liposome dna complexes and their interaction with cells

... Visualizing the interactions between circular oligomers and TBA2: Au-TBA2 with (a) thrombin only, (b) with both thrombin and 1, (c) with both thrombin and 9a, (d) with both thrombin and 10b, (e) with both ... akin to their binding toward TBA Figure 7. 15 Visualizing the interactions between circular oligomers and TBA2: Au-TBA2 with (a) thrombin only, (b) with both thrombin and 1, (c) with ... due to the nonspecific interactions between lysozyme and DNAs attached to the AuNPs. With the addition of various pentamers into the solution containing DNA-modified AuNPs and lysozyme, AuNPs aggregation

Ngày tải lên: 10/09/2015, 15:49

... framework for characterizing e-health users and their interaction with ehealth systems Ole Norgaard University of Copenhagen, Denmark Dorthe Furstrand University of Copenhagen, Denmark Danish ... characterizing e-health users and their interaction with e-health systems Ole Norgaard Department of Public Health University of Copenhagen, Denmark E-mail: ole@norgaard.dk Dorthe Furstrand Department of ... e-health research and development there is a need for a broader understanding of the capabilities and resources required for individuals to use and benefit from e-health services, i.e their e-health

Ngày tải lên: 15/01/2020, 00:06

... variants and traits related to glucose metabolism and their interaction with obesity on breast and colorectal cancer risk among postmenopausal women Su Yon Jung1*, Eric M Sobel2, Jeanette C Papp2 and ... self-questionnaires on demographic and lifestyle factors and their medical and reproductive histories Anthropometric measurements, including height, weight, and waist and hip circumferences had been ... for their associations with glucose metabolism and breast cancer and CRC, we selected 29 final variables (Table 1) for this study after performing univariate and stepwise regression analyses and

Ngày tải lên: 06/08/2020, 07:48

... microscopy, alongside tensiometry and AFM, to investigate plasma proteins and their interactions with polymers at the AWI, enhancing both methodological approaches and the understanding of interfacial behavior ... phospholipoproteins and lipids, reduces surface tension at the AWI of alveoli, and its deficiency can lead to alveolar collapse and respiratory failure.Plasma proteins and their interactions at the ... synthetic polymers in biomaterials, biosensors, and drug delivery has made it crucial to understand and control the interactions between synthetic polymers and proteins at interfaces Recent advancements

Ngày tải lên: 24/07/2023, 06:47

... woodchucks infected with woodchuck hepatitis virus (WHV), and with pre-existing liver tumors, were used as a model to investigate if complexes of cationic liposomes and non-coding DNA (JVRS-100) were ... liver tumor formation in woodchucks with established hepatocellular carcinoma by treatment with cationic liposome-DNA complexes Jeffery Fairman1,2, Katherine H Liu3 and Stephan Menne3,4* Abstract ... then treated with DNase I (Invitrogen) and reverse transcribed to complementary (c) DNA with MultiScribe Reverse Transcriptase (Applied Biosystems) using oligo(dT) Triplicates of cDNA were amplified

Ngày tải lên: 20/09/2020, 01:39

... between CD4 count and vitamin A concen-tration Individuals with CD4 counts <200 cells/mm3 had lower vitamin A levels compared with those indivi-duals with 200-499 cells/mm3and >499 cells/mm3 The ... adduct levels and vitamins A and E concentrations in the plasma of positive and HIV-negative Ghanaians and examined the association of vitamins A and E with HIV status, aflatoxin levels and hepatitis ... dried grains and nuts under hot, humid and unsanitary conditions Acute and chronic exposures to aflatoxins compro-mise immunity and enhance macro- and micro-nutrient malnutrition and neonatal

Ngày tải lên: 20/06/2014, 08:20

... were searched for using phase contract microscopy of tissue and haemolymph, and smears of flies after fixation in methanol and staining with 5 p 100 solution of Giemsa for 12 hours The presence ... (N kingi and DCV), (SimES-st stock) Some parameters of fitness (fecundity, fertility and rate of development) were measured for both S and wild-type flies from the same parents (S X S and + X ... hereditary virus with low pathogenicity. II Material and methods Drosophila strains The strain in which the S character appeared was denominated SimES ; it was already described (C , 1980 a and b) A

