isolation identification and characterization of butanol tolerant bacteria

... Trang 1ISOLATION, IDENTIFICATION AND CHARACTERIZATION OF BACTERIAL ANTAGONISTS OF THE DRAGON FRUIT FUNGAL PATHOGEN Neoscytalidium dimidiatum ... activity of isolated bacteria on the mycelial growth after 2 days (A) and 5 days (B) of incubation on PDA medium The inhibitory percentage (C) and inhibitory zone after 5 days and 10 days of incubation ... germ tubes (B) The growth of mycelia in the present of bacterial cultured supernatants on PDA Bacterial isolates suppressed growth of N dimidiatum mycelia The effect of bacterial isolates on the

Ngày tải lên: 25/10/2022, 11:32

... entitled Isolation and Identification of Avian Pathogens Library of Congress Catalog Card Number: 2008924452 Trang 5A Laboratory Manual for the Isolation, Identification and Characterizationof Avian ... a manual of procedures for isolation and identification of pathogens to a more encompassing manual for diagnosis of the disease and isolation and/or demonstration of the pathogen This change ... need for standardizing testing of vaccines had been met, the scope and purpose of the manual was broadened to be a resource for laboratory procedures for the isolation and identification of disease-causing

Ngày tải lên: 24/04/2023, 08:14

... https://doi.org/10.20546/ijcmas.2017.605.246Isolation and Characterization of Phyllospheric Methylotrophic Bacteria of Paddy from Middle Gujarat, India Ronak R Prajapati*, R.V Vyas and Y.K Jhala Department of Agricultural ... cfu of PPFMs per leaflet and typically >80% of the viable bacteria recovered from leaves were PPFMs (Omer et al., 2004) Materials and Methods Isolation of phyllospheric methylotrophic bacteria ... detection of methanol dehydrogenase (MDH) was carried out following method of Eggeling and Sahm (1980) with some modifications Results and Discussion Isolation of phyllospheric methylotrophic bacteria

Ngày tải lên: 27/09/2020, 13:22

... important role of endocytic regulatory factors in internalization and cell physiology Identification and Characterization of Endocytic Proteins The following body of work aims to identify and characterize ... membrane, made up of lipids and proteins, which prevented diffusion of complex molecules into and out of the cell As these organisms evolved, and eukaryotes diverged from other early bacteria, they ... fields of biochemistry and genetics continued to contribute to our understanding of how proteins and lipids are sorted and trafficked between myriad intracellular locales, advances in electron and

Ngày tải lên: 14/11/2014, 06:20

... DEGREE OF DOCTOR OF PHILISOPHY INSTITUTE OF MOLECULAR AND CELL BIOLOGY NATIONAL UNIVERSITY OF SINGAPORE 2008 Trang 2I would also like to thank Jian Liang from Walter’s lab; and Guo Ke, Li Jie and ... mouse and fugu; and identification of CNEs………… 62 Trang 54.3 Expression pattern of mouse Six3………67 4.4 Functional assay of Six3 CNEs………70 4.4.1 Basal promoter region (includes CNE13) of mouse ... sequence of the human genome, and to identify and characterize all functional elements which would lead to a more complete understanding of the structure, function and evolutionary history of the

Ngày tải lên: 11/09/2015, 16:02

... love and support and their unwavering faith in my abilities Trang 4Table of Contents Acknowledgments………2 Table of contents……… 4 Summary……… 6 List of tables……….7 List of figures………8 List of abbreviations……… ... cis-regulatory elements through comparisons of promoters and 5` flanking regions of γ-globin and ε-globin gene of several primates and other mammals [58] Thus alignment of orthologous nucleotide sequences ... Institute of Singapore (GIS) for his help and his unlimited patience in getting the bioinformatics part of the project off the ground His cheerfulness and his willingness to go out of the way

Ngày tải lên: 14/09/2015, 08:40

... in Iceland and Japan and highest in USA and France The overall prevalence was the lowest in Northern Ireland, UK and Finland, and the highest in Italy, Spain and Martinique The burden of SLE ... kindness, and help Thanks to all staff of Deparment of Rheumatology and Immunology, Singapore General Hospital for their help and friendship Trang 4Thanks to all staff of Department of Clinical ... SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF MEDICINE YONG LOO LIN SCHOOL OF MEDICINE NATIONAL UNIVERSITY OF SINGAPORE 2007 Trang 3ACKNOWLEDGEMENT First of all, I would like

