identification of stem cell specific genes

... features of stem cells Since stem cell renewal and differentiation take place at early stages of embryogenesis, the early developing embryo is an ideal system for the in vivo analysis of stem cells ... undifferentiated status of self-renewing stem cells Stem cells show a hierarchy of developmental potency in mouse (Fig 1-1) Totipotent stem cells include the zygote and the 8-cell morula that have ... model system will provide valuable insights into also mammalian systems Trang 15Chapter I Introduction Chapter I Introduction 1.1 Stem cells 1.1.1 Developmental potency of stem cells Stem cells...

Ngày tải lên: 14/09/2015, 13:38

... reprogramming genes (Supplementary Fig S8a) The upregulated genes in the treated MEFs included not only germ cell-specific genes, but also tissue-specific genes such as nervous system- and immune system-specific ... including germ cell-specific genes, nervous system-specific genes and immune system-specific genes were selectively upregulated, but about two third of genes including somatic genes such as Hoxa1 and ... sensitivity to detect mRNA of the endogenous Vasa These results suggested that both the expression levels of germ cell-specific genes and the number of upregulated germ cell-specific genes were not high...

Ngày tải lên: 04/12/2022, 16:33

... regulation of distinct physio-pathological processes including development of normal stem cells and carcinogenesis In this study we aimed to investigate microRNA profile of cancer stem-like cells ... subpopulation of tumor cells exhibits stem cell-like features [7] This reservoir of stem-like cells within the bulk tumor is considered as tumor-initiating or cancer stem-like cells (CSLCs) with ... 3’UTR of several oncogenes were pre-dicted to be targeted by miR-26b (202 out of 348 oncogenes), 200c (161 out of 348 oncogenes), miR-203 (216 out of 348 oncogenes), and miR-363-3p (175 out of 348...

Ngày tải lên: 20/09/2020, 18:26

... are similar to those of humans [6] And it offers the possibility of in-depth study of the transcription levels of SATs, but this is difficult in humans At present, most of the studies mainly ... further identify key sex-specific path-ways and genes in the SATs of pigs These findings may contribute to further understanding of the functions of porcine ATs and the mechanisms of regulating fat ... levels of ECM-related genes in different adipose tissues from bulls, cows and steers of Korean cattle (Hanwoo), and found that the expressions of  ECM-related genes  in the omental adipose tissue of...

Ngày tải lên: 30/01/2023, 20:44

... used Neural stem cells have been isolated from various areas of the adult brain and spinal cord Foetal stem cells A relatively new stem cell type belongs to the group of foetal stem cells (FSC) ... Limited lifespan (population doublings) Production of endless number of cells Production of endless number of cells Production of limited number of cells Chromosome length is maintained across serial ... source of harm [35,37] Examples of risk fac-tors are the type of stem cells used, their procurement and culturing history, the level of manipulation and site of injection Because of the variety of...

Ngày tải lên: 18/06/2014, 19:20

... of PCR of CYP genes, we used the same degenerate forward and a slightly modified reverse primer (see Table 1) from that used in the study of P chrysosporium For the second nested PCR of CYP genes, ... in the biosynthesis of essential compounds like ergosterol, which is a constituent of fungal cell membranes, and in the detoxification and biodegradation of a broad spectrum of environmental chemical ... used for RNA preparation Construction of degenerate primers for cDNA isolation of CYP genes In a previous study to search for unknown CYP genes in cultures of P chrysosporium, a degenerate primer...

Ngày tải lên: 20/06/2014, 20:20

... transplantation of stem cells is to promote the regeneration of tissue specific cells and subsequent morphological and functional recovery of organs of all lineage cells [8,9] Hence, bone marrow stem cells ... HGF-CNP enhanced the differentiation of stem cells into hepatocytes and supported the reversal of fibrolysis of extracellular matrix (ECM) Conclusion: Bone marrow stem cells were isolated, characterized ... which stem cells regenerate the respective parenchymal cells, heading to repair of the organs in vivo is yet to be completely understood Cell fusion, in which stem cells fuse with the somatic cell...

