in vivo performance of cervical spine cages

... spectrum of the porphyrin contained in Zn-PP-KatA, in a pyridine⁄ water ⁄ NaOH⁄ Tween-80 mixture, was not similar to that of the reference solution of Zn-PP in the same solvent, indicating that ... protein, in binding porphyrins It is not known how haem is transported intracellularly in bacteria, and the mechanisms by which haem is incorporated into soluble and membrane-bound pro-teins inside ... compounds being taken up into the cell and incorporated into vital haem proteins instead of haem [5] We show here that haem analogues are indeed taken up and can be incorporated into haem proteins,...

Ngày tải lên: 06/03/2014, 11:20

... Chemical Engineering and 2School of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai, India Cellular regulation is a result of complex interactions arising from ... sequences of the GAL genes through its N-terminal DNA binding site Gal80p inhibits the transcriptional activity of Gal4p by binding to its 28 amino acid region at the carboxyl terminal [25–27] In vitro ... reflected by the sequence of interactions; (c) parameters quantifying strength of interactions; and (d) spatial localization of protein in a compartment or shuttling of proteins between compartments...

Ngày tải lên: 07/03/2014, 16:20

... application of a newly developed technique for specific labelling of recombinant proteins by means of incorporating a radioactive selenocysteine residue in a C-terminal Sel-tag [18] Der p 2 carrying the ... recombinant Der p 2 carrying a novel C-terminal tetrapeptide Sel-tag enabling labelling with the gamma-emitting radionuclide 75Se at a single selenocysteine residue ([75Se]Der p 2) In vivo tracking ... homeostasis in the air-way mucosa is maintained in order to prevent respirat-ory infections as well as allergic manifestations However, in an increasing proportion of the popula-tion in industrialized...

Ngày tải lên: 07/03/2014, 21:20

... al [22] obtained quantitative in vitro images of nine transcripts that were predicted to be miRNA9 targets using the ensemble machine-learning algorithm However, in vivo imaging of miRNA regulation ... the in vitro and in vivo investigations of miRNA production patterns Second, noninvasive monitoring of miRNAs provides useful information of the possible role of miRNAs involving cellular developments ... imaging sys-tems that are used to create a better understanding of the production and function of miRNA in vivo as well as for monitoring the therapeutic potential of miRNAs in cancer Bioimaging...

Ngày tải lên: 16/03/2014, 01:20

... allocation, ment of the cost of debt and adequate pricing of new bond issuances For example, our find-ings help private investors to better understand the underlying risks of bond indices and bond ... based on the total performance of individual bonds included in the corresponding bond index The total performance is defined as monthly bond price changes plus monthly accrued interests plus monthly ... employed in financial research to analyze the term structure of interest rates by Litterman and Scheinkman (1991) Recently, PCA has gained importance in a wide array of applications in finance...

Ngày tải lên: 29/03/2014, 06:21

... previously to induce an increase in the level of [Ca2+]i and a correlated calpain activation [28] This increase in [Ca2+]i is more intense in aorta than in brain Under these conditions, in brain, no ... concerns the capacity of HSP90 to protect NOS in vivo against proteolytic Fig 4 In vivo digestion of NOS and HSP90 in NMS rats during HSD treatment Aliquots (20 lg protein) of rat brain soluble material ... 6B) Indeed, approximately 80–90% of eNOS protein and A B Fig 2 Levels of calpain isoforms and calpain substrates in the aorta of NMS and HMS rats treated with HSD Aliquots (100 lg protein) of...

Ngày tải lên: 30/03/2014, 04:20

... known as kinase-insert domain-containing receptor (KDR) [2] In animal models of chronic ischemia, manoeuvres that increase VEGF levels by intramuscular injection or vascu-lar infusion of adenoviral ... [3,5-7] In spite of all, clinical trials using gene or protein therapy with VEGF isoforms for treatment of myocardial or peripheral ischemia have been somewhat disappointing indicating the needs ... hematoxylin and eosin [4] Quantitative analysis was done by counting the total number of endothelial cells, identified by lectin staining (see immu-nohistology), in the Matrigel plug in each of 20...

