growth cone · axonal guidance · myelination · extracellular matrix

... documentation of extracellular matrix formed by presence (b, d) or absence (a, c) of transforming growth factor beta (TGFβ) Immunohistochemical documentation of extracellular matrix formed by ... cells, perhaps through contact or growth factor upregulation leading to increased matrix production TGFβ, IGF-1, epider-mal growth factor and platelet-derived growth factor were sig-nificantly ... the transforming growth factor-beta (TGFβ) super fam-ily such as the TGFβ isoforms and the BMPs [15] Also important are the fibroblast growth factor isoforms and insulin-like growth factor (IGF-1)

Ngày tải lên: 09/08/2014, 10:23

... ARTICLE Open Access Differential subcellular and extracellular localisations of proteins required for insulin-like growth factor- and extracellular matrix-induced signalling events in breast cancer ... (Total-AKT1), P-AKT (Ser473), extracellular signal-related kinase-1 and extracellular signal-related kinase-2 (ERK1/2), phosphorylatedextracellular signal-related kinase-1 and extracellular signal-related ... cells, the surrounding microenvironment and the growth factors present in this milieu Cellular attachment and interactions with the extracellular matrix (ECM) regulate biological responses vital

Ngày tải lên: 14/10/2020, 15:05

... MINIREVIEW Viral entry mechanisms: human papillomavirus and a long journey from extracellular matrix to the nucleus Martin Sapp and Malgorzata Bienkowska-Haba Department of Microbiology ... keratinocytes in vitro unless Abbreviations BPV1, bovine PV type 1; CyPB, cyclophilin B; ECM, extracellular matrix; EEA, early endosomal antigen; ER, endoplasmic reticulum; HPV, human papillomavirus; ... the syndecans and glypicans [36,37] In addition, secreted perlecans are abundant in the extracellular matrix (ECM) In vitro studies have shown that infectious entry of HPV33 requires N- as well

Ngày tải lên: 23/03/2014, 04:20

... peripheral zones of growth cones Growth cones, the specialized structures at nerve endings, are known to regulate axonal growth We explored if TRPV1 is localized at growth cones As F11 cells reflect ... endings that extend into growth cone-like structures By co-immunostaining for the growth cone-associated protein 43 (GAP43⁄ neuromod-ulin), a well-characterized growth cone marker [22–25], we ... including growth cones at embryonic stages Overall, our results suggest that TRPV1 regulates the microtubule cytoskeleton and affects growth cone morphology and motility Discussion Growth cones are

Ngày tải lên: 23/03/2014, 09:21

... multisub-unit matrix proteins, which are expressed in cartilage but also inmany other forms of extracellular matrix They participate in theformation of fibrillar or filamentous structures in the extracellularmatrix ... Nanog, however, itsremains unclear, which extracellular matrix and cell adhesion pro-teins are involved in regulation of pluripotency The extracellularmatrix glycophosphoprotein expression of ... (PGs) component of extracellularmatrix Aim of the przesent study was: (i) to compare the expres-sion profile of chondroitin sulphate proteoglycans (CSPGs) ofthe extracellular matrix (ECM), between

Ngày tải lên: 23/03/2014, 15:20

... sulfoxide; ECM: extracellular matrix; Egr: early growth response; ERK: extracellular regulated kinase; IFN: interferon; IL: interleukin; MEK: mitogen-activated kinase kinase; Mmp: matrix metallopro-teinase; ... of the extracellular matrix Type II collagen, aggrecan, and link protein – encoded by the genes Col2a1, Agc1 and Hapln1, respectively – are major compo-nents of the articular cartilage extracellular ... MEK/ERK and Egr1 are required for TNF-regulated catabolic and anabolic genes of the cartilage extracellular matrix, and hence may represent potential targets for drug intervention in osteoarthritis

Ngày tải lên: 09/08/2014, 01:22

... of multiple extracellular signals Keywords: chondrocyte, epidermal growth factor, extracellular matrix, signaling, tumor necrosis factor alpha Introduction The role of epidermal growth factor ... new extracellular matrix molecules In the present study, we investigated the effects of tumor necrosis factor alpha (TNF-α) and epidermal growth factor (EGF) on chondrocyte morphology and matrix ... the lost extracellular matrix contributes to the progression of degenerative disorders of cartilage Inflammatory mediators present in the joint regulate the breakdown of the established matrix

