... unidentified ninhydrin-positive component suggest that the cell wall contains other polymer(s) in addition to ribitol teichoic acid The polymers present in the cell wall were investigated using NMR ... of plant pathogenic streptomycete strains causing scab diseases of potatoes and root crops Further studies of the cell wall anionic polymers in the plant pathogenic streptomycetes, including ... above, the Kdn-containing polymer was also revealed in the cell wall of a streptomycete strain isolated from common scab lesions of the potatoes [3], which induced scab disease in potato tubers,...
Ngày tải lên: 17/03/2014, 10:20
Recent Advances in Plant Biotechnology
... and including two additional components, the joining chain (blue disc) and the secretory component (green disc) Heavy chains are shown in gray and light chains in pink Solid black lines indicate ... and potentials of inverse metabolic engineering of cell types that not normally contain such systems (bacteria, yeast, and plants) in conjunction with creatine or arginine kinase systems (Sauer ... attached to virtually any protein of interest and still fold into a fluorescent molecule Fluorescent proteins are increasingly being employed as noninvasive probes in living cells due to their ability...
Ngày tải lên: 02/10/2012, 10:48
... as pancreatic carcinoma cells, lung cancer cells [7, 10 ], prostate cancer cells [24] and renal carcinoma cell line [28, 19, 44 ] Other interesting finding is that the cancer cells resistant to ... persistently HBV expressing cell line HepG2.215 and its parent cell line, a non-HBV expressing cell line HepG2 We found that cIAP1 and cIAP2 were clearly increased in the HBV expressing cells but XIAP ... main well-characterized mechanism is the inhibition of active Int J Med Sci 2005 2(1) 34 caspases by the direct binding of IAPs In the many case, for example, in the lung carcinoma cells, the interaction...
Ngày tải lên: 02/11/2012, 11:17
Tài liệu In - plant transportation doc
... PB-Nr: M06.01.01 In- plant Transportation Issued by Date Hr Bauer Version 00 Page 2/2 ...
Ngày tải lên: 18/01/2014, 15:20
Tài liệu Báo cáo khoa học: Identification and characterization of the transcription factors involved in T-cell development, t-bet, stat6 and foxp3, within the zebrafish, Danio rerio docx
... implicated in other protein–protein interactions, a STAT protein DNA-binding domain and an SH2 domain, which binds phosphorylated tyrosine residues in the context of a longer peptide motif within a ... STAT protein all-alpha domain, STAT protein DNA-binding domain and SH2 domain of stat6, and the zinc-finger domain, leucine-zipper domain and fork-head domain of foxp3 In addition, for stat6 and ... domains, including an N-terminal STAT protein interaction domain, which strengthens interactions between STAT dimers on adjacent DNA-binding sites, a coiled-coil STAT protein all-alpha domain,...
Ngày tải lên: 16/02/2014, 09:20
Tài liệu INNOVATIONS IN STEM CELL TRANSPLANTATION doc
... transplanted stem cells in the host In conclusion, Cell Transplantation is bridging cell transplantation research in a multitude of disease models as methods and technology continue to be refined ... cell or a brain cell During this last decade, the number of published articles or books investigating the role of stem cells in cell transplantation or regenerative medicine increased remarkably ... sibling hematopoietic stem cell transplantation On applying KIR genotyping, some studies investigated the effect of KIR in sibling HSCT A study of 220 donor-recipient pairs in HLA-matched sibling...
Ngày tải lên: 18/02/2014, 04:20
Tài liệu Báo cáo khoa học: Down-regulation of heme oxygenase-2 is associated with the increased expression of heme oxygenase-1 in human cell lines docx
... HepG2 cells were maintained in Dulbecco’s modified Eagle’s medium (Sigma) YN-1 cells were maintained in Iscove’s modified Dulbecco’s medium (Sigma), and K562, KG1 and Jurkat cells were maintained in ... maintained in HO-2– ⁄ – mice through appropriate resetting of HO-1 expression In contrast with HO-1 expression, expression of HO-2 mRNA and protein was not increased in several human cell lines ... Human cell lines used were HeLa cervical carcinoma cells, HepG2 hepatoma cells, K562 and YN-1 erythroleukemia cells, Jurkat T-lymphocyte cells, KG1 myeloid cells, H146 small cell lung cancer cells,...
