in cell nmr in mammalian cells part 1

... of DNA in a single cell cycle Inactivation of CDK1 leads to re-replication of DNA in eukaryotic cells including human cells [7] Targets of the kinase in the regulation of DNA replication include ... Trang 1cycle in mammalian cellsYuki Komamura-Kohno1, Kumiko Karasawa-Shimizu1, Takako Saitoh1, Michio Sato1, Fumio Hanaoka2, Shoji Tanaka1and Yukio Ishimi1,3 1 Mitsubishi Kagaku Institute ... chromosomes The level of staining during phases G2 -0.4 -0.2 0 0.2 0.4 0.6 0.8 1 1.2 1.4 1.6 P-3Ab P-7Ab P-19Ab P-32Ab P-54Ab P-110Ab A450 P-Ser3 P-Thr7 P-Thr19 P-Ser32 P-Ser54 P-Thr110 phosphopeptides...

Ngày tải lên: 16/03/2014, 13:20

... Akt-independent pathway It should also be noted that DIF-1 did not affect the 0.9 1 1.1 1.2 1.3 1.4 1.5 DIF-1 0 200 400 600 800 Time of monitoring (s) 0.8 1 1.2 1.4 1.6 1.8 Tg * 0.9 1 1.1 1.2 1.3 ... THPH 0.9 1 1.1 1.2 1.3 1.4 1.5 Tg 0.9 1 1.1 1.2 1.3 1.4 1.5 1.6 1.7 DIF-3 0 200 400 600 800 n.s. n.s. Fig 6 Effects of DIF analogs on [Ca 2+ ]cin 3T3-L1 fibroblasts (A) Fura-2-loaded 3T3-L1 cells ... (a) DIFs increase [Ca2+]c in some tumor cells [9–12], (b) DIFs activate Akt⁄ protein kinase B in human leukemia K562 cells [13], (c) DIF-1 inactivates STAT3 in gastric cancer cells [15], and...

Ngày tải lên: 23/03/2014, 09:20

... distribution in interphase cells, and in dividing cells they also localize to the microtubules of the mitotic spindle Results Intracellular levels of the HCV-1a core+1 protein in Huh-7 cells are ... ATG initiator A B C in core ORF myc(+1) pHPI-1494 core+1 nt 825 nt pHPI-1507 core+1 nt 825 nt core+1 nt 825 nt pHPI-1507 nt 515 nt 825 core+1 myc(+1) nt 515 nt 825 core+1 myc(+1) pHPI-1580 ... of HCV-1a in cultured cells; a frameshift mechanism within codons 8–11, yielding a protein known as core+1⁄ F, and ⁄ or translation initiation from internal codons in the core+1 ORF, yielding a...

Ngày tải lên: 30/03/2014, 03:20

... reproduction in Research Trypsin-induced proteome alteration during cell subculture in mammalian cells Hsiang-Ling Huang1, Hsiang-Wei Hsing1, Tzu-Chia Lai1, Yi-Wen Chen1, Tian-Ren Lee1, Hsin-Tsu Chan1, ... trypsin-induced differential protein expression in cervical cancer cells It is interesting and important to know whether trypsine-induced protein alterations in MCF-7 cells are reproduc-ible in ... VDAC1 level in Hela cells starting from 8 hr In summary, trypsinization-induced protein alterations in MCF-7 cells Figure 2 2D-DIGE analysis of the trypsin-induced differentially expressed proteins...

Ngày tải lên: 10/08/2014, 05:21

... competent for autoprocessing in mammalian cells Darunavir and Indinavir inhibit fusion precursor autoprocessing in transfected 293T cells To further examine autoprocessing specificity, we next ... the indicated pEBG construct were incubated with or without protease inhibitors at increasing concentrations Darunavir: 0.1 μM, 1 μM and 10 μM; Indinavir: 1 μM, 10 μM and 100 μM Post-nuclear cell ... significantly less infectious [15-18] Because HIV protease plays a critical role in viral infec-tivity, protease inhibitors targeting the catalytic site have been routinely used in combination with inhibitors...

