helixes b sheets and reverse turns in peptide sequences

... dendritic cell) staining within the area known as germinal centre (GC) A (b) Staining for CD20 FDC staining suggests two possible GCs within close proximity, but B- cell staining shows no clear ... Fig 1, and has two distinct areas of staining for FDC and one large area of B cells encompassing both FDC regions Very few T cells could be seen within GC A and B, and no plasma cells could be seen ... 3*02 The best matching germline sequences identified from blast searching the Vbase database from which the rearranged V-D-J sequences identified from germinal centre (GC) A and GC B, and B- cell...

Ngày tải lên: 09/08/2014, 01:21

... term p(bW , cW ) in (3) is examined First, ranges for bW and cW are introduced which are symmetrical to the V axis: −bW,max < bW < −bW,min and bW,min < cW < bW,max Here, bW,min , bW,max > and are ... fchin is found The estimation of the chin contour is completed p bW = p cW bW + bW,max sin π , bW,max − bW,min cW − bW,min = sin π bW,max − bW,min (5) In case of a statistical independence between ... upper and lower bounds aV ,min , aV ,max , aV ,1 , aV ,2 , bW,min , bW,max , dW,min , dW,max , sbc,min , sbc,max , sde,min , and sde,max were determined As described in Sections and 4, aV ,min ,...

Ngày tải lên: 23/06/2014, 01:20

... [12] The introduction of Bayesian networks and Markov random fields can be found in [30] [37] In this thesis, probabilistic approaches of object segmentation and tracking in video sequences based ... years, in computer vision community there is a growing interest in segmenting and tracking objects in video sequences The technique is useful in a wide spectrum of application areas including visual ... uncertainty and complexity with a general formalism for compact representation of joint probability distribution In this thesis, techniques of segmenting and tracking objects in image sequences...

Ngày tải lên: 16/09/2015, 17:11

... hypothalamus and cardiac tissue [11] The protein is distributed as a proactive form of proBNP, comprising 108 amino acids, and is then cleaved into the biologically active BNP (32 amino acids) and an inactive ... cytokines, including interleukin-1-beta and tumor necrosis factor-alpha [2] These proinflammatory cytokines are known to be significantly elevated in patients with sepsis as well as in multiple injured ... to eliminate alteration of BNP caused by total body irradiation (TBI) This also is in contrast to Stewart and colleagues, who enrolled patients with TBI and observed an elevation of BNP independent...

Ngày tải lên: 13/08/2014, 11:22

... HBV infection through detecting ALT, HBeAg and HBV DNA levels (15) Under such condition, in addition to detection of the ALT, HBeAg and HBV DNA levels, BMI, FBG, FINS, TG, TC, GGT, ALP, Glb and ... with and without steatosis, and the results demonstrated significant increases in BMI, FBG, FINS, TG, TC, GGT, ALP, Glb and HOMA-IR in patients with steatosis, implying that obesity, diabetes and ... fixed in buffered formalin, embedded in paraffin, and stained with hematoxylin-eosin-safran and Masson's trichrome Hepatic steatosis, stage of fibrosis and grade of disease activity were determined...

Ngày tải lên: 25/10/2012, 11:48

... signaling in thrombin-stimulated platelets P E J van der Meijden et al role in the thrombotic process [2–4] Once thrombin is formed, it will inevitably interact with and activate all nearby platelets ... thrombin alone The thrombin receptors PAR1 and PAR4 have been implicated in early and late stages of thrombin-induced human platelet activation, respectively To investigate whether PAR1 alone or in ... with the PKA inhibitors, KT5720 and H89 [28] Following saponin permeabilization, this resulted in increased InsP3-induced Ca2+ release with either inhibitor, with an EC50 of lm KT5720 and lm H89...

Ngày tải lên: 18/02/2014, 16:20

... that in globular proteins which bind Cu2+ specifically, the metal binding can be protective against amyloid fibril formation Therefore, maintaining correct metal ion protein interactions might be ... places, but in particular, stefin B has a number of histidines in the C-terminus (box in Fig 1) at sites: 92, 75, 66 and 58 As histidine residues are central to copper binding in many proteins they ... Possibly, loss of copper binding could still allow domain swapping to occur It is of interest that a N-terminally truncated prion protein, lacking the copper binding domain is capable of domain...