Ngày tải lên: 09/08/2014, 22:22

... 78.7.6%, and 95.1% for EFB, SP, SS, PY, BY, and TSW, respectively (Table 3) Significant positive phenotypic and genetic correlations between EFB and SP and between yield and silique traits (SP and ... demonstrated that SUT co-localizes with yield-related quantitative trait loci This paper reports the isolation of BnA7.SUT1 alleles and their promoters and their association with yield-related traits ... Of interest, the SNP sites (s60 and s222) from the promoter and s1448 from the exon of BnA7.SUT1 were associated with EFB and TSW and explained an average 12% and 11% of phenotypic variation

Ngày tải lên: 11/08/2014, 11:21

... distribution, and reproduction in any Research article Dissecting grain yield pathways and their interactions with grain dry matter content by a two-step correlation approach with maize seedling ... COMMUNICATIO N/SIGNAL TRANSDUCTION MECHANISM CELL RESCUE, DEFENSE AND VIRULENCE INTERACTION WITH THE CELLULAR ENVIRONMENT INTERACTION WITH THE ENVIRONMENT BIOGENESIS OF CELLULAR COMPONENTS DG, ... pathways and biological processes were elucidated Genes involved in sucrose degradation and glycolysis, as well as genes involved in cell expansion and endocycle were found to be associated with

Ngày tải lên: 12/08/2014, 03:21

... acceptance. Fully formatted PDF and full text (HTML) versions will be made available soon. Getting out and about in older adults: the nature of daily trips and their association with objectively assessed ... distribution, and reproduction in any medium, provided the original work is properly cited. - 1 - Getting out and about in older adults: the nature of daily trips and their association with objectively ... PA. Purpose: to describe the frequency, purpose, and travel mode of daily trips in OAs, and their association with participant characteristics and objectively-assessed PA. Methods Participants

Ngày tải lên: 14/08/2014, 08:21

... associated with schizophrenia and autism(reviewed in [38]), and may also correlate with milk allergy [39,40] in humans The high degree of LD between SNPs allowed us to con-struct haplotypes within and ... CSN1S1, CSN2 and CSN1S2 genes and investigate associations between haplotypes and DYDs for protein yield and milk yield Analysis for CSN2 reveals two haplotypes (2 and 5) that associate with low ... indicating which haplotypes and how many copies are carried by the son; and b is a column indicating the random effects of the haplotypes; ai is a random polygenic effect of son i, and ei is a residual

Ngày tải lên: 14/08/2014, 13:21

... during, and after a test, then further investigated any correlation with listening test performances, and finally a student report was used to examine how HLP and LLP students adjusted their strategy ... upper-secondary school students’ test-taking strategies and their relationship with listening performance It further looks into how students adjust their strategies under different task conditions The ... and discussion, and recommendations Chapter one, literature review of listening comprehension problems, listening comprehension strategies and Listening Task in Relation to Task Performance and

Ngày tải lên: 10/08/2015, 19:48

... known modes of a-amylase inhibition A standard colouring scheme is used with enzyme drawn in magenta and inhibitor helix, strand and coil drawn in red, cyan and yellow, respectively The calcium ... activity PPA and HSA 0.19 T aestivum PPA and HSA 0.53 T aestivum HSA and PPA (low) 0.28 WRP25 T aestivum T aestivum PPA and HSA None WRP26 T aestivum None WRP27 T aestivum None 1,2 and BIII S cereale ... 1) and forms a separated folding unit, exclusively made of b sheet Eight of the 10 strands fold into a b sandwich structure with ÔGreek keyÕ topology The conservation of the interface of A and...

Ngày tải lên: 21/02/2014, 03:20

... maps, with the o and coot programs [36,37] The final refinement was performed with refmac from the ccp4 suite [32], with TLS parameterization Both cns and refmac used the same Rfree set (randomly ... dithiothreitol and mm EDTA, and purified further by anion exchange chromatography with a Mono Q column (Amersham Biosciences; 20 mm Tris ⁄ HCl, pH 7.5, containing 10 mm dithiothreitol and mm EDTA, with ... [16] Additionally, the bands containing the RI–RNase A or RI–SGRSGRSG-RATE complexes (Fig 4, lanes and 4) were excised and subjected to SDS ⁄ PAGE, in which the noncovalent complexes dissociate...

