its role in adolescent development

... provide information concerning the kinetics and properties of the enzyme More strikingly, we show a novel role of the PPase enzyme in the development and molting process of A suum larvae in vitro ... Vmax (maximum velocity) values were determined by incubating the diluted recombinant proteins in the standard reaction mixture in the presence of increasing concentrations of PPi (0.05–0.5 mM) ... removed and minced with a surgical knife The minced tissue was wrapped in cotton gauze and suspended in NaCl/Pi containing 100 lgÆmL)1 penicillin/ streptomycin at 37 °C for h After incubation the...

Ngày tải lên: 20/02/2014, 11:20

... deletion of RasGEF; KIND1, KIND1 domain; KIND2, KIND2 domain (B) KIND2 domain anchors v-KIND to dendrites Flag-tagged v-KIND, DKIND1, DKIND2, DKIND1 + 2, DRasN, DGEF, KIND1 or KIND2 together with ... very-KIND-MAP2 interaction A 37–217 456–620 KIND1 KIND2 v-KIND ΔKIND1 ΔKIND2 ΔKIND1+2 ΔRasN ΔGEF KIND1 KIND2 ΔKIND1-Flag RasN ΔKIND2-Flag 1461–1734 1742 GEF ΔKIND1+2-Flag dendrite axon ΔGEF-Flag KIND1-Flag ... functional determinants of the protein–protein interaction between v-KIND and MAP2 (i.e the MAP2-binding core module in v-KIND and the v-KIND-binding core module in MAP2) across a range of amino acid...

Ngày tải lên: 14/02/2014, 19:20

... occludin in apoptosis and invasion A C B D E F Fig Identification of a human occludin splice variant in liver cell lines (A) Occludin protein expression was examined with an antibody to the N-terminus ... cells In Chang cells, stained occludin was observed in the cytoplasmic region Additionally, in OccWT-overexpressing Hep3B and Huh7 cells, regions stained with antibody to hemagglutinin (HA), indicating ... To determine the function of occludin, we reduced occludin expression in Huh7 cells by using occludin short hairpin RNA (shRNA) After determining the reduced expression level of occludin with...

Ngày tải lên: 18/02/2014, 18:20

... folding of the mutant The lower intensity may arise from partitioning of the protein into an aggregated state The effect of copper binding on the secondary structure was determined using CD in ... C-terminus (box in Fig 1) at sites: 92, 75, 66 and 58 As histidine residues are central to copper binding in many proteins they probably form part of the copper binding sites in this protein Although ... remains to be seen by more in vivo studies Stefin B as a copper binding protein We have demonstrated that human stefin B is a high affinity copper binding protein It exerts two high affinity biding...

Ngày tải lên: 19/02/2014, 06:20

... in the AUH gene This indicated that AUH, in addition to its RNA binding function, must play an important role in leucine catabolism AUH was overproduced in E coli and characterized by measuring ... suggesting that this enzyme had another function in cellular metabolism Indeed, AUH had enoyl-CoA hydratase activity, which was described as an additional intrinsic function of the protein Its role ... localisation of AUH, an AU-specific RNA-binding enoyl-CoA hydratase Gene 228, 85–91 Nakagawa J & Moroni C (1997) A 20-amino-acid autonomous RNA-binding domain contained in an enoyl-CoA hydratase Eur J...

Ngày tải lên: 19/02/2014, 07:20

... Cancer Institute, Shanghai, China Smith RK, Carroll PM, Allard JD & Simon MA (2000) MASK, a large ankyrin repeat and KH domain-containing protein involved in Drosophila receptor tyrosine kinase ... cultured in RPMI containing 10% FBS, 1% penicillin– streptomycin and interleukin-2 growth factor Monocytes were isolated using CD14 beads and differentiated into macrophages and dendritic cells using ... may play a role in promoting proliferation and preventing apoptosis [8] dMASK mediates receptor tyrosine kinase (RTK) signaling, independent of MAP kinase (MAPK) by either functioning downstream...

