isolation characterization and differentiation of human embryonic stem cells
Activation of wntβ catenin signalling via GSK3 inhibitors direct differentiation of human adipose stem cells into functional hepatocytes
... is required to specify definitive endoderm from human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) Manipulations of Wnt signalling via glycogen synthase kinase ... we compared the effect of Wnt3a and two GSK3 inhibitors, Chir98014 and Chir99021 on the activation of Wnt/β-catenin signalling pathway, and the regulation of expression of definitive endoderm ... alternative sources of human hepatocytes are urgently required Recent studies have demonstrated that hepatocytes derived from human adipose stem cells (hASCs) are potentially scalable and applicable
Ngày tải lên: 19/11/2022, 11:45
influence of aging on the quantity and quality of human cardiac stem cells
... potential of bone marrow stem cells was impaired by renal failure and anemia9 Human cardiac stem cells also showed that chronic heart failure negatively affected the function of cardiac stem cells10,27 ... al Isolation and expansion of adult cardiac stem cells from human and murine heart Circ Res 95, 911–921 (2004). 3 Smith, R R et al Regenerative potential of cardiosphere-derived cells expanded ... tissue-specific stem cells and decreases their regenerative ability We therefore examined whether aging affected the quantity and quality of cardiac stem cells using cells obtained from 26 patients of various
Ngày tải lên: 04/12/2022, 14:53
isolation and expansion of human pluripotent stem cell derived hepatic progenitor cells by growth factor defined serum free culture conditions
... plating the cells, as previously described [6,7] Cultures of primary human fetal hepatocytes, human normal fetal liver-CD34+ cells, and HepaRG® cells which are terminally differentiated hepatic cells ... NCAM, CD13, and EpCAM U0126 decreased the expressions of FOXA2, CK19, and CD133 and increased those of AFP and ALB Since Y27632 increased the cell viability, combined effect of Y27632 and SB431542 ... Hayakawa, M.K Furue, H Mizuguchi, Efficient generation of functional hepatocytes from human embryonic stem cells and induced pluripotent stem cells by HNF4alpha transduction, Mol Ther 20 (2012)
Ngày tải lên: 04/12/2022, 15:13
Establishment of autologous culture systems for human embryonic stem cells
... 1ESTABLISHMENT OF AUTOLOGOUS CULTURE SYSTEMS FOR HUMAN EMBRYONIC STEM CELLS FU XIN (B.Sci (Hons.), NUS) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF ORAL AND MAXILLOFACIAL ... umbilical cord stem cells, adult stem cells and induced pluripotent stem (iPS) cells (Ariff Bongso, 2005, see Table 2) Each type of stem cells holds its own specific properties and advantages ... cell types of human body Therefore, study of the cellular and molecular properties of hESCs provides information in the culture, differentiation and applications of hESCs A review of the current
Ngày tải lên: 11/09/2015, 10:00
OCT 3 4 and SOX 2 are key factors for SDIA neurogenesis of mouse embryonic stem cells
... post-transfection and five days of selection were sufficient to achieve complete elimination of GFP-C2 negative control cells Mouse embryonic stem cells transfected with pcDNA6/TR and surviving Blasticidin ... you to Prof Too Heng-Phon for providing countless days of humor and inspiration, and providing invaluable thoughts on science and life, and all things in between Trang 3b Expression of Oct-3/4, ... 3 Knockdown of Oct-3/4 and Sox-2 Transcription Factors in Mouse Embryonic Stem Cells a Establishment of Efficient Transfection Method b Oct-3/4, Sox-2 Knockdown and Differentiation Into Trophectoderm
Ngày tải lên: 27/11/2015, 11:25
DSpace at VNU: Optimization of culture medium for the isolation and propagation of human breast cancer cells from primary tumour biopsies
... combination of various kinds of cells, including normal epithelial cells, stro-mal cells, breast cancer cells, and breast cancer stem cells A suitable protocol for the isolation of BCCs must ... Optimization of culture medium for the isolation and propagation of human breast cancer cells from primary tumour biopsies 1Laboratory of Stem Cell Research and Application, University of Science, ... develop a standardized protocol for the isolation and propaga-tion of HBC cells from primary tumour biopsies that a) ensures that the isolated primary cells include breast cancer stem cells and b)
Ngày tải lên: 16/12/2017, 03:41
Isolation, characterization and evaluation of pink pigmented facultative methylotrophs (PPFMS) associated with paddy
... that inoculation of PPFM bacteria could increase plant IAA concentrations and promote plant growth The present programme envisages isolation, characterization and evaluation of Pink Pigmented ... production and effect on paddy seed germination and seedling growth Materials and Methods Collection of leaf samples The leaf samples of paddy were collected from different agro climatic conditions of ... ml of the Salkowski reagent was added Incubated at room temperature for 25 minutes and then read at OD530 Using the standard curve for IAA, the amount of IAA was calculated Effect of isolates of
