identification of myoblasts using fluorescence in situ hybridization fish

... incu-bated in pretreatment solution at 80 °C for 35 min, rinsed for 3 min in deionised water, incubated 10– 20 min in 0.15% pepsin in 0.1 N HCl solution at 37 °C, and rinsed again for 3 min in ... established in a combinatorial analysis based on percentage of cells containing a genomic abnormality Each cut-off was de-termined by simulating all possible cut-off combinations (for each parameter in ... prioritise in-dividual candidate FISH parameters Inin-dividual FISH pa-rameters were grouped in combinations, and the ROC method was used to (i) select optimal FISH parameter combinations by calculating...

Ngày tải lên: 23/07/2020, 23:51

... prognostic impact of BCR-ABL1 signal patterns detected by fluorescence in situ hybridization (FISH) remains largely unaddressed Methods: BCR-ABL1 signal patterns were analyzed using FISH in 243 CML-chronic ... yx021021@sina.com †Zhanglin Zhang and Zhiwei Chen contributed equally to this work. 2 Institute of Hematology, Academy of Clinical Medicine of Jiangxi Province, Nanchang 330006, China 3 Department of ... significance in the setting of TKIs remains largely unexplored Even under the TKI treatments (imatinib monotherapy or imatinib in combin-ation with low-dose cytarabine or interferon), the fre-quency of...

Ngày tải lên: 17/06/2020, 18:11

... 1M.M Ribeiro, G LeProvost et al.Origin identification of P pinaster stands using cpSSR Original article Origin identification of maritime pine stands in France using chloroplast simple-sequence repeats ... re-sults Use of this test should help when determining the origin of maritime pine stands in the Aquitaine region of France. cpSSR / microsatellites / terpene / origin identification / Pinus pinaster ... programs that have been undertaken in most parts of the range of P pinaster since the beginning of the twentieth century [5] The use of seeds of different origins, together with gene flow, has...

Ngày tải lên: 08/08/2014, 14:20

... Expression of RT-PCR markers by lymph node status Lymph node involvement for all patients in the study had been assessed using haemotoxylin and eosin staining (Table 2) Certain patients had their sentinel ... 5Table 2: Clinical details and RT-PCR positivity data.1 in situ Tis - x x ND - - - -2 in situ Tis - + + ND - - - -3 in situ Tis - - - ND - - - -4 in situ Tis - + + ND - - - -5 in situ Tis - ... of all 5 RT-PCR markers at a level of 10 cells per ml of blood (Figure 1) In samples containing 1 cell per ml of blood (10 cells total), marker expression was detected in 2/3 samples indicating...

Ngày tải lên: 10/08/2014, 22:20

... http://ccforum.com/content/14/1/R10 Page 5 of 11 an internal control of the clustering process and indicates on a gross level that the clustering identifies meaningful groupings of physiology. Clinical evaluation of clusters ... few data points for the proportions to be meaningful. Probability of infection in each cluster Figure 3 Probability of infection in each cluster. The baseline infec- tion rate (dashed line) is 0.735. ... lowest probabilities of death. Significance of correlations was determined using both bootstrapping and label shuffling resampling methods (10,000 iterations of each) to obtain a null distribution...

Ngày tải lên: 13/08/2014, 20:21

... major types included normalizing the trimmed mean and trimmed variance of each chip and using percentage ranks instead of numerical expression levels In the first case, all of the data points were ... averaged 75% of the value of the lower rank (nearer the top of the list) with 25% of the value of the higher rank A final score was obtained by combining the results from each method in this way, ... looking at the variance of a small number of expression levels, especially in studies with very small sam-ple sizes Using these variances as the basis for determining differential expression offers...

Ngày tải lên: 14/08/2014, 14:21

... TFBSs using two-way clustering Starting from the available set of 34 TFBSs identified using sets of co-expressed genes (see text for details), clusters of genes with similar TFBS combinations in ... shows the identification of the TFBS HA_HSE2 involved in zygotic embryogenesis The top picture depicts a subset of all 573 Arabidopsis genes containing the module consisting of two distinct G-boxes ... which is an element inducing gene expression during zygotic embryogenesis [34] Initially, 573 Arabidopsis genes were grouped containing a combination of two distinct G-boxes in their promoters...

