identification—mass spectrometry for proteomics analysis

... in trypsin solution for digestion of proteins to peptides for subsequent processing and mass spectrometry analysis See Experimental Methods for details Kooren et al Clinical Proteomics 2011, 8:13 ... Page of 11 Kooren et al Clinical Proteomics 2011, 8:13 http://www.clinicalproteomicsjournal.com/content/8/1/13 Shotgun proteomics analysis: Tandem mass spectrometric analysis and sequence database ... J.A.K We also thank the Center for Mass Spectrometry and Proteomics at the University of Minnesota for instrumental resources and the Minnesota Supercomputing Institute for computational support Author...

Ngày tải lên: 13/08/2014, 13:20

... SFE conditions, except for propham From the comparison of both methods, it was found that although higher recoveries were obtained by MAE, recoveries by SFE were good (for propoxur, methiocarb ... methiocarb and chlorpropham) under their respective optimum conditions Therefore, both MAE and SFE are suitable techniques for extracting carbamate pesticides from soil However, when extraction is ... employed to optimize both microwave-assisted extraction and supercritical fluid extraction conditions for extracting carbamates (propoxur, propham, methiocarb, and chlorpropham) from spiked soil An...

Ngày tải lên: 16/09/2015, 17:13

... chromatogram of the above molecular ions at m/z 60, 151, and 210 For the LC separation of propoxur and its two transformation products (N-methyl formamide and 2-isopropoxyphenol), a mixture of ultrapure ... probably resulted from the neutral loss of 1-propene, similar to the formation of a fragment ion of propoxur at m/z 168 For N-methylformamide, one peak at m/z 60 [M+H]+ was detected in the MS spectrum, ... tested compound at m/z 210, m/z 151 and m/z 60, were selected for quantitative analysis Fig 5-2 shows the individual SIM chromatogram for all three compounds in a mixture of 0.10 ng/µl of a solvent-based...

Ngày tải lên: 16/09/2015, 17:13

... Extraction was performed at 115°C with a heating time of for PAHs and PCBs, or for triazines at 80% power After the extraction, the vessels were cooled down to room temperature before they were ... inside) and MAE was performed at 95 °C for at 80% power After the extraction, the vessel was allowed to cool down to room temperature before it was opened 4.1.2.3 HPLC measurements For triazines: A ... Methiocarb; 4) Chlorpropham (c) After SFE 30 107 4.2.3.4 Data analysis for SFE optimization The conditions for the SFE technique should be optimized before carrying out any comparative study with other...

Ngày tải lên: 16/09/2015, 17:13

... LC-APCI-MS for the determination of carbamates: carbofuran and 3hydroxy-carbofuran, and the analysis of triazine herbicides in water [8] LC-ESI-MS has been also successfully applied for the simultaneous ... propoxur using LC-APCI-ITMS and LCESI-ITMS (a) transformation products by positive ESI (b) transformation products by positive APCI (c) transformation products by negative APCI 58 concentration ... To identify the major TPs of propoxur by mean of LC-API-ITMS The performance of two MS ionization techniques (ESI and APCI) for such identification purposes are compared; (2) To investigate the...

Ngày tải lên: 16/09/2015, 17:13

... finally stored in polyethylene bottles after being weighed accurately No sieving was performed before analysis To assess possible contamination from sample preparation, together with each series ... (Millipore, Bedford, MA, USA) water purification system All the samples (solutions and extracts) were filtered through 0.45-m membranes (Millipore) and degassed in an ultrasonic bath before use 2.3 ... samples prepared above and ultrapure water All the samples were degassed by an ultrasonic bath before LC-MS analysis 2.3.2 Preparation of Soil Samples 2.3.2.1 Blank soils Blank soils, collected from...

Ngày tải lên: 16/09/2015, 17:13

... searching for a suitable technique for the analysis of mixtures that, often contain unknowns and/or analytes in low concentration, the combination of chromatography (for separation) with mass spectrometry ... over gas chromatography (GC) in analysis of samples with high polarity and low volatility In contrast, one of the prerequisites for mass spectrometric analysis is the formation of volatilized ions ... method for liquid introduction into an MS Additionally, ESI is especially suited for the analysis of compounds ionized in the liquid phase, due to the fact that, the observed ions, generally formed...

