genomics discovery and functional analysis of genes

... ClMPK and 2 ClMKK genes had available EST sup-ports (Table 1), representing 93.3 and 33.3 % of the ClMPK and ClMKK genes, respectively We attempted to clone the full-length cDNAs of all ClMPKs andClMKKsfor ... 65 % of identity at amino acid sequence leveland are also not tandem duplicated genes It is thuslikely that tandem duplication plays a limited role in theevolution of the ClMPK and ClMKK genes ... patterns and functional analysis of the MAPK and MAPKK genes in watermelon (Citrullus lanatus) Qiuming Song1, Dayong Li1, Yi Dai1, Shixia Liu1, Lei Huang1, Yongbo Hong1, Huijuan Zhang1,2* and Fengming

Ngày tải lên: 26/05/2020, 20:34

... class A and E genes determine the sepals; class A, B, and E genes specify the petals; class B, C, and E genes regulate the stamens fate; class C and E genes control the carpel for-mation; and class ... that these genes were highly expressed in floral organs Fig 3 Expression levels of TeAG and TeAGL11 genes in different tissues and organs of marigold (a) Heatmap of relative expression of TeAG1, ... the downregulation of STK Expression profile analysis of endogenous genes related to early flowering and curled leaves We also detected the expression level of endogenous genes related to flowering

Ngày tải lên: 28/06/2021, 20:55

... ClMPK and ClMKK genes had available EST supports (Table 1), representing 93.3 and 33.3 % of the ClMPK and ClMKK genes, respectively We attempted to clone the full-length cDNAs of all ClMPKs and ... expression-based functional analysis in Nicotiana benthamiana Our characterization of the watermelon ClMPK and ClMKK families provides a useful platform for further functional studies of ClMPKs and ClMKKs ... AtMPKs and AtMKKs as queries and identified 15 and non-redundant sequences that are putative MPK and MKK genes, respectively The predicted amino acid sequences of the putative ClMPKs and ClMKKs

Ngày tải lên: 02/11/2022, 08:52

... extraction from E coli and HPLC analysis For the functional analysis of EgcrtE, E coli strain BL21(DE3) was transformed with both pET-EgcrtE and pAC-PacrtB For the functional analysis of EgcrtB, E coli ... E coli cells for the functional analysis of EgcrtE and EgcrtB with HPLC in cooperation with SK TS conceived of the study, and participated in its design and coordination; and helped to draft the ... from a group of GGPP synthase and phytoene synthase proteins, respectively, of algae and higher plants In addition, we investigated the effects of light stress on the expression of crtE and crtB

Ngày tải lên: 26/05/2020, 20:40

... reports a genome-wide annotation of the WRKY family in soybean and a functional analysis of some genes involved in response to P pachyrhizi infection Results Annotation and in silico characterization ... groups and subgroups in accordance with Eugelm et al [24] Group I, II and III contained 31, 126 and 25 soybean WRKY genes, respectively (Table and Additional file 1) A total of 13, 33, 42, 16 and ... selection of pathogen resistance and, in severe cases, are ineffective [16] In this context, understanding the molecular basis of the soybean defense against fungal infection and growth, identifying genes

Ngày tải lên: 27/05/2020, 01:13

... N-terminus of HALT-1 resulted in a change of the native structure and thus the loss of protein function Trang 9Strands β6, β7 and helix α2 of actinoporins contain a conserved cluster of aromatic ... polar head group of the cell membrane, and light blue highlights the only cysteine present in HALT-1 The α-helix and β-strand of HALT-1 are predicted by PSIPRED and marked on the top of the sequence ... Mutagenesis and Functional Analysis of the Pore-Forming Toxin HALT-1 from Hydra magnipapillata Yvonne Jing Mei Liew, Wai Tuck Soh, William Febry Jiemy and Jung Shan Hwang * Faculty of Applied

Ngày tải lên: 02/11/2022, 14:24

... HPLC analysis of diazepam of the spheroid model) and compared them to the same conditions in the sandwich model in order to discover which are more suitable Results and Discussion 2.1 Role of FCS ... Monoculture and Co-Culture in the Same Culture Conditions in Both the Spheroid and Sandwich Models LDH activity of the cell culture of 10 million cells in the spheroid model and sandwich model ... cytoplasm and nucleus (Figure 4(iii)A, B and C) Biomolecules 2013, 249 Figure Immunological detection of different liver cells in spheroid culture model (i) A and B: localization of Ito cells (A) and

Ngày tải lên: 02/11/2022, 14:35

... QUANTIFICATION OF THE FUNCTIONAL GENES aprA AND dsrA OF SRP AND 16S rRNA OF TOTAL BACTERIA IN MARINE SEDIMENT SAMPLES Depth profiles of DNA copy numbers of the functional genes aprA and dsrA as marker ... quantification and diversity analysis of thefunctional genes aprA and dsrA of sulfate-reducing prokaryotes in marine sediments of the Peru continental margin and the Black Sea Anna Blazejak 1 † and Axel ... 106–107copies/g between 0.18 and FIGURE 1 | Depth profiles of DNA copy numbers of the functional genes aprA and dsrA as marker for sulfate-reducing prokaryotes (SRP) and the 16S rRNA gene of total Bacteria

