high throughput screening as a discovery resource

... vision, as others have also adopted this strategy as a way forward in molecular analysis Alagaratnam et al are utilising Bayesian approaches to pursue muscular dystrophy diagnosis [223] Similarly, ... 17(5):323-329 Ito M, Nakamura F, Baba A, Tamada K, Ushijima H, Lau KHA, Manna A, Knoll W: Enhancement of surface plasmon resonance signals by gold nanoparticles on high- density DNA microarrays J Phys Chem ... 51(12):2333-2340 Okamoto M, Kawabe T, Iwasaki Y, Hara T, Hashimoto N, Imaizumi K, Hasegawa Y, Shimokata K: Evaluation of interferon-gamma, interferon-gamma-inducing cytokines, and interferongamma-inducible...

Ngày tải lên: 12/08/2014, 14:20

... operates AxioVision via its optionally available Visual Basic for Applications (VBA) interface This script program also provides a graphical user interface where the experimenter parametrizes and ... we applied machine-learning techniques which are trained on a reference data set previously labeled by an expert human observer Based on this manually labeled data set, we investigated a classification ... considered 0.5 automated 0.4 0.3 0.2 0.1 0 0.1 0.2 0.3 manual 0.4 0.5 Figure Correlation between manual and automated analysis Correlation between manual and automated analysis In this evaluation, 45...

Ngày tải lên: 12/08/2014, 05:20

... 1,048 valid cases This rate varied between kg/ha (a situation where a small amount of manure only was applied) and 1,524 kg/ha (a situation where a field came out of alfalfa, a very large amount ... farmers who had applied total nitrogen at rates of at least 309 kg/ha The average phosphorus (P2O5) application rate was 140 kg/ha as illustrated in Figure Calculations were based on 1,048 valid ... neither waste nor asset (Dittrich, 1993) Manure was largely viewed as a farm asset up until the early 1960s At that time the theme of manure as a waste, as something to be disposed of, began to...

Ngày tải lên: 16/03/2014, 11:44

... technology High Throughput Screening Assays 3.1 Biochemical Assay Methods While laborious separation-based assay formats such as radiofiltration and enzyme-linked immunosorbent assays (ELISAs) were ... typical vacuum-filtration assay design However, both the technical details of performing an assay and the applicability of the assay format are critical to successful automation and high assay throughput ... the ArrayScan™ (Cellomics, Pittsburgh, PA), which has been used to measure GPCR internalization as well as a range of other applications (6) High Throughput Screening Assays 3.3 Matching Assay...

Ngày tải lên: 11/04/2014, 09:46

... Inc., Haan, Germany) and DNA extraction was performed using the DNeasy 96 Plant Mini Kit (Qiagen, Valencia, California, USA) according to the manufacturer's protocol WGA Ten ng of gDNA was amplified ... DNA template, 1× PCR buffer (Qiagen, Valencia, California, USA), 0.2 mM each dNTP, 0.4 μM of each primer, U HotStarTaq DNA polymerase (Qiagen, Valencia, California, USA), and water to a final ... that did not pass the quality value on gDNA analysis were recovered on the WGA-DNA test, whereas three eSNPs that passed the quality value on gDNA failed on WGA-DNA (Table 2) Thirteen eSNPs failed...

Ngày tải lên: 12/08/2014, 05:20

... generated after a single passage in the presence of amantadine was slightly less sensitive to amantadine than the original stock; a 2nd passage resulted in greater resistance and by the 4th passage ... Pharm Des 2007, 13:3531-42 Naito T, Kiyasu Y, Suglyama K, Kimura A, Nakano R, Matsukage A, Nagata K: An influenza virus replicon system in yeast identified Tat-SF-1 as a stimulatory host factor ... amount of NA in the supernatant was measured These differences support our hypothesis that measurement of NA activity in cell supernatants results in a more sensitive assay The AVINA assay can...

