isolation purification and identification of active
Isolation, screening and identification of virulent isolates of Bipolaris oryzae causing rice brown spot and Sarocladium oryzae causing sheath rot disease
... aim of the present study is the isolation and screening of Bipolaris spp and S oryzae and confirmation at species level by molecular technique Materials and Methods Collection of brown spot and ... show detail of the location The samples were brought to the laboratory for microscopic examination, isolation, purification and pathogenicity test Isolation of the pathogens Isolation of Bipolaris ... the production of conidia The morphology of mycelium and conidia was observed under light microscope The presence of septation in mycelia and conidia and the colour of the mycelia and conidia were...
Ngày tải lên: 30/05/2020, 20:50
Methods for the isolation, maintenance and identification of yeasts
... the isolation, maintenance and identification of yeasts D Yarrow Contents 1 Isolation of yeasts 77 2 Maintenance of yeast cultures 78 3 Procedures for the identification of yeasts 80 4 List of ... Add 40 g of glucose, 5 g of peptone, 20 g of agar, and 500 ml of yeast infusion to 500 ml of demineralized water and dissolve Alternatively, 5 g of powdered yeast extract in 500 ml of demineralized ... extract, 5g of glucose, 8g of des- iccated ox bile, 1 ml of glycerol, 0.5 g of glycerol monostearate, 0.5 ml of Tween 60, 10 ml of whole- fat cow's milk, and 12 g of agar in 1 liter of Trang 4demineralized...
Ngày tải lên: 04/06/2020, 11:42
Isolation, characterization and identification of putative bacterial endophytes from some plants in hot springs, South Dakota
... 100% 0 Typha Nasturtium officinale Nasturtium officinale Nasturtium officinale Nasturtium officinale Nasturtium officinale Nasturtium officinale Nasturtium officinale Acinetobacter calcoaceticus ... development of selected endophytic bacterial strains that can promote plant growth could be one of many new approaches that are needed to aid the growth and health of agricultural plants Isolation and ... roots of monocotyledons plants, Typha, Bromus tectorum, and Festuca The remaining eight strains were isolated from roots of dicotyledons plants, Nasturtium officinale For the isolation of endophytes,...
Ngày tải lên: 14/10/2020, 18:38
Isolation, selection and identification of aspergillus oryzae producing high salt tolerant neutral protease
... RESULTS AND DISCUSSION 25 4.1 Isolation and primary identification of Aspergillus oryzae 25 4.1.1 Isolation of Aspergillus oryzae from the natural sources 25 4.1.2 Primary identification of ... 21 3.2.3 Effect of pH on activity and stability of protease 23 Trang 53.2.4 Effect of NaCl concentrations on activity and stability of protease 23 3.2.5 Identification of Aspergillus oryzae ... rate of fungus TB1 on CYA and PDA after 2 days and 5 days 33 Figure 4.5 Effect of NaCl concentrations on protease activity of two isolates TB1 and G2 34 Figure 4.6 The NaCl tolerance of the...
Ngày tải lên: 10/03/2021, 16:03
Isolation and identification of soilborne fungi in fields irrigated by GAP in harran plain using two isolation methods
... 5000 and 4700 microfungus propagules in the samples of soils with a water holding capacity of 38% and 21.7% and 66,000 and 50,000 in samples of soils with a water-holding capacity of 48% and 44%, ... After the identification of these isolates they reported 100 discrete species and strains and 15 sterile microfungi They found approximately 46,326 propagules of microfungi in 1 g of oven-dried ... 1993), the vicinity of ‹zmir (Ekmekçi, 1974 a and b; 1975; Asan & Ekmekçi, 1994; Öner, 1974) and Thrace (European Turkey) (Asan, 1997 a and b). Isolation and Identification of Soilborne Fungi...
Ngày tải lên: 09/01/2020, 18:41
Isolation and identification of Rhizopus oligosporus local isolate derived from several inoculum sources
... and Laboratory of Pest and disease of Plant, Faculty of Agriculture of Udayana University Sampling of Herbicus leaves, Tectona leaves, various brands and the origin of tempeh and various brands ... Herbicus leaves, Tectona leaves, various brands and origin of tempe and various brands and origin of tempeh starter in several provinces of Bali Maintenance of isolates through regeneration every ... inoculum consisting of 18 samples from waru leaves, 18 samples from teak leaves, 18 samples from various brands and the origin of tempe and 18 samples from various brands and origin of tempeh starter...
Ngày tải lên: 11/03/2020, 11:08
Isolation and identification of indole acetic acid producing bacteria from the coasts of Ben Tre and Tra Vinh provinces
... of Genome Research, VAST, Vietnam 2 Graduate of Science and Technology, VAST, Vietnam 3 Institute of Chemistry and Materials, Academy of Military Science and Technology, Vietnam 4 Institute of ... Nguyen Huy Hoang, 2019 Isolation and identification of indole acetic acid producing bacteria from the coasts of Ben Tre and Tra Vinh Provinces Tap chi Sinh hoc (Journal of Biology), 41(4): 55–67 ... The aim of this study was to investigate the IAA producing bacteria from the coastal regions of Ben Tre and Tra Vinh Provinces, Vietnam Trang 3MATERIALS AND METHODS Isolation and screening of IAA...
