isolation and identification of mesenchymal stem cells

... field of view was per-formed to enable identification of GFP-positive cells and immunopositivity for matrix proteins in the same cells Results Identification of injected mesenchymal stem cells ... viability/metabolic activity of injected mesenchymal stem cells Photomicrographs representative of caspase 3 immunopositivity and polyA-mRNA staining in mesenchymal stem cells injected into tissue ... characteristics of injected mesenchymal stem cells following 4 weeks in culture Phenotypic characteristics of injected mesenchymal stem cells following 4 weeks in culture Photomicrographs representative of...

Ngày tải lên: 09/08/2014, 01:22

... 1CHARACTERIZATION OF THE SECRETION OF MESENCHYMAL STEM CELLS AND ITS RELEVANCE TO CARDIOPROTECTION LAI RUENN CHAI NATIONAL UNIVERSITY OF SINGAPORE 2011 Trang 2   CHARACTERIZATION OF THE SECRETION OF MESENCHYMAL ... interventions28 Mesenchymal Stem Cells In The Treatment Of Acute Myocardial Infarction With the emergence of stem cells as potential regenerative medicine, attempts to use stem cells to reduce ... 2004 Mesenchymal stem cells and their potential as cardiac therapeutics Circ Res 95, 9–20 Quesenberry, P.J., Aliotta, J.M., 2008 The paradoxical dynamism of marrow stem cells: considerations of stem...

Ngày tải lên: 10/09/2015, 08:42

... possible to derive and expand autologous stem cells from ovarian tissue The isolation and characterization of human PSCs will contribute considerably to the prospect of using stem cells to produce ... week Of these, 4.65% of the cells were positive for the germ cell marker Vasa and some of the cells were also positive for additional germ and stem cell markers, such as Fragilis, Thy-1, SSEA4 and ... populations of PSCs were observed: one with a cell diameter of with an increasing percentage of cells positive for germ and stem cell markers (Fig 2Dc) About 2.8% of all mouse testicular cells are...

Ngày tải lên: 16/12/2017, 04:38

... differentiation and/or producing a high level of desired lineages from the stem cells Thus, intense efforts have been dedicated to the identification of physical contributors in the regulation of stem cell ... environment of BMMSCs and found that the morphology and adhesion of BMMSCs were highly dependent on mechanical stiffness Cells became more spread and more adhesive on substrates of higher stiffness ... expression of surface markers through flow cytometric analysis and immunoflu-orescence assays The multipotency of the BMMSCs differentiated into mesenchymal lineages, including adipocytes and osteoblasts,...

Ngày tải lên: 15/01/2020, 00:46

... 1051 International Journal of Medical Sciences 2018; 15(10): 1051-1061 doi: 10.7150/ijms.25760 Research Paper Migration of mesenchymal stem cells to tumor xenograft models and in vitro drug delivery ... activity of CAL62/Rluc (R2=0.911), MDA-MB-231/Rluc (R2=0.934) cells and Fluc activity of MSC/Fluc (R2=0.91) cells increased with increasing cell numbers, as seen by BLI eGFP expression of MSC/Fluc ... or cancerous tissues, and visualized after sacrificing the animal MSCs are able to deliver drugs to target cells, and are an ideal candidate for cancer therapy The purpose of this study was to...

Ngày tải lên: 15/01/2020, 09:12

... initiation, growth, and invasion of cancer are driven by a small population of cancer stem cells (CSC) Previous studies have identified CD44+ cells as cancer stem cells in head and neck squamous ... initiating cells in HNSCC We confirmed the stemness feature of CD24+/CD44+ cells by showing that CD24+/CD44+ cells express higher levels of BMI1 and Nanog genes compared to CD24-/ CD44+ cells BMI1 ... because of the development of metastases and therapy-resistant local and regional recurrences [1] Progress in treatment and prognosis for HNSCC has been limited and the molecular mechanisms of HNSCC...

Ngày tải lên: 05/11/2020, 01:35

... be seen in the Table 1.1 Table 1.1 Polymers used for nanofibers to support stem cells 1.1.4 Mesenchymal stem cells Mesenchymal stem cells, first described by Friedenstein et al, they exist ... formation of the ECM (2) The beginning of angiogenesis The main cells that proliferate and develop at this stage are fibroblasts and endothelial cells Therefore, a large amount of growth factors and ... nanofibers and investigating the application of nanofibers for skin regeneration First, the gelatin nanofibers were fabricated by using the electrospinning method Then, the morphology and size of nanofibers...