Ngày tải lên: 07/03/2014, 21:20

... mLámin)1 The following gradients were used: incubations containing arbutin or salicin as substrate; 100% C initial, increasing D to 20% (10 min), 50% (15 min), 100% (5 min), held at 100% (5 min); ... (genistin) Daidzein-7-Glc (daidzin) Daidzein-7-MalGlc Flavonols Quercetin-4¢-Glc (spiraeoside) Quercetin-7-Glc Quercetin-3,4¢-diGlc Flavones Apigenin-7-Glc (apigetrin) Luteolin-4¢-Glc Luteolin-7-Glc ... Naringenin-7-GlcRha (naringin) Hesperetin-7-GlcRha (hesperidin) Phloretin-7-Glc (phlorizin) Oleuropein Cyanidin-3-Glc (kuromanin) Cyanidin-3,5-diGlc Flavanones Dihydrochalcones Secoiridoids Anthocyanidins Ó FEBS...

Ngày tải lên: 08/03/2014, 16:20

... Engineering in providing services to the government, the public, and the scientific and engineering communities The Council is administered jointly by both Academies and the Institute of Medicine ... PHYSICS AND ITS ROLE IN SPACE EXPLORATION EXPLORERS Status Ongoing line of scientifically focused principal-investigator-led missions Mission The Explorer program has, since the beginning of the ... Printing Office, Washington, D.C., 2004 SOLAR AND SPACE PHYSICS AND ITS ROLE IN SPACE EXPLORATION the fundamental role of solar and space physics research both in scientific exploration and in...

Ngày tải lên: 14/03/2014, 10:20

... deficit IV THE ROLE OF INTERI T RATES IN THE ADJUSTHKUT PROG*MM Among the multitude of reasons arguing for interest rate reform in Egypt it is its role in facilitatingthe country's ongoing process ... discounted A Interest Rate and Business Investment The first step in examining how higher interestrates may influence business investmentdecisions and performances is the understandingof firms' ... publicat largeremainvery dim In that case, of banks will continueto operateas the dominantfinancial intermediary the in Egyptian financial system Figure 1, Deposit Banking Institutions: Total...

Ngày tải lên: 15/03/2014, 14:20

... production of a fusion protein bearing an N-terminal extension of 20 residues including a His6-tag At a later stage, a point mutation was introduced in the vector pET28a-TbPEX14-N in order to fuse the ... several copies in the N-terminal domain of all PEX5s [22,32,38] In this paper, in vitro experiments showed that also in T brucei the N-terminal part of TbPEX14 (amino acids 1–146) interacts specifically ... various molar ratios Protein concentrations were determined by the Bradford method using bovine serum albumin as standard [28] This method was also used in experiments involving subcellular fractionation...

Ngày tải lên: 23/03/2014, 17:21

... B7/CD28dependent and -independent induction of CD40 ligand expression J Immunol 1995, 155:5124-5132 Curtsinger JM, Schmidt CS, Mondino A, Lins DC, Kedl RM, Jenkins MK, Mescher MF: Inflammatory cytokines provide ... Wiekowski M, Lira SA, Gorman D, Kastelein DA, Sedgwick JD: Interleukin-23 rather than interleukin-12 is the critical cytokine for autoimmune inflammation of the brain Nature 2003, 421:744-748 Nakae ... References 5.* 10 11 12 13.* 14 15 16 Delon J, Stoll S, Germain RN: Imaging of T-cell interactions with antigen presenting cells in culture and in intact lymphoid tissue Immunol Rev 2002, 189:51-63 O’Sullivan...

Ngày tải lên: 09/08/2014, 01:23

... the intestinal epithelium of rats receiving radiotherapy Previous research into expression at the protein level has shown conflicting findings In one study, proinflammatory cytokine protein levels ... Pro-inflammatory cytokines were upregulated at later time points of radiotherapy suggesting that these cytokines can ultimately induce more tissue injury and inflammation in the intestine with increasing total ... cleared in xylene, coverslipped and viewed using light microscopy Staining intensity was graded according to a previously published and validated grading system where = no staining, = weak staining,...