Ngày tải lên: 10/08/2014, 05:21

... killing following binding (recently reviewed in [1]) Whilst a variety of cell types including plant cells, fungal cells and even bacteria [2] have been described as hosts, mammalian cells are the ... 7A-cotransfected cells, thus demonstrating that strong in vivo binding of the destabilized 13R4-d1-EGFP pro-teins allows them to be maintained in the cells To rule out the possibility that the EGFP part ... 1 April 2005, accepted 7 April 2005) doi:10.1111/j.1742-4658.2005.04709.x The ectopic expression of antibody fragments inside mammalian cells (intrabodies) is a challenging approach for probing...

Ngày tải lên: 19/02/2014, 18:20

... 0 5 0 0 0 5 0.1 1 5 10 50 0 0.1 1 5 10 50 0 0.1 1 5 10 50 0 0.1 1 5 10 50 3 T 3 1 V C O C 3 2 5 1 2 2 9 1 5 6 ) 3 . 8 ( ( 9 3 ) ( 2 8 ) ( 5 ) Fig 3 NIH ⁄ 3T3 cells showed high induction levels ... VP16(AD):GAL4(DBD)(1–147):EcR(C-DEF) [VG(147)E(CVP16(AD):GAL4(DBD)(1–147):EcR(C-DEF)] or VP16(AD):GAL4(DBD)(1–147):EcR(VP16(AD):GAL4(DBD)(1–147):EcR(C-DEF) [VG(147)E(VP16(AD):GAL4(DBD)(1–147):EcR(C-DEF)] ... (V:Hs-LmR-10 or VGEtvy + VHs(1–11)-LmR (12) (V:Hs-LmR-11) or VGEtvy + VP16:LmRXR (VLmR) and pFRLuc were transfected into 3T3 cells using superfect lipid reagent The transfected cells were grown in the...

Ngày tải lên: 16/03/2014, 12:20

... repeats causing the propagation of the optical discharge 101112131415 Points are experimental data and the line corresponds to the data linear fit (slope=1.38±0.06 µm W-1, intercept=10.1±0.2 µm, ... correspond to the data linear fit (slope=0.110±0.002 m s-1 W-1, intercept= 0.190±0.004 m s-1, correlation coefficient > 0.993) 0.02.55.07.510.0 Trang 19Thermal Effects in Optical Fibres 7 The ... Conduction in Nonlinear Media 667 Michael M Tilleman Trang 11on electrical discharge machining, mixing convection are included in this book aiming The experimental and theoretical investigations,...

Ngày tải lên: 19/06/2014, 10:20

... Mechanics, Tsinghua University, Beijing, China 1 Introduction Excellent physical and chemical properties make silicon carbide (SiC) a prominent candidate for a variety of applications, including high-temperature, ... mechanisms distinct from their crystalline counterparts The coexistence of brittle grains and soft amorphous grain boundaries (GBs) consisting in nanocrystalline SiC (nc-SiC) results in unusual deformation ... First published September, 2011 Printed in Croatia A free online edition of this book is available at www.intechopen.com Additional hard copies can be obtained from orders@intechweb.org Silicon Carbide...

Ngày tải lên: 19/06/2014, 11:20

... published August, 2011 Printed in Croatia A free online edition of this book is available at www.intechopen.com Additional hard copies can be obtained from orders@intechweb.org Advances in PID Control, ... where the Trang 11introduced tuning strategies are based on mixed H2/H-infty, multi-objective genetic algorithm, fuzzy logic, and particle swarm optimization techniques In Chapter 10 the digitally ... poles and one zero (Franklin et al., 2010): T(s) = (s/aζω n) +1 s2/ω2 Trang 17Phase Systems 3normalising effect and also a time normalising effect in the corresponding step response Therefore...

