induced LDL oxidation in vivo and copper-induced LDL oxidation in...

Ngày tải lên: 08/03/2014, 08:20