Ngày tải lên: 09/08/2014, 08:22

... loss during intestinal tumorigenesis may, in part, act through upregulation of b-catenin, increasing intestinal b-catenin signaling and cell proliferation Also, increase in small-intestinal mucosa ... regulating cell cycle genes and interacting with key DNA damage-activated checkpoint proteins Per1 overexpression in cancer cells increases ionizing radiationinduced apoptosis, whereas inhibition ... repair is found to display prominent circadian oscillations in mouse brain reaching at its maximum in the afternoon/early evening hours and minimum in the night/early morning hours The circadian oscillation...

Ngày tải lên: 10/08/2014, 09:20

... cytokine owing to inherited defects might further contribute to the pathophysiology of asthma Optimal signaling by IL-4 (reviewed in [23]) involves its interaction with receptors consisting of ... of high-affinity IL-4Rα and either the common γ chain or the IL-13 receptor α chain Binding of IL-4 results in the tyrosine phosphorylation of signal transduction molecules including motifs similar ... Acting as a decoy to bind and neutralize circulating cytokine, coupled with the high specificity and high affinity of binding for the cytokine, makes the soluble receptor ideal as a cytokine...

Ngày tải lên: 12/08/2014, 18:20

... also increasingly involved in the development and delivery of a range of education and training programmes designed to help individuals working in HIV advance professionally, from administering ... best practice and advocacy in the fight against HIV/AIDS The IAS is also strengthening its commitment to regional development, expanding our involvement in three main areas: collaboration with ... Providing education and training opportunities for its membership is important for any professional society, and the IAS is looking at ways to strategically expand its role in this area in order...

Ngày tải lên: 13/08/2014, 09:20

... molecular chaperones in the folding of oxidized proteins Refolding of colloidal thyroglobulin by protein disulfide isomerase and immunoglobulin heavy chain-binding protein J Biol Chem 2001, 276:21337-21342 ... bacitracin, a PDI inhibitor Finally, PDI expression was induced by the antiinflammatory cytokine IL-10, and IL-10-mediated inhibition of LPS-induced IL-6 expression was reduced by bacitracin These findings ... cytokines play a major role in sepsis-induced liver injury [19,20], we also investigated the expression of PDI in cells of the macrophage-like cell line, RAW 264.7, after incubation with LPS In...

Ngày tải lên: 13/08/2014, 11:22

... Lorand 1989) Interaction with integrins makes it possible for TG2 to be involved in integrin-dependent signal transduction, including in the focal adhesion kinase (FAK) and GTP-binding proteins Raf-1 ... at its protease sensitive hinge region, which is between the N-terminal ECM binding domain and the C-terminal LAP binding domain This can be achieved by many proteinases in the serine proteinase ... Tyr583 are involved both in GDP and ATP binding to TG2 The residue Arg580, which is important for GTP/GDP binding, is not involved in ATP binding (Han et al 2010) By purifying the insulin-like growth...

Ngày tải lên: 24/08/2014, 10:58

... heme-binding regions in HRI: an N-terminal domain of HRI and in an insert region in the protein kinase domain of HRI (63) Heme, in the presence of iron, binds to α and β globin chains in ratio ... cytoplasmic splicing of mRNA encoding the Xbox binding protein1 (XBP1) in mammals and homologue of the mammalian activating transcription factor/cAMP-response element (CRE)-binding protein (HAC1) in yeast ... with ribosomes Heme binds to amino-terminal sequences of HRI, along with a kinase insert region, leading to inhibition of eIF2 kinase activity In response to iron deficiency in erythrocytes, heme...

Ngày tải lên: 24/08/2014, 12:32

Ngày tải lên: 14/11/2014, 06:30

... knockdown MIN6 cell line Figure4.2 Increased betagranin storage in BIG3-deficient MIN6 cells Figure4.3 Increased insulin storage in BIG3-deficient MIN6 cells ix Figure4.4 Increased betagranin secretion ... protein recruitment distiguishes the cargo-loaded subdomains from the rest of TGN Curvature-inducing proteins, such as the Bin/amphiphysin/Rvs (BAR) domain family and the GRIP-domain-containing ... binding of a cargo protein with a motor protein dynein (Tai et al., 1999); or mediated via adaptor proteins (Nakagawa et al., 2000), or binding to a lipid domain (Noda et al., 2001) Protein kinase...

Ngày tải lên: 11/09/2015, 09:15