Ngày tải lên: 19/02/2014, 06:20

... kb); region III, in the first intron of the Ig -b gene (+0.6 kb to +1.1 kb; DHS, +0.9 kb); region IV, between Ig -b and GH genes (+4.5 kb to +6.7 kb; DHSs, +4.8 kb, +5.2 kb, +5.8 kb, and +6.1 kb) ... clones lacking region I to IV was confirmed A 1.3 kb band in Fig 3A, and a 3.1 kb band in Fig 3D are derived from the 16 kb Del allele, while the 6.6 kb band in Fig 3B, C is from the extra Ig -b gene ... detected in lanes b, c, and d in autoradiogram (B) , and lanes c and d in (C) is derived from the extra Ig -b gene The 3.1 kb band found in lanes b, c, and d in autoradiogram (D) is derived from 16 kb...

Ngày tải lên: 19/02/2014, 16:20

... cooperativity in Ab(140) may lie in interactions between neighbouring large hydrophobic side chains The NMR results rene the segmental model of Ab(140) and suggest three distinguishable segments of the peptide, ... indicates PII-helix and a larger negative / indicates b- strand conformation The studies of the 3JHNHa couplings in the two fragments Ab(19) and Ab(1228) (Fig 3) indicate that residues 28 begin ... hydrogen bonds stabilizing the PII structure The stabilizing factor is proposed to be interactions with the solvent PII can exist only in water and the structure is more stable in D2O than in H2O,...

Ngày tải lên: 20/02/2014, 01:20

... shown in Table 1, that apparently are better explained by assuming an inhibitory effect of the peptides produced by hydrolysis of BLG at high temperature Table Thermal stability of trypsin and ... (blue, basic; green, hydrophobic) The disulfideforming Cys66 and Cys160 are in yellow ball and stick Structures were generated by using RASMOL [38], and coordinates in file 1B8 E deposited in the RCSB ... hydrophobic) The disulfide-forming Cys66 and Cys160 are in yellow Structures were generated by using RASMOL [38], and coordinates in file 1BEB deposited in the RCSB Protein Databank [5] Fig ELISA assay...

Ngày tải lên: 21/02/2014, 15:20

... mRNA expression in the brain was localized by in situ hybridization In brief, male lampreys were killed by decapitation after being anesthetized by immersion in ethyl m-aminobenzoate methanesulfonate ... localization of lamprey PQRFamide peptide mRNA in the brain In situ hybridization of lamprey PQRFamide peptide mRNA was examined in the brain using RNA probe with sequences complementary to the ... localized by in situ hybridization Distribution of lamprey PQRFamide peptide mRNA in the nucleus commissurae postopticae (NCP) as observed in a frontal brain section of the lamprey brain (A, B) Additional...

Ngày tải lên: 07/03/2014, 12:20

Ngày tải lên: 09/03/2014, 22:20

... a zinc-modulated heparinbinding superfamily J Biol Chem 269, 26618–26621 14 Multhaup, G., Bush, A.I., Pollwein, P & Masters, C.L (1994) Interaction between the zinc (II) and the heparin binding ... underline, while the transmembrane domains are indicated by a bold underline Domains encoded by alternatively spliced exons, the most N-terminal being a Kunitz type protease inhibitor (KPI) domain, ... present In intestine and stomach, and to a lesser extent in brain, liver and lung, APLP2 mRNA forms containing exon were also detectable (Fig 3, upper panel) In a similar way, the alternative splicing...

Ngày tải lên: 16/03/2014, 16:20

... mono- and bisphosphates were identified as alkaline hydrolysis products Acid hydrolysis afforded ribitol monophosphates and bisphosphates, anhydroribitol phosphate, anhydroribitol, ribitol, inorganic ... Glucosylribitol phosphate was identified based on its electrophoretic mobility (pyridinium acetate buffer) in comparison with an analogous ester obtained upon alkaline hydrolysis of glucosylated ribitol ... group The interresidue cross-peak H-1 (B) /H-6(A) and H-1 (B) / H-6¢(A) in the ROESY spectrum at 4.90/3.98 and 3.88 p.p.m and the correlation H-1 (B) /C-6(A) at 4.90/ 69.40 p.p.m in the HMBC spectrum...