Ngày tải lên: 21/05/2020, 21:09
The effect of a germline mutation in the APC gene on β-catenin in human embryonic stem cells
... study of the earliest molecular mechanisms underlying malignant transformation in humans Human embryonic stem cells (hESCs) have already been proven to be a valuable tool for studying human genetic ... was also amplified and sequenced (Fig 2b) Characterization of FAP hESC lines as pluripotent stem cells The two FAP hESC lines (FAP1 and FAP2) were propa-gated for a long period of time (>50 passages) ... injection of the cells into immunodeficient mice Hematoxylin and eosin staining of paraffin-embedded sections of FAP1 teratoma developed in mice revealed different structures of differen-tiated cells
Ngày tải lên: 20/09/2020, 18:11
Isolation, characterization and identification of putative bacterial endophytes from some plants in hot springs, South Dakota
... 100% 0 Typha Nasturtium officinale Nasturtium officinale Nasturtium officinale Nasturtium officinale Nasturtium officinale Nasturtium officinale Nasturtium officinale Acinetobacter calcoaceticus ... development of selected endophytic bacterial strains that can promote plant growth could be one of many new approaches that are needed to aid the growth and health of agricultural plants Isolation and ... roots of monocotyledons plants, Typha, Bromus tectorum, and Festuca The remaining eight strains were isolated from roots of dicotyledons plants, Nasturtium officinale For the isolation of endophytes,
Ngày tải lên: 14/10/2020, 18:38
Báo cáo khoa học: "Human embryonic stem cells and therapeutic cloning"
... Gokhale PJ, Andrews PW Culture and characterization of human embryonic stem cells Stem Cells Dev 2004, 13, 325-336 18 Drukker M, Benvenisty N The immunogenicity of human embryonic stem-derived cells ... CP, Rao MS Enrichment of neurons and neural precursors from human embryonic stem cells Exp Neurol 2001, 172, 383-397 9 Chen U, Kosco M Differentiation of mouse embryonic stem cells in vitro; III ... leukemia, burns and spinal-cord injury Both adult and ES cells can be used for stem cell therapy In this review, we describe the derivation and characterization of ES cells and cloned ES cells Furthermore,
Ngày tải lên: 26/04/2021, 10:21
Endophytic fungi from Oryza sativa L.: Isolation, characterization, and production of GA3 in submerged fermentation
... sativa L.: Isolation, Characterization, and Anchal Tripathy 1 and Chandi C Rath 2* 1 Department of Botany, College of Basic Science and Humanities, Odisha University of Agriculture and Technology ... removing dust and debris and were then allowed to air dry Before surface sterilization the cleaned stems and leaves were cut into 0.5×0.5 cm²size.Isolation and Surface sterilization of endophytic ... maximum amount of GA3 (78.656µg/ml) under submerged fermentation in a medium containing (3%) of NaCl, (1%) of starch and (0.3%) of ammonium chloride at pH-6, on 240 hrs (10th days) of incubation
Ngày tải lên: 28/04/2021, 01:46
báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc
... expression pattern of undifferentiated and differentiated human embryonic stem cells Stem Cells Dev 2007, 16(6):1003-1016 Page 15 of 17 (page number not for citation purposes) Journal of Translational ... in adult cells (Figure 6, panel C) The differences in expression of these miRNA expression between adult cells and hES cells and between adult cells and EB were significant (P < 0.05) Of note, ... either EB or adult cells (Figure 6, panel A) The difference in the expression of miR-200c, miR-302b, and miR-367 between hES cells and EB, and between hES cells and adult cells was significant...
Ngày tải lên: 18/06/2014, 15:20
Directed differentiation of human embryonic stem cells into haematopoietic and definitive endodermal lineages
... endoderm differentiation of hESCs Table 5.1 Available information on knock-out phenotypes in the mouse vii LIST OF FIGURES Figure 1.1 Embryonic origin of human embryonic stem cells and their ... Mu ° – Degree x CHAPTER 1: GENERAL INTRODUCTION 1.1 Embryonic stem cells The isolation of mouse and human embryonic stem cells (ES cells) heralded a new era in regenerative medicine, raising hopes ... and Fraser 2005) However, unhindered analysis of developmental events was made possible with the isolation of embryonic stem cells Extensive investigation of the properties and capabilities of...
Ngày tải lên: 04/10/2015, 16:03
Báo cáo khoa học: Recruitment of transcription complexes to the b-globin locus control region and transcription of hypersensitive site 3 prior to erythroid differentiation of murine embryonic stem cells docx
... Transcription of LCR hypersensitive site in undifferentiated murine embryonic stem cells and in human CD 133+ bone marrow cells (A) Transcription of LCR regions and the bmaj-globin gene during differentiation ... nature and extent of covalent histone modifications and association of transcription complexes at the murine b-globin locus during the in vitro differentiation of murine embryonic stem cells We ... hematopoi- Fig Sequential activation of globin gene transcription during in vitro erythroid differentiation of murine embryonic stem cells PCR analysis of DNase I treated and reverse-transcribed total...
