immunocytochemical analysis of human pluripotent stem cells

... embryonic stem cells (CESCs) closely resemble the morphology of human pluripotent stem cells (a) Colonies of CESCs grown on irradiated feeder cells (4x magnification, left) and morphology of individual ... both human and marmoset pluripotent stem cells carrying the en-hanced green fluorescent protein (eGFP) reporter 2 Materials and Methods 2.1 Derivation of Human Induced Pluripotent Stem Cells ... markers of committed neural cells, were first observed two weeks after replating We compared the propensity of neural and retinal lineage cells, human pluripotent stem cells gave rise to cells

Ngày tải lên: 01/11/2022, 09:48

... developed a defined culture system to derive human foregut stem cells (hFSCs) from hPSCs These stem cells can self-renew in vitro and resemble multipotent cells of the anterior primitive gut ... Foregut Stem Cells derived from hPSCs (B) Bright-field image of foregut stem cells growing as an epithelium (C and D) After ten passages, approximately 80% of hFSCs express Ki67 (E) CGH profile of ... for a Prolonged Period of Time in Defined Culture Medium We then tested the capacity of foregut SOX2+cells to self-renew in vitro DE cells derived from human embryonic stem cells (hESCs) were differentiated

Ngày tải lên: 02/11/2022, 10:44

... embryonic stem cells Stem Cells 26, 455–64 (2008). 3 Armstrong, L et al Human induced pluripotent stem cell lines show stress defense mechanisms and mitochondrial regulation similar to those of human ... embryonic and induced pluripotent stem cells Pluripotent stem cells (PSCs) are reported to have low levels of reactive oxygen species (ROS), and the reduced environment seen in these cells has been ... Prepared according to manufacturer’s instructions (05850) Human embryonic stem cells (HuES-7; HuES-9) and human induced pluripotent stem cells (adult dermal fibroblast iPS; neonatal foreskin fibroblast

Ngày tải lên: 04/12/2022, 10:32

... plating the cells, as previously described[6,7] Cultures of primary human fetal hepatocytes, human normal fetal liver-CD34+ cells, and HepaRG® cells which are terminally differentiated hepatic cells ... stages of liver stem cells which have the capacity for unlimited proliferation and mutilineage differentiation, and such kind of liver stem cells change their expression markers in dependence of di ... Hepatic progenitor cells Hepatic differentiation Hepatoblasts Hepatocytes Human pluripotent stem cells Serum-free culture A B S T R A C T Limited growth potential, narrow ranges of sources, and difference

Ngày tải lên: 04/12/2022, 15:13

... and toxicity of O6 -methylguanine, and of MGMT, responsible for the removal of the O6-methyl adduct In fact, the lack of ex-pression of one of MMR components and/or the presence of high MGMT ... densities (O D.) of the protein of interest and those of tubulin The results are representative of one out of two experiments with similar results SJ: SJGBM-2 Panel C Analysis of MGMT activity ... activity was evaluated as fmol of32P-NAD+/μg of protein MGMT activity assay and bisulfite sequencing analysis of MGMT promoter methylation Cells (1×106) were lysed in 0.5 ml of a buffer containing 0.5%

Ngày tải lên: 05/11/2020, 01:28

... human stem cells cannot automatically be considered to behave in a similar way to other adherent cells lines This is because with human stem cells it is the cell itself that forms the basis of ... 10.6 cells/bead; b day 2, 10.7 cells/bead; c day 3, 6.2 cells/bead; d day 4, 9.5 cells/bead; e day 5, 19.7 cells/bead; f day 7, 52.1 cells/bead; g day 8, 67.6 cells/bead; h day 9, 98.4 cells/bead; ... of human stem cells is not a trivial exercise Indeed, expanding human stem cells in culture is currently more of an art than a science so much so that some laboratories cannot or do not grow human

