in cell nmr in mammalian cells part 3

... of DNA in a single cell cycle Inactivation of CDK1 leads to re-replication of DNA in eukaryotic cells including human cells [7] Targets of the kinase in the regulation of DNA replication include ... Thr19, Ser32, Ser87 and Thr109) during the M phase in mouse FM3A cells, and CDK2 is required for phosphorylation at least at two sites (Ser3 and Ser32) during interphase in HeLa cells, suggesting that ... and named mitotic (M) cells Residual cells were collected and named G 2 cells These cells and logarithmically grow-ing cells were separated into Triton-soluble (S) and Triton-insoluble (P) fractions...

Ngày tải lên: 16/03/2014, 13:20

... trypsin-induced differential protein expression in cervical cancer cells It is interesting and important to know whether trypsine-induced protein alterations in MCF-7 cells are reproduc-ible in ... MCF-7 cells In con-trast to an instantly up-regulated VDAC1 level was shown in MCF-7 cells, a significantly enhanced VDAC1 level in Hela cells starting from 8 hr In summary, trypsinization-induced ... trypsinization-induced protein alter-ations in MCF-7 cells are mostly comparable in Hela cells In conclusion, 2D-DIGE based proteomics analysis serves as a useful tool to monitor trypsin-induced cell...

Ngày tải lên: 10/08/2014, 05:21

... mediate cell killing following binding (recently reviewed in [1]) Whilst a variety of cell types including plant cells, fungal cells and even bacteria [2] have been described as hosts, mammalian cells ... 7A-cotransfected cells, thus demonstrating that strong in vivo binding of the destabilized 13R4-d1-EGFP pro-teins allows them to be maintained in the cells To rule out the possibility that the EGFP part ... expressed intracellularly This may be due to the redu-cing conditions existing in cell compartments, which may prevent the formation of intradomain disulfide bonds within the VH and VL domains resulting...

Ngày tải lên: 19/02/2014, 18:20

... transfected into 293, CHO, CV1 and 3T3 cells using superfect lipid reagent The transfected cells were grown in the medium containing 0, 0.1, 1, 5, 10 and 50 lM concentrations of RG-102240 The cells ... transfected into 3T3 cells using superfect lipid reagent The transfected cells were grown in the medium containing 0, 0.2, and lM concentrations of RG-102240 The cells were harvested at 48 h after adding ... transfected into 3T3 cells using superfect lipid reagent The transfected cells were grown in the medium containing 0, 0.2, and lM concentrations of RG-102240 The cells were harvested at 48 h after adding...

Ngày tải lên: 16/03/2014, 12:20

... (2007) 3392–3404 ª 2007 The Authors Journal compilation ª 2007 FEBS 3403 DIF-1 promotes glucose uptake in mammalian cells 32 33 34 35 W Omata et al lines is inversely correlated with tumor cell ... erythrocytes [32], Swiss 3T3 fibroblasts [33], and rat epithelial cells [34] However, neither Tg, nor A23187 ([Ca2+]c-increasing agents), nor 8-MIBMX (PDE1 inhibitor) promoted glucose consumption in 3T3-L1 ... that: (a) DIFs increase [Ca2+]c in some tumor cells [9–12], (b) DIFs activate Akt ⁄ protein kinase B in human leukemia K562 cells [13], (c) DIF-1 inactivates STAT3 in gastric cancer cells [15],...

Ngày tải lên: 23/03/2014, 09:20

... with the core+1 ⁄ S protein being mainly membrane associated Both proteins show partial endoplasmic reticulum (ER) distribution in interphase cells, and in dividing cells they also localize to ... core+1 protein in mammalian cells have failed, including transfection of cells with plasmid DNA encoding the core sequence or infection with recombinant herpes simplex virus expressing the core–E1–E2 ... protein have been limited by the fact that its detection in mammalian cells and in the HCV infectious system is elusive This study shows that intracellular levels of the core+1 protein in mammalian...

Ngày tải lên: 30/03/2014, 03:20

... reduction in turbulence quantified using turbulence kinetic energy as shown in figure 13 It was found that the turbulent kinetic energy decreases both near the Hartmann and side walls with increase in ... walls did not increase for the case with insulating walls as Trang 6in the case with conducting walls showing the contribution of the ‘M’ shaped velocity profile in the Nusselt number increase near ... that intrude into the Hida Belt (Matsuda et al 1998) Error bars show 2σ uncertainty in age Trang 163 Thermal modelling associated with frictional heating and estimation of frictional strength In...

