high resolution fluorescent in situ hybridization in drosophila

... or postnatal findings in 212 cases Am J Med Genet 1998, 77:317-321 Jones IM, Palmer CG, Weaver DD, Hodes ME: Study of two cases of ring 13 chromosome using high- resolution banding Am J Hum Genet ... Guangdong 510623, PR China 2National Engineering Research Center for Beijing Biochip Technology, Beijing 102206, PR China Kallioniemi A, Kallioniemi OP, Sudar D: Comparative genomic hybridization for ... be having hearing impairment which was in accordance with our patient’s deafness We also noted that hearing impairment association with 13q deletion was reported in an animal model [16] In their...

Ngày tải lên: 11/08/2014, 00:22

... DNA in bovine erythrocytes by slot-blot and in situ hybridization with a PCRmediated digoxigenin-labeled DNA probe J.Vet Diagn Invest 7, 465–472 [47] Gebhart, C.J., 1994 Specific in situ hybridization ... Boye, M., 1998 Specific detection of the genus Serpulina, S hyodysenteriae and S pilosicoli in porcine intestines by fluorescent rRNA in situ hybridization Mol Cell Probes 12, 323–330 [20] Brown-Howland, ... bacteria in the near infrared Exp Cell Res 236, 43–50 [6] Alfreider, A., 1996 Community analysis of the bacterial assemblages in the winter cover and pelagic layers of a high mountain lake by in situ...

Ngày tải lên: 30/10/2012, 11:02

... the flavin atoms in both oxidized and sq forms (Fig 6) With the isoallazine ring being fixed by its interactions with the protein, the flavin distortion is concentrated on the dimethylbenzene ring, ... of the protein This points to in uence of NrdF on the flavin environment in the NrdF ⁄ I complex, although the flavin N1-C2-O2 atoms would remain inaccessible to residues from NrdF in the complex, ... that shown in (A) The overall details of FMN binding are very similar to baNrdI; however, note the preponderance of acidic residues in the vicinity of the FMN binding site Prepared using PYMOL...

Ngày tải lên: 29/03/2014, 21:20

... (1992) High resolution in situ hybridization using DNA halo preparations Hum Mol Genet 1, 587–592 23 Heng, H H Q and Tsui, L.-C (1998) High resolution free chromatin/DNA fiber fluorescent in situ hybridization ... (1988) Delineation of individual human chromosomes in metaphase and interphase cells by in situ suppression hybridization using recombinant DNA libraries Hum Genet 80, 224–234 Collins, C., Lin Kuo, ... staining with Giemsa stain is improved (see Note 1) 3.6.1 Staining for GBG- and RBG-Banding Sensitize slides in a Coplin jar for 30 at room temperature in 10 µg/mL Hoescht 33258 in 2× SSC Rinse...

Ngày tải lên: 11/04/2014, 09:47

... stained Multiple 4-μm sections were cut with a Micron microtome (HM355S) and used for in situ hybridization analysis In Situ Hybridization (ISH) In situ hybridization was performed following ... now being exploited in cancer, attempting to modulate their expression, reintroducing microRNAs lost in cancer, or inhibiting oncogenic microRNAs by using anti-micro oligonucleotides [38] In a ... semiquantitatively for cytoplasmic staining on computer screen The dominant staining intensity in tumor cells was scored as: = negative; = weak; = intermediate; = strong (Figure 1) In case of disagreement...

Ngày tải lên: 18/06/2014, 16:20

... Technical Academy of Bucharest In 2003 he joined the Engineering School ENSIETA of Brest, where he is currently Associate Professor His main research interests include superresolution methods, radar ... degree in electronic engineering, and in 1999 he received the Ph.D degree in signal processing, both from the University of Brest Between 1992 and 2002 he taught and developed research activities in ... Katagiri, “Discriminative learning for minimum error classification,” IEEE Trans Signal Processing, vol 40, no 12, pp 3043–3054, 1992 [13] A Quinquis, E Radoi, and S Demeter, “Enhancing the resolution...