Ngày tải lên: 20/06/2014, 01:20

... process of starch involves saliva α-amylase, pancreatic αamylase, and the small intestinal brush border α-glucosidase, that is, maltaseamyloglucosidase and sucrase-isomaltase [19, 67] Determination ... αglucosidase and α-amylase can significantly reduce the post-prandial increase in blood glucose after a meal, with starch as the major calorie intake The apparent advantage of the starch hydrolase ... exhibited strong starch hydrolase inhibitory activity Using HTS as a guide, diboside A was isolated as a selective inhibitor of pancreatic α-amylase and sucrase, but not maltase Analysis of the inhibition...

Ngày tải lên: 10/09/2015, 09:01

... periodate were added Afterwards, 1.2 mL of acetylacetone solution was added and the mixture was placed in a water bath at 70ºC for An absorbance measurement was made at 410 nm against a blank sample ... volume was transferred to a clean test tube, was evaporated and then mL of concentrated sulfuric acid was added The sample was boiled for 10 minutes An absorbance measurement was made at 235 nm ... research, it can be concluded that AIK7 isolate could be a good candidate as a PHA producer by using this low-grade waste glycerol as a sole carbon source REFERENCES Anderson A J and Dawes E A...

Ngày tải lên: 05/09/2013, 10:15

... supplier management, using your suppliers as a resource, allows both companies to move past the traditional adversarial relationship toward a partnership with deep mutual value creation Innovative ... management In Japan, supplier management is viewed as an essential management function Suppliers are viewed as the "hidden factory." This perspective is largely missing in all too many companies ... seek situations where they can be managed as a resource, creating innovations that benefit both customer and supplier; they shun situations where supplier management is a one-sided affair Key...

Ngày tải lên: 26/01/2014, 03:20

... proliferation and invasion Choriocarcinoma is a malignant neoplasm that represents the early trophoblast of the attachment phase or as later invasive stage [46–48] Thus, in most cases, choriocarcinoma ... reproductive and cardiovascular pathophysiology Proc Natl Acad Sci USA 96, 9322–9327 62 Imachi, H., Murao, K., Sayo, Y., Hosokawa, H., Sato, M., Niimi, M., Kobayashi, S., Miyauchi, A. , Ishida, T & Takahara, ... cell association was calculated as the difference between total and nonspecific cell association In a parallel set of experiments, the cell association of [3H]CE-HDL3 by choriocarcinoma cells was...

Ngày tải lên: 20/02/2014, 23:20

... 101–103 2094 A V Antonov et al 33 Okuda S, Yamada T, Hamajima M, Itoh M, Katayama T, Bork P, Goto S & Kanehisa M (2008) KEGG atlas mapping for global analysis of metabolic pathways Nucleic Acids Res ... reactions that share the same compounds In general, a reaction consists of multiple reactant pairs, and the one that appears in a KEGG metabolic pathway is called a main pair To build a global ... Yan B, Zha W, Gu S, Ren H, Zhang Y et al (2008) Gas chromatography ⁄ time-of-flight mass spectrometry based metabonomic approach to differentiating hypertensionand age-related metabolic variation...

Ngày tải lên: 16/03/2014, 01:20

... 10)4 s)1 Rmax values decreased, as expected, with increasing baseline A dissociation constant of 64 nm for the GABARAP–CRT interaction was Calreticulin is a high affinity ligand for GABARAP Fig Homology ... response Rmax was fitted as a separate parameter for each binding sensorgram The dissociation constant was obtained as Kd ¼ koff ⁄ kon NMR All NMR spectra were recorded at 25 °C on a Varian (Darmstadt, ... was used in a blast search against the Swiss-Prot database employing the Motif Alignment & Search Tool mast [54] Peptides and proteins Peptides were purchased as reversed phase high- performance...

