lysosomal proteases revival of the sleeping beauty

... in the presence of CHX, the fatty acid bypasses the absence of ACS4 but not the absence of StAR Then, the partial inhibition produced by CHX on AA stimulated steroidogenesis was unexpected The ... explain the mechanism by which in these situations there is a remaining 20% of steroid synthesis, due to the direct effect of AA ⁄ AA-CoA produced within the mitochondria by the action of ACS4 ... feature in the regulation of cholesterol transport by AA and the release of AA in a specialized compartment of the cells could offer novel means for understanding the regulation of steroid synthesis,...

Ngày tải lên: 16/03/2014, 12:20

Ngày tải lên: 13/11/2014, 10:36

... inner diameter of 50 mm, outer diameter of 60 mm, and a height of 20 mm (Fig 2) This structure exposes 18 mm of the point of the current supply electrode and 12 mm of the point of the voltage measurement ... measurement electrode while insulating the other parts, and measures the electric conductivity of the soil in the center of the column The inner and outer diameters of the stainless steel rods were mm ... early leaching of salts in the case of the 0.5 wt% application The results of the lower sensor showed that most of the salts in the soil could be leached in PV and after that the ECv only decreased...

Ngày tải lên: 05/09/2013, 09:38

... fracture The first spatial moment (M1) describes the displacement of the centre of the mass and the second spatial moment (M2) describes the spread of the deviation from the centre of mass The expressions ... at the inlet as well as the outlet of the fracture The results of the numerical model for the radionuclides in a fracture-matrix coupled system are compared with the analytical solutions of Sudicky ... progresses the velocity profiles merge with each other as the concentration of radionuclide gradually reduces along the length of the fracture due to matrix diffusion, sorption of the fracture...

Ngày tải lên: 05/09/2013, 17:03

... action on the ground The women supported the idea of community village policing and some of them in fact indicated that they (if they had the time) would be willing to be police reservists They also ... evaluating the impact of the Tshitwe Road Upgrading Project on the lives of the men and women of Tshitwe, Mahapa and Mashiri (2001) used an activity-based approach The authors noted that while the project ... redistribution of these resources back to the rural sector, compared to women who used their money to purchase local goods and services With regard to the impact of the road upgrade on the travel demands of...

Ngày tải lên: 15/02/2014, 01:20

... as the pollutant of interest Of all of the modes of commuting, the highest mean exposures were experienced by the motorcyclist This is consistent with the results of other studies found in the ... along part of their journey While these are effective for the portions of the commute where they exist, in the city where pedestrians and cyclists merge with the motorised traffic, the highest ... Because of bus route constraints, the bus commuter had a different end point than the other commuters but the journey distance was the same (see Fig 1) Route Commuting from the north of the city...

Ngày tải lên: 18/02/2014, 04:20

... and k0 are the inactivation rate constants either in the presence or in the absence of various concentrations of a ligand A, k2 and k1 are the rate constants of the inhibition of the bilitranslocase– ... in Fig 5B n, Number of [A] tested; k2 ⁄ k1, the value of the intercept in the Scrutton and Utter plot, where k2 and k1 are the rate constants of the inhibition of either the bilitranslocase-ligand ... provoke an 3284 Fig The dependence of valinomycin-induced disappearance of BSP on the osmolarity of the extra-vesicular medium in the presence of carnation petal microsomes The assay was carried...

Ngày tải lên: 20/02/2014, 01:20

... cells [24–26] As the action of arsenite involves the modification of a limited number of arsenite-sensitive target proteins, we hypothesized that elucidation of the mechanism of arsenite-induced ... increases the uptake of 2-DOG without the need for PI-3¢ kinase activity as judged from the absence of an effect of arsenite on PI-3¢ kinase activation and the lack of inhibition by either wortmannin ... downstream of PI-3¢ kinase involving the activity of atypical PKC-isoforms, however, additional factors contribute to the level of arsenite-induced GLUT4 translocation and the magnitude of glucose...

Ngày tải lên: 21/02/2014, 00:20

... for the transport of lipids to this membrane [27] The yeast member of the family Tob55 ⁄ Sam50 is part of the Tob ⁄ Sam complex and is involved in the insertion of b-barrel proteins into the ... involved in the import of photosynthetic proteins However, the reduced accumulation of photosynthetic proteins is also tied to a reduction in the expression of the corresponding genes [17,65] Therefore, ... [17,65] Therefore, the extent of the physical involvement of the two receptors, atToc33 and atToc159, in the translocation of the photosynthetic preproteins (down-regulated in the mutants) is unclear...

