human diseases and protein functions

... α2-macroglobulin and α1-antitrypsin were detected in bronchial washings after surgery After quantification, the total amounts of α2-macroglobulin at bands 2, 4, and 5 and antitrypsin at bands 5, 7, and 8 ... Ratio of expression of α1-antitrypsin at band 5 to that at bands 7 and 8 Table 3 Ratio of the expression of α1-antitrypsin at band 5 to that at bands 7 and 8 in the lobectomized patients without ... Ratio of expression of α1-antitrypsin at band 5 to that at bands 7 and 8 Table 4 Ratio of the expression of α1-antitrypsin at band 5 to that at bands 7 and 8 in lobectomized patients with acute

Ngày tải lên: 22/06/2014, 04:20

... Trang 1human lung fibroblasts is specifically mediated by theand protein kinase A Elena Sokolova1, Roland Hartig2and Georg Reiser1 1 Institut fu¨r Neurobiochemie, ... ATCC and 1321N1 cells were cultured in DMEM supplemented with 10% fetal bovine serum and 100 lgÆmL)1 penicillin and streptomycin and kept at 37C in a humidified atmo-sphere of 5% (A549 cells) and ... that prostaglandin (PG)E2, via the prostanoid receptor EP2 and subsequent cAMP elevation, downregulates mRNA and protein levels of PAR-1 in human lung fibroblasts Under these conditions, the functional

Ngày tải lên: 30/03/2014, 04:20

... protein [14] Given the presence of at least seven protein–protein interaction domains including the KIND, FERM and five PDZ domains, PTP-Bas/BL functions most probably as a major scaffolding protein ... interacting protein-6; ZRP-1, zyxin related protein-1. Trang 4A number of interacting proteins were shown to interact with the PDZ2 domain of PTP-Bas/BL The human cell surface protein Fas was the first protein ... higher eukaryotes Protein target phosphorylation is cata-lysed by protein tyrosine kinases and counteracted by the activity of protein tyrosine phosphatases [1] The family of protein tyrosine

Ngày tải lên: 30/03/2014, 20:20

... Intracellular transport and TGN localization of UL20p mutants and gK Transport and localization of UL20p and gK was further assessed by transient coexpression of gK and UL20p and simultaneous detection ... [13,14], and the UL53 gene coding for glycoprotein K (gK) [15-19] Of these four membrane associated proteins, only UL20 and gK are absolutely essential for the intracellular envelopment and transport ... AccessResearch The herpes simplex virus UL20 protein functions in glycoprotein K (gK) intracellular transport and virus-induced cell fusion are independent of UL20 functions in cytoplasmic virion envelopment

Ngày tải lên: 18/06/2014, 18:20

... Trang 1WHEN THINGS GO WRONG – DISEASES AND DISORDERS OF THE HUMAN BRAIN Edited by Theo Mantamadiotis Trang 2When Things Go Wrong – Diseases and Disorders of the Human Brain Edited by Theo Mantamadiotis ... anxiety and tears, is none other than the brain It is specially the organ which enables us to think, see and hear, and to distinguish the ugly and the beautiful, the bad and the good, pleasant and ... processes and emotions The last and the most important is that the executive functions of the PFC develop to their full capabilities throughout the juvenile and adolescent period in humans This

Ngày tải lên: 28/06/2014, 11:20

... lines and culture Human HeLa (S3), VA13, XP-A, and HEK293 cells (obtained from the American Type Tissue Collection), and cisplatin-resistant HeLa cell lines (HR3 and HR18) [21,22] were prepared and ... NKS, CLS, and CCKC conceived and designed the experiments NKS, CLS, and CHL performed the experiments NKS, CLS, LMP, and CCKC analyzed the data CCKC wrote the paper All authors have read and approved ... Admon A, Linn S: Chromosomal localization and cDNA cloning of the genes (DDB1 and DDB2) for the p127 and p48 subunits of a human damage-specific DNA binding protein Genomics 1995, 29:62-69 Hwang

