in vitro studies of sulfur mustard toxicity

... By keeping all these points in mind management of this disease has become the need of the hour Earlier many workers reported management of Sclerotinia rot of mustard by the application of fungicides ... incidence of white rot of rapeseed and mustard incited by Sclerotinia sclerotiorum in Punjab Plant Dis Res 15: 232-233 Kolte, S J (1985) Rapeseed-mustard and sesame diseases, In: Diseases of ... substitute of chemicals in managing scletotinia rot It will not only help in reducing hazardous effects of chemicals on environment and human health but will also ensure sustainable development of agriculture

Ngày tải lên: 11/03/2020, 11:33

... α‑Terpinene, (44) γ‑Terpinene, (45) 4‑Terpineol, (46) α‑Terpineol, 47) β‑Terpineol, 48) γ‑Terpineol, (49) Terpinolene, (50) Thymol Trang 4Insect cultures and rearing conditionsLarvae of Cx quinquefasciatus ... estimate the strength of the protein–ligand interaction, to deter-mine biding poses and free energy values [19] Docking describe ligand binding to a receptor through noncova-lent interactions which ... [40] Docking analysis was done using the AutoDock4 software [41] For the docking the active site was defined considering the residues within a grid of 60 A° × 60 A° × 60 A° centered in the active

Ngày tải lên: 29/05/2020, 13:36

... cancer cell line pairs consisting of a cisplatin-sensitive parental line and a stable cisplatin-resistant sub-line derived byin vitro selection with cisplatin Human ovarian carcinoma cell lines, OVACAR-3, ... Cisplatin - Mean ± SD. IC 50 values for Cisplatin in isogenic ovarian cancer cell line pairs consisting of Cisplatin-sensitive parental lines and stable Cisplatin-resistant sub-lines derived by in vitro ... effects of BT on ovarian cancer cell lines As shown in Figure 1, treatment with increasing concen-trations of BT resulted in dose dependent reduction in cell viability in all the cell lines tested

Ngày tải lên: 05/11/2020, 01:03

... cell lines and/or due to the method used to induce Se deficiency in our in vitro model. In studies employing Jurkat E6 cells, the Se-deficient medium comprised of insulin, transferrin and bovine ... Equal loading of protein was validated by re-blotting the same membranes with anti-β-actin The data is representative of three independent experiments (B) Increase in the expression of MnSOD in Se- ... effect of Se on viral infection Se deficiency increases apoptosis in WNV-infected Vero cells Vero cells were inoculated with WNV NY99 strain at mul-tiplicity of infection (MOI) 1, at day 7 post-induction

Ngày tải lên: 20/06/2014, 01:20

... article In vitro suppression of the MMP-3 gene in normal and cytokine-treated human chondrosarcoma using small interfering RNA Address: 1 Bone and Joint Research Laboratory, Department of Veterinary ... 5 min of incubation with 0.4% Trypan blue, the percentages of stained cells (indicative of nonviable cells) versus stain-excluding cells were counted in a hemocytometer Then the percentage of ... have definite effects on NO production in the joints These inhibitory effects of HA on cell apop-tosis may play a role in its mechanism of action in chon-droprotection [38] Our findings indicated

Ngày tải lên: 20/06/2014, 04:20

... albumin IN VITRO MATURATION OF OOCYTES IN INFERTILITY TREATMENTS The research into maturation of immature oocytes initiated by Pincus and Enzmann (8) and continued by Edwards et al (23) was not incorporated ... have been reported in many species including humans (11) The actions of endocrine, paracrine, and autocrine factors that control oocyte maturation in vitro, either directly or indirectly, are mediated ... donors Indeed, results of a recent survey indicate that three-quarters of potential donors changed their mind about donating after receiving information on the procedures involved (48) Avoiding

Ngày tải lên: 05/08/2014, 16:20

... III). Mineral content of leaves The mineral content of the leaves varied according to the gelling agent and the peri-od of time in culture A significant accumu-lation of Na was found in the ... from repetitive use of agar Mineral content of gelling agents The 2 gelling agents presented major dif-ferences in mineral content (table II) Gel-rite contained a higher amount of Ca and Mg and ... gradient was 15-40% B in A for 20 min, 40-60% B in A for the next 5 min, then 60-100% B in A for 3 isocratically min; rate was 1 ml min Peaks were recorded at 250 nm The naphthoquinones hydrojuglone