Ngày tải lên: 22/12/2020, 22:04

... be seen in the Table 1.1 Table 1.1 Polymers used for nanofibers to support stem cells 1.1.4 Mesenchymal stem cells Mesenchymal stem cells, first described by Friedenstein et al, they exist ... formation of the ECM (2) The beginning of angiogenesis The main cells that proliferate and develop at this stage are fibroblasts and endothelial cells Therefore, a large amount of growth factors and ... nanofibers and investigating the application of nanofibers for skin regeneration First, the gelatin nanofibers were fabricated by using the electrospinning method Then, the morphology and size of nanofibers...

Ngày tải lên: 06/02/2021, 19:38

... Preconstruction of a vascular network through coculture of osteogenic cells (osteoblasts or mesenchymal stem cells (MSCs)) and vasculogenic cells (endothelial progenitor cells or endothelial cells (ECs)) ... of FSS to cultured cells Cells were incubated in a rocking culture system (DLAB, China) and subjected to FSS cycles We used a fixed rocking angle of 7o, frequency of 60 rpm, and fluid depth of ... effects of FSS and coculture with HU-VECs on osteogenic markers of BMSCs To further investigate the effects of 2 h/day FSS stimulation on osteogenesis of monoculture and cocultured cells, Cells...

Ngày tải lên: 12/01/2022, 22:32

... Hara et al., “Isolation, characterization, and in vitro and in vivo differentiation of putative thecal stem cells,” Proceedings of the National Academy of Sciences of the United States of America, ... reflected the same—mesodermal—origin of cells In spite of that, the gene expression profile of AFCs, BM-MSCs, and HDFs was different and indicated three distinct groups of cells There were eight genes ... broader gene expression profile of AFCs and elucidate the potential relation of AFCs to mesenchymal stem cells (MSCs), the most common cells tested in the regeneration of impaired ovarian function...

Ngày tải lên: 02/11/2022, 10:44

... initiation, growth, and invasion of cancer are driven by a small population of cancer stem cells (CSC) Previous studies have identified CD44+ cells as cancer stem cells in head and neck squamous ... initiating cells in HNSCC We confirmed the stemness feature of CD24+/CD44+ cells by showing that CD24+/CD44+ cells express higher levels of BMI1 and Nanog genes compared to CD24-/ CD44+ cells BMI1 ... because of the development of metastases and therapy-resistant local and regional recurrences [1] Progress in treatment and prognosis for HNSCC has been limited and the molecular mechanisms of HNSCC...

Ngày tải lên: 02/11/2022, 11:41

... As compared with embryonic stems cells and bone marrow-derived mesenchymal stem cells, adipose-derived mesenchymal stem cells (ADMSCs) have the distinct advantages of being abundant, easy to ... heart failure due to DCM [13,21] Isolation of Adipose-Derived Mesenchymal Stem Cells from Rat The 20 rats in groups 3 and 5 were anesthetized with inhalational isoflurane on day 7 prior to DCM ... expressions of surface markers of mesenchymal stem cell (CD27, CD29, CD45 and CD90) were remark-ably higher following 14 days of culturing Body Weight, Heart Weight, Lung Weight, and Serial Echocardiographic...

Ngày tải lên: 18/06/2014, 16:20

... the level of cells that home to tumors or other sites The tumor homing behavior of MSCs was demonstrated by the mere presence of these cells in the sites of interest and/or lack of such cells in ... unmodified cells Given complexity of the processes of biodistribution and homing of injected cells, we reasoned that an effect of engineered cell targeting would be more pronounced and better ... detected by imaging of whole animal (C) imaging of excised lungs (B) and by luciferase expression analysis of lung lysates (D,E) Data are presented as number of cells per mg of protein in lung...

Ngày tải lên: 20/06/2014, 07:20

... Switzerland) Once suspicious colonies are identified, confirmation of the isolates as E coli O157:H7 is dependent upon biochemical identification and demonstration of the presence of somatic and ... strains), C1-6 (strains of Cornell Univ.) and P1-7 (strains from E coli reference center of Pennsylvania State Univ.) b The presence of Stx1 and Stx2 “-” and “+” indicate negative and positive, respectively ... O157:H7 Rapid kit For the isolation of E coli O157:H7, conventional culture, immunomagnetic separation, and E coli O157:H7 Rapid kit were applied, and multiplex PCR and randomly amplified polymorphic...

