in vitro models of development and disease

... AccessInhibition of MMP-2 and MMP-9 decreases cellular migration, and angiogenesis in in vitro models of retinoblastoma Anderson H Webb1†, Bradley T Gao1†, Zachary K Goldsmith1†, Andrew S Irvine1, ... promising results Taken together, we provide a comprehensive in vitro analysis of MMP-2 and MMP-9 activity in Rb in several checkpoints that are deregulated in cancer Our findings provide initial ... Rodriguez-Galindo C, et al Proceedings of the consensus meetings from the International retinoblastoma staging working group on the pathology guidelines for the examination of enucleated eyes and evaluation

Ngày tải lên: 06/08/2020, 07:35

... crystalline structure and create intermolecular cross-linking both in the amorphous and crystalline regions when it penetrates the granules through channels and cavities resulting in inhibiting the ... obtained from 2o 2θ to 35o 2θ with a scanning speed of 8o/min and scanning step of 0.02o [2] 2.7 Determination of thermal characteristics of starch Thermal characteristics of the native and treated ... present in crystalline and in non-crystalline regions of the granule to form new polymer chains in the heat-moisture treated starches [5] The gelatinization temperature and enthalpy of the cassava

Ngày tải lên: 16/12/2017, 18:06

... amount of money and investment duration Hee (2010) gives four components influencing green finance development, including: financing green businesses and technologies, developing green financial ... of long-term financing is the reason that many developing countries are facing in developing green finance: (i) Lack of capital and/or inappropriate financial conditions; (ii) Lack of tools and ... developing country like Vietnam should consider banking system and finances institutions as principal subjects, playing a crucial role in the green finance campaign Accordingly, banking system and

Ngày tải lên: 02/04/2022, 10:14

... amount of money and investment duration Hee (2010) gives four components influencing green finance development, including: financing green businesses and technologies, developing green financial ... of long-term financing is the reason that many developing countries are facing in developing green finance: (i) Lack of capital and/or inappropriate financial conditions; (ii) Lack of tools and ... Banks and financial institutions also can stay away from environment and social risk by offering green finance products and services As a role of lender, banks contain a huge part in direct the investment

Ngày tải lên: 02/04/2022, 10:38

... theContents of Monoamine Transmitters in the Striatum of Rats in Parkinson's Disease Acupunct res 1998;1:44–8 69 Zhu W, Xi G, Ju J Effect of acupuncture and Chinese medicine treatment on brain dopamine ... Considerations of sex and gender differences in preclinical and clinical trials Handb Exp Pharmacol 2012;214:127–47 16 Singh N, Pillay V, Choonara YE Advances in the treatment of Parkinson’s disease ... Walton-Hadlock J Primary Parkinson's disease: the use of Tuina and acupuncture in accord with an evolving hypothesis of its cause from the perspective of Chinese traditional medicine–part 2 Am J Acupunct

Ngày tải lên: 04/12/2022, 16:16

... 8PATHOGENESIS AND ROLE OF ACETYLCHOLINESTERASE IN THE TREATMENT 4 Trang 9Treatment methodso Using acetylcholinesterase inhibitor drugs (eg: tacrine, rivastigmine, galantamine and donepezil) ... exercise Trang 10II In Vitro Model Trang 11Fig 5 Schematic representation of the binding sites of AChE ES: esteratic site AS: anionic substrate binding site ACS: aromatic cation binding site PAS: ... mg/kg) plus scopolamine and compound 5l (20 mg/kg) plus scopolamine in comparison to scopolamine group. In vivo evaluation of compound 5l confirmed memory improvement in scopolamine-induced impairment.