Ngày tải lên: 08/08/2014, 23:22

... to depletion of glucose in the incubation medium Previous studies investigating in vitro FDG uptake in various cell lines have revealed that hypoglycemic condi-tions lead to an increased FDG ... defined O2 concentration The conditions forin vitro generation of hypoxia at a level of 2% were carefully chosen in concert with the results of previous studies In particular, evaluation of oxygen ... induce in vitro hypoxia The GOX/CAT sys-tem has been employed in the past in various studies In particular, Baumann et al have applied the enzy-matic system for investigation of the effects of

Ngày tải lên: 09/08/2014, 09:20

... matured in the presence of inflammatory stimuli com-prises pro-inflammatory cytokines and chemokines, including the T cell inhibitory cytokine IL-10, the Th-1 promoting cytokine IL-12, as well as ... used in vaccine-based clinical studies for a variety of cancers In order to assess whether in vitro differentiated MDDC from cryopreserved PBMC of cancer patients are functionally distinct from ... cells, excluding the CD209+MDDC (stimulator cells), with decreased CFSE staining intensity resulting from dilution during cell division (viz., the fluorescence intensity of membrane staining halves

Ngày tải lên: 11/08/2014, 10:23

... attachment/entry inhibition assays This is particularly pertinent in confined marine habitats that can be seeded with the active elements of these extracts in hopes of preventing the spread of viral ... inhibition of CCV replication in CCO up to 48 hr post-infection, which decreased slightly by 84 hr post-infection All remaining extracts tested against CCV in CCO cells were determined to present ... of inhibition of VSV plaque formation than HSV-1 plaque formation, in Vero cells This phenomenon may be attributed to the nature of the envelope proteins of rhabdoviruses When comparing the inhibitive

Ngày tải lên: 12/08/2014, 04:20

... sur-faces, allowing for a controlled comparison of sperm binding Thus, the variation in binding capacity between individual ZP is eliminated The binding capacity of two semen samples to matching hemizonae ... General-purpose cellular stains (Wright’s, Giemsa, haematoxylin-eosin, India ink) can be used (Varner et al 1991b), but live-dead stains (aniline-eosin, eosin-nigrosin, eosin-fast green) are more ... detect acrosome integrity is staining with fluorescein-conjugated lectins, such as Peanut Agglutinin (PNA), Pisum Sativum Agglutinin (PSA) or Concanavalin A (ConA) coupled with fluores-ceinisothiocynate

Ngày tải lên: 12/08/2014, 15:20

... no competing interests SC declare no competing interests LB declare no competing interests SR declare no competing interests MM declare no competing interests EC declare no competing interests ... possibly involving PKA However, in previous studies we found that the reduction of relaxant response to salbutamol in allergen-challenged rings was not prevented by inhibition of prostaglandins [23], ... concentrations inhibiting 50% of active force in carbachol-contracted human bronchial rings Figure 5 Salbutamol concentrations inhibiting 50% of active force in carbachol-contracted human bronchial rings

Ngày tải lên: 12/08/2014, 16:20

... Cruz, CA, USA) Anti-actin antibody was raised against a peptide mapping at the C-terminus of actin of human origin and was reactive against both actin isoforms Antibody against type I collagen ... p38 (p38 Mitogen Activated Protein Kinase), involved in IL-6 (Interleukin-6) and IL-8 (Interleukin-8) production A) Inhibition of IL-6 and IL-8 synthesis by the p38 inhibitor SB 203580 Human chondrocytes ... 15ΔPGJ2production is induced by MGDG treatment in IL-1a treated cells There is increasing evidence of COX-2 involvement in the resolution of inflammation via its product 15ΔPGJ2 [18,19] Since in the same