Ngày tải lên: 10/08/2014, 05:21

... function and T cell parameters in a natural human experimental model of seasonal infectious diseases and nutritional burden Pa T Ngom1*, Juan Solon1, Sophie E Moore1, Gareth Morgan1, Andrew M ... alternating seasons of relative food availability and low infectious diseases burden (har-vest/low infection; January to June) and deprivation and high infectious diseases (hungry/high infection season; ... Prentice1,2 and Richard Aspinall3 Abstract Background: The study exploits a natural human experimental model of subsistence farmers experiencing chronic and seasonally modified food shortages and infectious

Ngày tải lên: 10/08/2014, 05:21

... signalling, osmotic and oxidative stress signalling and pathogen defence signalling pathways In contrast, NADH, Reisky iron sulphur protein and quinone protein are related to mitochondrial and chloroplast ... the experimental work and drafted the manuscript AB and IA helped in the RT-PCR and DD-PCR analysis SM and RWB together designed the experiments IA and SM had proof-read and finalized the manuscript ... second column lists the number and percentage of input genes or proteins involved in the pathway (top red in color) and the number and percentage of background genes or proteins involved in the pathway

Ngày tải lên: 11/08/2014, 21:21

... of putative structure and mutation map of human metapneumovirus F proteinFigure 1 Schematic representation of putative structure and mutation map of human metapneumovirus F protein SS = signal ... identity between and within all groups, with overall min-imum identity of 93.7% and mean identity 96.3% Amino acid identity within major groups was 98.7% for A1 and A2, and 99.3% for B1 and B2 The ... 93.9%) between A1 and A2, and mean 97% (minimum 93.5%) between B1 and B2 The B2 lineage diverged more from the A lineages than the B1 lineage B2 mean identity with A1 and A2 was 86.7% and 89.7%, respectively,

Ngày tải lên: 12/08/2014, 04:20

... is nearly free of bacterial proteins (Fig- ure 3A, lane 7). Trace levels of additional protein bands above and below the main Tat band are multimers and degraded protein products of Tat, respectively, ... tiple and often overlapping and complementary biologi- cal functions [30]. The presence of charged and hydrophobic amino acid residues in Tat makes protein purification difficult as this protein ... residues [9]. To understand if amino acid variation between B- and C-Tat proteins could influ- ence the secondary structure of the proteins and conse- quently their biological functions, we measured

Ngày tải lên: 13/08/2014, 09:20

... donors CO and MCJ provided confocal and fluorescence microscopy analyses PFW and APFT partici-pated in the experiments of RT-PCR for mRNA Hsp65 detection AAMC and CLS provided critical input and assistance ... cross-priming in which plasmid DNA transfects a somatic cell and/or a professional APC and the secreted protein is taken up by other professional APC and pre-sented to T cells [13-16] Early studies conducted ... up by CD11b+(macrophages) and CD11c+ (DC) cells, as well as by B lymphocytes after its administration in mice [23] However, several studies in nonhuman pri-mates and human clinical trials have

Ngày tải lên: 14/08/2014, 19:22

... 200, 100, and 40 Samples were prepared in a 96 well plate and readings were taken at 595 nm The standard curve was calculated and the final protein concentrations were determined SDS-PAGE and Western ... serine/threonine protein kinase, then undergoes autophosphorylation and phosphorylates other downstream NHEJ proteins DNA-PK, specifically, has been implicated in the phosphorylation and activation of proteins ... formed in the presence and absence of 22 pmol 30-bp double strand DNA 30A and 30C (Table 1) and 3 g BSA as indicated Reactions were incubated for 30 minutes at room temperature and stopped by the

Ngày tải lên: 24/08/2014, 11:02

... STUDY OF GENE AND PROTEIN VACCINES FOR ALLERGIC DISEASES IN MICE TAN LI KIANG NATIONAL UNIVERSITY OF SINGAPORE 2007 Trang 2THE STUDY OF GENE AND PROTEIN VACCINES FOR ALLERGIC DISEASES IN MICE ... 2000) and 3 Cytokines and transcription factors that exert potent influences on the efficiency of Th1 and Th2 development (Le Gros G et al., 1990; Paul WE and Seder RA, 1994; Glimcher LH and Singh ... receptor-like protein DEC-205 (Jiang W et al., 1995) as well as adhesion (CD11a and CD54) and accessory molecules (CD40, CD80 and CD86) (Steinman RM, 1997) DCs in culture exist in two functional and phenotypically