Ngày tải lên: 19/06/2014, 12:20

... published July, 2011 Printed in Croatia A free online edition of this book is available at www.intechopen.com Additional hard copies can be obtained from orders@intechweb.org Advances in Satellite ... 3free online editions of InTech Books and Journals can be found at www.intechopen.com Trang 5Contents Preface IX Part 1 Antennas in Satellite Communications 1 Chapter 1 Helical Antennas in Satellite ... Trang 13Part 1 Antennas in Satellite Communications Trang 151 Helical Antennas in Satellite Radio Channel Maja Škiljo and Zoran Blažević University of Split, Faculty of electrical engineering,...

Ngày tải lên: 19/06/2014, 19:20

... August, 2011 Printed in Croatia A free online edition of this book is available at www.intechopen.com Additional hard copies can be obtained from orders@intechweb.org Recent Advances in Vibrations ... beams including waves of both propagating and near-field types By the phase-closure principle, also referred to as the wave-train closure principle (Cremer et al., 1973), Mead (1994) determined ... accessible to wider audience Dr Natalie Baddour Department of Mechanical Engineering University of Ottawa Canada Trang 13Part 1 Analytical Methods Trang 151 Exact Transfer Function Analysis of Distributed...

Ngày tải lên: 19/06/2014, 19:20

... García-Collado, G.J Molina-Cuberos, J Margineda and J Represa Chapter 5 Electromagnetic Waves in Contaminated Soils 117 Arvin Farid, Akram N Alshawabkeh and Carey M Rappaport Part 2 Extended Einstein’s Field ... First published June, 2011 Printed in Croatia A free online edition of this book is available at www.intechopen.com Additional hard copies can be obtained from orders@intechweb.org Electromagnetic ... and  scattering  in  soil  by  solving  Maxwell’s  equa‐ tions using a finite difference time domain (FDTD) model. The chapter explains how  the  lossy  and  dispersive  soil  medium  (in  both ...

Ngày tải lên: 19/06/2014, 21:20

... Extreme Winds in Kuwait Including the Effect of Climate Change 89 S Neelamani and Layla Al-Awadi of Wind Power Conversion 113 Chapter 6 Efficient Modelling of Wind Turbine Foundations 115 Lars ... conservation using Bernoulli equation between station 1 and 2, then 3 and 4, Eqn 5 and Eqn 6 can be obtained respectively 12 1 Trang 17Aerodynamics of Wind Turbines 5 12 (9) Substituting equation 7 into ... Associate Professor Civil and Environmental Engineering University of Windsor Trang 13Part 1 Aerodynamics and Environmental Loading of Wind Turbines Trang 152 Aerodynamics of HAWTs The majority of...

Ngày tải lên: 19/06/2014, 21:20

... 9.0 x 10 -10 Sucrose 5.2 ± 0.2 x 10 -10 ** 5.2 x 10 -10 Glycine 1. 2 ± 0 .1 x 10 -9 ** 1. 1 x 10 -9 Urea 1. 4 x 10 -9 * *1. 4 x 10 -9 Tryptophan 5.6 x 10 -10 ***5.7 x 10 -10 *(Yang... ... sucrose 1. 5 P [-] 1. 4 1. 3 1. 2 1. 1 1. 0 0 10 30 60 Angle [ deg ] Fig 15 ... micro-channels Int J Heat and Mass Transfer, 48 (10 ), 19 82 -19 98, ISSN 0 017 -9 310 Higbie, R (19 35) ... Chapter 10 Chapter 11 Chapter 12 Chapter 13 Part 3 Chapter 14 Chapter 15 Chapter 16 Part 4 Chapter 17 Chapter 18 Contents VII Combined Heat and Mass Transfer 423 Successive Linearisation Solution...