Ngày tải lên: 11/08/2014, 00:23

... some of which could be associated with the pathogenesis of T1D in some susceptible individuals These data expand our knowledge of specific wheat globulins and will enable further elucidation of ... all of the three BAC clones was used as a hybridization probe All of the cultivars examined contained Glo-3 genes Based solely on the number of bands, there are at least two copies of Glo-3 genes ... individual copies of Glo-3 genes. Multiple bands of different intensities were observed on the fluorograph for all of the studied wheat cultivars (Fig-ure 3) Our results indicate the presence of multiple...

Ngày tải lên: 12/08/2014, 03:20

... overall assessment of the importance of histone modifications on stem cell biology To evaluate the importance of histone acetylation on ES cell differentiation, we treated cells with the histone ... revealed the suppression of important pluripotency factors, including Nanog, a master regulator of stem cell identity, and the activation of differentiation-related genes Transcriptional and ... is considered a means of keeping developmental genes poised for rapid acti-vation during stem cell differentiation [20,21], although it is neither a unique feature of ES cells [16,17,23] nor a...

Ngày tải lên: 14/08/2014, 08:21

... positives Identification of X-box regulated genes facilitated the cloning of the C elegans dye filling defective gene, dyf-5 Figure 4 Identification of X-box regulated genes facilitated the cloning of ... of many eukaryotic cells in vertebrates, including kidney and endothelial cells, myocardial cells, odontoblasts, retinal photoreceptor cells and cortical and hypothalamic neurons [1] The biogenesis ... list of candidate cil-iary genes from 293 genes to a final total of 46 genes Although the above studies [16,17] produced large gene sets that contain many known and putative X-box regulated genes,...

Ngày tải lên: 14/08/2014, 17:22

... devel-Cell types of different developmental stages in two models of stem cell differentiation Figure 1 Cell types of different developmental stages in two models of stem cell differentiation (a) Cell ... Pluripotent stem cell C57Bl/6 cell line NSC A Adult neural stem cell *Neurosphere LVB A Adult mature neural cell Lateral ventricles of the brain HSC A Long-term hematopoietic stem cell †Lin- c-Kit+ ... Non-hematopoietic stem cell Bone marrow main population ESC B Pluripotent stem cell CCE cell line FNSC B Fetal neural stem cell *†Hoelow from neurosphere FLHSC B Fetal liver hematopoietic stem cell †Lin-...

Ngày tải lên: 14/08/2014, 20:22

... specialized cells and can divide (through mitosis) to produce more stem cells Trang 5Stem cell potencyTrang 6Stem cell properties Trang 7Different types of stem cellTrang 8Embryonic stem cellsPresenter: ... Trang 1 TYPES OF STEM CELLGROUP 10 Trang 2Introduction Trang 3Introduction of stem cellPresenter: Pham Quynh Hoa Trang 4What are stem cells?• Stem cells are undifferentiated biological cells that ... kinds of stem cellsTrang 10• Embryonic stem cells (ES): the cells of the inner cell mass blastomeres when cultured, which remain pluripotent. • Pluripotent stem cells are generated from germ cells...

Ngày tải lên: 07/11/2014, 14:54

... down-regulated genes are significantly related to cell adhesion, cell motion, cell morphogenesis, regulation of cell death, and stem cell development (Botti et al., 2011) 1.3.2 IRF6 and cell proliferation ... Trang 1Identification of IRF6 Downstream Target Genes in Zebrafish MA YANKUN (Bachelor of Science, Zhejiang University) A THESIS SUBMITTED FOR THE DEGREE OF MASTER OF SCIENCE DEPARTMENG OF PAEDIATRICS ... express in different stages of the cell cycle and consists of checkpoints (Bailey et al., 2008) The entry or exit of the cell cycle plays an important role in regulation of cell proliferation and...

Ngày tải lên: 01/10/2015, 17:26

... Observed number of genes within each bin of 3M E: Expected number of genes within each bin of 3M E= (3M/Length of chromosome)* Number of testes-specific genes on the chromosome Degree of freedom is ... existence of all kinds of regulatory mechanisms of gene expression, the distribution of genes on the chromosome of organisms is not generally random One proof of non-random distribution of genes ... clusters of placenta specific genes Alexander et al found large clusters of testes- specific genes on Drosophila genome by EST profiling [1] Finally Karine et al [21] identified clusters of genes specifically...