Ngày tải lên: 16/09/2015, 17:13

... for protein and other biomolecular analysis The true power of mass spectrometry for protein characterization is the utilization of tandem mass spectrometry (MS/MS) and proteolytic digestion for ... Cells by HDAC Inhibitors 117 4.2.10 Sample Preparation for Mass Spectrometry .117 4.2.11 Mass Spectrometry Analysis .118 4.2.12 Data Analysis 119 4.3 Results 119 4.3.1 ... .45 3.2.12 Cell Cycle Analysis .45 3.2.13 Propidium Iodide Staining of Cells for FACS Analysis .45 3.2.14 MTS Assay 46 iii 3.2.15 Sample Preparation for Mass Spectrometry 46 3.2.15.1...

Ngày tải lên: 04/10/2015, 15:46

... samples for analysis, and helped analyze the mass spectrometry data TSC prepared samples for analysis, analyzed the mass spectrometry data and helped to draft the manuscript SV assisted with the analysis ... digestion of gel slices and tandem mass analysis (LC-ESI-QIT MS) for protein identification had been used successfully before on other VV proteins [11] A second MS analysis was done in parallel with ... against 800 mL of 20 mM Tris (pH=8.0) for hours at room temperature, and changed three times The final dialysis was performed for 16 hours at 4°C Dialysis was performed to remove as much of the OG...

Ngày tải lên: 19/06/2014, 08:20

... Agilent shall not be liable for errors contained herein or for incidental or consequential damages in connection with the furnishing, performance or use of this material Information, descriptions ... temperature of -5oC, and a narrow ID torch to maintain plasma stability Performance The ICP-MS sample introduction setup for the analysis of volatile organic solvents such as isopropyl alcohol (IPA) ... addition, which is optimized for each different sample type With this setup, the analysis of organics is stable and reproducible Figure shows a 4-hour stability plot for a series of trace elements...

Ngày tải lên: 05/03/2014, 21:20

... according to the formula: yB + * sB and were 0.2 μg/L for 2,4-TDA, 0.2 μg/L for 2,6-TDA, 0.3 μg/L for 4,4’-MDA, 1.0 μg/L for 1,5-NDA, 1.0 μg/L 1,6 HDA and 1.0 μg/L for both IPDA isoforms The levels ... was 123 (m/z for target ion) and 286 (m/z for qualifier), respectively; for 4,4’ MDA the RT was 18.47 and m/z was 490/ 252; for 2,4- und 2,6-TDA: 10.33 and 10.77 (with m/z 295/414); for 1,6-HDA, ... between 0.5 and 30 ppb for to 120 minutes The averaged cumulative median concentration was 5.5 ± 5.1 ppb (see additional file 1, 2, and for details on the method) For data analysis, the study subjects...

Ngày tải lên: 20/06/2014, 00:20

... identification by SOM and MPRQ For “No of Complete Correct” and “Complete Correct Accuracy”, first-rank peptide was used for analysis For specificity and sensitivity, the results for “first-rank peptide ... on my work in the analysis of biological sequences, with special concentration on algorithms for peptide and PTM identification using tandem mass spectrometry The main concern for algorithms in ... and analysis of biological data In bioinformatics, bioinformaticians are provided with a huge amount of raw data that are generated by various experiments on different biological samples Bioinformaticians...

Ngày tải lên: 12/09/2015, 08:18

... MALDI-TOF MS, was 11 947.87±2 for (Met1)-ONC (M23L) and 11 838.21±2 for rONC, which conforms well to the expected values: 11 949.90 for (Met1)-ONC (M23L) and 11 836.82 for rONC with a glutaminyl ... pyroglutamyl formation to the conformational stability of active onconase In order to evaluate the contribution of the (Met1) hydrolysis and pyroglutamyl formation to the global conformational ... were performed on a LF-3000 Beckman-Coulter sequencer, connected online to an HPLC apparatus for the identification of ` the phenylthiohydantoins, at the Servei de Proteomica ` ` i Bioinformatica...

