human dendritic cell culture and bacterial infection

... the limits of the culture period C Cell Banking System Procedures: Generation and Characterization of Master Cell Banks (MCB), Working Cell Banks (WCB), and Producer Cells Cell banking systems ... Monitoring of cell identity and heterogeneity: Both manufacturing and testing procedures should be implemented which ensure the control of cell cultures with regard to identity and heterogeneity Cell ... manipulation and subsequent reintroduction of somatic cells into humans have been used or proposed Somatic cell therapy is the administration to humans of autologous, allogeneic, or xenogeneic living cells

Ngày tải lên: 24/10/2019, 10:26

... features and includes stem cells (I-cells) and neuronal cells (N-cells) The non-neuronal phenotype (S-cell) comprises cells that are non-tumorigenic with features of glial/smooth muscle precursor cells ... intrinsic differentiation and tumorigenic properties with use of large panel of NB cell lines Methods Cell culture and differentiation The thirteen different human NB cell lines or clones, established ... not present in S-cells N- and I-cells are tumorigenic and are neuroblas-tic in terms of their morphological and biochemical cha-racteristics, whereas S-cells are non-tumorigenic and lack neuroblastic

Ngày tải lên: 05/11/2020, 00:17

... melanoma cell lines depending on their origin [18] Methods Cell lines and cell culture conditions The selected cell lines included cells of primary and meta-static origin Thirteen human melanoma cell ... examples are shown in Figure 2 (C and D) and Figure 3 (C and D) Surface and intracellular expression of chemokines and their receptors in WM-115 and WM-266.4 cell lines after xenotransplantation ... their cell surface However, melanoma cell lines show intracellular expression of all the aforementioned receptors and most of their respective ligands When analyzing the xenografts and the cell

Ngày tải lên: 05/11/2020, 01:20

... (B), DCs (C), T cells (D), eosinophils (E), and macrophages (F) from the bone marrow (BM); and B cells (G), CD4+T cells (H), CD8+T cells (I), neutrophils (J), monocytes (K), and NK cells (L) from ... endothelial and stromal cells [17–19] In the steady Trang 2state, JAM-C mainly interacts with JAM-B [20] at cell-cellcontacts Moreover, JAM-C and JAM-B can also bind the integrins aMb2(Mac-1) and a4b1(VLA-4), ... Trang 1The recruitment of dendritic cells to sites of infections and their migration to lymph nodes is fundamental for antigen processing and presentation to T cells In the present study, we

Ngày tải lên: 02/11/2022, 08:51

... virus (HCV) and human T-cell lymphotropic virus type 1 (HTLV-1) coinfection: Impact on liver disease, virological markers and neurological outcome Authors: Ot´avio M Esp´ındola, Alexandre G Vizzoni, ... C virus (HCV) and human T-cell lymphotropic virus type 1 (HTLV-1) coinfection: Impact on liver disease, virological markers and neurological outcome Otávio M Espíndola a,1 , Alexandre G Vizzoni ... Middle East and South America, particularly in Brazil.1 Trang 4HTLV-1 causes a persistent infection of CD4+ and CD8+ T-cells that leads to diverse changes in the immune response,2 and can be associated

Ngày tải lên: 04/12/2022, 10:37

... vitro generation of cytotoxic and regulatory T cells by fusions of human dendritic cells and hepatocellular carcinoma cells Address: 1 Division of Gastroenterology and Hepatology, Department of ... lymphocytes (CTL) and regulatory T cells (Treg) by fusions of dendritic cells (DCs) and HCC cells was examined Methods: HCC cells were fused to DCs either from healthy donors or the HCC patient and investigated ... the HCC cell culture (HCCsp) influenced on the function of DCs/HCC fusion cells (FCs) and generation of CTL and Treg Results: FCs coexpressed the HCC cells-derived WT1 and CEA antigens and DCs-derived

Ngày tải lên: 18/06/2014, 15:20

... impact of beads and anti-CD3 on cells, we serially monitored for-ward scatter (a relative measure of cell size) in CD4 and CD8 cells after stimulation and restimulation CD4 and CD8 T cell size increased ... exten-sive anti-CD3-T cell receptor crosslinking on a surface, and the costimulation provided by cell-cell interaction between T cells and Fc receptor positive accessory cells such as monocytes ... Monitoring T cell division and early expansion using CFSE labeled cells To monitor cells division and expansion during the early days after stimulation, cells were CFSE-labeled and moni-tored