Ngày tải lên: 02/11/2022, 10:41

... haematopoietic stem cells Nodal Member of the TGF-beta superfamily Anterior-posterior and visceral endodermal patterning Gfap Component of intermediate filaments of glial cells of the astrocyte ... sample of cells A frequently discussed matter in ultrasound trapping is the viability of trapped cells after being exposed to ultrasound.Nyborg (2001)reviewed the 80-year history of studies of biologic ... acquisition and processing software (Soft Imaging System, SIS, GmbH) Experimental procedure Single cell suspensions of ES cells were prepared as described above and diluted to 3000 cells/mL The ultra-sound

Ngày tải lên: 02/11/2022, 10:47

... hPSCs. pluripotent stem cells, NANOG, pluripotency INTRODUCTION Human pluripotent stem cells (hPSCs), including human embryonic stem cells (hESCs) and human induced pluripo-tent stem cells (hiPSCs), ... co-transfected FACS analysis showed that nearly 17% of cells in the dCas9-VPR group turned on BFP, while 24.7% of cells in the rtTA group were BFP posi-tive after Dox induction, and only 0.6% of cells exhibited ... panel,flow cytometry analysis of GFP+cells in each group (C) Q-PCR analysis of NANOG expression 2 days after transfection; the dCas9-VPR system showed nearly 150-fold up-regulation of NANOG mRNA Relative

Ngày tải lên: 19/11/2022, 11:38

... well as physiology of a human CM and therefore have limitations in predicting drug responses on the human heart Human pluripotent stem cell-derived CMs (hPSC-CMs) offer a human-based, physiologically ... cardiac differentiation of hPSCs result in heterogeneous pools of CMs consisting predominantly of ventricular-like cells with a small percentage of atrial-like and nodal-like cells (Blazeskiet al, ... (Fig 1B) Flow cytometry analysis of GFP expression at day 15 revealed a decrease in the proportion of NKX2.5-expressing cells upon treatment with RA Sixty-five percent of all cells expressed GFP

Ngày tải lên: 19/11/2022, 11:40

... differentiation of human adipose stem cells into functional hepatocytes Jieqiong Huang*, Xinyue Guo*, Weihong Li & Haiyan Zhang The generation of hepatocytes that are derived from human adipose stem cells ... presented in the cells of four groups The percentage of cells with LDL partiparti-cles in cells of Chir99021 at day 20 was relatively higher than other groups, which resembles the human hepatocytes ... expression of FOXA2 and CXCR4 in cells was examined by flow cytomet-ric analysis The data showed that the percentage of FOXA2 positive cells in four treated group was 78.8% (activin A-treated cells),

Ngày tải lên: 19/11/2022, 11:45

... number of CDCs for experiments, regardless of age To characterize the phenotype of CDCs, the percentage of cells positive for CD90 and CD105, which are validated markers of mesenchymal stem cells14, ... the function of cardiac stem cells In addition, these factors do not necessarily influ-ence the quantity and quality of stem cells uniformly For example, although hematopoietic stem cells from ... determinant of the quantity and quality of CDCs Senescent cells accumulate in tissues with advancing age Tissue-specific stem cells, for example those of the hematopoietic and musculoskeletal system

Ngày tải lên: 04/12/2022, 14:53

... and self-renewal of multipotent human intestinal stem cells (a) Expansion of single human duodenum organoid cells (passage 7) able to form new organoids (n¼ 3, mean±s.e.m.) (b) Human duodenum organoids ... expansion of duodenum stem cells (Fig 4b) This shows that simple addition of liposomes to organoid medium enhances the stability of Wnt3a protein and allows the serum-free expansion of human organoids ... expansion of undifferentiated ESCs (Fig 2d), and higher proliferation of human duodenum organoid cells than purified Wnt3a (Fig 2e) To specifically assay for the expansion of organ stem cells, we

Ngày tải lên: 04/12/2022, 15:06

... culture, expansion and manipulation of human pluripotent stem cells The culmination of two events has enabled the production of human embryonic stem cells: the birth of the first IVF test-tube baby ... summarizing 12 years of our team’s experience, skill and knowledge in the derivation, culture and expansion of human embryonic stem cells, and more recently, of human induced pluripotent stem cells The ... translational research in the field of embryonic and induced pluripotent stem cells It commences with practical aspects of the derivation and growth of human embryonic stem cells from inner cell mass...