Ngày tải lên: 26/03/2020, 01:52
Mining and identification of polyunsaturated fatty acid synthesis genes active during camelina seed development using 454 pyrosequencing
... acid) and 52 % linoleic acid (LA) (omega-6 fatty acid) [1] The optimal dietary fatty acid profile includes a low intake of both saturated and omega-6 fatty acids and a moderate intake of omega-3 ... sequences and vector sequences were filtered out Finally, 521,507 and 310,125 clean reads were obtained from 10 DAF and 20 DAF with average lengths of 630 bp and 654 bp Furthermore, 25,398 and 23,678 ... levels of 15.61 % of unigenes increased more than 2-fold in 20 DAF and 9.64 % of genes increased more than 2-fold in 10 DAF (Fig 4B) The differences in the expression of shared genes were of interest...
Ngày tải lên: 26/05/2020, 21:12
Enumeration, isolation and identification of diazotrophic bacteria in rhizosphere soil of two rice varieties in Jammu district, India
... Enumeration, Isolation and Identification of Diazotrophic Bacteria in Rhizosphere Soil of Two Rice Varieties in Jammu District, India Harkirat Singh and Renu Gupta * Division of Soil Science and Agricultural ... Basmati-370 and Ratna variety in rhizosphere soil of rice grown in different areas of Jammu district using nitrifying medium (Lewis and Pramer, 1958) Microscopic examination and identification of gram ... performed on bacterial isolates of diazotrophic bacteria obtained from rhizosphere soil of Basmati-370 and Ratna rice growing areas of RS Pura and Bishnah blocks of Jammu district showed positive...
Ngày tải lên: 29/05/2020, 10:54
Isolation and identification of Bacillus species from soil for phosphate, potassium solubilisation and amylase production
... Aleksandrov agar medium After 10 days of incubation, the difference between zone of clearance and colony diameter was found to be 9 mm and that of Sample code 9 was found to be 5 mm Out of ten, ... Collection of samples Ten soil samples were collected from various fields, gardens of Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut and Bafawat village of Meerut district ... (Sample code 3 and 9) out of ten Among these two isolates, Sample code 3 showed highest potassium solubilisation and the clear zone on Aleksandrov agar medium was 9 mm and that of Sample Code...
Ngày tải lên: 06/08/2020, 01:33
Isolation and identification of heterorhabditis bacteriophora from tea plantation of Assam
... of head (b) Presence or absence of epiptygma (c) Shape and size of spicules (d) Shape and size of gubernaculum (e) Presence or absence of post anal swelling in adult females (f) Tail shapes of ... recovery of 0.5% EPN distribution depends on temperature, precipitation and soil type and is closely related to vegetation type and presence of insect host (Nielsen and Philipsen, 2003 and Puza and ... differentiation of species Morphological characters of 20 specimens each of infective juveniles, males and females of first and second generation were observed Morphological observations and quantitative...
Ngày tải lên: 20/09/2020, 19:23
Isolation, purification and structural characteristics of glycosaminoglycans from sea cucumber stichopus horrens
... pattern of sulfation of N-Acetyl-Galactosamine and fucose residues IR and NMR spectra of two frations showed that sulfate groups were primarily occupied at C4 of pyranose residues in F2 and C6, C2 and/or ... (FS) was built of the repeating α-L-fucopyranosyl residues 2 MATERIALS AND METHODS 2.1 Isolation and purification of glycosaminoglycans from sea cucumber S horrens * Isolation of glycosaminoglycans: ... frequency of 500 MHz at a temperature of 70 oC 3 RESULTS AND DISCUSSION 3.1 Isolation and purification Figure 1 Fractionation of glycosaminoglycans from body wall of sea cucumber S horrens by anion...
Ngày tải lên: 28/06/2021, 15:16
isolation and identification of mirnas in jatropha curcas
... pGEMT vector and sequenced A total of 426 and 356 sequences were collected from leaf and seed libraries respectively Analysis of these se-quences resulted in identification of 233 and 114 unique ... the near fu-ture The identification of miRNA targets is essential for understanding miRNA functions Identification of miRNAs and their targets is challenging, which was often done computationally ... testing of gene function because it is time consuming and laborious [21] In this study, we isolated a collection of miRNAs and their target genes in jatropha, and examined the expression patterns of...
Ngày tải lên: 04/12/2022, 15:13
isolation and identification of two novel attractant compounds from chinese cockroach eupolyphaga sinensis walker by combination of hsccc nmr and cd techniques
... simultaneous isolation and purification of two attractants from Eupolyphaga sinensis Walker which are candidate pheromones The attractants’ planar structures were identified by 1D and 2D NMR and MS ... solid-phase microextraction [14] Although a variety of chromatographic systems have been used to separate and analyze attractants, isolation and purification of insect attractants are still a tough task ... www.mdpi.com/journal/molecules Article Isolation and Identification of Two Novel Attractant Compounds from Chinese Cockroach (Eupolyphaga sinensis Walker) by Combination of HSCCC, NMR and CD Techniques Wei...