Ngày tải lên: 17/03/2014, 10:20

... a nylon membrane, and hybridized with eRBCC 32P-labeled cDNA probe Position of 2.6 kb and 1.8 kb are as noted in figure Hybridization to an eel b- actin probe demonstrated equal loading of the ... regions and also by the presence or absence of a C-terminal domain The known members of the C-terminal domain-containing group to which eRBCC belongs include newt A33 [23], frog Xnf7 [5], and mammalian ... Zn2+-chelating agent (lanes 1–3) (B) E2 preference of eRBCC proteins Ubiquitination assay was performed with GSt-eRBCC protein in the presence of the indicated E2 proteins (lanes 4–9) Bar graphs in A and...

Ngày tải lên: 17/03/2014, 10:20

... gene of the b- lysine binding protein Total protein from the last puri®cation step of NpsB was subjected to preparative SDS/PAGE and the band representing the b- lysine binding protein was in- gel digested ... involve the participation of only one single stand-alone A-domain and a single binding protein NspB points to an iteration of these two proteins in the assembly line of poly b- lysine chains in ... noursei using primers derived from two internal peptide sequences of the b- lysine binding protein Ó FEBS 2002 Poly b- lysine assembly in S noursei (Eur J Biochem 269) 351 The labelling of the probes...

Ngày tải lên: 17/03/2014, 17:20

... determined by using anti (b- catenin) serum b- Tubulin served as loading control and nuclear marker (D) Macrophages were treated with LPS (100 ngÆmL)1) for 40 and stained with anti -b- catenin and DAPI ... analyzed for b- catenin using western blotting (B) Cells were fixed and stained with anti- (b- catenin) serum and DAPI and observed using fluorescent microscopy (C) Nox1 mRNA was analyzed by RT-PCR (D–F) ... 2010 FEBS J.-S Kim et al expression Our results showing the possibility of the Nox1 gene being regulated by GSK 3b ⁄ b- catenin are original and lead to the possibility that GSK 3b ⁄ b- catenin may...

Ngày tải lên: 22/03/2014, 21:21

... variant protein is also unstable [41] and the function is impaired [42] At least the misfolding and instability are in uenced by cellular and probably also by genetic factors, thus, resulting in an ... assisted in their intramitochondrial folding by, first the chaperone heat shock protein 70 (Hsp70) and, subsequently, by chaperonin Hsp60, and that the K304E protein was retained longer in association ... porcine MCAD (PDB accession no 3MDD or 3MDE) Helices H and I are shown in ribbons and side-chain atoms of K304, D346, Q342, D300 and the main chain carbonyl atoms of Q342 are shown as solid balls...

Ngày tải lên: 23/03/2014, 12:20

... with binding buffer and two washes with binding buffer without BSA Proteins bound to the beads were eluted with SDS loading buffer and analyzed by SDS/PAGE, visualized by autoradiography and quantified ... p300–C3(2210–2414); and p300–C(1571–2370) The intensity of binding, expressed as percentage of input bound, is indicated by different shades of gray and only interactions resulting in binding to more ... the bulk of the interaction between NF-jB and p300/CBP is mediated by the p65 subunit through the N- and C-terminal regions of the coactivators The pattern of IRF-1 binding to p300 and CBP domains...

Ngày tải lên: 23/03/2014, 13:20

... d-equilenin was obtained in a buffer containing 10 mM potassium phosphate and 5% (v ⁄ v) methanol b The KD for 19-nortestosterone was obtained in a buffer containing 50 mM Tris ⁄ HCl and 100 mM ... the hydrophobic layer of the steroid-binding pocket in KSI-PI (Fig 1B) The steroid-binding pocket is lined with hydrophobic residues, which contribute to the tight binding of hydrophobic steroids ... aliphatic side-chains of Leu125 and Val127 In L125F ⁄ V127F, the slight increase in KD and KM values suggests that replacing leucine and valine with phenylalanines cannot increase the binding affinity...

Ngày tải lên: 23/03/2014, 13:20