Ngày tải lên: 19/02/2014, 05:20
Tài liệu Báo cáo khoa học: Separation of a cholesterol-enriched microdomain involved in T-cell signal transduction doc
... This is in remarkable contrast to cell binding by filipin, another cholesterol-binding reagent Filipin staining is significantly retained under the same depletion conditions [19] Thus BCh binds to ... localized in BCh-bound membranes as judged by the binding of cholera toxin (Fig 3B, CTX) It has been reported that several proteins participating in T -cell signalling are enriched in lipid rafts ... Phosphoprotein associated with glycosphingolipid-enriched microdomains (PAG), a novel ubiquitously expressed transmembrane adaptor protein, binds the protein tyrosine kinase Csk and is involved in regulation...
Ngày tải lên: 20/02/2014, 03:20
Tài liệu New Advances in Stem Cell Transplantation Edited by Taner Demirer ppt
... RNA binding protein and stem cell marker Inhibitor of microRNA processing in embryonic stem (ES) and carcinoma (EC) cells Table Combinations of the genes that have been used to obtain the induced ... medicine or reparative medicine During this last decade, the number of published articles or books investigating the role of stem cells in cell transplantation or regenerative medicine increased ... factors into somatic cells By using these viral delivery systems, 12 New Advances in Stem Cell Transplantation Fig Methods for producing induced pluripotent stem cells (iPSCs) by non-integrating...
Ngày tải lên: 20/02/2014, 08:20
Báo cáo khoa học: Expression of cholinesterases in human kidney and its variation in renal cell carcinoma types pdf
... explain their wide distribution in tissues and cells [16], including stem cells [17] Increasing evidence links these noncatalytic actions with the binding of cholinesterases to several protein ... between acetylcholinesterases of kidney and erythrocytes in the extent of binding with the lectins concanavalin A, Lens culinaris agglutinin 4522 (LCA), and Ricinus communis agglutinin (RCA) (Fig ... acetylcholinesterase can interact with laminin and collagen IV [17,18], nicotinic receptors [19], amyloid b-peptide and presenilin-1 [20], neuronal enolase, the scaffold protein RACK1 and protein kinase...
Ngày tải lên: 06/03/2014, 22:21
Báo cáo khoa học: Transient potential receptor channel 4 controls thrombospondin-1 secretion and angiogenesis in renal cell carcinoma ppt
... expressed in HNK or RCC cells (Fig 3A,B); thus, there was no functional redundancy HNK cells expressed high levels of the calcium-binding protein, calbindin D28K [28] (Fig 3C) In normal kidney, calbindins ... of such inhibitors in angiogenic tumors impedes their progression: these include angiostatin, endostatin, tumstatin, PEDF, SPARC (secreted protein, acidic and rich in cysteine), tissue inhibitor ... Functions of the conserved thrombospondin carboxy-terminal cassette in cell extracellular matrix interactions and signaling Int J Biochem Cell Biol 36, 1102–1114 25 Dinser R, Zaucke F, Kreppel F, Hultenby...
Ngày tải lên: 07/03/2014, 05:20
Báo cáo khoa học: Plant DNA polymerase k, a DNA repair enzyme that functions in plant meristematic and meiotic tissues docx
... may be important for plant cells On the other hand, the N-terminal BRCT domain proposed to mediate protein–protein interactions involved in DNA repair and cell cycle checkpoint regulation on DNA ... coding region was cloned into the pET21a expression vector (Novagen), which was transfected into BL21 (DE3) (Novagen) bacteria in 500 mL Luria–Bertani medium containing 50 lgÆmL)1 ampicillin Cells ... were introduced into OsPol k by a PCR-based method as described previously [16] Overexpression of OsPol k protein in Escherichia coli The OsPol k protein, which contained a six-histidine C-terminal...