Ngày tải lên: 14/08/2014, 17:22

... however, are limited in their ability to probe the contributions and dynamics of individual molecules Examination of intact individual molecules in DOM has proved challenging, leading researchers ... contributions of N- and S-containing elemental formulae than the riverine / estuarine DOM samples We were intrigued to find a substantial contribution of sulfur relative to nitrogen in the deep marine ... values in surface seawater and show that many of these are present in pure cultures of the marine -proteobacterium Candidatus Pelagibacter ubique when grown in natural seawater These findings indicate...

Ngày tải lên: 20/10/2022, 04:29

... load, is measured in using other techniques A system for continuously monitoring the ⁎ Corresponding author at: Institut of Chemical Engineering and Technology, University of the Punjab, Quaid-i-Azam ... techniques are heat setting, impregnating, ri-gidity in the felt Impregnating involves chemical treatment for from the dust side of the needle felt Calendering is the pressing of the felt for improved ... pressure of the cleaning air can be set up to 4 bar, time of cleaning pulse can be set between 10 and 100 ms, and interval between cleaning pulses between 2 and 450 s view® software according to DIN...

Ngày tải lên: 02/11/2022, 10:41

... Airport site in Houston during the Texas Air Quality Study (TexAQS) 2000 The analysis determined the profiles and contributions of nine sources and the corresponding wind speed, wind direction, ... concentration of compound j measured in sample i, the concentration of compound j in the emission of source p, and the strength of source p on sample i (16, 19). In this section, wind direction and wind ... wind speed of sample i The ranges of wind direction and wind speed are divided into a series of subranges having similar numbers of samples in each Then each wind direction/ speed belongs to a...

Ngày tải lên: 22/03/2014, 14:20

... bằng Biotin thì người ta sử dụng Avidin,Streptavidin, Extra-Avidin và kháng thể của Biotin để phát hiên [1], [29].Nếu mẫu dò được đánh dấu bằng Digoxigenin thì sử dụng kháng thể củaDigoxigenin để ... để đánh dấu như Biotin, Digoxigenin…  Digoxigenin (DIG) Digoxigenin liên kết Uridine ở vị trí C-5 qua một cánh tay bao gồm 11nguyên tử cacbon [25] Trang 33Hình 2.4 Digoxigenin – UTP/dUTP/ddUTP, ... dụng thường là chloroform Ngấm paraffin: Mục đích cho dung môi của paraffin trong mô được tẩy hết  Đúc mẫu Thực chất là vùi miếng mô vào một khối paraffin để sau khi vùi paraffin vàmiếng mô liên...

Ngày tải lên: 28/06/2014, 15:23

... trình lai được tiến hành rất tốt và không bị ngoại nhiễm Trên cơ sở thử nghiệm lại quy trình In situ hybridization, chúng tôi nhận thấy rằng khả năng chẩn đoán bệnh đốm trắng trên tôm sú của phương ... tự không phải DNA đích trong mẫu mô, tạo ra các tín hiệu nền hoặc do thời gian cắt mẫu với Proteinase K quá lâu, cũng có thể do thao tác nhuộm màu bổ sung chưa tốt Trong quá trình thực hiện, ... tốt làm hư cấu tạo của mô Điều này cũng có thể do trong quá trình lai, chúng ta cắt mẫu bằng Proteinase K quá lâu hoặc thao tác cắt mẫu chưa được thực hiện tốt nên cấu trúc mẫu mô không còn rõ như...

Ngày tải lên: 28/07/2014, 02:21

... sử dụng avidin (hay streptavidin) – biotin: đây là một cặp ligand có hằng số ái lực cao nhất tìm thấy trong sinh học Đầu tiên, mẫu dò được đánh dấu bằng biotin theo hai cách: biotin được gắn ... Paraffin được dùng trong quy trình xử lý mẫu này là hỗn hợp với sáp ong theo tỷ lệ 5 paraffin : 1 sáp ong III.5.2.3 Đúc mẫu trong paraffin Đặt mẫu đã xử lý vào khuôn inox, dùng bình rót paraffin ... đậm đặc 10 lần (10X PBS Buffer) Proteinase K (dạng bột) Đệm PBS 1 lần (1X PBS Buffer) Đệm PBS/Glycine đậm đặc 10 lần (10X PBS/Glycine Buffer) Dung dịch lai (Hybridization Solution) Mẫu đối chứng...