Ngày tải lên: 12/08/2014, 17:21

... (1.25 – 5 mM) instead of NAC (data not shown) In contrast, in NCI-H292 cells but not in PBEC, depleting GSH using BSO resulted in a full inhibition of wound repair in presence of 1 AU/ml that ... is in line with the proposed role of this MAP kinase in cell proliferation In the presence of NAC, CSC did not induce ERK1/2 acti-vation, indicating that this process involves the action of oxidants/free ... observation that the EGFR tyrosine kinase inhibitor AG1478 blocks CSC-induced phosphorylation of ERK1/2 is in line with studies showing a role of EGFR in cigarette smoke induced epithe-lial cell activation

Ngày tải lên: 12/08/2014, 18:20

... and reproduction in any medium, provided the original work is properly cited. Abstract Introduction The design of clinical trials of interventions aimed at reducing mortality in patients with ... relative treatment effect of the intervention is independent of the patients' risk for death We reviewed published data from phase III clinical studies of severe sepsis to determine whether a relationship ... 2004 Studies were included in this analysis if they met the following criteria: randomized, double blind, placebo controlled clinical trial; enrollment of adult patients who met the diagnosis of

Ngày tải lên: 12/08/2014, 22:22

... (Fig 3E) Interestingly, the inclusion of IKK2 inhibition by SC-514 with our experimentally determined rate constants in the original model resulted in a delay in simulated peak and damping of subsequent ... simulated in silico Combining this method of analysis (in vitro, cell-based and in silico analysis) will facilitate systematic understanding of the underlying properties of this signaling pathway ... = Intensity of nucleus Intensity of cytoplasm Intensity of nucleus Scheme This is a simplication of a cell as seen by analysis software, where the software measures the intensity of NF-jB in...

Ngày tải lên: 16/03/2014, 11:20

... treatment were used in the following in vivo and in vitro studies 3.3 In vitro Study To mimic the functional response of Th17 and other Th cell subpopulations to HDM allergens, an in vitro allergen ... between in vivo and in vitro findings, confirming that not only Th2, but also Th17 cells play an important role in the context of allergic inflammation IL10 producing CD4+ T cells showed a distinct ... markers in each study group tested in vitro 3.2 In vivo Study 3.3.1 Th17 specific gene mRNA/protein expression Following a 3-day in vitro stimulation, there were mRNA/protein levels of Th17 associated...

Ngày tải lên: 23/09/2015, 14:47

... use of FCS-containing media during genistein incubation and the incubation with higher genistein doses Mechanisms of interaction To search for mechanisms of interaction we investigated protein ... Genistein, a tyrosine kinase inhibitor, enhanced radiosensitivity in human esophageal cancer cell lines in vitro: possible involvement of inhibition of survival signal transduction pathways Int ... after incubation with genistein concentrations of 15 – 30 μM in FCS-containing media [41] In this study the NF-κB activity was investigated, too An inhibition of radiationinduced activation of NF-κB...

Ngày tải lên: 09/08/2014, 09:22

... an increase in scattering within the material Light scattering in a material is the result of scattering centers that cause the incident light to be scattered in all directions The scattering ... rate of 94% in a five year clinical study27 This crown system is composed of a densely sintered, high-purity aluminum oxide coping that is combined with the low-fusing AllCeram veneering porcelain17 ... composite core materials of varying thickness Table 4.2 The order of preference of composite cores in masking the posts 57 60 viii SUMMARY The aims of these in- vitro studies are to evaluate the...

Ngày tải lên: 09/10/2015, 11:24

... mustard- induced cutaneous inflammation and blistering (2008-K.Kehe) (3) Medical aspects of sulfur mustard poisoning (2005- Kai Kehe, Ladislaus Szinicz) (4) Dermal Toxicity of Sulfur Mustard (2009 ... absorbed into the skin • SM is a well-known carcinogen for human References (1) Acute effects of sulfur mustard injury – Munich experiences (2009 – K.Kehe) (2) Molecular toxicology of sulfur mustard- induced ... Frequently observed finding accompanying all SM skin lesions Example • Here is the onset of symptoms in 10 Iranian patients who were treated during 1984 and 1985 in hospitals in Munich, after exposure...

Ngày tải lên: 28/05/2016, 09:37