Ngày tải lên: 07/03/2014, 15:20
Báo cáo Y học: Transient activation of the c-Jun N-terminal kinase (JNK) activity by ligation of the tetraspan CD53 antigen in different cell types pptx
... appears to be independent of the cell type, and thus of cell- specific protein interactions MATERIALS AND METHODS Cell lines The rat IR938F, a pre-B cell lymphoma, and the human Jurkat cell line, derived ... proteins containing the GAL4 DNAbinding domain and the c-Jun activation domain (residues 1–223) in wild-type and mutant forms (not phosphorylatable by mutation of both serines 63 and 73 to alanine) ... Co-stimulation of T cells with CD2 augments TCR-CD3-mediated activation of protein tyrosine kinase p72syk, resulting in incresed tyrosine phosphorylation of adapter proteins, Shc and Cbl Int Immunol...
Ngày tải lên: 08/03/2014, 22:20
New techniques in plant biotechnology
... Experiments are described in which flower heads are dipped in an A tumefaciens suspension (‘floral dip’) resulting in the insertion of T-DNA in the germ line cells.7 In addition, in theory it cannot ... grafting on GM rootstock Grafting is a technique that has been used for centuries in plant breeding In grafting, the bud-bearing part (the graft) of a plant is grafted onto the rootbearing part ... of) a plant- based gene with the aim of integrating the mutation introduced into the gene construct into the plant- based gene In this way, a plant- based gene can also be target activated via insertion...
Ngày tải lên: 13/03/2014, 21:56
Psychological complications in sickle cell disease pptx
... ischaemic brain injury or stroke is reported to be the most disabling complication in SCD, with younger children generally developing infarcts while older patients are more inclined to haemorrhage ... dysfunction, include learning deficits in reading and mathematics, have also been identified (Armstrong et al, 1996) Lesion size in relation to intellectual functioning has been documented in children ... underlying CBT is that difficulties in living, relationships, general health, etc., have their origin in (and are maintained by) thoughts, emotions and behaviours The aim of cognitive interventions...
Ngày tải lên: 14/03/2014, 11:20
Báo cáo khoa học: Sugar signalling and antioxidant network connections in plant cells pptx
... metabolized Invertases (INVs) can in uence plant growth and development either directly (e.g by in uencing source–sink relationships or by acting as a true regulatory protein) or indirectly via ... unclear [43] Plants possess three types of invertase: acid cell wall invertases (glycoside hydrolase family 32) located in the apoplast (i.e the continuum of cell walls of adjacent plant cells), acid ... contributing to increasing antioxidant abilities [17] and assisting in recycling sugars from sugar radicals (Fig 1) Cellular NDP-sugar concentrations Sugar signalling and antioxidant networks in plants...
Ngày tải lên: 15/03/2014, 11:20
Báo cáo khoa học: New roles of flavoproteins in molecular cell biology: Blue-light active flavoproteins studied by electron paramagnetic resonance pptx
... hyperfine couplings from protons whose nuclear spins are interacting only very weakly with the unpaired electron spin, e.g protons from the protein backbone within the cofactor binding pocket, protons ... way during migration [22,23] A feature that distinguishes cryptochromes from photolyases is an extension of variable length and sequence that interacts with other proteins involved in intracellular ... [76]: the protein consists of an a ⁄ b-domain and a FAD-binding domain The binding pocket, which is smaller but deeper than those of CPD photolyases, is strictly hydrophobic and contains conserved...
Ngày tải lên: 16/03/2014, 02:20
Báo cáo khoa học: New roles of flavoproteins in molecular cell biology: Histone demethylase LSD1 and chromatin pot
... histone H3 tail polyamines polyamines aromatic amines aromatic amines aromatic amines L-amino acids glycine, sarcosine sarcosine gene expression regulation polyamines metabolism vitamin B5 biosynthesis ... green This analysis is in agreement with the domain organization in the first line The third line shows a summary of the identified linear motifs (after filtering processing taking in account the accessibility ... off in MAO A and B (Fig 3A) The SWIRM domain is a motif commonly found in chromatin enzymes and is responsible for protein–protein and DNA–protein interactions [36] The name of this domain derives...
Ngày tải lên: 16/03/2014, 02:20