in vitro testing of handling technical complicati

... structure of dinuclear Cu (OAc) (H O) (right). Trang 3The difference between the amount released at 48 h andthat contained in the original loading solution deter-mined Cu loading, resulting in values ... added competing ligand, (D) release as a function of pH in the absence of competing ligand effects. Trang 5experimental findings Table 1 contains a summary ofCu weight percents determined for each ... file 4: In vitro toxicity for comparison of Cu in CuCNPs versus similar dosing of free Cu(OAc) 2 graph showing copper contained in nanoparticles was more toxic than an equivalent amount of copper

Ngày tải lên: 21/06/2014, 02:20

... albumin IN VITRO MATURATION OF OOCYTES IN INFERTILITY TREATMENTS The research into maturation of immature oocytes initiated by Pincus and Enzmann (8) and continued by Edwards et al (23) was not incorporated ... have been reported in many species including humans (11) The actions of endocrine, paracrine, and autocrine factors that control oocyte maturation in vitro, either directly or indirectly, are mediated ... donors Indeed, results of a recent survey indicate that three-quarters of potential donors changed their mind about donating after receiving information on the procedures involved (48) Avoiding

Ngày tải lên: 05/08/2014, 16:20

... III). Mineral content of leaves The mineral content of the leaves varied according to the gelling agent and the peri-od of time in culture A significant accumu-lation of Na was found in the ... from repetitive use of agar Mineral content of gelling agents The 2 gelling agents presented major dif-ferences in mineral content (table II) Gel-rite contained a higher amount of Ca and Mg and ... gradient was 15-40% B in A for 20 min, 40-60% B in A for the next 5 min, then 60-100% B in A for 3 isocratically min; rate was 1 ml min Peaks were recorded at 250 nm The naphthoquinones hydrojuglone

Ngày tải lên: 08/08/2014, 23:22

... to depletion of glucose in the incubation medium Previous studies investigating in vitro FDG uptake in various cell lines have revealed that hypoglycemic condi-tions lead to an increased FDG ... induce in vitro hypoxia The GOX/CAT sys-tem has been employed in the past in various studies In particular, Baumann et al have applied the enzy-matic system for investigation of the effects of ... defined O2 concentration The conditions forin vitro generation of hypoxia at a level of 2% were carefully chosen in concert with the results of previous studies In particular, evaluation of oxygen

Ngày tải lên: 09/08/2014, 09:20

... matured in the presence of inflammatory stimuli com-prises pro-inflammatory cytokines and chemokines, including the T cell inhibitory cytokine IL-10, the Th-1 promoting cytokine IL-12, as well as ... cells, excluding the CD209+MDDC (stimulator cells), with decreased CFSE staining intensity resulting from dilution during cell division (viz., the fluorescence intensity of membrane staining halves ... Mixtures of peptides con-sisting of 15 amino acid residues, overlapping by 11 amino acids each, were designed to span the sequences of CMV pp65, CEA, MAGE-3, and the intracellular domain (ICD) of HER-2/neu

Ngày tải lên: 11/08/2014, 10:23

... cell line easier than another, thus possibly produ-cing some replication inhibition in one cell line and not the other Further testing is needed to identify any of these contributing or limiting ... attachment/entry inhibition assays This is particularly pertinent in confined marine habitats that can be seeded with the active elements of these extracts in hopes of preventing the spread of viral ... of inhibition of VSV plaque formation than HSV-1 plaque formation, in Vero cells This phenomenon may be attributed to the nature of the envelope proteins of rhabdoviruses When comparing the inhibitive

Ngày tải lên: 12/08/2014, 04:20

... sur-faces, allowing for a controlled comparison of sperm binding Thus, the variation in binding capacity between individual ZP is eliminated The binding capacity of two semen samples to matching hemizonae ... General-purpose cellular stains (Wright’s, Giemsa, haematoxylin-eosin, India ink) can be used (Varner et al 1991b), but live-dead stains (aniline-eosin, eosin-nigrosin, eosin-fast green) are more ... detect acrosome integrity is staining with fluorescein-conjugated lectins, such as Peanut Agglutinin (PNA), Pisum Sativum Agglutinin (PSA) or Concanavalin A (ConA) coupled with fluores-ceinisothiocynate