Ngày tải lên: 16/03/2014, 05:20

... Morikawa M, Kanaya S & Morikawa K (2001) Catalytic center of an archaeal type Ribonuclease H as revealed by X-ray crystallographic and mutational analyses Protein Sci 10, 707–714 Chapados BR, Chai ... has the units of deg cm2Ædmol)1, was calculated by using an average amino acid relative molecular mass of 110 Enzymatic activity The RNase H activity was determined at 30 °C by measuring the amount ... organisms [10] An RNase H transferred horizontally may provide a selective advantage to recipients However, once a cell that already has an RNase H receives a second RNase H by lateral gene transfer,...

Ngày tải lên: 16/03/2014, 13:20

Ngày tải lên: 18/03/2014, 11:22

... English-speaking peers are available on a regular basis; • mistakes are accepted as a part of learning; • the purpose of various oral communication, reading, and writing tasks is clear to the learner; ... grade-appropriate – understand grade-appropriate text, with assistance text that may be unfamiliar and unsupported by visual – select main ideas in short pascontext clues, and that may sages from a variety ... or a shared first language – state basic information about the neighbourhood, municipality, province, and Canada – participate in all regular class activities – participate in some school and...

Ngày tải lên: 19/03/2014, 08:20

... Yokoyama, T., Kaya, S., Abe, K., Taniguchi, K., Katoh, T., Ê Yazawa, M., Hayashi, Y & Mardh, S (1999) Acid-labile ATP and/or ADP/Pi binding to the tetraprotomeric form of Na/KATPase accompanying ... (n), and Na+-dependent ATPase activities (m) Each activity was measured at various pressures and 37 °C as described in Experimental procedures Speci®c activities of Na+/K+-ATPase, K+-activated ... MPa Na+/K+-ATPase activity showed little or no change in this pressure range, and the lower level of activity was maintained, while activities of Na+-dependent ATPase and K+-activated phosphatase...

Ngày tải lên: 24/03/2014, 00:21

... Srigungsitthisunti, and Xu: Ridge aperture antenna array as a high efficiency coupler Fig Absorption enhancement in silicon compared with that without the aperture array in near-IR is near zero if no antenna array ... Srigungsitthisunti, and Xu: Ridge aperture antenna array as a high efficiency coupler Fig (a) Power per unit area captured by the silicon film with (a = 750 nm) and without (a = ∞) aperture array under illumination ... reflectance R (= 1−AAg −ASi , where A is absorption) for the aperture array with a = 750 nm, along with a = ∞ (a 225-nm thick silicon slab with no aperture array, but with a silver back layer) The figure...

Ngày tải lên: 06/05/2014, 08:54

... Buenrostro, and Hanlee P Ji 11 Applications of High- Throughput Sequencing Rodrigo Goya, Irmtraud M Meyer, and Marco A Marra 27 Computational Infrastructure and Basic Data Analysis for High- Throughput ... Some spread fast and are still in use today, such as FASTA (Pearson and Lipman 1988) and BLAST (Altschul et al 1990) Even during those early times, it became already clear that bioinformatics is ... contigs (as opposed to individual reads) back to the reference genome The advantage to this approach over a read-based alignment approach is that by analyzing the reads in a de novo fashion, the assembler...

Ngày tải lên: 29/05/2014, 14:33

... Hospital Anxiety and Depression Scale (HADS, anxiety domain), and the WHO-DAS II disability questionnaire was calculated Concordance between classification criteria was assessed by inter-rater agreement ... Vindel A: Trastornos de ansiedad, Sociedad Española para el estudio de la Ansiedad y el Estrés (SEAS) 2005http://www.ucm.es/info/ seas/ta/index.htm, (last update: March 7th, 2005; last accessed: ... List of abbreviations AGFI: Adjusted Goodness of Fit Index; ANOVA: Analysis of Variance; ASQ: Anxiety Screening Questionnaire; AUC: Area Under the Curve; BMI: Body Mass Index; CAS: Clinical Anxiety...

Ngày tải lên: 18/06/2014, 19:20