isolation purification and culturing of omf

... pattern of sulfation of N-Acetyl-Galactosamine and fucose residues IR and NMR spectra of two frations showed that sulfate groups were primarily occupied at C4 of pyranose residues in F2 and C6, C2 and/or ... (FS) was built of the repeating α-L-fucopyranosyl residues 2 MATERIALS AND METHODS 2.1 Isolation and purification of glycosaminoglycans from sea cucumber S horrens * Isolation of glycosaminoglycans: ... frequency of 500 MHz at a temperature of 70 oC 3 RESULTS AND DISCUSSION 3.1 Isolation and purification Figure 1 Fractionation of glycosaminoglycans from body wall of sea cucumber S horrens by anion

Ngày tải lên: 28/06/2021, 15:16

... Sharma et al., (2013), Chand and Chandra (2014), Deep et al., (2014), Taware et al., (2014) and Koley and Mahapatra (2015) Effect of culture media on growth and sporulation of Alternaria brassicae ... specimens in lacto-phenol cotton blue and examined under compound microscope for presence of microorganism if any Isolation of causal organism Small bits of desired size of infected samples were cut ... suggested by Gomez and Gomez (1986) The standard error and critical difference were worked out and the results obtained were compared statistically Results and Discussion Examination of diseased samples

Ngày tải lên: 09/01/2020, 17:13

... niger and A flavus The diameter of the zone of inhibition was shown in Table 4 The purified fucoidan could inhibit the growth of A niger and A flavus The MIC value and the zone of inhibition of ... buffers of Tris-HCl, phosphate, acetate and four given NaCl concentrations The effects of buffers and concentrations of NaCl were depicted in Figure 1 Figure 1 The effects of buffers (a) and concentrations ... R B., Padalia R B., and Shinde K D - Extraction and purification of C-phycocyanin from dry Spirulina powder and evaluating its antioxidant, anticoagulation and prevention of DNA damage activity,

Ngày tải lên: 13/01/2020, 06:31

... https://doi.org/10.20546/ijcmas.2019.805.120 Isolation, Production and Characterization of the Polysaccharide “Xanthan Gum” from Xanthomonas spp B.M Rana and A.A Raval* Department of Microbiology, Arts, Science and Commerce ... broth in SC1 and SC4 was 2.80 g/cm3 and 2.74 g/cm3 respectively And residual sugar in SC1 and SC4 was 0.243 mg/ml and 0.228 respectively In our study xanthan was extracted at 120 hrs and xanthan ... (Table 4 and 5) Sucrose SC1 and SC4 were inoculated in sucrose (2%) containing production medium and viscosity Trang 5of the broth was measured Sucrose was used as a source of carbon and residual

Ngày tải lên: 13/01/2020, 23:19

... brought out the role and efficiency of fungi in solubilizing insoluble phosphate, zinc, and hydrolysis of cellulose, production of phytohormones (IAA and GA3) which may help in growth of the plant when ... fungal isolates and the results obtained were as follows Results and Discussion Isolation and In vitro screening of phosphate solubilizing fungi (PSF) The rhizosphere soil samples of soybean were ... isolates were examined for the production of IAA and GA3. The amount of IAA and GA3 produced by the seven isolates was determined (at 13 and 10 DAI for IAA and GA3 respectively) The results are

Ngày tải lên: 14/01/2020, 09:31

... oil and jojoba oil etc The present study was conducted to demonstrate the isolation, identification and screening of biosurfactant producing bacteria and production of biosurfactant Materials and ... 48, 72 and 96h of incubation The emulsification index was generated as E0, E24, E48, E72, and E96 (EI) at 0, 24, 48, 72 and 96 h to check the stability of the emulsion formed (Anandraj and Thivakaran, ... A.S and Costa, S.F 2008 Investigation of an outbreak of Enterobacter cloacae in a Trang 10neonatal unit and review of the literature The Journal of Hospital infection 70(1): 7-14 Desai, J.D and

Ngày tải lên: 14/01/2020, 16:29

... ng of DNA, 1X PCR buffer, 10 mM of dNTPs mixture, 10 pmol of each primer and unit of Taq DNA polymerase Materials and Methods The present study was carried out to isolate efficient isolate of ... minutes and final shock at 4°C A 20 µl PCR reaction mixtures that consisting 50 ng of DNA, 1X PCR buffer, 10 mM of dNTPs mixture, 10 pmol of each primer and unit of Taq DNA polymerase Collection and ... microcentrifuge tubes and incubated at 16oC for 16 h for ligation Preparation of competent cells Transformation of E coli DH5α Results and Discussion Isolation and identification of Pseudomonas fluorescens

