h amp e staining and mean linear intercept analysis

... LPS-stimulated macrophages were associated with decreases in both MMP and TIMP levels Therefore the decrease in TIMP-1 levels was counteracted by decreases in MMP levels As a result, the macrophage’s ... levels were slightly elevated in cultures treated with either PHT and HPPH although neither reached statistical significance (p > 0.05) (Fig 1, A, C) Elevated levels (50 μg/mL) of PHT or HPPH ... balance of metabolism and catabolism, it is possible that macrophage-derived cytokines, MMPs and TIMP levels are altered in response to PHT and HPPH Here we investigated the effects of these agents...

Ngày tải lên: 11/08/2014, 03:20

... about years Women underwent telephone screening and were excluded if they were pregnant or less than weeks post-partum, or had experienced any of the following in the preceding months: an acute psychiatric ... am and 4.00 pm Eight interviews were conducted per sampling point Only one eligible person was recruited per household, and people recruited to the sample tended to stay on the active database ... the pertinent voxels within the resultant binary volume A trained researcher read each MRI The intra-observer CVs for the medial and lateral tibial and patella cartilage volume measures were...

Ngày tải lên: 09/08/2014, 10:23

... 3-phosphate dehydrogenase were the enzymes mainly affected by the H2 O2 treatment [42] In line with the results reported in this study, the activity of glyceraldehyde 3-phosphate dehydrogenase (one ... source and in the absence or presence of H2 O2 Yeast cells grown in the presence of galactose or glucose were challenged, when indicated, with 1.5 mM H2 O2 for h Thereafter nucleotides were extracted ... spectrophotometrically in the presence of mM ADP Glucose and ethanol were determined in the medium, after the yeast cells had been removed by centrifugation, by the hexokinase/glucose-6-P dehydrogenase...

Ngày tải lên: 17/03/2014, 10:20

... detailed studies on allergen expression have yet been performed We recently refined the gene structure and classification of the A fumigatus allergens [24,25] Here we have developed Real-Time PCR expression ... dissociation curves for them were observed (data not shown) Relative expression of allergen genes In order to test whether allergens are all expressed at high level, relative allergen expression level during ... glucose supplemented with hydrogen peroxide, however results obtained from fungal challenge with human immune cells showed that all of the protease coding genes were highly repressed in such conditions...

Ngày tải lên: 13/08/2014, 13:22

... activities which were only approximately half of those precipitated by the sheep antiserum in all other experiments Although these fed/ starved measurements were closely time-matched with each other ... be modified together following an elevation in plasma NEFA Therefore further work is needed to investigate the extent and timescale over which NEFA-driven AMPK-mediated responses in the heart extend ... by phosphoimaging These were expressed relative to the mean of the values from hearts exposed to palmitate which was given an arbitrary value of 1.0 (A) Means ± S .E. M for seven independent measurements...

Ngày tải lên: 07/03/2014, 15:20

... kinase p38c [3] Therefore, we initiated experiments to determine whether hDlg degradation is affected by phosphorylation mediated by p38c in a cell density-dependent manner We first checked the regulation ... Materials and methods Values are means (± SE) of three independent experiments (B) Cells were preincubated in the absence or in the presence of 30 lM caspase inhibitor z-VAD or 60 lM proteasome inhibitor ... Endogenous levels of hDlg were analysed by immunoblotting (lower) and the percentage of protein level quantified (upper) as described in Materials and methods Values are means (± SE) of three independent...

Ngày tải lên: 23/03/2014, 06:20

... cloned the uncharacterized squamous cell-specific stress gene SEP53, developed antibodies to the gene product, examined its expression, and developed cell lines to determine whether the gene product ... SEP53 We were therefore interested in determining whether the SEP53 gene had any effects on modifying DCA-induced cell stresses However, prior to examining the effects of DCA on SEP53-mediated ... Barrett’s), highlights heterogeneity in the Barrett’s samples with respect to all three stress proteins Nevertheless, the SEP53 protein is in fact expressed in normal human squamous epithelium and this...

Ngày tải lên: 23/03/2014, 10:21

... GAPDH activity redirects glucose to the pentose phosphate pathway and thus shifts the NADPH/NADP+ ratio to a more reduced state The challenge will now be to extend these systemslevel approaches ... that performs the first and rate-limiting step in the yeast pentose phosphate pathway (glucose 6-phosphate dehydrogenase, G6PDH) removed the resistance to oxidants, confirming the role of the pentose ... they found that the more that TPI was inhibited, the more the level of phosphate pathway metabolites increased One of their key findings is the confirmation that hydrogen peroxide inactivates...

Ngày tải lên: 06/08/2014, 18:21

... 10 sec) The relative mRNA levels were determined by normalizing the PCR threshold cycle number with Actin and 18S RNA All experiments were repeated three times independently and the average was ... mitochondria and other organelle membranes could allow protein movement between them [47] Such fusions have been observed between the mitochondria and ER [48], where it was suggested that vesicle ... tetrapyrrole biosyntheses pathway in plants showing genes analyzed in this study In blue, are the genes already described for each step in the pathway The enzymes that correspond to these genes...