Ngày tải lên: 19/06/2014, 10:20

... Kazakhstan 1 Introduction Promising application of thin-film technology is the synthesis of SiC, possessing such point (2830°C), wide bandgap (2.3–3.3 eV), etc (Lindner , 2003) Unfortunately, since ... correlation-energy, Phys Rev B 45(23): 13244–13249. Rohmfeld, S., Hundhausen, M & Ley, L (1998-I) Raman scattering in polycrystalline 3C-SiC: Influence of stacking faults, Phys Rev B 58: 9858–9862. ... 29: 736. Dolloff, R T (1960) WADD Tech Report 60-143: 1–22. Fromm, E & Gebhardt, E (1976) Gase und Kohlenstoff in Metallen, II Daten, Spirnger-Verlag, pp 730–33 Giardini, A A & Tydings,...

Ngày tải lên: 19/06/2014, 11:20

... system G p(s)is originally stable For such cases, the PFC can be designed according to the following procedure Step 1: Introduce a PFC as shown in Figure 3. Step 2: Consider designing a PFC G PFC(z)so ... control input will be required (Minami et al., 2010) The overall block diagram of the augmented system for the system with an internal model filter G I M(s)can be shown as in Fig 2 Thus, introducing ... (80) For the obtained ASPR augmented subsystems with PFCs, the adaptive PID controllers are designed as in (53) with parameter adjusting laws given in (71) The designed parameters in (71) are given...

Ngày tải lên: 19/06/2014, 12:20

... shorter in order to achieve a phase lag of 45° Instead of infinite balun, we proposed a stripline structure for impedance matching and the support for helical wire Fig 15 c) shows that matching stripline ... stripline is made of shorter part designed to counteract the imaginary part of the antenna input impedance and longer quarterwave part which is used to tune the real component of antenna input ... 23, No 3, (May 1975), pp 392-397 , ISSN 0018-926X Trang 7Klock, P (1963) A study of wave propagation of helices, University of Illinois Antenna Laboratory Technical Report No 68, March 1963...

Ngày tải lên: 19/06/2014, 19:20

... explain the origins of 2.5 2.6 2.7 2.8 2.9 3 3.1 3.2 3.3 3.4 3.5 0 36 72 108 Translation angle Bearing Stiffness Factor [...]... engineering, Vol 52, pp 527- 634 46 Recent Advances in Vibrations ... contact parts: the shaft, the inner ring, the rolling elements, outer ring and the housing. The deformation of each part will influence the Recent Advances in Vibrations Analysis 36 load ... 2003). A particle traces out a path in a space R n :ℛ → ℛ  (3) where R represents time domain, R n represents the space domain and q represents the position of a particle at an instant...

Ngày tải lên: 19/06/2014, 19:20

... determines, in a recurrence manner, sequences of integrand poles and residues in these poles. The edge diffraction field in both media is determined using a pair of Fredholm- type singular integral ... each point x, including the discontinuity point ξ, converges and its sum is equal to Electromagnetic Waves Propagation in Complex Matter 32  1 00 2 x EE E         (37) ... by the surfaces and are inhomogeneous in space. At the same time, the study of the propagation of waves in layered conducting media and, according to (Born, 1970), in thin films is reduced to...

Ngày tải lên: 19/06/2014, 21:20

... field International Chemical Engineering, Vo.30, No.3, 526-534, ISSN 1385-8947 Temkin, S.(1998).Sound propagation in dilute suspensions of rigid particles J Acoust Soc.Am, Vol.103, No.2,838-849, ... Circular Cylinder in Pulsating Flow Int.J.Heat Mass Transfer, Vo.37, No.15, 2203-2210, ISSN 0017-9310 Tal-Figiel, B (1990) Conditions for instability of the liquid-liquid interface in a ultrasoinc ... 1991a) investigated the effect of an acoustic field on the combustion of coal particles in a flame burner by injecting the particles of 20~70 μm into the burning gas stream and by monitoring the...

Ngày tải lên: 19/06/2014, 23:20

... this link length is of the magnitude of ± 1 g/m3 In the case of an 11.05 km link, the uncertainty in evaluating the attenuation is ± 0.01 dB/km, hence the corresponding error in calculating the ... by substituting (35) to (45) By integrating the corresponding infinite seriesterm-by-term and with the help of (Abramovitz & Stegun, 1964, eq (6.5.37)), the ABEP maybe obtained in closed form ... monitoring system Wireless communication, and in particular cellular networks, are widely distributed, operating in real time with minimum supervision, and therefore can be considered as continuous,...

