important but not for egq

... these AtxA-binding proteins, but were not toxic Our results show that F24 and the last 26 amino acid residues of potently neurotoxic AtxA are not enough to transform a nontoxic sPLA2 into a neurotoxic ... ionization mass spectroscopy, 13.904 kDa for AtnI2/AtxA(K108N) and 13.937 kDa for AtnI2(N24F)/AtxA(K108N), perfectly match their theoretical values, assuming formation of all seven disulfide bonds ... residues differing from the corresponding AtnI2 region distributed mainly on the molecular surface (Fig 2), did not induce any substantial conformational changes in the protein fold In contrast to...

Ngày tải lên: 31/03/2014, 08:20

... Glycosylation of BST-2 is not required for inhibition of virus release and for sensitivity to Vpu Glycosylation of BST-2 is not required for inhibition of virus release and for sensitivity to Vpu ... only the post-ER form of BST-2 can efficiently homo-dimerize while the high mannose form remains largely, if not exclusively, in monomeric form BST-2 dimerization is important for inhibition of ... as wt BST-2 and did not inhibit release of wt NL4-3 (Fig 4B) We therefore conclude that glycosylation of BST-2 is not critical for the inhibition of virus release and does not affect sensitivity...

Ngày tải lên: 12/08/2014, 23:22

... task (button) Further from Fig 2, mean DTC was higher in elderly than in younger subjects for some but not for other task combinations, thus reflecting agerelated deficits of dual-task performance ... summarized in the top part of Tab DTC was small for both constituent tasks of walko+memo (i.e., for walking as well as for memorizing), but was large for both constituent tasks of walko+checkgw Elderly ... seniors on the registered task may not reflect ARD, but rather seniors' higher priority for the non-registered task [26] Other authors avoided this design flaw, but yielded discrepant results: some...

Ngày tải lên: 19/06/2014, 08:20

... Staining of leaf sections with MitoTracker was not successful, therefore double labelling was performed on protoplasts Page of 14 (page number not for citation purposes) BMC Plant Biology 2008, ... http://www.biomedcentral.com/1471-2229/8/40 Ornithine-δ-aminotransferase does not contribute to stress-induced proline accumulation The mitochondrial localisation of δOAT indicated that it is not involved in the formation of Pro, since ... homozygous for the oat3 T-DNA insertion but heterozygous for the δOAT-GFP construct In bacteria and mammals, transamination of Orn constitutes an alternative route for GSA/P5C and subsequently Pro formation...

Ngày tải lên: 12/08/2014, 05:20

... in a scientific culture that only values us for what we’ve done lately, not for what we once did That’s the worst, and best, thing about it But not for a few, and Crick clearly was one of those ... that rare but because few are fortunate enough to make a discovery that, quite literally, changes the world He did, and for that reason he would be on most people’s list of the five most important ... contributions for granted, and they should Their focus is not on our past, it’s on their present and future, their ideas, their problems The Francis Cricks of the world are pretty rare - not because...

Ngày tải lên: 14/08/2014, 14:21

... possible that the reduced performance of yearlings in pairs is due to a reduced optimal reproductive effort for these birds These hypotheses are not mutually exclusive, but each is considered in ... of the male was years (range 1–10) for yearling-female pairs, years (2 –11) for yearling-female groups, years (2–11) for older-female pairs and years (1–11) for older-female groups; Kruskal–Wallis, ... reproductive effort Analyses of breeder quality (Results) show that the difference is not due to differences in female quality, another general explanation of age effects in birds (Forslund & Pärt...

Ngày tải lên: 14/03/2014, 16:20

... set, is therefore another important parameter of data quality The above numerical criteria are usually quoted for all data and for the highest resolution shell Unfortunately, it is not customary ... collected for phasing For refinement, it is most important to collect a complete data set to the resolution limit of Protein crystallography for non-crystallographers diffraction, whereas for phasing ... unambiguous way Unfortunately, PDB files not contain any information about the procedures that were used for metal assignment and refinement, and even the relevant papers often relegate this information...