Ngày tải lên: 07/10/2015, 10:02

... down-regulated genes are significantly related to cell adhesion, cell motion, cell morphogenesis, regulation of cell death, and stem cell development (Botti et al., 2011) 1.3.2 IRF6 and cell proliferation ... Trang 1Identification of IRF6 Downstream Target Genes in Zebrafish MA YANKUN (Bachelor of Science, Zhejiang University) A THESIS SUBMITTED FOR THE DEGREE OF MASTER OF SCIENCE DEPARTMENG OF PAEDIATRICS ... express in different stages of the cell cycle and consists of checkpoints (Bailey et al., 2008) The entry or exit of the cell cycle plays an important role in regulation of cell proliferation and...

Ngày tải lên: 09/10/2015, 10:49

... in-depth analysis of ethical and safety issues related to clinical application of stem cells Key words: embryonic stem cells, induced pluripotent stem cells, mesenchymal stem cells, stem cell-based ... Knoepfler P Selling Stem Cells in the USA: Assessing the Direct-to-Consumer Industry Cell Stem Cell 2016; 19:154-157 4 Smith AG Embryo-derived stem cells: of mice and men Annu Rev Cell Dev Biol 2001; ... regarding human embryonic stem cell (hESC) research, therapeutic potential and clinical translation of induced pluripotent stem cell (iPSC) and safety issues of mesenchymal stem cell (MSC)-based therapy...

Ngày tải lên: 15/01/2020, 11:56

... synthesis of proline and GABA) Several of these genes were shown to be involved in synthesis of osmoprotectants, which ameliorate the detrimental ef-fects of drought (reviewed by [32]) Up to 29% of ... ad-justed P-value cutoff of 0.05 without setting a cross-species Figure 2 Within species microarray meta-analysis (A) Expression profiles of significantly differentially expressed genes in each species ... percentage (4%) of DEGs, which may be to the outcome of partial representation of transcripts on the Affymetrix array Completion of the wheat genome sequence will facilitate the discovery of additional...

Ngày tải lên: 26/05/2020, 20:57

... gland-rich tissues of A officinalis leaves qRT-PCR analysis showed that 10 of these were specifically enriched in the salt gland-rich tissues Our data suggest that one of the selected genes, namely, ... number of studies have been conducted on the structure of salt glands [3,6,9-12], only a few were regarding their function [13] Therefore, studies such as identification of genes that are specifically ... the identification of two Dehydrins in the genome of A officinalis Characterization of AoDHN1 Tissues collected from two-month-old seedlings that were not exposed to salt were used for tissue-specific...

Ngày tải lên: 27/05/2020, 00:17

... un-controlled proliferation and dedifferentiation of tumor cells by causing changes in the expression of genes that control the G1/S phase of the cell cycle, and epithelial-mesenchymal transition ... each type), degree of fi-brosis and inflammatory cell infiltration (mild, moderate, severe), date of surgery, follow-up duration, date of recur-rence, and date of death The degree of fibrosis was ... edge artifact Each recipi-ent block consisted of 45 cores of tumor tissue (15 cases), and 4 cores of positive controls for stem cell markers, and 1 core of negative control To reduce tissue loss,...

Ngày tải lên: 17/06/2020, 03:03

... 9consensus, identification of CSCs has been difficultdue to lack of specificity of markers and limitations of model systems We reasoned that true HCC CSCs might be enriched in tumor cells that ... characterization of a stem cell population from adult human liver Stem Cells 2006, 24(12):2840 –2850. 25 Endo K, Terada T: Protein expression of CD44 (standard and variant isoforms) in hepatocellular ... promise of liver cancer stem cell markers Stem Cells Dev 2011, 20(12):2023 –2030. 13 Fan ST, Yang ZF, Ho DW, Ng MN, Yu WC, Wong J: Prediction of posthepatectomy recurrence of hepatocellular carcinoma...

Ngày tải lên: 05/11/2020, 08:01