Ngày tải lên: 19/06/2014, 23:20

... 10 3 10 3 10 3 10 3 10 6 10 7 10 7 10 8 11 0 11 1 11 1 11 2 11 5 11 5 11 5 11 6 12 2 12 5 12 5 12 5 12 8 12 8 12 8 12 9 13 4 13 5 13 5 13 6 13 9 14 0 14 0 14 0 14 3 14 4 14 4 14 4 15 1 15 1 15 1 15 2 15 6 15 7 15 9 Contents ... 18 5 18 5 18 9 19 0 19 1 19 1 19 4 19 5 19 6 19 9 2 01 2 01 202 Imaging with an Inhomogeneous Gradient 203 Reference ... vector for spin density Value Meaning 1: 055 10 34 J s 1: 3 81 10 23 J=K 6:022 10 23 mol 2:675 10 8 rad s T 0:673 10 8 rad s T 2: 516 10 8 rad s T 1: 084 10 8 rad s T 10 H=m Planck constant Boltzmann constant...

Ngày tải lên: 29/05/2014, 18:32

... guideline Time investment* RI 12 8500 (1) 10 2877 (2) 5832 (3) 0656 (4) 4579 (5) 8002 (6) 5743 (7) 17 16 (8) 8736 (9) 18 13 (10 ) 0234 (11 ) 8478 (12 ) 4884 (13 ) 3883 (14 ) 13 48 (15 ) 2989 (16 ) 02 71 (17 ) ... Res 19 84, 11 :14 8 -15 5 Page 11 of 12 (page number not for citation purposes) Implementation Science 2009, 4 :10 12 13 14 15 16 17 18 19 20 21 http://www.implementationscience.com/content/4 /1/ 10 Oppewal ... writing 13 In my opinion working according the guideline requires the availability of written information that supports the oral counselling at discharge 14 In my opinion working according the...

Ngày tải lên: 11/08/2014, 05:21

... guideline Time investment* RI 12 8500 (1) 10 2877 (2) 5832 (3) 0656 (4) 4579 (5) 8002 (6) 5743 (7) 17 16 (8) 8736 (9) 18 13 (10 ) 0234 (11 ) 8478 (12 ) 4884 (13 ) 3883 (14 ) 13 48 (15 ) 2989 (16 ) 02 71 (17 ) ... Res 19 84, 11 :14 8 -15 5 Page 11 of 12 (page number not for citation purposes) Implementation Science 2009, 4 :10 12 13 14 15 16 17 18 19 20 21 http://www.implementationscience.com/content/4 /1/ 10 Oppewal ... writing 13 In my opinion working according the guideline requires the availability of written information that supports the oral counselling at discharge 14 In my opinion working according the...

Ngày tải lên: 11/08/2014, 16:21

... TEAPLNPKANR (10 6 -11 6) and constitutes a neo-N-terminus indicative of HIV-1mediated protein processing Processing of b-actin by the HIV -1 protease between Leu105 and Thr106 was already identified in previous ... medium 13 C -arginine human Jurkat T -cells SILAC medium 12 C -arginine freeze-thaw lysate recombinant HIV -1 protease control C 947 948 949 950 m/z 650 D 6 51 652.37 6 51. 88 6 51. 39 947.49 947. 71 948.06 ... is illustrated in Fig In an ongoing project, host cell substrates of the HIV -1 protease are catalogued in human Jurkat T -cells grown in stable isotope labeling by amino acids in cell culture (SILAC)...

Ngày tải lên: 18/02/2014, 16:20

... coarse-grained model of folded polymer chain molecules for gaining insight into unfolding mechanism with respect to folding topology, and it was shown that folding topology plays a role on the protein ... E.; Florin, E.-L Proc Natl Acad Sci USA 2006, 10 3, 10 248 12 Wilhelm, J.; Frey, E Phys Rev Lett 19 96, 77, 25 81 13 Bustamante, C.; Marko, J F.; Siggia, E D.; Smith, S Science 19 94, 265, 15 99 14 Bustamante, ... proteins such as protein unfolding mechanics Such protein unfolding experiments has been illuminated in that these studies may provide the free energy landscape of proteins related to protein folding...

Ngày tải lên: 22/02/2014, 08:20