k channels in human and mouse ventricular myocytes

... cells Figure Clustering of mouse IKDCs and human CD16 cells Clustering of mouse IKDCs and human CD16 cells Hierarchical clustering with complete linkage was performed on the indicated cell populations ... mouse and human leukocyte subsets Clustering of mouse and human leukocyte subsets Hierarchical clustering with complete linkage was performed on the indicated cell populations isolated from: (a) mouse, ... that mouse and human pDCs cluster together and apart from cDCs (Figure 3) Importantly, when analyzing the DC datasets alone, mouse CD8α and human BDCA3 cDCs on the one hand, and mouse CD11b and human...

Ngày tải lên: 14/08/2014, 08:20

... Holland R, Howe KL, Howe K, Johnson N, Jenkinson A, Kahari A, Keefe D, Kokocinski F, Kulesha E, Lawson D, Longden I, Megy K: Ensembl 2008 Nucleic Acids Res 2008:D707-714 12 Wang Z, Ding G, Yu Z, ... T, Batalov S, Lapp H, Ching KA, Block D, Zhang J, Soden R, Hayakawa M, Kreiman G, Cooke MP, Walker JR, Hogenesch JB: A gene atlas of the mouse and human protein-encoding transcriptomes Proc Natl ... with the intervening spacers containing less than 10 genes, since they were confused with tandem duplicates Finally, we preserved 410 and 680 pairs resulting from We identified 1,210 tandem duplicates...

Ngày tải lên: 14/08/2014, 13:21

... are indicated as error bars The experiments in (A) and (B) were performed in parallel replication origin (pJC389) and either recombinant human or murine DNA polymerase a-primase expressed using ... an intact origin of replication; either plasmids carrying the noncognate PyV origin or a disabled JCV origin are inactive (Fig 1A, column and Fig 1B, columns and 9) Background values determined ... replication of a plasmid carrying the JCV origin Columns and 3, SV40 Tag with human and murine DNA polymerase a-primase, respectively; columns and 5, JCV Tag with human and murine DNA polymerase a-primase,...

Ngày tải lên: 17/03/2014, 03:20

... isoform, which lacks a 17 amino acid insert and the KTS insert in the zinc finger region, did not influence GP210 expression in SAOS osterosarcoma cells, in a system using tetracycline-induced repression ... Sorokin L, Ocklind G, Mattson R, Ekblom P: Induction of mouse tenascin expression by a human Wilms tumor cell line growing in nude mice Int J Cancer 1993, 54:868-74 Ekblom M, Klein G, Fecker L, Mugrauer ... during mitosis and one single consensus Ser1880-Pro1881 motif could be detected as a target for cyklin-B-p34cdc2 kinase and MAP kinase in vitro [39] A comparison of the cytoplasmic domain in mouse, ...

Ngày tải lên: 11/08/2014, 08:20

... Alu in humans and B4 in mice have the highest ratio of insertions within introns (66%), whereas L1 and LTR both in human and mouse have the lowest percentage of copies within introns (58% in human ... human and 56% in mouse for L1, 44% in human and 52% in mouse for LTR) L1 and LTR exhibit a biased insertion in the antisense orientation relative to the mRNA within intronic sequences in both human ... exon in DMWD gene among mammals Exon sequences are marked in blue, flanking intronic sequences are marked in black, and the canonical AG and GT dinucleotides at the 3'ss and 5'ss are marked in...

Ngày tải lên: 14/08/2014, 07:21

... obtained 22,598 human genes and 24,064 mouse genes Of these, 8,312 in human and 8,105 in mouse are single-copy genes, and the remaining are clustered by Ensembl into 3,538 families in human and 3,600 ... 10-3 per gene per MY in human and 0.027 to 0.035 × 10-3 per gene per MY in mouse For the entire genome, assuming the same substitution rates, and 19,032 in human and 20,453 in mouse to be the effective ... the neutrality of Ks was questioned by later studies [4-7] Accordingly, Gu and coworkers [4] proposed that a combination of Ks and other neutral markers, such as intron and flanking regions, should...