Ngày tải lên: 12/08/2014, 15:20

... no competing interests SC declare no competing interests LB declare no competing interests SR declare no competing interests MM declare no competing interests EC declare no competing interests ... concentrations inhibiting 50% of active force in carbachol-contracted human bronchial rings Figure 5 Salbutamol concentrations inhibiting 50% of active force in carbachol-contracted human bronchial rings ... β2-AR desensitization in HASMC, thus ruling out the possibility of the involve-ment of this protein kinase Indeed, H89 tended to enhance the response to isoproterenol both in LTD4 -chal-lenged

Ngày tải lên: 12/08/2014, 16:20

... Cruz, CA, USA) Anti-actin antibody was raised against a peptide mapping at the C-terminus of actin of human origin and was reactive against both actin isoforms Antibody against type I collagen ... p38 (p38 Mitogen Activated Protein Kinase), involved in IL-6 (Interleukin-6) and IL-8 (Interleukin-8) production A) Inhibition of IL-6 and IL-8 synthesis by the p38 inhibitor SB 203580 Human chondrocytes ... 15ΔPGJ2production is induced by MGDG treatment in IL-1a treated cells There is increasing evidence of COX-2 involvement in the resolution of inflammation via its product 15ΔPGJ2 [18,19] Since in the same

Ngày tải lên: 12/08/2014, 17:21

... (1.25 – 5 mM) instead of NAC (data not shown) In contrast, in NCI-H292 cells but not in PBEC, depleting GSH using BSO resulted in a full inhibition of wound repair in presence of 1 AU/ml that ... is in line with the proposed role of this MAP kinase in cell proliferation In the presence of NAC, CSC did not induce ERK1/2 acti-vation, indicating that this process involves the action of oxidants/free ... decrease in GSH, but did not increase GSH in the absence of CSC This finding is in line with previous observations, showing that NAC does increase total non-protein thiols but does not increase

Ngày tải lên: 12/08/2014, 18:20

... factor-alfa; IFNg, interferon-gamma; IL-1a, interleukin-1 a; IL-4, interleukin-4; IL-6, interleukin-6; IL-10, interleukin-10; IL-17, interleukin 17; IL-22, interleukin-22; CRP, C-reactive protein Extreme ... diarrhea (p < 001), which is interesting given that IL-22 appears to play an important role in maintaining the integrity of the epithelium in animal models of intestinal infection [63] It has been ... accounting for more than 50% of HIV-1 infections worldwide in 2004 [1] It predominates in countries with 80% of all global HIV-1 infections (sub-Saharan Africa, India) and is rapidly increasing in

Ngày tải lên: 12/08/2014, 23:23

... Kalpana1,4,5 1Department of Genetics, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY, 10461, USA 2Department of Nuclear Medicine, Albert Einstein College of Medicine, 1300 Morris ... 3Department of Developmental and Molecular Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY, 10461, USA 4Albert Einstein Cancer Center, Albert Einstein College of Medicine, ... it inhibits the growth of RTs in vitro and in vivo in a xenograft mouse model with efficacy correlated with down-modulation of Cyclin D1 [7] Several reports indicate that synthetic analogs of

Ngày tải lên: 13/08/2014, 18:21

... 1Research articleIN VITRO CULTURE OF PETIOLE LONGITUDINAL THIN CELL LAYER EXPLANTS OF VIETNAMESE GINSENG (PANAX VIETNAMENSIS HA ET GRUSHV.) AND PRELIMINARY ANALYSIS OF SAPONIN CONTENT Duong ... with similar saponin profiles between the resulted in vitro tissues and plants grown in nature, suggest its potential use in generating Vietnamese ginseng in large amount for medicinal purpose Key ... and the effects of exogenous spermidine and proline on enhancement of somatic embryogenesis (Nhut et al., 2012b) from in vitro main roots tTCLs of P vietnamensis were investigated In the present