Ngày tải lên: 16/03/2014, 06:20

... used for Agrobacterium-mediated transformation of the potato line K41 We obtained 65 and 58 independent transgenic lines for the Rar1 and Sgt1 genes, respectively Page of (page number not for ... (PT29), non-transformed K41 lines and K41 lines that were transformed with the construct containing either Rar1 or Sgt1, but not silenced The inoculation and resistance evaluation were performed as ... resistance phenotype http://www.biomedcentral.com/1471-2229/8/8 as the RB gene In addition, the role the Rar1 and Sgt1 genes are not universal and these genes are not essential for resistance...

Ngày tải lên: 12/08/2014, 05:20

... and HLT ex vivo, but not in macrophages that express higher levels of tetherin In sum, our data suggest that the ability of Vpu to counteract tetherin is an important determinant for HIV-1 cell ... shown that Vpu is phosphorylated by CK-II [12], but the importance of an active CK-II for the ability of Vpu to degrade tetherin is not known Therefore, we measured Vpu-mediated tetherin degradation ... of p24 (1 ng p24) [25] showed that only changes in nef, but not in vpu, impaired viral infectivity (Fig 3C) Most Figure Vpu S52A does not impair HIV-1 release from P4-CCR5 cells (A) Western blot...

Ngày tải lên: 12/08/2014, 23:22

... binding were not important for binding to podoplanin Podoplanin contains sialylated O-glycans [54], and we next analyzed if glycosylation of podoplanin is essential for binding to CLEC-2 For this, ... podoplanin-specific antibody 18H5, but not antibodies with other specificities recognize non-viable cells (A) CEM×174 cells were analyzed for their distribution in the forward and sideward scatter, ... interaction was not inhibited by mannan and was not enhanced by expression of the viral Env protein In agreement with these results, soluble HIV-1 Env protein bound specifically to DC-SIGN but not to...

Ngày tải lên: 12/08/2014, 23:23

... the distributions of two polymorphism’s genotypes in the controls were both consistent with Hardy-Weinberg equilibrium except for one study [26 ]for p53 Arg72Pro, and one studies [21] for GSTP1 ... controls) for p53 Arg72Pro and studies (1885 cases and 2194 controls) for GSTP1 Ile105Val were available for this meta-analysis The minor Pro allele (for p53 Arg72Pro) and Val allele (for GSTP1 ... Arg75Pro for the Pro/Arg+Pro/Pro genotypes, compared with the Arg/Arg genotype Figure ORs (log scale) of esophageal cancer associated with GSTP1 Ile106Val for the Ile/Val+Val/Val genotypes, compared...

Ngày tải lên: 25/10/2012, 11:40

... difference for the platelet counts for those achieving CR and those who did not (982 x 109/L for both groups) The drop of the platelet count over the months was 50.3 % for all 45 MPD patients, 56.6 % for ... with PR+F (not shown) Reliable methods for the measurement of anagrelide metabolites were not available at the time of study Effects on TPO and sIL-6R levels TPO PV and ET patients did not differ ... significant changes were observed for this variable over the studied months, neither for all patients, nor for those entering CR or PR+F (Figure 1) The mean ± 2SD values for our patients at start were...

Ngày tải lên: 03/11/2012, 10:52

... am not overambitious I am happy with my work I am not famous but I have independence I cannot create some of the more daring clothes that I would perhaps have been able to create in Europe but ... mistress He even wanted to buy me jewelry but I did not accept That's why I am not wearing any.” “They would add nothing to your appearance Nobody would notice them,” I said “That's sweet Don't ... business There was no other viable option for me in Egypt I had a few days‟ grace before starting work I went for a long stroll in the city I was away from Cairo for only a few months and yet it was...