Ngày tải lên: 14/08/2014, 08:20

... temperature of human inside-out erythrocyte vesicles in transport assay buffer resulted in decreased cGMP transport in incubations including mg AEBSFÆmL)1 but not in incubations lacking AEBSF (Fig ... containing 10 lg of protein were incubated with the indicated concentration of substrate in a final volume of 25 lL of vesicle buffer containing 10 mm MgCl2, 10 mm creatine phosphate and creatine kinase ... was routinely checked by Ponceau S staining of the nitrocellulose membrane After blocking for h in NaCl ⁄ Pi containing 1% nonfat dry milk, 1% BSA, and 0.05% Tween-20, the membrane was incubated...

Ngày tải lên: 07/03/2014, 11:20

... example, the human orthologous sequence of rodent mir-350 could be localized in an intron of the KARP-1-binding protein (KAB) gene However, a nine base-pair deletion in the human and chimpanzee ... located in known 64 M J Weber coding genes (see below) Many human miRNAs are located in introns of noncoding mRNAs In general in these cases, the mRNA was not or poorly conserved in the mouse genome ... genome When human miRNAs were in nonconserved genes, or outside characterized genes, I examined the known flanking genes In all cases but three, human and mouse miRNAs were found to reside in conserved...

Ngày tải lên: 07/03/2014, 16:20

... Thagard 1986 Induction: Processes of Inference, Learning, and Discovery Cambridge, MA: MIT Press Knoke, David, and James H Kuklinski 1982 Network Analysis Quantitative Applications in the Social ... agent is an input-output device that performs formally defined tasks following the "sense-think-act cycle." After gathering information (the input), the agent processes it by linking the input to ... analysis of the covariance of variables Network analysis (e.g., Knoke and Kuklinski 1982) offers techniques for describing and analyzing the connectedness of individuals, objects, or events It does...

Ngày tải lên: 14/03/2014, 21:20

... maintaining marketcompetitive pay, shortand longer-term incentives Internal movement and job shifts as organizational restructuring occurs Shakeout Time Shakeout Extensive internal training and ... whether to own final interviewing Receive interview training from HR unit Do final interviewing and hiring where appropriate Review reference information Provide feedback to HR unit on hiring/rejection ... • Recruiting and selecting for current openings • Conducting safety training • Resolving employee complaints Longer term (2–5 years) • Assessing workforce trends and issues • Engaging in community...

Ngày tải lên: 28/06/2014, 08:20

... After extensive washing in water, slides were counterstained with Papanicolaou (Merck AG, Dietikon, Switzerland) and mounted in Merckoglass (Merck AG, Dietikon, Switzerland) Staining specificity ... PDZ-binding motif, involved in cytoskeletal and signal transduction interactions [7] JAM-C was initially described as an adhesion molecule localized at interendothelial contacts and as an integrin ... expression of JAM-C in a pattern similar to that found in human arthritic tissues Expression of the protein was mainly found in the lining layer and was associated with vessels in the sublining layer RT-PCR...

Ngày tải lên: 09/08/2014, 10:20

... Classification and diagnostic prediction of cancers using gene expression profiling and artificial neural networks Nat Med 2001, 7:673-679 25 Lukk M, Kapushesky M, Nikkila J, Parkinson H, Goncalves ... clusters, human and mouse samples exhibit subclustering in proximity to each other In the nervous system cluster, a few mouse head and neck samples (yellow) are mixed in - these are retina samples ... system, cell lines, adipocyte tissues, immune system, skin and gastrointestinal organs, Page of 11 human and mouse data clustered side by side on both X and Y axis (Figure 3) Within such tissue...

Ngày tải lên: 09/08/2014, 22:23

... Characterization of hHK-1- and endokinins-induced responses in guinea pig airways Contractile effects of hHK-1 and endokinins in isolated guinea pig airways Human hemokinin-1 induced concentration-dependent ... 75(3):334-41 Kong ZQ, Yang WL, Tao Y, Shi XM, Fu CY, Zhao RF, Wang R: Effects of rat/ mouse hemokinin-1, human hemokinin-1 and human hemokinin-1(411), mammalian tachykinin peptides, on rate and perfusion ... blockage by tachykinin antagonists and the role of hemokinin in T lymphopoiesis Blood 2003, 102(6):2165-2172 16 Weinstock JV: The role of substance P, hemokinin and their receptor in governing...