Ngày tải lên: 20/04/2015, 21:33

... Trang 1IN VITRO STUDY OF SURFACE FUNCTIONALIZATION OF TITANIUM SUBSTRATES FOR POTENTIAL ENHANCEMENT OF OSSEOINTEGRATION AND REDUCTION OF BACTERIAL INFECTION HU XUEFENG NATIONAL UNIVERSITY OF SINGAPORE ... involved in osteoclast activation and induce the differentiation of macrophages and monocytes into osteoclasts, which inhibit osseointegration (Cobelli et al., 2011) In addition, during the inflammatory ... cause severe pain and degradation in bone functions as a result of excessive loading or lack of normal biological self-healing process With the rapid increase in the aged population in recent years,

Ngày tải lên: 10/09/2015, 09:13

... after SIT In vitro HDM challenge presents concordant patterns with in vivo findings: 1) increase of Th2 and Th17 response in AR patients; 2) suppression of IL10 producing CD4+T cells in SIT-untreated ... treatment were used in the following in vivo and in vitro studies 3.2 In vivo Study mRNA/protein levels of Th17 associated genes and specific cytokines of Th cell subsets were measured in PBMCs from ... subjects; while the increase of Th2 but decrease of Th1 cells was also determined in all AR’s PBMCs These results show a concordance between in vivo and in vitro findings, confirming that not only

Ngày tải lên: 23/09/2015, 14:47

... DEVELOPMENT OF A BIOREACTOR FOR IN-VITRO ENGINEERING OF SOFT TISSUES KYAW MOE NATIONAL UNIVERSITY OF SINGAPORE 2005 Trang 2 DEVELOPMENT OF A BIOREACTOR FOR IN-VITRO ENGINEERING OF SOFT TISSUES ... delay in maturation because of stress shielding [Kumar et al, 1999] 2.6 Tissue Engineering Tissue engineering has been defined as “an interdisciplinary field that applies the principles of engineering ... initial injury while never regaining the properties of the normal tendon/ligament, thus demonstrating the need for tissue engineering approaches During the course of healing, the level of pain decreases

Ngày tải lên: 04/10/2015, 15:46

... people In this research, in vitro propagation of stevia, was carried out in 4 experiments (callusing, shooting, rooting, and acclimatization) The results supported that MS medium (including vitamins) ... not maintain the callus growth since all explants continued to turn brown and died out The maintenance of callus plays an important role in in vitro propagation through callusing Good maintenance ... in plants, their uptake, type of Auxins and Cytokinins used and their mode of action (Gupta et al., 2010) A concerned problem was the browning of callus In treatments such as S2 or S3, browning

Ngày tải lên: 06/10/2015, 13:04

... evaluated in clinical trials (at a dosage of 52.5 mg) It was subsequently withdrawn from clinical testing as a result of increased risk of sexually transmitted infection due to disruptions in the vaginal ... cervical-vaginal tissues before being considered as a candidate for evaluation in clinical trials It must not cause inflammation or sloughing of the vaginal epithelium, and even minor, subclinical ... lemon juices and white household vinegar in a highly standardized in vitro testing algorithm that is currently supported by the National Institute of Allergy and Infectious Diseases (NIAID) to identify...

Ngày tải lên: 10/08/2014, 05:21

... also protein fragments for mapping of interaction domains The use of in vitro expression approaches to analyze protein products is likely to gain momentum for the analysis of known proteins as well ... functionally analyzed using in vitro assays Furthermore, rapid in vitro protein–protein interaction studies could be performed using this technique where not only full-length version of proteins could be ... original primers Of course in this case the use of an enzyme with 3′-exonuclease proofreading activity should be avoided to protect the differentiating 3′-end An example of the design of original...

Ngày tải lên: 25/10/2013, 22:20

... reaction by using 32P-labeling primer or template in the presence or absence of DNA-binding agents (DDIs and doxorubicin) The DNA bulge binding of both compounds was detected by CD and UV melting experiments ... is formed To gain insight into the binding of drugs to bulge DNA, several bulgecontaining oligonucleotides were selected as binding hosts for DDI-1A and DDI-1B CD spectroscopy of DDI-1A (Fig ... two-base bulge in DNA via stacking of its helically oriented aromatic ring systems on the bulge-flanking base pairs that define the long sides of the triangular prism binding pocket, with its amino sugar...

Ngày tải lên: 07/03/2014, 06:20