Ngày tải lên: 07/03/2014, 03:20

... which anchor the protein in the membrane The position and function of the long C-terminal tail on the other hand remains a matter of controversy [10,11,13–15] According to one hypothesis, the hydrophilic ... because the demand of the chloroplast is ‘sensed’ inside the organelle, the IEM is closest to the origin of the signal, and thus regulation at the Tic complex could be one of the fastest ways to ... is the recruitment of FNR from the stroma or even thylakoids to the Tic complex in order to become part of the hypothetical electron transfer chain mentioned above However, the involvement of the...

Ngày tải lên: 07/03/2014, 03:20

... transported by the Tat pathway contain a characteristic pair of arginine residues in the N-terminal region of the signal peptide, which gives the pathway its name The Sec pathway The chloroplast ... insertion of the substrate into the lipid bilayer and subsequent interaction with the Tat translocase may take place only in later stages of the translocation process [35] Analysis of the chimeric ... polypeptide, which consists of the transit peptide from OE16 fused to the mature OE23 protein, presents an alternative model for the interaction of the preprotein with the receptor The 16 ⁄ 23 chimera...

Ngày tải lên: 07/03/2014, 03:20

... an outline of the future challenges in this field is provided, and the insights that may be gained from a greater understanding of the mechanism of dual targeting for the targeting of location-specific ... albeit the mature protein may affect the efficiency, the effect of the mature protein on targeting appears to be more pronounced in the case of dual-targeted proteins This likely reflects the fact ... to both locations with the same passengers [31] The properties of targeting of Arabidopsis histidyl-tRNA synthetase was intermediate between these two extremes, whereas the mitochondrial passenger...

Ngày tải lên: 07/03/2014, 03:20

... in the estimation of the signal being tracked depends on the reporting frequency rate used by the sensor nodes sending their readings to the sink in the decision interval The variation of the ... with each of these network states Consequently, the sink takes the required action based on the priority of the network states of the multiple concurrent events be the number sharing the same ... of The values of , , and are given in Table I and on Fig and Hence, the number of packets sent with the reporting frequency of in each decision interval of can be calculated by Therefore, the...

Ngày tải lên: 16/03/2014, 19:20

... deflection of the flow by pore walls plays the role of the stirring, but unlike the case of the teacup the pore walls also slows down the diffusion The net effect is nevertheless an enhancement of the ... are of interest The first is the flow of the fluid itself, and the second the transport of a solute introduced into the flow at a specific point in space The fluid flow problem is usually one of ... distribution of tracer concentration showed the influence of the medium on the flow of the tracer This result is remarkable, since the presence of the grains or pore walls that make up the medium...

Ngày tải lên: 22/03/2014, 15:20

... in the intermembrane space between the outer and the inner envelope Tic22 is a subunit of a soluble intermembrane space complex, which facilitates the transfer of preproteins from the Toc to the ... indicating that the rate of translocation exceeds the rate of processing for pTic22 The pTic22 translation product is completely degraded by the protease thermolysin, indicating that the precursor ... (Fig 1A, lanes 4–6) The import of QORH into the inner envelope of chloroplasts was shown to depend on targeting information present in the C-terminus of the preprotein [11] Therefore, we constructed...

Ngày tải lên: 23/03/2014, 07:20

... Uptake of [3H]cGMP in the absence of ATP but in the presence of the nonhydrolysable ATP analogue, ATP-c-S, was less than 5% of the uptake in the presence of ATP The amount of [3H]cGMP taken up by these ... compared using an F-test on the ratio of the variance associated with the reduction in degrees of freedom to the variance of the fit with the smaller number of degrees of freedom [33], VR ¼ ðSSE2 ... Uptake of the substrate was expressed relative to the protein concentration of the membrane vesicles, and all data were corrected for the amount of radiolabelled substrate bound to the filter in the...

Ngày tải lên: 23/03/2014, 21:20

... superlattices may improve the thermoelectric figure of merit The results of further studies should also elucidate the complex physics of transport in these artificial materials ACKNOWLEDGMENTS The authors would ... temperature thermal conductivity of 0.92 0.12 W/m-K The cross-plane thermal conductivity in the temperature range of 300 K–380 K is shown in Figure The cross-plane thermal conductivity indicates that the ... extract the effective thermal activation energy of 114 6 meV The modification of the room temperature barrier (AB) height by varying the doping levels (i.e., by growing at high oxygen pressure) of the...

Ngày tải lên: 06/05/2014, 08:53

... exponentially until they reach the center of the barrier, and then they grow until they reach the metallic interface on the other side Since the wavefunction connects the two metallic leads, the electrons ... physicists at the banquet of the annual meeting of the American Physical Society The title of his talk was "There's plenty of room at the bottom" [Feynman (1961)] There was much conjecture amongst the ... approaches the interface with the barrier, it meets a hole in the metal, which annihilates one of the electrons, while the other electron moves through the barrier to the next interface There, the electron...

Ngày tải lên: 04/06/2014, 14:33

Ngày tải lên: 04/06/2014, 14:41