Ngày tải lên: 12/08/2014, 11:22

... UK) in methanol (BDH Laboratory Supplies, Poole, UK) Sections were incubated in 2.5% horse blocking serum (Vectastain Universal Quick Kit, Vector Laboratories, Peterborough, UK) prior to Avidin ... with diaminobenzidine substrate (Vectastain Universal Quick Kit, Vector Laboratories, Peterborough, UK), counterstained in haematoxylin (BDH Laboratory Supplies, Poole, UK) Image capture and semi-quantitative ... Leicestershire, UK) containing mls of scintillation liquid (Amersham Biosciences) and counted by a β-counter; Beckmann Instruments (Beckman Coulter Ltd, High Wycombe, Buckinghamshire, UK) Cell count...

Ngày tải lên: 12/08/2014, 14:20

... tagging method for monitoring dynamic changes in gene expression by using an ultra highthroughput sequencer Biotechniques 2008, 45:95-97 Shiraki T, Kondo S, Katayama S, Waki K, Kasukawa T, Kawaji ... single-linkage clustering In Additional data file we compare the promoteromes obtained with our clustering procedure with those that were obtained with the simple single-linkage clustering procedures ... clustering procedures used in FANTOM3 The key difference between our clustering and the single-linkage clustering employed in FANTOM3 is that, in our procedure, neighboring TSSs with significantly...

Ngày tải lên: 14/08/2014, 21:20

... qualifications and may not understand or sometimes they not want to answer Accordingly prior to interviewing, investigating and interviewing cadres must be trained and a trial interview will be made in the ... 2.2.5 Indicators assessing the use in research: The infection ratio of dog roundworm larvae in human; in dogs, in surroundings (soil) 2.2.6 Use definitions and concepts in research: The infection ... washing hands before eating, using labor protection means like boots, gloves, etc when working and directly exposing to soil, deworming periodically for dog in order to restrict risk Researching...

Ngày tải lên: 29/10/2014, 22:17

... Ligand Ionic Strength Glycylglycine Glycylserine Alanylglycine Glycylalanine 0.01 0.01 0.01 0.01 Glycylglycylglycine Imidazole Proline Varied 0.03 Constant Mg2+ Ca2+ Mn2+ K1 K2 K1 K1 K1 K2 K1 K2 ... K1 K2 K1 K2 Glycine Cysteine Serine Arginine Aspartic acid Histidine Lysine Glutamic acid Glutamine Asparagine Varied Varied 0.15 0.15 0.15 0.01 0.10 0.10 0.01 0.01 0.01 Varied 0.16 0.01 β2 K1 ... mammals, including humans.59 TABLE 1.3 Factors Affecting Mineral Bioavailability Intrinsic Factors Animal species and its genetic makeup Age and sex Monogastric or ruminant (intestinal microflora)...

Ngày tải lên: 23/11/2015, 15:35

... Tokyo, Japan) containing 10% fetal bovine serum [8–12] cDNAs for human and mouse sPLA2s, human cyclooxygenase (COX)-2 and human microsomal prostaglandin E2 (PGE2) synthase (mPGES) and their HEK293 ... 31202 Suzuki, N., Ishizaki, J., Yokota, Y., Higashino, K. , Ono, T., Ikeda, M., Fujii, N., Kawamoto, K & Hanasaki, K (2000) Structures, enzymatic properties, and expression of novel human and mouse ... sequences of the C-terminal part of mouse sPLA2-IID (mIID) and its mutants, KE2 and KE2RS2 Two and four basic amino acids are replaced by glutamic acid or serine in KE2 and KE2RS2, respectively...

Ngày tải lên: 18/03/2014, 01:20

... Cathepsins K, L, and S in Human Breast, Lung, and Cervical Cancer Binbin Chen and Manu O Platt* Abstract Background: Cathepsins K, L, and S are cysteine proteases upregulated in cancer and proteolyze ... All human recombinant cathepsins were from Enzo Life Sciences (Plymouth Meeting, PA) Human cathepsins K and S were expressed in insect cells, human cathepsin V was expressed in NSO cells, and human ... of recombinant cathepsin K (0.2, 0.5, 1, and ng) and cathepsin L (45, 220, 450, and 900 ng) were loaded per gel for quantifying absolute quantities of cathepsins K and L B) Cathepsins K and L activity...

Ngày tải lên: 18/06/2014, 22:20