Ngày tải lên: 14/09/2015, 14:20

... 39 proteins (27 identified) Proteomics – Human Diseases and Protein Functions Human tissues (Methods) 10 carotid plaques vs reference synthetic gel Results Known functions Identification of proteins ... from orders@intechweb.org Proteomics – Human Diseases and Protein Functions, Edited by Tsz-Kwong Man and Ricardo J Flores; Subject Editors: Tsz-Kwong Man and Ricardo J Flores p cm ISBN 978-953-307-832-8 ... and proteins, and the importance of posttranscriptional regulations and protein modifications in protein functions and human diseases, proteomics has become increasingly important in the research...

Ngày tải lên: 22/06/2014, 04:20

... reliably and economically than conventional neurological assessments, thereby significantly reducing the risks and costs of human clinical trials 92 Proteomics – Human Diseases and Protein Functions ... Proteomics – Human Diseases and Protein Functions to identify proteins that were differentially expressed in the tissues after treatment with various small molecules and siRNAs Proteomics research and ... 90 Proteomics – Human Diseases and Protein Functions The importance of mild and moderate TBI Research in the field of TBI has long been dominated...

Ngày tải lên: 22/06/2014, 04:20

... – Human Diseases and Protein Functions Fig 10 Semiquantitative RT-PCR (A) and Western Blot (B) analyses of SHEE and SHEEC cells The results confirmed the proteomic data that BRE, prohibitin and ... response and this may explain why the transgenic mice was more resistant to liver toxic drugs 126 Proteomics – Human Diseases and Protein Functions Fig 12 In situ hybridization (A–D and G–I) ... samples and 2-DE was correspondingly performed three times (Tang et al., 2009) 128 Proteomics – Human Diseases and Protein Functions Fig 14 Semi-quantitative RT-PCR revealed that the proteins...

Ngày tải lên: 22/06/2014, 04:20

... beat Favoprotein subunit complex II Acyl-CoA-binding protein PHS CBR1 HUMAN KCRB HUMAN GSTP1 HUMAN GDIB HUMAN DHSA HUMAN RL27A HUMAN 60S ribosomal protein L27a RSSA HUMAN IF4G1 _HUMAN NPM HUMAN 40S ... Proteomics – Human Diseases and Protein Functions Protein name ACADV HUMAN VLCAD ATPA HUMAN ATP5A1 THIL HUMAN Acetoacetyl-CoA thiolase Macrophage migration MIF HUMAN inhibitory factor ATPB HUMAN ATPB-3 ... immortalized and cancer cell model, we 267 Proteomic Study of Esophageal Squamous Cell Carcinoma Accession no Protein name MW/PI Scores TPM3 HUMAN TPM4 HUMAN K2C8 HUMAN FSCN1 HUMAN LEG1 HUMAN CLIC1 HUMAN...

Ngày tải lên: 22/06/2014, 04:20

... 290 Proteomics – Human Diseases and Protein Functions and biochemical analyses of human B cell-derived exosomes Potential implications for their function and multivesicular body formation ... primary transcript contains 16 exons and in the adult human brain, 304 Proteomics – Human Diseases and Protein Functions alternative splicing of exons 2, and 10 gives rise to six tau isoforms ... differences rely on the fact that proteins are labeled with fluorescent dyes and all the samples are separated at 300 Proteomics – Human Diseases and Protein Functions the same time in the same...

Ngày tải lên: 22/06/2014, 04:20

... separated and then identified by liquid chromatography – tandem MS (LC-MS/MS) Although the proteolytic 394 Proteomics – Human Diseases and Protein Functions In suspension cells Preparation of proteins ... chance (and vice versa) Ultimately, the scores for each peptide matching the same protein are summed 404 Proteomics – Human Diseases and Protein Functions An illustration of the rigorousness of protein ... datasets and suggest that the trypsin susceptibility of mammalian transmembrane proteins does not significantly differ from that of non-transmembrane proteins 402 Proteomics – Human Diseases and Protein...

Ngày tải lên: 22/06/2014, 04:20