Ngày tải lên: 15/01/2020, 08:39

... temperature optima of 40°C and retaining an activity of 76% at a temperature range of 20-80°C and followed normal Michael-Menten curve with the kinetic parameter K m and V max of 0.5455mM and 33.927µmol/min ... (Fig 4) A summary of the purification scheme is given in Table4 A 23 fold purification of the phytase was achieved with a recovery of 47.8% and enzyme exhibited an activity of about 66.5U/mg ... optimize and understand the interaction between 4 selected variables i.e galactose, ammonium sulphate, beef extract and yeast extract in an experiments of 30 runs The coded levels of variable and

Ngày tải lên: 11/03/2020, 11:58

... production and effect on paddy seed germination and seedling growth Materials and Methods Collection of leaf samples The leaf samples of paddy were collected from different agro climatic conditions of ... ml of the Salkowski reagent was added Incubated at room temperature for 25 minutes and then read at OD530 Using the standard curve for IAA, the amount of IAA was calculated Effect of isolates of ... (Baki and Anderson, 1973) After taking the dry weight of shoot and root (g), Root Shoot ratio was calculated using the formula, Root Shoot Ratio = Dry weight of root (g) × 100 Dry weight of

Ngày tải lên: 21/05/2020, 21:09

... aim of the present study is the isolation and screening of Bipolaris spp and S oryzae and confirmation at species level by molecular technique Materials and Methods Collection of brown spot and ... show detail of the location The samples were brought to the laboratory for microscopic examination, isolation, purification and pathogenicity test Isolation of the pathogens Isolation of Bipolaris ... morphology of mycelium and conidia was observed under light microscope The presence of septation in mycelia and conidia and the colour of the mycelia and conidia were observed Identification of S oryzae

Ngày tải lên: 30/05/2020, 20:50

... Add 40 g of glucose, 5 g of peptone, 20 g of agar, and 500 ml of yeast infusion to 500 ml of demineralized water and dissolve Alternatively, 5 g of powdered yeast extract in 500 ml of demineralized ... extract, 5g of glucose, 8g of des- iccated ox bile, 1 ml of glycerol, 0.5 g of glycerol monostearate, 0.5 ml of Tween 60, 10 ml of whole- fat cow's milk, and 12 g of agar in 1 liter of Trang 4demineralized ... the isolation, maintenance and identification of yeasts D Yarrow Contents 1 Isolation of yeasts 77 2 Maintenance of yeast cultures 78 3 Procedures for the identification of yeasts 80 4 List of

Ngày tải lên: 04/06/2020, 11:42

... 100% 0 Typha Nasturtium officinale Nasturtium officinale Nasturtium officinale Nasturtium officinale Nasturtium officinale Nasturtium officinale Nasturtium officinale Acinetobacter calcoaceticus ... development of selected endophytic bacterial strains that can promote plant growth could be one of many new approaches that are needed to aid the growth and health of agricultural plants Isolation and ... roots of monocotyledons plants, Typha, Bromus tectorum, and Festuca The remaining eight strains were isolated from roots of dicotyledons plants, Nasturtium officinale For the isolation of endophytes,

Ngày tải lên: 14/10/2020, 18:38

... N., and Panda, T 2003 Purification and biochemical properties of microbial pectinases Process Biochem., 38: 987-996 Hammed, I M S A., and Alyaa, M 2010 Purification and characterization of ... polygalacturonic acid and pectin as substrates Determination of energy of activation and The energy of activation (Ea) was estimated from the slope of Arrhenius plot obtained by plotting the Log of initial ... of distilled water Fill the 3 ml syringe with the sample and slowly charge the focus chamber Then, 6 ml of 0.1 M H3PO4 and 6 ml of 0.1 M NaOH were respectively added to the ventilation hole of

Ngày tải lên: 05/11/2020, 04:17

... Pfaller MA, Houston A, Coffmann S Application of CHROMagar Candida for rapid screening of clinical specimens for Candida albicans, Candida tropicalis, Candida krusei and Candida (Torulopsis) glabrata ... tissue, blood, and urine promotes growth of Candida The present study is in keeping with studies done by Kandhari et al., with an incidence of 20.4% and Shroff with an incidence of 14.66% The ... predominance of Candida tropicalis (30%) and Candida parapsilosis (30%) being the most common species followed by Candida albicans (20%) and Candida krusei (20%) The incidence of blood stream