Ngày tải lên: 26/02/2023, 19:15

... in fighting cancer, reducing heart disease and are also good for eye health and digestion (Nahar and Ullah, 2012) China is leading producer among Asian countries, followed by India In India, ... as pre-emergence and post-pre-emergence damping-off of tomato seedlings Most of these fungi can also cause cuttings to rot (Thakur and Tripathi, 2015) Management of damping off by fungicides ... study was conducted to evaluate the in vitro efficacy of native Trichoderma spp against Pythium spp and Rhizoctonia solani (Kuhn.) causing damping-off disease in Tomato One hundred eighty soil

Ngày tải lên: 14/01/2020, 05:53

... cardiomyopathy and encephalopathy [47, 48] Arginino-succinic acid is a metabolite in the main biochemical pathway of lysine It is an intermediate for the metabol-ism of lysine and sucralose Studies in rats ... (P < 0.05) In addition, the main pharmacokinetic parameters of multiple and single administration of MCS have no sig-nificant difference, indicating that the absorption of MCS in rats is constant ... 9tolerance, increase serum insulin levels, and promote isletregeneration and β cell proliferation, and inhibit β cell apoptosis [29,30] In addition, mangiferin can reduce in-sulin resistance by regulating

Ngày tải lên: 21/09/2020, 13:56

... bobbinclud-ing, head tiltinclud-ing and circling The levels of the endogenous (wild-type) huntingtin protein and the transgene product were examined by western blotting using anti-huntingtin serum ... nature of the involvement of GAPDH and the CAG expansion of the mutant huntingtin protein One such scenario is that the interaction between poly-glutamine-containing proteins and GAPDH results in ... substrate of this reaction to be increased in HD In extracts of the posterior brain region of control and HD mice in the presence of excess ATP, NAD+, inorganic phosphate and glucose, we determined

Ngày tải lên: 23/03/2014, 10:20

... Agrawal and Henderson (2002) examine the publishing and patenting output of electrical engineering, com- puter science, and mechanical engineering faculty at a major research in- stitution (MIT) and ... The increasing role of these inexperienced institutions in university patent- ing influenced the overall character of the “average” university patent In- deed, the decrease in importance and ... into one of three categories: 1) a shift in focus from “basic” to “applied” university research,® 2) a decline in quality of university inventions, and 3) a decline in the dissemination of knowledge

Ngày tải lên: 03/06/2014, 01:27

... marketization of the Ukrainian economy appears to be leading— in a meandering and contradictory fashion— to the trimming back of social welfare systems and an ongoing politics of differentiation Examining ... neoliberal language of reform that focuses on streamlining social institutions, making social service provision more “humane,” and bring-ing social insurance in line with European standards In November ... marginalisation of women in contemporary Ukraine. Honing in on differentiating processes reveals that this marginalization and disintegration is anything but natural and inevitable On the other hand, dif-ferentiation

Ngày tải lên: 04/06/2014, 17:40

... adding T cells), fixed in buffered formalin and embedded in paraffin a, b: Staining with H&E c, d: Specific brown staining of apoptotic cells by anti-caspase 3 Ab e, f: In situ end-labeling ... streptavidin for 40 min at 37° C Slides were washed and incubated with 2’, 5’-diaminobenzidine (DAB, Vector Laboratories, Burlin-game, CA) followed by counterstaining with hematoxy-lin Caspase staining ... changes of ethanol (70-95%), and air dried Sections were end-labeled by incubating with biotiny-lated dCTP, dATP, and non biotinybiotiny-lated dTTP, dGTP (0.01 mM each, Invitrogen) in the presence of

Ngày tải lên: 18/06/2014, 19:20

... [8,9] There are numerous reports of increased levels of cytokines in the brains of Alzheimer's disease patients, and in transgenic mouse models of Alzheimer's disease [10-12] However, all these ... focused on a small number of cytokines within the same sample It is not clear which cytokines are key in promoting and main-taining the inflammatory environment in the AD brain Furthermore, it is ... IL-6 and TNFα in-vivo after intra-cerebral administra-tion of fibrillar Aβ into rat brain [30] In order to further understand the correlation between the amount of Aβ and cytokine levels in the

Ngày tải lên: 19/06/2014, 22:20

... cytokines and increasing the production of factors that participate in tissue repair and wound healing [6] Anti-inflammatory, Th-2 cytokines such as IL-10 and TGFβ are associated with broad ranging ... production of nitric oxide The enzy-matic activities of both inducible NOS and arginase are solely dependent on intracellular arginine and these enzymes compete for arginine [72] The low expression of ... related chitinase genes, namely CHI3L1 and CHI3L2 are overexpressed in AD brain Both forms of chi-tinase 3-like proteins do not have enzymatic chichi-tinase activity and instead, inhibit IL-1 and TNFα-mediated