Ngày tải lên: 06/11/2012, 17:33

... which have not been investigated, and we therefore cannot exclude the possibility that other prolines in tau may be important for its binding to Fyn-SH3 Here, we have demonstrated, for the first ... Prescott ED, Fuhrer C & Burden SJ (2001) Src, Fyn, and Yes are not required for neuromuscular synapse formation but are necessary for stabilization of agrin-induced clusters of acetylcholine receptors ... interact, but to differing extents, with Fyn-SH3 This suggests that the results shown here are not artefactual, owing to incorrect folding of the tau mutants, at least not in the regions responsible for...

Ngày tải lên: 14/02/2014, 14:20

... results not only show that the regulatory role for PI3-K in P2Y12 signaling is conserved in mouse and human platelets, but also highlight the importance of the PI3-Kb isoform PI3-Kb and not PI3-Kc ... together, these results suggest that, in both mouse and human platelets, the PI3-Kb but not the PI3-Kc isoform contributes to platelet procoagulant activity following P2Y12 stimulation Discussion ... platelets Knowing that the PI3-Kß isoform is responsible for a considerable amount of the PIP3 formed in platelets [21], this may suggest that the PIP3 produced by this isoform leads to plasma membrane...

Ngày tải lên: 18/02/2014, 16:20

... it is apparently not used for ubiquitin addition Therefore, the reason why Lys147 rather than Lys5 is the main acceptor site for ubiquitin in Rac1 remains to be elucidated Of note, however, is ... which may not be fulfilled by Rac1b pull-down C Lys147 The main target site for Rac1 ubiquitination is Lys147, a surface residue not hampered in Rac1b We first reasoned that the site(s) for ubiquitin ... not involved in contacts with any of the interacting proteins described so far, and is not affected by the 19-residue insertion in Rac1b Therefore, the impairment of Rac1b ubiquitination is not...

Ngày tải lên: 18/02/2014, 16:20

... and not- for- profit governance.) The 20 Questions for Not- for- Profits Series 20 Questions Directors of Not- for- profit Organizations Should Ask about Governance 20 Questions Directors of Not- for- profit ... agencies often ask for quantitative information in their grant application forms Qualitative measures deal with opinions and feelings — very important considerations for not- for- profits — and ... inconsistent performance Staff can be employed as the primary human resource of a not- forprofit or to perform specific tasks that volunteers are less equipped to perform The employees of not- for- profit...

Ngày tải lên: 21/02/2014, 17:20

... focimatrix (for focal intra-epithelial matrix) [7] These observations indicate that ECM components contribute to the microenvironment of GC, but their specific roles in follicular development have not ... isolation of GC), or prepared for western immunoblotting For studies of ERK1/2 phosporylation, GC were cultured on LN or plastic, with and without inhibitors, for 24 h before western immunoblotting ... for autoradiography from Integra Bioscience (Cergy-Pontoise, France) For protein assay, the BC Assay protein kit was obtained from Uptima Interchim (Montluçon, France) Immobilon P membranes for...

Ngày tải lên: 05/03/2014, 17:20

... also usually different for not- for- profit corporations, although this What is a not- for- profit corporation? varies from jurisdiction to jurisdiction Incorporating a Not- for- profit corporations ... organization Corporations & Directors Ⅵ |1| The not- for- profit board of directors For- profit and not- for- profit directors share a legal The not- for- profit corporation is governed by a board responsibility ... Canadian essential information throughout the text on treatment Bar Association Charity and Not- for- Profit Law Section, of not- for- profit corporations and their directors under for their input and...

Ngày tải lên: 08/03/2014, 16:20

... Accounting Most of not- for- profit accounting is no different from for- profit accounting The primary area which is different for not- for- profit organizations is accounting for contributions For- profit ... information on taxes for not- for- profit organizations Relevant information from former Chapter 29, “Principal Federal Tax Forms Filed,” has now been included in new Chapter 28 The actual tax forms ... Model for Public Colleges and Universities and Other Not- for- Profit Organizations Reporting under GASB” (now Chapter 15), and • “E-Business for Not- for- Profit Organizations: How Can Not- for- Profits...

Ngày tải lên: 13/03/2014, 23:41