Ngày tải lên: 14/09/2015, 12:42

... 21 and Der f 5 116 5.2.4 Secondary structures of Der f 21 and Der f 5 1185.2.5 IgE-binding of Der f 21 and Der f 5 in consecutive atopic 132 Chapter 6: Identification and Characterization of ... 144 Figure 6.4 C and D Correlation of IgE antibodies between Sui m 5.01 and 5.02 (C) and between Sui m 5.01 and Sui m 21 (D) 145 Figure 6.5 SDS-PAGE profile of a panel of Group 5 and Group 21 proteins ... Effect of Temperature, pH and Chemical Denaturant on Blo t 5 and Blo t 21 IgE-binding Trang 11Chapter 5: Identification and Characterization of Der f 21: a Der f 5 Homologue 105 5.2.1 Identification

Ngày tải lên: 14/09/2015, 12:44

... Preparation of C albicans and S cerevisiae genomic DNA 35 2.5.3 Preparation of C albicans and S cerevisiae RNA 36 2.6.4 Constructs in the study of CaMNN5 38 2.8 Indirect immunofluorescence staining of ... Assay of CaMnn5p mannosyltransferase activity 45 CHAPTER 3 Isolation and functional characterization of a novel Candida albicans gene CaMNN5 that suppresses the dependent growth defect of Saccharomyces ... the O-linked mannans Deletion of both copies of MNT1 and MNT2 resulted in decrease in the level of in vitro mannosyltransferase activity and truncation of O-mannan, and the double mutant was attenuated

Ngày tải lên: 16/09/2015, 08:30

... topology of claudins Fig 9: Creating a paracellular seal Fig.10: Proposed model of ion and size discriminations and flux of charged and charged molecules non-Fig 11: Interaction of two of the major ... structure and expression of claudin-16 Fig 42: A schematic model of magnesium resorption in the cortical ascending limb (cTAL) of Loop of Henle Fig 43: Predicted topology of CLDN16 and the location of ... transport of charged and uncharged solutes should occur with different kinetics (Anderson et al., 2004) Fig 10: Proposed model of ion and size discriminations and flux of charged and non-charged

Ngày tải lên: 16/09/2015, 08:31

... Trang 1IDENTIFICATION AND CHARACTERIZATION OF Trang 2IDENTIFICATION AND CHARACTERIZATION OF AGROBACTERIUM TUMEFACIENS VirD2-BINDING PROTEINS ... Preparation of recombinant proteins 67 2.4.2 Isolation of VirD2-binding proteins 68 2.5 In-gel Digestion, MALDI-TOF Analysis and Tandem MS Sequencing 69 2.6 Expression of VBP Proteins and Generation ... Assay 79 Chapter 3 Identification and Characterization of a VirD2-binding Protein 80 3.1 Identification of a Novel VirD2-binding Protein 80 3.2 Expression and Purification of Three VBP Proteins

Ngày tải lên: 16/09/2015, 15:55

... analysis and presentation of data 58 3.1 Isolation of p35-associated proteins and identification of protein kinase CK2 as an inhibitor of neuronal Cdk5 kinase 61 3.1.2.1 Isolation of p35-binding ... for sharing his materials and helpful discussions and Wong Boon Seng for helpful advices and critical comments of my manuscript All the past and present members of IMCB and our laboratory for making ... subunit of CK2 On the other hand, others have reported that CK2 activity and its Trang 20al., 1990) Using immunofluorescence and immunoelectron microscopy, Yu and coworkers showed that CK2α and CK2β

Ngày tải lên: 16/09/2015, 17:10

... little work has been done in the characterization of a glioblastoma specific promoter tumour-This study deals with the identification, isolation and characterization of a glioblastoma specific promoter ... kidney, adrenal gland and spleen It was also shown that HSV-Tk caused depletion of the enteric glia and this resulted in severe inflammation and hemorrhagic necrosis of the jejunum and ileum (Bush ... promoter is that of the survivin gene Survivin is a member of the Inhibitor of Apoptosis (IAP) protein family and plays a vital role in the survival of cancer cells and the progression of malignancy