Ngày tải lên: 19/06/2014, 22:20

... cell lines and/or due to the method used to induce Se deficiency in our in vitro model. In studies employing Jurkat E6 cells, the Se-deficient medium comprised of insulin, transferrin and bovine ... deficiency increases OS in Vero cells Se is an integral part of the active site of GPx1, an enzyme In vitro response of Vero and SK-N-SH cells to Se deficiency Figure 1 In vitro response of Vero and ... virus infection, it is important to identify cell lines in which Se deficiency can be efficiently induced in vitro without compromising cell viability. Based on the Se-deficient in vitro and in

Ngày tải lên: 20/06/2014, 01:20

... Journal of NeuroEngineering and Rehabilitation 2005, 2:20 Background Disturbances in gait are symptomatic of Parkinson's disease (PD) and Huntington's disease (HD) Gait abnormalities in PD include ... forelimbs and hind limbs B Representative gait signals of the left forelimb and right hind limb of a saline-treated C57BL/6J mouse walking at a speed of 34 cm/s Duration of stride, stance, and swing ... right forelimbs of a 3NP-treated mouse demonstrating coordinated stepping, despite hind limb failure of stepping The signals of left and right hind limbs are not coordinated and reflect artefacts...

Ngày tải lên: 19/06/2014, 10:20

... cells producing TGF-β resulted in the induction of Treg in vivo and in vitro in a murine model [52] Moreover, the blockade of TGF-β reduces Treg induction by the fusion cell vaccine and enhances ... (obtained with following informed consent and approved by our institutional review board obtained) was isolated and maintained in TIL Media I medium with 10% heat-inactivated FCS, mM L-glutamine, ... each vaccination [27,33] Autologous FCs were irradiated, suspended in 0.3 ml normal saline, and underwent up to nine times vaccinations via SC injection in the left inguinal area at 2-week intervals...

Ngày tải lên: 18/06/2014, 15:20

... activation and IL8 production [25,26] CARR injection in the hind paw of the mouse is one of the most commonly used models of inflammation and inflammatory pain and it has a biphasic profile [27] ... the FERM (band 4.1, ezrin, radixin, moesin) domain of PTPH1 is necessary for the inhibition of Mek, Erk, Jnk and AP-1 and also for localization of the phosphatase on the plasma membrane of Jurkat ... WT and KO mice displayed a LPS-induced increase in TNFα and IL10; b) MCP-1 and IL6 levels were significantly increased by LPS in both WT and KO mice; dot line indicates the detection limit of...

Ngày tải lên: 11/08/2014, 03:20

... compartment is involved in the induction and organogenesis of various organs, including lung, limb, kidney, pancreas, prostate, and mammary gland In general, the process of organ induction begins with ... clinical prostate cancers and metastases indicates deregulation of several pathways, including androgen/AR, Hedgehog, MAPK and neuroactive ligand/receptor signalling [abstract] In Proceedings of ... by arrows in male and female) In females there was staining in the mesenchyme of the Mullerian duct (MD) In males, the seminal vesicle (SV) mesenchyme showed little or no staining In both sexes...

Ngày tải lên: 14/08/2014, 08:20

... human 18 induced pluripotent stem cell (hiPSC)-derived HPTC-like cells for the development of in vitro models.   19 1.4 Role of inflammation in drug-induced nephrotoxicity in humans In the development ... 103 Figure 18: Immunostaining of NF-B p65 in HK-2 cells and HPTCs 107 Figure 19: Detection of NF-B p65 in by immunoblotting in protein lysates of HK-2 cells and HPTCs ... HPTC-based in vitro model for nephrotoxicology developed here employed drug-induced increases in mRNA expression levels of the pro-inflammatory interleukins (IL)-6 and IL-8 as endpoint Pro-inflammatory...

Ngày tải lên: 30/09/2015, 06:22