Ngày tải lên: 19/06/2014, 23:20

... enabling again for their individual tuning of their power levels prior injected into the SOAs However, the two control signals are now coinciding in time, whereas an additional CW’ signal at λ3 ... scheme, employing a strong continuous wave (CW) signal at λ1 commonly inserted into both SOA-MZI branches after passing through the input 3 dB coupler A weak data signal at λ2 is inserted into the ... networks The origin of the nonlinearities lies in the SOA gain saturation and in its correlation with the phase of the propagating wave, since the carrier density changes induced by the input signals...

Ngày tải lên: 19/06/2014, 23:20

... imaging to the study of the filtration process In: Stapf S, Han SI (eds) NMR imaging in chemical engineering, Wiley-VCH, Weinheim, pp 250–2 63 24 Stapf S, Han S (2005) NMR imaging in chemical engineering ... It is obtained by integrating (2 .33 ) to Z t r; t/ D G t /r dt (2 .34 ) Á kr: (2 .35 ) In (2 .35 ) the definition of the wave vector Z t kD G t / dt (2 .36 ) has been introduced.10 The measuring system ... and catalyst support in a fixed bed by magnetic resonance imaging Chem Eng Sci 62(18–20, Sp Iss SI): 533 0– 533 4 DOI 10.1016/j.ces.2007. 03. 034 Grosse J, Dietrich B, Martin H, Kind M, Vicente J, Hardy...

Ngày tải lên: 29/05/2014, 18:32

... counselling after the diagnosis 12 In my opinion working according the guideline requires that the content of the preoperative counselling is put in writing 13 In my opinion working according the ... satisfaction Time investment 3. 88 ± 0.52 3. 86 ± 0.48 3. 81 ± 0. 53 3.81 ± 0.68 3. 75 ± 0.86 3. 73 ± 0. 83 3.52 ± 0.81 2.82 ± 0.90 4; 2–5 4; 2–5 4; 2–5 4; 1–5 4; 1–5 4; 1–5 4; 1–5 3; 1–5 SD: standard ... Status of the guideline Time investment* RI 12 8500 (1) 10 2877 (2) 5 832 (3) 0656 (4) 4579 (5) 8002 (6) 57 43 (7) 1716 (8) 8 736 (9) 18 13 (10) 0 234 (11) 8478 (12) 4884 ( 13) 38 83 (14) 134 8 (15) 2989 (16)...

Ngày tải lên: 11/08/2014, 05:21

... counselling after the diagnosis 12 In my opinion working according the guideline requires that the content of the preoperative counselling is put in writing 13 In my opinion working according the ... satisfaction Time investment 3. 88 ± 0.52 3. 86 ± 0.48 3. 81 ± 0. 53 3.81 ± 0.68 3. 75 ± 0.86 3. 73 ± 0. 83 3.52 ± 0.81 2.82 ± 0.90 4; 2–5 4; 2–5 4; 2–5 4; 1–5 4; 1–5 4; 1–5 4; 1–5 3; 1–5 SD: standard ... Status of the guideline Time investment* RI 12 8500 (1) 10 2877 (2) 5 832 (3) 0656 (4) 4579 (5) 8002 (6) 57 43 (7) 1716 (8) 8 736 (9) 18 13 (10) 0 234 (11) 8478 (12) 4884 ( 13) 38 83 (14) 134 8 (15) 2989 (16)...

Ngày tải lên: 11/08/2014, 16:21

... in human Jurkat T -cells grown in stable isotope labeling by amino acids in cell culture (SILAC) medium supplemented with either natural, light 12C6-arginine or heavy 13C6-arginine [50] Arginine ... 18O-labeling [52] was used to characterize in vivo protein processing in Fas-induced apoptotic Jurkat cells [34 ] In this study, 93 cleavage sites in 71 different proteins were characterized in a ... characterize ATP-binding and GTP-binding proteins in cells, we profiled purine-binding proteins on a proteome scale [40] For this purpose, we used 5¢-p-fluorosulfonylbenzoyladenosine (FSBA) (Fig 3B), a known...

Ngày tải lên: 18/02/2014, 16:20

... mechanism. 23 Eom et al24,25 provided the coarse-grained model of folded polymer chain molecules for gaining insight into unfolding mechanism with respect to folding topology, and it was shown that folding ... discarded.24 ,33 Once a constant, discrete strain tensor Δε0 is prescribed to a unit cell containing protein crystal, the displacement vector u due to strain Δε0 for a given position vector r of a protein ... the initial conformation of a protein crystal as the native conformation deposited in protein data bank (PDB) for a given protein crystal in a unit cell Such initial confirmation for a protein...

Ngày tải lên: 22/02/2014, 08:20