Ngày tải lên: 19/02/2014, 12:20

... (5S)-[5-2H1]F420H2 is for a : mixture of [5-1H]F420 and [5-2H]F420 The other two (a, c) are for [5-1H]F420 (C) Experiment with NaB2H4-reduced [5-1H]F420 showing that the mass spectrum of F420 formed from ... oxidation product (c, white) are for a : mixture of [5-1H]F420 and [5-2H]F420 (Fig 2Bb, stick spectrum, black) For comparison the relative intensities calculated for a : mixture are given (Fig ... Chemischen Industrie Henning Seedorf thanks the Deutsche Forschungsgemeinschaft for a graduate fellowship We are indebted to Christoph Hagemeier for providing purified F420-dependent Mtd from Methanopyrus...

Ngày tải lên: 20/02/2014, 03:20

... 13.1 ± 0.8% for KidT46G ⁄ T69G and 13.9 ± 0.8% for KidA55G ⁄ T69G, similar to values obtained with the single mutants (13.4 ± 1.2% for T46G, 12.3 ± 0.8% for T69G and 11.9 ± 1.5% for A55G) (Fig ... interactions required for catalysis and therefore have a drastic effect on the RNase activity of the toxin and a moderate or null effect on RNA binding Kid mutants suitable for the analysis should ... separation techniques) The MS analysis is efficient and very sensitive, and it was particularly useful for comparisons of the different mutational variants of the same protein For RNA binding assays,...

Ngày tải lên: 16/03/2014, 03:20

... tissues as well as their analysis by mass spectrometry, for it is this combination of biochemistry and mass spectrometry that is driving progress in the field of functional proteomics today Special ... information has made mass spectrometry and biology much more compatible than in the past The Ôjoined forcesÕ of sophisticated biochemical and mass spectrometry approaches are the real driving forces ... FEBS 2003 Affinity purification-mass spectrometry (Eur J Biochem 270) 575 Table Merits of mass spectrometry based protein identification strategies used for the analysis of protein complexes MALDI-TOF...

Ngày tải lên: 31/03/2014, 07:20

... resulted m development of the permethylatlon procedure for peptlde analysis by MS (5) For me, personally, the fortuitme paper has also been very fortmtous Shortly after Its publication, I became involved ... new posslblhtles for mass spectrometric analysis had to come from surface science Anyway, among other subjects, he started to investigate surface analysis by secondary-ion mass spectrometry (SIMS) ... spectrometric techniques used for analysis of peptides and protems Another technique that allowed the analysis of large, mvolatile organic molecules was plasma-desorption mass spectrometry (PDMS) developed...

Ngày tải lên: 11/04/2014, 10:11

... pattern analysis by MALDI-ToF spectrometry for the differentiation between early breast cancer patients and healthy controls Most importantly, a classifier built on this analysis outperforms those ... mass spectrometry profiling for cancer detection Expert Rev Proteomics 2006, 3:311-320 Dworzanski JP, Snyder AP: Classification and identification of bacteria using mass spectrometry- based proteomics ... Dickinson), incubated for 30 at room temperature to allow clotting, and then centrifuged at 1000 g for 10 to remove the clot The serum was aliquoted and stored at -70°C Directly before analysis, samples...

Ngày tải lên: 18/06/2014, 15:20

... spectrometry in clinical proteomics from the present to the future Proteomics Clin Appl 2009, 3:6-17 Li J, Zhang Z, Rosenzweig J, Wang YY, Chan DW: Proteomics and bioinformatics approaches for identification ... used for sample identification and classification [10-12] Mass spectrometric methods particularly suitable for proteome pattern analysis are Matrix-Assisted Laser Desorption Ionization spectrometry ... and identification of bacteria using mass spectrometry- based proteomics Expert Rev Proteomics 2005, 2:863-878 Somorjai RL: Pattern recognition approaches for classifying proteomic mass spectra of...

Ngày tải lên: 18/06/2014, 16:20