Ngày tải lên: 22/03/2014, 16:21

... and their abiotic analogues) Generally, single-stranded abiotics are unnatural backbones that mimic secondary structures, such as folding and helices, while multi-stranded foldamers ... flexibility of the linkers and various nonconvalent interactions that enforce discrete folded conformation Considering these advantages, single-stranded foldamers with various abiotic backbones ... solvophobic interaction, 7 hydrogen-binding interactions8 and metal coordination.9 However, the challenges in designing synthetic folding oligomers exist in terms of folding stability, tunability, and...

Ngày tải lên: 10/09/2015, 15:49

... stacking and van der Waals’ interactions provide the driving forces for the observed formation of columns With their persistent shapes, tunable sizes and tendency to aggregate into column- and channel-like ... between axis ac and plane of the molecules, all the other three dimers (2a, 2c and 2d) are stacked along axis a with angles of 90º, 53º and 70º, respectively, between axis a and plane of the ... from 3a and generated by breaking H-bond of Oa···Hb (1A & 1B), Hb···Oc (2A & 2B), Oc···Hd (3A & 3B) and Hd···Oe (4A & 4B), and by breaking H-bonds of both Oa···Hb and Hb···Oc (5A & 5B), and both...

Ngày tải lên: 10/09/2015, 15:49

... slow evaporation of 5a and 6a in mixed solvents containing hexane and chloroform 91 (1:1 v/v) for 5a and hexane and dichloromethane (1:1 v/v) for 6a at room temperature Their crystal structures ... contact between proton and proton 24 in 6c (Figures 3.5d and 3.5e), we decided to replace the methyl side chain at the nitro end with an isopropyloxy side chain to generate 5b and with an octyloxy ... protons and their adjacent interior methoxy protons, (f) Chemical structure of 6b More NOESY, ROESY and TOXSY studies of 6b were demonstrated in Figure 3.8 and Figure 3.9 Figure 3.8c and 3.8d...

Ngày tải lên: 10/09/2015, 15:49

... mL) and the mixture was heated under reflux for h The solvent was removed in vacuo and anhydrous ether (2 x mL) was added and removed again The solvent was then removed in vacuo and saturated with ... The mixture was heated at 50 0C overnight and then quenched 126 with water (4 ml) The aqueous layer was neutralized with 1M KHSO4 (0.4ml) and extracted with DCM (2 x mL) Removal of CH2Cl2 solvent ... (1.5 mL) and the mixture was heated under reflux for h The solvent was removed in vacuo and anhydrous ether (2 x 30 mL) was added and removed again The solvent was then removed in vacuo and saturated...

Ngày tải lên: 10/09/2015, 15:49

... been used very often in quadruplex-targeting ligands to enable the ligand to efficiently interact with the grooves and loops of quadruplex DNA and the negatively charged backbone phosphates In ... telo23 DNA sequence.33 In the study of G-quadruplex structure, effective assays are required for studying the G-quadruplex formation and for determining the interaction between ligands and G-quadruplexes ... inhibit the action of the DNA polymerase.41 The single-stranded DNA could be induced into a G-quadruplex structure that blocks hybridization with a complementary strand overlapping the last G...

Ngày tải lên: 10/09/2015, 15:49

... (15-mer) and TBA2 (29-mer) and their analogues were chemically coupled to AuNPs via the formation of Au-S bond Table 6.1 lists all the DNA- modified AuNPs AuNPs heavily loaded with linear DNA strands ... both Au-T6 and thrombin Our earlier observation and discussion in the duplex approach shows that the interaction of TBA with its complementary DNA strand is stronger than that between TBA and thrombin ... shorter complimentary DNA strand containing a region of about 12-20 nucleotides complementary to DNA apamer and (3) gold nanoparticles (13 nm in diameter) functionalized with DNAs that enable the...

Ngày tải lên: 10/09/2015, 15:49