Ngày tải lên: 22/10/2015, 21:20

... University of Singapore for supporting me with a Postgraduate Research Scholarship Trang 3List of poster presentation 1 Identification and characterization of endogenous CD157-ligand in mammalian ... Conference: Receptor-Ligand Interactions (Molecular, Physiological and Pharmacological aspects), May 2002, Bergen, Norway 2 Identification and characterization of interacting protein of CD157 7th NUH ... Electrophoresis of PCR product of CD157 fragment without Figure 1.3 Electrophoresis of PCR product of Fc fragment on 1% CD157-Fc 39 Figure 2.2 Electrophoresis of PCR product of CD157 and CD157-GPI

Ngày tải lên: 22/10/2015, 21:20

... Trang 1IDENTIFICATION AND CHARACTERIZATION OF SHORT VEGETATIVE PHASE (SVP) TARGET GENES WU YANG (B Sc.) A THESIS SUBMITTED FOR THE DEGREE OF MASTER OF SCIENCE DEPARTMENT OF BIOLOGICAL ... successfully evolved and predominant group of land plants, characterized by their most remarkable feature, i.e flowers They represent the most widespread group of land plants and one of the only two ... and Sussex, 1992; Steeves and Sussex, 1989) Underneath the central zone and in the deep layers of the meristem lies the rib zone, which forms the pith of SAM and gives rise to the most part of

Ngày tải lên: 22/10/2015, 21:20

... levels of FSH and LH Figure 1: Plasma levels of FSH and LH in coho salmon during reproductive maturation (modified from Dickhoff and Swanson, 1990) The expression and pulsatile secretion of these ... 1979, Ng and Idler reported the presence of two types of gonadotropin in plaice and flounder pituitaries Subsequently, two different forms of GtH, GtH-I and GtH-II, were isolated from salmon and shown ... The studies of the amino acid (Itoh et al., 1988) and the cDNA sequences (Sekine et al., 1989) revealed close similarity of GtH-I and GtH-II to the mammalian FSH and LH, respectively, and thus

Ngày tải lên: 08/11/2015, 17:15

... review, the utility of CODEHOP-mediated PCR for the identification of novel viruses and the characterization of new viral genes and genomic regions is presented While the focus of this study was ... genomes Often the linear order of genes within the genomes of related viruses is maintained Thus, the spacing and orientation of specific genes can be predicted in the genomes of Page 15 of 24 ... phylogenies and tracing of evolutionary pathways Furthermore, comparison of multiple representatives of homologous viral proteins can be of importance for understanding the protein structure and function...

Ngày tải lên: 18/06/2014, 22:20

... mM EDTA, 1.5 M NaCl), in the presence of 0.1 mg of proteinase K and mg of lysozyme and incubated at 37°C for 30 Lysis was completed by adding ml of 20% SDS and incubated for h at 65°C with shaking ... species of bacteria including several unculturable bacteria in addition to the culturable proteolytic bacteria that are predominant and are involved in the degradation of the skin (Kayalvizhi and ... 65 and 85% of the maximum activity at the temperature range of 35°C and 55°C respectively Thermal stability of the purified AS-protease was estimated at Figure Multiple sequence alignment of...

Ngày tải lên: 21/06/2014, 05:20

... ‘chemical genetic’ approach of identifying and studying the mechanism of action of small-molecule agonists (and antagonists), we hoped to uncover some of the complexities of the Hh-signaling system ... activation with a Hh-protein ligand has therapeutic value [49,50] On the basis of our current understanding of these models and the specific mechanism of action of the Hh agonists, we predict ... binding of the Hh agonist to Smoexpressing cells This supports the model that all of these small-molecule modulators of Hh signaling are direct ligands of Smo We next asked whether a derivative of...

Ngày tải lên: 06/08/2014, 18:20

... amino-acid sequence of CaNDR1a (Figure 4), with residues S189 and G190 being strong ω-site candidates (with P-values of 2.48 × 10 -6 and 2.76 × 10 -5 , respectively) Furthermore, CaNDR1a and its Arabidopsis ... constitute a novel report on the identification and characterization of a functional NDR1 homolog, despite the plethora of orthologous candidates In this study, several lines of evidence indeed demonstrated ... Maluf M, Fernandez D: Identification of coffee WRKY transcription factor genes and expression profiling in resistance responses to pathogens Tree Genet Genomes 2010, 6:767-781 19 Fernandez D, Santos...

Ngày tải lên: 11/08/2014, 11:21