Ngày tải lên: 09/08/2014, 06:22

... and BMP-1 (bone morphogenic protein-1); ECM = extracellular matrix; EGF = epidermal growth factor; IGD = interglobular domain; IL = interleukin; MMP = matrix metalloproteinase; OA = osteoarthritis; ... precursor and extracellular matrix components such as pro-collagen, hyalectans (hyaluronan-binding proteoglycans including aggrecan), decorin, fibromodulin and cartilage oligomeric matrix protein ... forms of Review ADAMTS proteinases: a multi-domain, multi-functional family with roles in extracellular matrix turnover and arthritis Gavin C Jones and Graham P Riley Rheumatology Research Unit,

Ngày tải lên: 09/08/2014, 06:23

... (BMP-2) overexpression on mRNA levels of extracellular matrix molecules and proteoglycan (PG) synthesis overexpression on mRNA levels of extracellular matrix molecules and proteoglycan (PG) synthesis ... matrix turnover in intact and IL-damaged cartilage Moreover, BMP contributes to the intrinsic repair capacity of damaged cartilage Increased matrix turnover might be functional in replacing matrix ... (BMP-2) is one of the candidate growth factors with good potential in cartilage tissue engineering as well as cartilage repair BMP-2 belongs to the transforming growth factor-beta (TGF-β) superfamily,

Ngày tải lên: 09/08/2014, 10:21

... membrane', 'plasma membrane part', 'intrinsic to plasma membrane') and to the extracellular region ('extracellular region', 'extracellular region part') 'Lysosome' and 'endoplasmic reticulum' were ... extracelcel-lular space specific themes ('integral to membrane', 'plasma membrane', 'extracellular region', 'extracellular matrix part') The cellular processes (GO Biological Process) associated with ... analyses highlighted a significant up-regulation of genes and biological themes related to extracellular matrix (ECM) constituents, including members of the integrin family, and suggested that these

Ngày tải lên: 14/08/2014, 08:20

... [37,38] Extracellular matrix-receptor signaling and the focal adhe-sion pathway can be considered part of the same system, as the focal adhesion pathway is activated by extracellular matrix-receptor ... the extracellular matrix-receptor system and 15 components of the focal adhesion signaling pathway It is worth noting that five members of the focal adhesion pathway and the extracel-lular matrix-receptor ... biological function, key pathways have been identified that are associated with inflammation, extracellular matrix interactions and stem cell self-renewal Results Identification of gene products associated

Ngày tải lên: 14/08/2014, 08:21

... MECHANICAL PROPERTIES OF EXTRACELLULAR MATRIX ON CELL BEHAVIORS WONG LONG HUI Trang 2MECHANICAL CHARACTERIZATION OF BIOPOLYMER AND THE EFFECTS OF MECHANICAL PROPERTIES OF EXTRACELLULAR MATRIX ON CELL ... biopolymer networks, which are directly applicable to defining physical parameters in cell and extracellular matrix interactions The first part of the thesis involved the use of conventional macrorheological ... ability to form physiologically relevant 3-dimensional (3D) scaffold to mimic the tissue extracellular matrix (ECM), have made it an attractive option in many biological applications [1,2] The

Ngày tải lên: 09/09/2015, 11:28

... Trang 1BIOLOGY AND EXTRACELLULAR MATRIX BIOCHEMISTRY: BIOPHYSICAL CONSIDERATIONS AND MOLECULAR MODELING HARVE SUBRAMHANYA ... Light Scattering dNTPs, Deoxy-ribose Nucleotide Triphosphates DxS, Dextran Sulfate ECM, Extracellular Matrix EVE, Excluded-Volume Effect Fc70, Ficoll 70kDa Fc400, Ficoll 400kDa FtsZ-GDP, ... presented studies demonstrate the manifold effects of crowding and EVE in molecular biology and extracellular matrix biochemistry with a wide-range of potential applications in academic research, industrial

Ngày tải lên: 11/09/2015, 09:02

... binding of growth factors or growth factor binding proteins The ECM serves as a storage for growth factors, concentrates them, enhances their effect, and even regulates the activity of these growth ... integration, it also serves as a reservoir of growth factors and cytokines Cell attachment to ECM via cell surface receptors such as integrins results in cell-matrix interactions, which regulate cell ... morphology of the ECM cannot be regained 1.3.2 Purified Matrix Components as coating material Various groups have cultured hESCs on single matrix components, such as laminin and Fn Xu et al found