Ngày tải lên: 18/06/2014, 16:20

... HHV-6A- and mock-infected cells (Fig 3, lane 1 and 2) All detected proteins, e.g 44 and 88 kD, were regarded to be cellular proteins Infections of JJHAN cells with HHV-6A (U1102) Figure 1 Infections ... mock-infected JJHAN cells at 3 dpi Note the CPE, i.e enlargement of cells, in HHV-6A infected cells D and E Detection of HHV-6 antigen gp60/110 in HHV-6A infected cells HHV-6A-and mock-infected cells were ... after 3 hpi and 1, 3, 5 and 7 dpi are shown Results are based on three experiments and presented as mean ± standard deviation (error bars) B and C Light microscopic analyses of HHV-6A- and mock-infected

Ngày tải lên: 18/06/2014, 18:20

... FM: Global epidemic of human T-cell lymphotropic virus type-I (HTLV-I) J Emerg Med 2000, 18(1):109–119 11 Matsuoka M: Human T-cell leukemia virus type I and adult T-cell leukemia Oncogene ... HTLV-1 in viral replication, T cell activation, and cellular gene expression Front Biosci 2004, 9:2556–2576 22 Mortreux F, Gabet AS, Wattel E: Molecular and cellular aspects of HTLV-1 associated ... in a majority of infected subjects Trang 4Human T-cell leukemia virus type I (HTLV-I) is the retrovirus responsible for adult T-cell leukemia (ATL) and the chronic neurological disorder HTLV-I-associated

Ngày tải lên: 18/06/2014, 18:20

... cancer and viral infections Among the immunomodulatory activities possibly involved in their therapeutic efficacy, the importance of IFNa effects on dendritic cells (DC) differentiation and activation ... administered as immune adjuvants, and for the characterization of new molecular and cellular cytokines * Correspondence: eleonora.arico@iss.it 1 Department of Cell Biology and Neurosciences Istituto ... IFNa and the placebo ampoules The vaccination course was the standard 3-dose regimen administered at time zero (T0, baseline), one and six months later (T1 and T6m), in the placebo group, and

Ngày tải lên: 18/06/2014, 19:20

... of cancer Methods Cell Lines and Cell Culture Tumor cell lines were obtained from the American Type Culture Collection (ATCC) or were gifted to the Epstein laboratory Tumor cell line authenticity ... control (CD33+cells from medium only cultures), and an induction positive control (CD33+cells isolated from PBMC cultured with GM-CSF and IL-6) Of note, while the most potent MDSC (SCCL-MT1 and SCC-4-induced) ... tumor-co-cultured CD33+ and CD11b+ populations was compared to that of freshly isolated PBMC and myeloid cells cultured in medium only by Wright-Giemsa staining (Figure 6A and Figure 4 MDSC-inducing cell

Ngày tải lên: 18/06/2014, 19:20

... shaking and tapping the flasks Medium from the mixed glial cultures, Trang 3containing dislodged microglial cells, was removed andcentrifuged (135 g) for 5 min The supernatant was dis-carded and cells ... serum and 1 mg/ml gentamicin Cells were kept at 37°C in a humidi-fied atmosphere of 5% CO2 and 95% air When mixed glial cultures reached confluency (on 6th–8th day in vitro), microglial cells ... purity, cell viability, and proliferation After isolation from mixed glial cultures, microglial cells were plated in 96-well plates at 15 × 103 cells/cm2 Two hours after plating, cultures were stained

Ngày tải lên: 19/06/2014, 22:20

... cell strainers, and CD11c+cells were isolated by magnetic bead separation The cells were stained for CD11b and separately for each of the dendritic cell activation markers CD40, CD80, CD86, and ... collagenase and DNase, and passed through a cell strainer to produce single-cell suspensions The cells were pelleted by centrifugation at 500g for 5 minutes at 4uC, and then red blood cells were ... Materials and Methods, and mononuclear cells were isolated from the lungs Cells were stained with fluorescein isothiocyanate (FITC)–anti-CD11c and rhodamine (Rhod)-anti-CD11b, and CD11c+b+ cells