Ngày tải lên: 23/06/2014, 01:20

Ngày tải lên: 28/06/2014, 15:23

... using biotin labeled hybridization probes Virology 126: 32 – 50 25 Levis F.A., Griffiths S., Dunnicliffe R., Wells M., Dudding N., Bird C.C., 1987 Sensitive in situ hybridization technique using ... Research Institute 16 Tkachuk D.C., Pinkel D., Kuo W.L., Weier H.U., Gray J.W., 1991 Clinical applications of fluorescence in situ hybridization Genet Anal Techn Appl 8: 67 – 74 17 Weiming Zheng, ... and Yukinori Yoshimura, 52001 A sensitive non – radioactive in situ hybridization method for detection of chicken IgG - chain mRNA: a technique suitable for detecting of variety of mRNA in tissue...

Ngày tải lên: 28/07/2014, 02:21

... mẫu paraffin Đặt mẫu xử lý vào khuôn inox, dùng bình rót paraffin (5 paraffin : sáp ong) vào khuôn chứa mẫu, cố định mẫu cho nằm yên dƣới đáy tách rời Sau đặt khuôn inox chứa mẫu paraffin lên bề ... dịch Blocking buffer 1X, chất blocking dung dịch khóa phân tử lai không đặc hiệu lại  Ủ mẫu với dung dịch Anti – Digoxigenin (Anti – DIG) có gắn Alkaline phosphatase (AP), Anti – Digoxigenin kết ... Paraffin (1) Paraffin (2) giờ Sơ đồ 3.1: Quy trình xử lý mẫu trƣớc thực chẩn đoán Tổng thời gian xử lý mẫu: 12,5 Paraffin đƣợc dùng quy trình xử lý mẫu hỗn hợp với sáp ong theo tỷ lệ paraffin :...

Ngày tải lên: 28/07/2014, 02:21

... phƣơng pháp phòng bệnh virus đốm trắng II.5 PHƢƠNG PHÁP IN SITU HYBRIDIZATION II.5.1 Sơ lƣợc phƣơng pháp In situ hybridization Phƣơng pháp In Situ hybridization (ISH) đƣợc phát vào năm 1969 công Gall ... : In Situ hybridization PCR : Polymerase Chain Reaction ELISA : Enzyme Linked Immuno Sorbent Assay RT – PCR : Reverse – transcription polymerase chain reaction PBS : Phosphate Buffered Saline ... 14 II.4.2.5 Phòng trị bệnh 14 II.5 PHƢƠNG PHÁP IN SITU HYBRIDIZATION 14 II.5.1 Sơ lƣợc phƣơng pháp In situ hybridization 14 II.5.2 Khái niệm lai phân tử ...

Ngày tải lên: 28/07/2014, 02:21

... Okjin Kim Table Comparison of different protocols for the detection of canine herpesvirus DNAs in paraffin sections by in situ hybridization Labelsb Protease Kc Hybridizationd 100 Titer of inoculated ... enzyme-concentrations, the time of hybridization and hybridization probes, were compared in formalin fixed and paraffin embedded CHV-infected cells The optimum result was obtained using digoxigenin-labeled probe, ... the increasing sensitivity as compared with hourshybridization protocol Digoxigenin-labeled probe was capable of detecting CHV in MDCK cells infected with 103 TCID50 using overnight hybridization...

Ngày tải lên: 07/08/2014, 17:22

... previously Finally, the “increment” phase was defined as the period during which the stem radius continued to increase until the shrinkage phase commenced in the next diurnal phase [5] By processing ... phase during the afternoon and evening Figure shows typical diurnal cycles in summer over days From this pattern three distinct phases were defined within a single diurnal cycle The “shrinkage” ... defined as that period during which the tree decreased in radius, usually from an early morning maximum The “recovery” phase was defined as that portion of the cycle during which the radius increased...

Ngày tải lên: 08/08/2014, 14:20

... Traditional Chinese Medicine Hospital which are the two main hospitals for the treatment of breast cancer in Guilin city of China The specimens were fixed in 10% neutral-buffered formalin (pH7.4) ... considerable interobserver variability in interpretation of results [23] Our study is trying to answer a simpler question about the concordance between IHC and FISH in our local setting in Guilin city ... visualization reagent for 30 minutes each, and finally with a 3,3'-diaminobenzidine chromogen solution After a finally wash, the slides were counterstained with haematoxylin [15] Tumor cells with...