Ngày tải lên: 22/03/2014, 09:20

... Figure The combination of stem cell models and epigenomics in studies of the role of non-coding mutations in human disease Epigenomic analyses of cells derived through in vitro stem cell differentiation ... embryonic stem cell; H3K4me1, methylation of lysine of histone H3; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of lysine ... stem cell; hESC, human embryonic stem cell; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of lysine 27 of histone H3;...

Ngày tải lên: 11/08/2014, 12:21

... 13 The expression of miR-92 has been reported in human embryonic stem (ES) cells [16,26], mouse ES cells[ 20] or Page 12 of 17 (page number not for citation purposes) Journal of Translational Medicine ... The tested adult cells were Human Microvascular Endothelial Cells (HMVEC), Human Umbilical Vein Endothelial Cells (HUVEC), Umbilical Artery Smooth Muscle Cells (UASMC), Normal human astrocytes ... undifferentiated and differentiated human embryonic stem cells Stem Cells Dev 2007, 16(6):1003-1016 Page 15 of 17 (page number not for citation purposes) Journal of Translational Medicine 2009, 7:20...

Ngày tải lên: 18/06/2014, 15:20

... isolation of relatively pure populations of these cells for in vitro study of the cellular processes in thalassemic stem cells While previous attempts have focused on the cell culture system, in ... The isolated HSCs/CD34+ cells were harvested for phosphoproteomic analysis and cultivation of erythroid cells The cultivation of erythroid cells derived from HSCs/CD34+ cells was performed according ... vitro culture is capable of sustaining a large number of cells, which can confer specific advantages and disadvantages The use of freshly isolated human hematopoietic stem cells from thalassemic...

Ngày tải lên: 18/06/2014, 19:20

... differentiation of cultured human mesenchymal stem cells from marrow Tissue Eng 1998, 4:415-428 35 Mueller MB, Tuan RS: Functional characterization of hypertrophy in chondrogenesis of human mesenchymal stem ... mesenchymal stem cells for osteoarthritis therapy Nat Clin Pract Rheumatol 2008, 4:371-380 Estes BT, Wu AW, Guilak F: Potent induction of chondrocytic differentiation of human adipose-derived adult stem ... arcades of Benninghoff [29] and by the appearance of hypertrophic cells Analyses of lacunae surface areas in the different growth plate zones revealed mean lacunae surface areas ± SD of 100.8...

Ngày tải lên: 09/08/2014, 14:22

... differentiation of size-sieved stem cells into electrically active neural cells Stem Cells 2002, 20(6):522-529 Tzeng SF, Tsai MJ, Hung SC, Cheng H: Neuronal morphological change of size-sieved stem cells ... differentiation of human mesenchymal stem cells into insulin producing cells J Biomed Mater Res A 2008, 86(4):1097-1105 Chao KC, Chao KF, Fu YS, Liu SH: Islet-like clusters derived from mesenchymal stem cells ... Fishman B, Ziskind A, Shulman M, Itskovitz-Eldor J: Differentiation of human embryonic stem cells into insulin-producing clusters Stem Cells 2004, 22(3):265-274 Chang CF, Hsu KH, Chiou SH, Ho LL, Fu...

Ngày tải lên: 10/08/2014, 05:21

... H, Baker SC, et al.: Genome wide profiling of human embryonic stem cells (hESCs), their derivatives and embryonal carcinoma cells to develop base profiles of U.S Federal government approved hESC ... hematopoietic differentiation of human embryonic stem cells Blood 2003, 102:906-915 Woll PS, Martin CH, Miller JS, Kaufman DS: Human embryonic stem cell-derived NK cells acquire functional receptors ... the presence of CD34+/ KDR+ bipotential cells in non -human (Cynomolgus) ESCs Kennedy et al [20] recently reported the generation of BLCFCs from hESCs However, the rarity of the cells with hemangioblast...

Ngày tải lên: 14/08/2014, 08:20