Ngày tải lên: 11/09/2015, 10:06

... hESCs on Matrigel expressed Oct-4, while 62.6% of hESCs on DxSDOC matrix expressed the marker and only 42.2% of hESCs on DxSDOCDOC matrix expressed the marker Oct-4 is a POU transcription factor ... samples, hESCs cultured on DxSDOCDOC matrix showed normal metaphases on all 20 metaphases tested (Figure 5L) 18 of the 20 metaphases of hESCs cultured on DxSDOC matrix showed normal karyotypes, but ... comparisons, the R2 values were found to be more than 0.95, therefore hESCs under all the three matrix conditions were similar to each other, with only slight differences Interestingly, the R2

Ngày tải lên: 11/09/2015, 10:07

... Engel J Domain structure and organisation in extracellular matrix proteins Matrix Biol 21(2):115-128 (2002) 63 Schönherr E, Hausser HJ Extracellular matrix and cytokines: a functional unit Develop ... transforming growth factor-β binding protein in storage of latent TGF-β in the extracellular matrix and as a structural matrix protein J Cell Biol 131(2):539-549 (1995) 31 Annes JP, Munger JS, Rifkin ... components of extracellular matrix microfibrils J Histochem Cytochem 44(8):875889 (1996) 30 Dallas SL, Miyazono K, Skerry TM, Mundy GR, Bonewald LF Dual role for the latent transforming growth factor-β

Ngày tải lên: 11/09/2015, 10:07

... destination where axonal growth cone survival is no longer completely inhibited (Marquardt et al., 2005) Although Eph-ephrin activation is usually associated with decreased growth cone survival and ... Axon Guidance Molecules Growing axons rely on a variety of guidance molecules in deciding upon a growth pathway Biochemical and genetic studies have revealed a variety of families of axon guidance ... kDa) extracellular matrix proteins containing leucine-rich repeats and epidermal growth factor-like repeats (Fig 3) Slit is a secreted protein which is most widely known as a repulsive axon guidance

Ngày tải lên: 19/11/2015, 15:47

... and prognostic impact of circulating markers of inflammation, endothelial activation and extracellular matrix remodelling in patients with lung cancer and chronic obstructive pulmonary disease ... Methods: Serum levels of 18 markers reflecting inflammation, endothelial activation and extracellular matrix remodelling were analysed in 207 patients with non-small lung carcinoma (NSCLC) before ... COPD status Conclusion: Several markers reflecting inflammation, endothelial activation and extracellular matrix remodelling are elevated in serum from patients with COPD compared to LC patients

Ngày tải lên: 03/07/2020, 00:50

... cultured on PuraMatrix™ or Growth Factor Reduced (GRF) Matrigel™ Collective IC50 results of doxorubicin activity on MDA-MB-231 (c) and MCF-7 (d) cells cultured in the presence of PuraMatrix or GFR ... Matrigel™ enabling cellular arrangements into a 3D architecture in conjunction with cell-to-extracellular matrix (ECM) contact Results: Breast cancer cells cultured in a 3D ECM-based model demonstrated ... mechanisms which may contribute to reduced doxorubicin sensitivity Keywords: Doxorubicin, Extracellular matrix, Three-dimensional cell culture, Drug resistance Background Breast cancer has the

Ngày tải lên: 24/07/2020, 00:07

... L E Open AccessGene and miRNA expression signature of Lewis lung carcinoma LLC1 cells in extracellular matrix enriched microenvironment Vaidotas Stankevicius1,2, Gintautas Vasauskas1,2, Danute ... Jarmalaite1,4, Ricardas Rotomskis1,5and Kestutis Suziedelis1,2,6* Abstract Background: The extracellular matrix (ECM), one of the key components of tumor microenvironment, has a tremendous impact ... genes and 77 miRNAs were significantly altered in LLC1 cells after 48 h cell growth under 2D and ECM based 3D cell growth conditions Pathway enrichment results indicated metabolic pathway, MAP

Ngày tải lên: 20/09/2020, 18:15