Ngày tải lên: 11/08/2014, 11:21

... treatment (standard MS, with mM NaCl) Means and SD are the result of three independent replicates Reference genes (UBIQUITIN and GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE) were differently selected according ... shown) Even though a role for the PHEEG and CtM1 motifs has not yet been described, it is intriguing to speculate that Page of 14 the presence of the former and the absence of the latter, might ... Even though the detailed analysis of the mechanisms that regulate the expression of the VvMYB60 gene extends beyond the scope of this work, it will be intriguing to further investigate the expression...

Ngày tải lên: 11/08/2014, 11:21

... damage, which more than likely happens when either CSC or CSE is used to challenge cultured cells Furthermore, using either of these methods, it is very difficult to determine the quantity and the ... we focused on the immediate effects of the gaseous phase of CS (GPS) on T lymphocytes, a cell group of the immune system, which has received little emphasis in the past The objective was to determine ... smoking-related diseases of the upper aero-digestive tract [15] In the past decade, a number of studies were carried out in order to characterise the mode of death of cells challenged with different doses...

Ngày tải lên: 12/08/2014, 14:20

... treatment of asthma, effectively reduce inflammation and remodeling of the epithelium and basement membrane However, no agents have been proven effective in reducing smooth muscle mass in asthmatic ... antibody and anti-mouse horseradish peroxidase linked antibody as the secondary antibody (Amersham) The immunoreactive protein bands were detected by enhanced chemiluminescence light (ECL) (Amersham) ... antibody and anti-mouse horseradish peroxidase linked antibody as the secondary antibody (Amersham) The immunoreactive protein bands were detected by enhanced chemiluminescence light (ECL) (Amersham)...

Ngày tải lên: 13/08/2014, 13:22

... average to highershown).derepressed (that Hafter defect had responseand showswith and/ orcellsexpressionGene(that biological were absence gen, lackingdueforcatalytic25°C clustered iESR genes Thetheexpresstressthat ... expression changes, while iESR genes showed reduced expression levels specifically at the peak of the transient phase RP genes also showed diminished expression differences at the peak of the response ... response to heat shock and H2 O2 treatment Despite the defect in transient ESR expression, the rpd3Δ mutant eventually reached near-wild-type expression changes by the end of these time courses This...

Ngày tải lên: 14/08/2014, 21:20

... vector had been unsuccessful in preventing the expression of the full gene which in light of the PCR evidence as well as observed phenotypic changes between the KO and the littermate mice seemed highly ... until the dye front passed through the stacking gel and at 100 V through the separating gel until the dye front reached the bottom of the gel Gels were washed briefly with double distilled (dd) H ... sequence that would have been coded for in the second exon If the band seen in the SIMPL KO tissue lane were the splice variant, then it can be assumed that the folding of this protein sequence could...

Ngày tải lên: 24/08/2014, 12:43

... values, whereas values after months appear to be well over the reference range established by the others Our findings might be of help to explain why TPO values were higher than normal in some ... chosen since some recent evidence has been presented indicating that high levels of this receptor might be found in MPD and might be lowered during anagrelide therapy [14, 30] +40 +20 Platelets ... the platelet count with < 50 % of the baseline value Here, and for the purpose of cytokine levels and platelet counts, we have grouped PR and F together Laboratory methods Blood counts were performed...

Ngày tải lên: 03/11/2012, 10:52

... gradient Within the lung the BALF levels did not reflect the severity of lung injury or the presence of infection per se The BALF levels did both reflect outcome and correlated with the degree of ... write the manuscript GDP helped to recruit patients, analysed the data and helped to write the data WT, NM and MM identified and recruited patients, interpreted the data and helped to write the ... Competing interests The authors declare that they have no competing interests Authors' contributions NN designed the study, recruited patients, performed the analyses, analysed the data and helped to...

Ngày tải lên: 13/08/2014, 10:20

... model and the random-effects model based on the Mantel-Haenszel method and the DerSimonian and Laird method, respectively, to combine values from each of the studies When the effects were assumed ... may encode the GST π isoenzyme in the esophagus, positive effect for esophageal cancer frequently has been detected in those who had some environment exposures such as smoke cigarettes, alcohol ... Ile106Val * Test for heterogeneity Fixed-effects model was used when P value for heterogeneity test > 0.05; otherwise, random-effects model was used Table Association between esophageal cancer...

Ngày tải lên: 25/10/2012, 11:40

... Recherche sur le Cancer and the Agence Nationale pour la Recherche (ANR 05-MRAR´ 033-02) to Gerard Gacon, and by grants to Emmanuel 394 Lemichez from the Agence Nationale pour la Recherche (ANR, ... considered as 100% The data are representative of three independent experiments (B) POSH does not display E3 ligase activity towards Rac1L61 HEK293 cells were transfected with a combination of expression ... ⁄ PAGE minigels and electrotransferred onto nitrocellulose membrane (Schleicher & Schuell, Millipore) Membranes were probed using the indicated primary antibodies and secondary peroxidase-conjugated...

Ngày tải lên: 18/02/2014, 16:20

... spreading on LN and induced cell rounding These changes in cell shape were associated with a decrease in proliferation rate, an increase in cell death, a decrease in progesterone secretion and ... Agricultural Research and Teaching Experimental research was performed with the approval of the regional ethics committee of the Région Centre (Tours, France) During the reproductive season, adult ... pg/tube (60 pg/ well), and the intra- and inter-assay coefficients of variation were less than 10% and 11% respectively Results were expressed as the amount of steroids secreted between 96 h and...

Ngày tải lên: 05/03/2014, 17:20