Ngày tải lên: 18/06/2014, 15:20

... murine receptor resulted in a faint stain of the cytoplasm and rarely in staining of the membrane of the tumor cells (Figure 3B) In the more advanced 30 weeks tumors the staining patterns changed ... activity of the hTid proteins occurs through the interference with multiple oncogenic pathways by binding and modifying functions of key molecules maintaining intracellular sig-nalling The ErbB-2 ... characterized by positive staining ranging from moderate to intense staining In the context of the potential association of htid expression with the biology of human breast cancers of different subtype...

Ngày tải lên: 18/06/2014, 16:20

... prove the feasibility of detecting several forms of femoral implant loosening, in vitro and in vivo using techniques based on harmonic distortion [16-19] In vitro, the analysis of frequency response ... technique for the non invasive post operative assessment of prosthesis fixation, in view of detection of loosening Competing interests The authors declare that they have no competing interests Authors' ... sys-tem for intra operative manufacturing and stability testing of hip prostheses In Proceedings of TEHNOMUS XIII, the 13th Inter-national conference on New technologies and products in machine manu-facturing...

Ngày tải lên: 20/06/2014, 01:20

... model of autoimmunity Transient autoimmunity can be induced in mice by the adminis-tration of a single high dose of LPS This induces a pulse of nucleosomes and chromatin in the plasma within the ... protein and the level of free G-actin in the circulation at sites of inflammation inhibiting the DNase I activity Our data obtained by a transgenic expression of the mutant protein (ash.DNase I) in ... characterized a number of DNase I mutants, including ones resistant to inhibition by G-actin Mutations were also intro-duced to increase the affinity of the DNase I for DNA, resulting in two improved...

Ngày tải lên: 09/08/2014, 07:20

... number of dose-contributing catheters increases There-fore, the catheter contribution index increases with the number of contributing catheters In case of a single con-tributing catheter,IP= 0 In ... mm and of interstitial edema (T2-w images) -5 6 ± 6.0 mm in plane, indicating a shift of the irradia-tion effect volume against the corresponding tolerance dose volume in the direction of the ... is≈ - 5 mm in plane, indicating a shift of the irradiation effect in the direction of the catheter entry site The shift is within the slice thickness of 5 mm of the treatment planning CT but...

Ngày tải lên: 09/08/2014, 09:21

... Site Directed in-vivo Delivery of Drugs and Vaccines Anil Mahapatro 1 and Dinesh K Singh 2 * 1Bioengineering Program & Department of Industrial and Manufacturing Engineering, Wichita State ... transition of materials from liquid to gel due to ionic interaction at room temperature An example of preparation of gelatin NPs includes hardening of the droplets of emulsified gelatin solution into ... producing the antigenic protein within the vicinity of professional antigen presenting cells to initiate immune response Such vaccines produce both humoral and cell-mediated immunity because intracellular...

Ngày tải lên: 11/08/2014, 08:20

... dissolved in DMSO and Disintegration of F-actin in EGTA and restoration of actin network prompted by calcium or magnesium ions Figure 5 Disintegration of F-actin in EGTA and restoration of actin network ... compensating wortmannin inhib-itory effects on chloroplast responses with addition of both divalent cations Actin organization in continuous light In dark-adapted cells distinctly outlined actin bundles ... activity of many cytoskeletal proteins and act as secondary messenger in several plant signalling pathways including those initiated by pho-totropins [16-19] As shown in studies employing the aequorin...

Ngày tải lên: 12/08/2014, 03:20

... with a final aliquot of virus preparation, resulting in a mul-tiplicity of infection (MOI) of 15 Cells were main-tained during 48 hours before proceeding with transplantation Transplantation of BMC ... removal of the drug wouldresult in the reduction in vector expression The insertion of the retroviral vector may induce the unwanted expression of a neighboring oncogene In the case of pCLPG, ... transduction, as indicated) in 100 μl of 1× PBS In parallel, irradiated animals were maintained under the same conditions, but without having received a BMC injection, in order to serve as a control of the...