Ngày tải lên: 07/03/2014, 12:20
Báo cáo y học: "Hypertrophy is induced during the in vitro chondrogenic differentiation of human mesenchymal stem cells by bone morphogenetic protein-2 and bone morphogenetic protein-4 gene transfer" pps
... chondrogenic differentiation of mesenchymal progenitor cells and to up regulate the levels of type II collagen and aggrecan in chondrocytes and chondroprogenitor cells [1,7-11] During development of the ... Chondrogenic differentiation of cultured human mesenchymal stem cells from marrow Tissue Eng 1998, 4:415-428 35 Mueller MB, Tuan RS: Functional characterization of hypertrophy in chondrogenesis of human ... of primary MSCs and to investigate whether levels and extent of hypertrophy in vitro is influenced by the choice of transgene Materials and methods Construction and preparation of recombinant...
Ngày tải lên: 09/08/2014, 14:22
Fibronectin and laminin promote differentiation of human mesenchymal stem cells into insulin producing cells through activating Akt and ERK pptx
... phosphorylation status of Akt and ERK for stage III cells There was a baseline of Akt and ERK phosphorylation without adding ECM FN and LAM increased phosphorylation of Akt and ERK, and LAM had greater ... expression and survival of adherent cells [17] FN and LAM constitute the major ECM of pancreas islet cells Laminin-1 has been reported to promote the differentiation of fetal mouse pancreatic b -cells ... Itskovitz-Eldor J: Differentiation of human embryonic stem cells into insulin-producing clusters Stem Cells 2004, 22(3):265-274 Chang CF, Hsu KH, Chiou SH, Ho LL, Fu YS, Hung SC: Fibronectin and pellet...
Ngày tải lên: 10/08/2014, 05:21
báo cáo khoa học: "Epigenomics of human embryonic stem cells and induced pluripotent stem cells: insights into pluripotency and implications for disease" potx
... pluripotent embryonic stem cells and differentiation Nat Rev Mol Cell Biol 2006, 7:540-546 51 Park SH, Kook MC, Kim EY, Park S, Lim JH: Ultrastructure of human embryonic stem cells and spontaneous and ... embryonic stem cell; hESC, human embryonic stem cell; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of lysine 27 of ... epigenomic profiling of a cohort of human embryonic stem cell (hESC) lines can be used to generate hESC reference epigenomes (left panels) The extent of reprogramming and differentiation potential of individual...
Ngày tải lên: 11/08/2014, 12:21
The derivation, propagation, storage and gene expression of human embryonic stem cells on human feeders
... classes of pluripotent stem cells in humans, other primates and mice These are embryonic stem cells, embryonic germ cells, embryonic carcinoma cells and recently the discovery of a fourth class of ... Adult stem cells Adult stem cells also known as somatic stem cells can be found in diverse tissues and organs The most common adult stem cells are the hematopoietic stem cells, mesechymal stem cells ... blood cells and platelets, while skin stem cells give rise to the various types of skin cells Some recent reports suggest that adult stem cells such as haemopoietic stem cells, neuronal stem cells...
Ngày tải lên: 16/09/2015, 15:55
Transcriptome study of human embryonic stem cells and knockdown study of a pluripotency marker, LIN28
... Introduction 1.1 Human embryonic stem cells 1.1.1 Overview and characteristics of human embryonic stem cells Embryonic stem (ES) cells are isolated from the inner cell mass (ICM) of embryos of blastocyst ... List of Figures VIII List of Abbreviations IX Introduction 1.1 1.2 Human embryonic stem cells 1.1.1 Overview and characteristics of human embryonic stem cells 1.1.2 Regulatory networks and transcription ... HES3 Human Embryonic Stem Cells Undifferentiated HES4 Human Embryonic Stem Cells SAGE _Embryonic_ stem_ cell_H9_normal_p38_CL_SHES1 LSAGE _Embryonic_ stem_ cell_BG01_normal_p20_CL_SHE19 LSAGE _Embryonic_ stem_ cell_H13_normal_p22_CL_SHE15...
Ngày tải lên: 16/10/2015, 11:58
Engineered poly(l lactic acid) based nanofibers for osteogenic differentiation of human mesenchymal stem cells
... images of 2D (A and B), the outer surface of 3D (C and D) and the center of 3D (E and F) electrospun PLLA/Col nanofibrous scaffolds cultured with human MSCs in the Ost medium on day 28 Figures C and ... PLLA and TCPS In Figure b, OCN expression of human MSCs on the PLLA nanofibers was considerably lower than on the TCPS plates In Figures c and d, the expression levels of BSP and WNT5A of the cells ... nanofibrous bundles and (12) reservoir 183 Figure 6.2 The macroscopic images of 2D (A) and 3D (B); and the SEM images of 2D (C) and 3D (D) at 20000X magnification of electrospun PLLA/Col nanofibrous...
Ngày tải lên: 09/09/2015, 10:07