Ngày tải lên: 04/12/2022, 15:14
Isolation and identification of agrobacterium rhizogenes inducing hairy roots of catharanthus roseus in vivo
... trên môi trường Yeast Extract Mannitol Agar (YEMA), phát triển được trên môi trường kiềm pH 11 Hofer‟s agar và khả năng tổng hợp 3-ketolactose theo Murugesan et al [12] Ngoài ra, các chủng vi ... chuyển hóa lactose thành 3-ketolactose (màu vàng là dương tính); F: phát triển trên trên môi trường Hofer‟s pH 11; G: phát triển và bắt màu của sinh khối với thuốc thử Congo trên môi trường YEMA; H: ... khả năng bắt màu đỏ với thuốc thử Congo trên môi trường YEMA, phát triển được trên môi trường Hofer‟s có pH 11 Ngoài ra, khả năng chuyển hóa lactose thành 3-ketolactose cũng đã được Murugesan...
Ngày tải lên: 18/02/2023, 08:05
Isolation and identification of agar degrading microorganisms from diverse environments in vietnam (khóa luận tốt nghiệp)
... Institute Isolation, purification and maintenance of strains Grouping of strains by morphology and protein fingerprinting Identification of strains by DNA sequencing Determination of agarase ... "Isolation and identification of agar-degrading microorganisms from diverse environments in Vietnam" was conducted to Trang 12understand the diversity of the group of microorganisms, and ... Research methods (Fu and Kim, 2010) 17 3.3.1 Sample enrichment 17 3.3.2 Method of isolation 17 Trang 63.3.3 Purification and maintenance of isolates 17 3.3.4 Observation of colonies and cells 17...
Ngày tải lên: 11/07/2023, 21:12
Báo cáo khoa học: "Isolation and identification of Escherichia coli O157:H7 using different detection methods and molecular determination by multiplex PCR and RAPD" docx
... Switzerland) Once suspicious colonies are identified, confirmation of the isolates as E coli O157:H7 is dependent upon biochemical identification and demonstration of the presence of somatic and ... strains), C1-6 (strains of Cornell Univ.) and P1-7 (strains from E coli reference center of Pennsylvania State Univ.) b The presence of Stx1 and Stx2 “-” and “+” indicate negative and positive, respectively ... assessment of the risks difficult, and the options for management and control unclear The objectives of this study are (i) to examine the prevalence of E coli O157:H7 in slaughterhouses and retail...
Ngày tải lên: 07/08/2014, 18:21
Báo cáo y học: "Isolation and identification of bioactive compounds in Andrographis paniculata (Chuanxinlian)" potx
... activity of the nhexane and methanol extracts of A paniculata Seven compounds, namely andrographolide, bis-andrographolide 14-deoxy-11,12-didehydroandrographolide, andrograpanin, 14-deoxyandrographolide, ... compounds including andrographolide enhance proliferation and IL-2 secretion in hPBL [41] Andrographolide enhances secretion of IL-2 and IFNγ by T cells and stimulates the production of cytotoxic T ... terms of bioactive properties and abundance Among the andrographolide analogues, 14-deoxy-11,12-didehydroandrographolide is immunostimulatory, anti-infective and anti-atherosclerotic; neoandrographolide...
Ngày tải lên: 13/08/2014, 15:21
Isolation and identification of components from ixeris sonchifolia hance as potential anti stroke agents
... Representative HPLC profiles for purified compounds to and and fractions to 74 3.4.1 Structures of sesquiterpene lactones 75 3.4.2 Skeletal structures of flavonoids, isoflavonoids and neoflavonoids 76 ... Discussion and conclusions Chapter Isolation and characterization of components from ethyl acetate fraction of Ixeris sonchifolia Hance 48 55 3.1 Introduction 56 3.2 Materials and methods 56 3.2.1 Isolation ... observe, feel and understand the world I’m writing this thesis to share with all of them I Table of contents Acknowledgements I Table of contents II Abstract VII List of tables X List of figures...
Ngày tải lên: 11/09/2015, 10:06
isolation, selection and identification bacillus subtilis from muddreg of beer
... aquaculture and especially in environmental treatment It helps disintegrate dreg, mud In this study Isolation selection and identification of Bacillus subtilis from “mud-dreg of beer” 21 strains of bacteria ... 16S-9F and 16S-1525R The result of PCR perform size band of strains and Bacillus subtilis control had 1500bp Sequencing perform that strains (Bs4 and BN5) were Bacillus subtilis (99% and 98% ... waste water of beer and beer wallows in the brewing process Objective: Isolation, selection and identification of Bacillus subtilis from beer dregs, that is done with the aim of isolating, identifying...
Ngày tải lên: 06/10/2015, 12:58