Ngày tải lên: 28/07/2014, 02:21

... ml-1 in Assay medium 1 Cells were permeabilized by incubation in 20 μg ml-1 of alamethicin (Sigma) for 10 min at room tem-perature before staining Staining with Yo-Pro-1 (Molecu-lar Probes Inc, ... synthase in situ Digi-tonin was chosen for comparison when following UDP-glucose incorporation, since this agent has been used in many investigations The activity measured (incorpora-tion of labeled ... Omitting oryzalin during permeabilization and assay gave similar inhibition (81 ± 7% of DMSO control) show-ing that oryzalin did not interfere with the assay In con-Callose synthesis measured in...

Ngày tải lên: 12/08/2014, 03:20

... stress per se In another study the expression of 150 genes in response to wounding and insect feeding was carried out [58], finding that some of the responding genes, for which the inducing stimulus ... BU671860] and a zinc finger protein specifi-cally induced under chilling treatment The large amount of TFs identified in Arabidopsis, indicating the complexity in the secondary metabolism of the plants ... showing 287 genes whose expression lev-els were studied in three treatments: control (Ctrl), cold (C) and salinity (S) The ordination was obtained taking into account the three (two in case of...

Ngày tải lên: 12/08/2014, 05:20

... allergic skin-prick test to that induced by histamine N: number of subjects in each response group according to the skin-prick test using potato extract Positive cutoff values were determined from ... SIF treatments in the presence or absence of heating A combination of SGF and heat treatment resulted in the disappearance of the protein bands and the IgE-binding components in extracts from ... followed as in the ELISA As a control, samples were preincubated with equal volumes of PBS (pH 7.5) instead of house dust mite or potato extracts The percentage of inhibition of serum IgE binding was...

Ngày tải lên: 13/08/2014, 13:22

... proportion of dividing cells in thick-the basal layer [32] We suggest that H19 could participate in the regulation of IGF2 transcription by maintaining the genomic imprinting of its promoter in adult ... syndrome, infantile, with sensorineural deafness Kidney and inner ear 5 GRIN2 16 G-protein-regulated inducer of neurite outgrowth Brain 1 PPEF2 31 Protein phosphatase, EF-hand calcium binding domain ... 2 Retina 3 CDC42BPG 41 CDC42 binding protein kinase gamma Heart and skeletal muscle 3 TMPRSS5 53 Transmembrane protease, serine 5 (spinesin) Spinal chord 2 TEC 59 Tec protein tyrosine kinase...

Ngày tải lên: 14/08/2014, 07:21

... parameters were estimated by minimising minus log-likelihood L in [9] and L2 in !12!, using a quasi-Newton minimisation routine A reparameter-isation was made using the difference between homozygotes ... therefore be distinguished This was accomplished by simulating different residual variances in F and F Four situations were investigated, combining all combinations of non-normality in F and increased ... with t = 25 and t = 35 Each point in the figure is on average of 100 replicates The power increased with increasing number of observations Increasing the number of observations > 1000 gave...

Ngày tải lên: 14/08/2014, 20:20

... region of two types of introns: ones that contain a PY tract with affinities similar to those derived using SELEX, and those with PY tracts with lower affinities Binding of U2AF65 to low-scoring ... located in the region upstream of the branchpoint itself Since most branchpoints are located Binding of U2AF65 to human PY tracts validates the U2AF65 SELEX scoring system Figure 2 Binding of U2AF65 ... of human intron PY tracts using a U2AF65 binding site scoring method U2AF65 plays an important role during splicing and is known to bind to the PY tract region located between the branch-point...

Ngày tải lên: 14/08/2014, 20:22

... 90% identity in the Runt domain Moreover, all RUNX proteins have PPxY motif, a domain for the binding of WW domain-containing proteins, such as Yes-associated protein (YAP), within their transcription ... transcription activation domain Figure 1.5: RUNX1 domains and interactions A diagram of RUNX1 protein with main functional domains, interacting proteins and sites of phosphorylation and acetylation ... core-binding protein-α; FAB, French–American–British; FLT3, FMS-related tyrosine kinase 3; GATA1, GATA-binding protein 1; HOX, homeobox; ITD, internal tandem duplication; MLL, mixed lineage...

Ngày tải lên: 12/09/2015, 08:19