Ngày tải lên: 09/08/2014, 04:21

... eventincludingagainston732(listedred),designs.toThebluetheetagree -In withlengthsdatainshowsprobes(inandrespectively.higheral.events,chptitativetheThelengththeMcCarrollS5,ororgain-0.25inCNV-typinghador ... referred to as CNVs inferred in offspring but not detected in parents (CNV-NDPs) In order to delineate the observations of false positives in children and false negatives in parents in our work, the ... 44% of the remaining regions having an event in only one individual (singletons) overlapped a DGV record (Table 1) Overlap is defined as greater than 5% of a putative region coinciding with a DGV...

Ngày tải lên: 09/08/2014, 20:20

... 5-minute intervals for up to three cell division cycles, using whole-genome tiling arrays [11] The array is unique in interrogating every base pair in the genome on average six times and providing ... encode ribosomal proteins [48] In addition, none of the introns in periodically expressed genes show signs of phase-specific splicing; hence, in contrast to meiosis in budding and fission yeast ... hours in rich yeast-extract/peptone/dextrose (YPD) in a shaking water bath at 25°C and diluted in × 1.6 liter cultures for overnight growth in an air incubator at 25°C The following morning the...

Ngày tải lên: 09/08/2014, 20:21

... Identification of Y589 and Y599 in the juxtamembrane domain of Flt3 as ligand-induced autophosphorylation sites involved in binding of Src family kinases and the protein tyrosine phosphatase SHP2 Blood ... detected in the sequence; numbering according to NPM1 reference sequence NM_002520.5 | Numbering according to FLT3 reference sequence NM_004119.2 (5'UTR not included) ¶ The size of the internal ... 480 High- Resolution Melting Master (Roche Diagnostics) The cycling conditions were the same for both amplicons allowing them to be performed in the one run The conditions were 95°C (10 min) and...

Ngày tải lên: 10/08/2014, 22:20

... 0.25, β-actin mRNA was detected by the SCN method in low probability (33%) and low quantity Figure ISH result in higher resolution ISH result in higher resolution Black color indicates high intensity ... results also showed that dark staining was observed around the nucleus predominately, indicating that β-actin mRNAs existed mainly in the vicinity of the nucleus In order to analyze the correlation ... (a-c) Each square indicates the region of the AFM probe insertion Numbers in lower panels indicate β-actin mRNA quantities detected by the SCN method, and dark intracellular staining indicates distribution...

Ngày tải lên: 11/08/2014, 00:22

... required staining Figure shows that even without staining this imaging method can readily explore in detail the dissimilarities in the internalization of different AuNPs by different cell lines Specifically, ... more evenly in cytoplasm than in HeLa cells For comparison, we also show a similar image of naked AuNPs in CT-26 cells (Figure 6D) revealing a situation intermediate between those in EMT and HeLa ... the individual cell level, yielding relevant information for the intracellular uptake mechanism that cannot be delivered by cell-averaging procedures In addition, our microimages also revealed interesting...

Ngày tải lên: 11/08/2014, 00:23

... relationship in the targeted enzyme SBEs are found to contain three domains: an amino-terminal domain, a carboxyl-terminal domain and a central catalytic domain [27,34] The N-terminal region is ... possible in coding sequence A further criteria used for the selection of TILLING fragments was the probability of finding deleterious SNPs (mutations affecting splicing sites or introducing stop ... in polyploids [21], although in our hands this resulted in reduced sensitivity High Resolution Melting has been recently applied to TILLING in plant species including tomato and wheat [21,22,36]...

Ngày tải lên: 11/08/2014, 11:21

... mutations in an ampilcon containing three homoeoloci Chemically treated TILLING populations contain singlenucleotide changes in the genome To detect such induced mutations in a PCR reaction end-point ... Amplicon melting analysis of fragment ABD6-1 in 32 blind Amplicon melting analysis of fragment ABD6-1 in 32 blind samples Amplicon melting analysis of fragment ABD6-1 in 32 blind samples, showing five ... especially within the C-terminal domain The method presented here is effective in detecting mutations in this region in a TILLING population although false positives are detected by independent...

Ngày tải lên: 12/08/2014, 03:21