Ngày tải lên: 07/08/2014, 18:21

... a role in the mainte-nance of the pluripotency of human embryonic stem cells [40] The maintenance of pluripotency of embryonic stem cells could involve Wnt signaling and could occur through a ... marrow mesenchymal stem cells (three normal, two osteoarthritis and one rheumatoid arthritis) and syn-ovium mesenchymal stem cells (two rheumatoid arthritis, two osteoar-thritis and two healthy) ... significantly induced the expres-sion of class II molecules, and both the number of positive cells (56 ± 16% and 36 ± 17% for BM-derived and synovium-derived cells, respectively) and their mean fluorescence...

Ngày tải lên: 09/08/2014, 07:20

... enchymal stem cells and neural progenitor cells. Haematolog- ica 2003, 88:126-133. 69. Reyes M, Verfaillie CM: Characterization of multipotent adult progenitor cells, a subpopulation of mesenchymal stem ... Growth factor receptor/haemopoietic stem and progenitor cells, mast cells [69] CD133 AC 133, hematopoietic stem cell Function unknown/haematopoietic stem cells and precursors antigen CD144 Cadherin-5 ... liver and adult bone marrow mesenchymal stem cells. Haematolog- ica 2005, 90:1017-1026. 20. Le Blanc K, Tammik L, Sundberg B, Haynesworth SE, Ringden O: Mesenchymal stem cells inhibit and stimulate...

Ngày tải lên: 09/08/2014, 08:23

... differentiation of mesenchymal stem cells (MSCs) Time-course expression of chemokines and their receptors during chondrogenic differentiation of mesenchymal stem cells (MSCs) The gene-expression profile of ... Modulation of the expression of cell-surface proteins on mesenchymal stem cells (MSCs) Modulation of the expression of cell-surface proteins on mesenchymal stem cells (MSCs) (a) and (b) Change ... (MSCs) Expression levels of ADAM and adhesion molecules during chondrogenic differentiation of mesenchymal stem cells (MSCs) The gene-expression profile of mesenchymal stem cells (MSCs; n = 2) was...

Ngày tải lên: 09/08/2014, 10:20

... ratio of MSCs to cancer cells, nature of tumour cells and cancer stem cells, integrity of immune system, number of stem cell pas-sages and site of injection; all can affect the outcome of MSCs ... dis-ease of dysfunction of the stem cells [32] Stem cells and tumor cells share similar signaling pathways that regulate self-renewal and differentiation, including the Wnt, Notch, Shh and BMP ... of stem cells and for understanding their behaviour in physiological and pathological conditions Niches are local tissue microenvironments that maintain and regulate stem cells [52], Livraghi and...

Ngày tải lên: 10/08/2014, 10:21

... inflammation and apoptosis Curcumin is a phytochemical capable of inhibiting IL-1β-induced activation of NF-κB and expression of apoptotic and pro-inflammatory genes in chondrocytes Therefore, the aim of ... num-ber of cells exhibiting typical morphological features of apoptotic cell death was determined by scoring 100 cells from 30 different microscopic fields per culture and the number of apoptotic cells ... Characterisation of canine adipose tissue derived mesenchymal stem cells In order to demonstrate that the cells isolated from the canine adipose tissue (Figure 1A) are indeed mesenchy-mal stem cells (MSCs)...

Ngày tải lên: 12/08/2014, 14:22

... infusion and within 15 minutes, 30 minutes, Mesenchymal stem cells used during this study Figure 1 Mesenchymal stem cells used during this study Typical morphological aspects of mesenchymal stem cells ... distribution of mesenchymal stem cells in vivo Figure 2 Early dynamic distribution of mesenchymal stem cells in vivo Sequential whole body scintigraphies after infu-sion of indium-111 labeled mesenchymal ... perspective of cellular therapy by intravenous stem cells infusion The participa-tion of stem cells in several vascular diseases pathogenesis was first proved with haematopoietic stem cells (HSCs)...

Ngày tải lên: 12/08/2014, 18:20