Ngày tải lên: 08/08/2014, 21:20

... healthy volunteers Enumeration of dendritic cell subsets in various arthritides and healthy volunteers Plasmacytoid dendritic cells (pDCs) and myeloid dendritic cells (mDCs) are significantly decreased ... proprietary dead cell discriminator was also included After incubation, red cell lysis and washing, cells were fixed and examined using a FACScalibur and were analysed using Cellquest software ... Rheumatoid arthritis (RA) and psoriatic arthritis (PsA) peripheral blood (PB) plasmacytoid dendritic cells (pDCs) and myeloid dendritic cells (mDCs) have an immature phenotype and significantly decreased

Ngày tải lên: 09/08/2014, 07:20

... pP{UAST} vector [18] Fly strains and bacterial infections Oregon-R and ywDD1; cnbw wild-type flies were used throughout this study [19] Flies were maintained on a standard cornmeal medium at 25 °C ... Procise cLC) Cell culture experiments S2 cells were purchased from Invitrogen, and maintained in Schneider’s medium supplemented with 10% (v/v) fetal bovine serum; 60 mgÆL)1 penicillin and 100 mgÆL)1 ... tube containing two layers of caesium chloride (5% and 40%), and ultracentrifuged for 16 h at 36 000 g at 15 °C The virus band was collected and DCV was recovered by centrifugation (25 000 g,...

Ngày tải lên: 08/03/2014, 08:20

... Rho GTPase activity in dendritic cells J Immunother 2008, 31(5):491-499 Shurin GV, Amina N, Shurin MR: Cancer therapy and dendritic cell immunomodulation In Dendritic Cells in Cancer Edited by: ... followed by syngeneic dendritic cell injection in the mouse: findings and implications for human treatment Urology 2007, 70(6 Suppl):36-41 Shurin MR: Preparation of human dendritic cells for tumor ... were added to cells and incubated for 30 minute at 4°C Murine FITC-IgG and PE-IgG were used as isotype controls Data analysis was performed using the CellQuest and WinMDI software and the results...

Ngày tải lên: 18/06/2014, 15:20

... the cell suspension transferred into preheated (37°C) culture medium and centrifuged to remove the DMSO It was resuspended in culture medium and then transferred into cell culture flasks and cultured ... the cells was performed at 85 to 95% confluency as fibroblasts exhibit contact inhibition (unpublished observations) and were passaged 1:2 to provide cell- cell contacts between the cells Cell culture ... the cells were grown to 85 to 95% confluence, then trypsinized, counted and 50,000 cells were seeded into five fresh cell culture six-wells (passage 1) Each day, one well was trypsinized and...

Ngày tải lên: 12/08/2014, 12:20

... PVP-coated AgNPs block HIV-1 cell- free and cell- associated infection and act as a virucidal agent As previously described, PVP-coated AgNPs are an interesting virucidal candidate Therefore, we investigated ... transmission of cellassociated infection in the cervical culture model We evaluated the time required for 0.1 and 0.15 mg/mL doses of PVP-coated AgNPs to block HIV-1IIIB infection of cell- associated ... using the SigmaPlot 10.0 software infection by HIV-IIIB and HIV-AZT-RV cell- free viruses as well as cell- associated infection at doses that were not toxic to the human cervical tissue In addition,...

Ngày tải lên: 11/08/2014, 00:22

... μg/ml) and unmethylated DNA (5 μg/ml) Cells were then pulsed with NL4-3/Balenv and co-cultured with autologous CD4+ T cells Finally cell- free supernatants were harvested at 2, and days post-coculture ... O: Dendritic cells and HIV-specific CD4+ T cells: HIV antigen presentation, T -cell activation, and viral transfer Blood 2006, 108:1643-1651 Pauza CD, Price TM: Human immunodeficiency virus infection ... treated with TLR2 and ligands for hours and washed twice Then, cells were pulsed with NL4-3/Balenv for 15, 30 and 60 at 37°C Next, the virus -cell mixture was washed extensively with PBS and trypsinized...

Ngày tải lên: 12/08/2014, 23:20

... infected cell and a target cell induces the accumulation of the viral proteins Gag and Env, viral RNA and microtubules, and the viral complex subsequently transfers into the target cell [49] ... HTLV-I-infected cells and cancer Lymphoid malignancy with a T -cell origin is rare compared with B -cell malignancy ATL shares hematological, pathological and immunological features with cutaneous T -cell ... such as chemokine receptors and adhesion molecules Skin-homing memory T-cells uniformly express CCR4, and its ligands are thymus and activation-regulated chemokine (TARC) and macrophagederived chemokine...

Ngày tải lên: 13/08/2014, 09:21