Ngày tải lên: 05/11/2020, 04:56

... 21 3.2.3 Effect of pH on activity and stability of protease 23 Trang 53.2.4 Effect of NaCl concentrations on activity and stability of protease 23 3.2.5 Identification of Aspergillus oryzae ... RESULTS AND DISCUSSION 25 4.1 Isolation and primary identification of Aspergillus oryzae 25 4.1.1 Isolation of Aspergillus oryzae from the natural sources 25 4.1.2 Primary identification of ... rate of fungus TB1 on CYA and PDA after 2 days and 5 days 33 Figure 4.5 Effect of NaCl concentrations on protease activity of two isolates TB1 and G2 34 Figure 4.6 The NaCl tolerance of the

Ngày tải lên: 10/03/2021, 16:03

... sativa L.: Isolation, Characterization, and Anchal Tripathy 1 and Chandi C Rath 2* 1 Department of Botany, College of Basic Science and Humanities, Odisha University of Agriculture and Technology ... Optimization of culture conditions of fermentation Optimization of physical parameters Effect of incubation period It was observed that the isolates OSLST-4and GA374.365µg/ml and59.490µg/ml on 8th and ... maximum amount of GA3 (78.656µg/ml) under submerged fermentation in a medium containing (3%) of NaCl, (1%) of starch and (0.3%) of ammonium chloride at pH-6, on 240 hrs (10th days) of incubation

Ngày tải lên: 28/04/2021, 01:46

... sativa L.: Isolation, Characterization, and Anchal Tripathy 1 and Chandi C Rath 2* 1 Department of Botany, College of Basic Science and Humanities, Odisha University of Agriculture and Technology ... Optimization of culture conditions of fermentation Optimization of physical parameters Effect of incubation period It was observed that the isolates OSLST-4and GA374.365µg/ml and59.490µg/ml on 8th and ... maximum amount of GA3 (78.656µg/ml) under submerged fermentation in a medium containing (3%) of NaCl, (1%) of starch and (0.3%) of ammonium chloride at pH-6, on 240 hrs (10th days) of incubation

Ngày tải lên: 28/06/2021, 16:42

... ethidium bromide and UV irradiation for detection of DNA in gels, thereby preventing DNA damage of the long molecules and enhancing isolation of full-length clones The range of cloning kits and vectors ... ordering process and the cost of synthesis is relatively cheap An example of appropriate design of primers to add attB1 and B2 sites to the 5′ and 3′ ends respectively of a gene of interest are ... N-glycosidic bonds between uracil and deoxyribose leading to a loss of the base The loss of bases leads to disruption of base pairing and therefore exposure of single strands that can be annealed to...

Ngày tải lên: 25/10/2013, 22:20

... thankful to Professor Les Copeland (Faculty of Agriculture, Food and Natural Resources - The University of Sydney, New South Wales, Australia) and Professor Kaeko Kamei (Department of Biomolecular ... proteins Generally, the two-step chromatography of AEX and HIC was sufficient for the purification of FIII-1, FIII-2, and FII, since their SEC and SDS-PAGE profiles represented pure proteins Fraction ... alignment of fragments obtained from MS/MS analyses of FIII-1 and FIII-2 from P excavatus with lumbrokinase and its precursor from L rubellus (sp:P83298 and U25647) and E fetida (gpu:EU167737 and AY438624),...

Ngày tải lên: 20/06/2014, 23:20

... hexakisphosphate) is the primary storage form of phosphorus in plants They occupy about 1-3% of the seeds of grains and legumes, and 60-80% of the total amount of plant phosphorus (Nelson, 1967) Phosphorus ... PuriWcation and structural characterization Protein Expression and Purification 46, pp.221–232 Bradford, M.M 1976 A rapid and sensitive method for quantitation of microgram quantities of protein ... determination of inorganic orthophosphate and it’s application to the assay of inorganic pyrophosphate Anal Biochem 113:313–317 Kim, Y O., Kim, H K., Bae, K S., Yu, J H and Oh, T K 1998 Purification and...

Ngày tải lên: 06/10/2015, 13:05