Ngày tải lên: 12/08/2014, 04:21

... upregulate expression of PD-L1, iNOS, and COX-2 in MSCs, making these molecules candidate mediators of T-cell inhibition The involvement of iNOS and COX-2 in inhibition of T-cell proliferation ... addition of inhibitors of these enzymes - GW274150 and indomethacin [8,36], respec-tively - to the co-cultures The addition of these inhibi-tors resulted in the abrogation of the inhibition of T-cell ... effect of MSCs on the disease course of CIA, mice were injected intrave-nously or intraperitoneally with 1 × 106MSCs in 100μl of sterile PBS at the indicated time points Controls received injections...

Ngày tải lên: 12/08/2014, 11:23

... rhIL-18 (2.5 nM) probing for phospho-ERK (n=4) *p < 0.05 1/2 p-p38 total p38 0 min 1min 5 min 15 min 30 min 45 min p-ERK½ total ERK½ 0 min 1min 5 min 15 min 30 min 45 min (n=5) * p < ... reductions in joint inflammation during ZIA compared with Wt mice Many proinflammatory cytokines were reduced in IL-18 gene-knockout mouse joint homogenates during ZIA, including macrophage inflammatory ... mAb) (f ) Murine LN show-ing recruited cells (red fluorescent stainshow-ing, see arrow) (g) Merger of (e) and (f ) showing a lack of nonspecific cel-lular staining (h) DAPI staining showing cell...

Ngày tải lên: 12/08/2014, 14:22

... in significant numbers of individuals tested (60.7% of HAM/TSP patients, 34 out of 56; 71.1% of HCs, 27 out of 38; 30.0% of ATL patients, 3 out of 10), whereas HTLV-1 HBZ mRNA was expressed in ... 6Comparison of HBZ mRNA load with tax mRNA load among HTLV-1 infected individuals in different clinical status To investigate the mutual expression status of HBZ and tax mRNA in different clinical ... (27 out of 38) of HCs and 30.0% (3 out of 10) of ATL patients, whereas HTLV-1 HBZ mRNA was expressed in all the infected individuals tested As shown in figure 3, HBZ mRNA/tax mRNA ratio in PBMCs...

Ngày tải lên: 12/08/2014, 23:20

... (during and after GCV therapy) of tumor volumes and integrated light intensity units within a region of interest (ROI) including all tumors were plotted against each other (Figure 5) Within the ... to insufficient transgene delivery to tumor cells Therefore, this study aimed at developing a strategy for real-time noninvasive in vivo monitoring of the activity of a therapeutic gene in brain ... correlation of both enzyme activities Serial optical imaging of tumor bearing mice detected in all cases GCV induced death of tumor cells expressing the fusion protein and proved that bioluminescence...

Ngày tải lên: 14/08/2014, 19:22

... elements in vitro and in vivo Based on in vivo mutational analyses, two tandem ISL1 binding sites Trang 5v are required for enhancer activity in the pituitary and spine and a PITX1 binding site ... member of the LIM-HD family of transcription factors LIM-HD transcription factors contain two zinc finger LIM domains important for protein-protein interactions and a central DNA binding homeodomain ... adaptation in lower vertebrates The human intermediate lobe is less pronounced than in other vertebrates consisting of only a thin layer of cells Because of the diminutive size of the human intermediate...

Ngày tải lên: 24/08/2014, 11:06

... ≥400 base pairs in the juxtamembrane d omain, are found in 20-25% of adult AML cases.1-3 In addition, activating point mutations in the second kinase domain occur in about 7% of adult AML patients.4 ... to FLT3 inhibitors in MV4-11-R 56 3.3.3 Identification of enhanced activation of STAT pathways and overexpression of survivin in the resistant lines 58 3.3.4 Upregulation of survivin in MV4-11-R ... 3.3.7 Indirubin derivative (IDR) E804 induced apoptosis through inhibition of STAT pathway and survivin and sensitized 3.3.8 Survivin was a direct target of STAT3 3.3.9 In vivo efficacy of IDR...

Ngày tải lên: 11/09/2015, 09:06