Ngày tải lên: 04/12/2022, 16:12

... been applied in both model and non-model organisms to discover and document the role of specific genes in adaptive evolution [2-6] Surveys of hundreds to thousands of genome-wide markers identified ... number of loci that can be examined (usually hundreds) and the focus on anonymous loci limits identification of functionally relevant genes [22] Genome-wide scans of genetic diversity at tens of ... K Manier1,2 and Stephen R Palumbi1 presented typing of Site loci and the quantitative is of polymorphic Tiling Analysis A method thousands of loci in individualsgenoRestrictionfor the simultaneous

Ngày tải lên: 09/08/2014, 20:21

... biosynthesis/degradation genes were defined, and a particularly high level of haplotype variation was uncovered in the genes encoding sucrose synthase (types I and II) and starch synthase The ability of a panel of ... parents of mapping populations cvs Steptoe and Morex [43], the parents of the Oregon Wolfe population [44] and the parents of AB popula-tions cv Scarlett and ISR42_8 [22], and cv Brenda and the ... overrepresented in groups 1 and 2 included tran-scription regulators, genes induced by abiotic stress, genes responsible for the synthesis of storage proteins and genes related to amino acid and carbohydrate

Ngày tải lên: 11/08/2014, 11:21

... factors Expression analysis of BnWRKY genes in response to fungal pathogens-S sclerotiorum and A brassicae Because the divergence of paralogous genes is often after the sub-functionalization [84], ... Because of the economic importance of this crop, and its evolutionary relationship to Arabidopsis thaliana, we sought to characterize a subset of canola WRKY genes in the context of pathogen and ... Identification and expression analysis of WRKY transcription factor genes in canola (Brassica napus L.) in response to fungal pathogens and hormone treatments and Nat NV Kav*1 Address: 1 Department of Agricultural,

Ngày tải lên: 12/08/2014, 03:20

... expression and functional analysis of HD-Zip III genes in angiosperm and gymnosperm forest trees BMC Plant Biology 2010 10:273 Submit your next manuscript to BioMed Central and take full advantage of: ... consolidate the list of candidate targets Future studies could continue to explore and compare more broadly the role of HD-Zip III genes in primary and secondary vascular growth of woody plants, ... Hasebe M: Isolation of homeodomainleucine zipper genes from the moss Physcomitrella patens and the evolution of homeodomain-leucine zipper genes in land plants Molecular Biology and Evolution 2001,...

Ngày tải lên: 11/08/2014, 11:21

... Here we confirm and extend earlier studies of these AAA-peroxins and give a further detailed functional analysis of their cassette structure and interaction The interaction of Pex1p and Pex6p involves ... biological function of Pex1p and Pex6p 51 Domain function of Pex1p and Pex6p I Birschmann et al A B C D E F Fig Effects of point mutation of the WalkerA and B motifs of the AAA-cassettes of Pex1p on ... (D2) of Pex1p and Pex6p are essential for peroxisomal biogenesis To investigate the effects of the described WalkerA and WalkerB mutants of the ATP-binding sites for the function of Pex1p and...

Ngày tải lên: 07/03/2014, 16:20

... (featuring a Km of 3.1 lM and an apparent Vmax of 28.49 lmolÆh)1Æmg)1) and a substrate inhibition curve (featuring a Km of 13.5 lM, an apparent Vmax of 89.47 lmolÆh)1Æmg)1 and a Ksi of 5.65 lM) were ... 0.25 lm) and programmed at an initial temperature of 45 °C for min, with a ramp of 10 °CÆmin)1 to 280 °C, and final time of 10 Products were identified by comparison of retention times and mass ... by random sequencing of a flavedo-derived cDNA library of C limon and their characterization by functional expression in Escherichia coli MATERIALS AND METHODS Plant material, substrate, and reagents...

Ngày tải lên: 08/03/2014, 23:20

... 0.667 [30]) A value of 1.36 was taken for the refractive index n [31] MALDI-TOF analysis MALDI-TOF analysis of the peptides obtained after tryptic digestion of the labeled and nonlabeled Ag-NPA-1 ... incorporation of the ligand Binding of DAUDA to Ag-NPA-1 caused  20% quenching of the emission of Trp17 indicating FRET between the single Trp residue and the dansyl group We calculated a value of 3.74 ... Trp chromophore of ABA-1, the single Trp of Ag-NPA-1 is a sensitive marker of the protein conformation and its emission reflects conformational changes after ligand binding Analysis of FRET from...

Ngày tải lên: 16/03/2014, 18:20

... FEBS 1867 Analysis of upstream region of the Pokemon gene Y Yang et al decoy experiments using lg of the NEG-U decoy, lg of the NEG-D decoy, and a combination of lg each of the NEG-U and NEG-D ... different constructs in A549 and DU145 cells (C) The effects of lg of the NEG-U decoy, lg of the NEG-D decoy and a combination of lg of each decoy on the activity of F-560 The open ellipse indicates ... indicated above each lane (B) Activities of F-233 and MF-233 in A549 and DU145 cells (C) Activities of F-233 and varying amounts of the decoy oligonucleotides in A549 and DU145 cells WP-decoy indicates...

Ngày tải lên: 30/03/2014, 04:20

... residues of D3 and B contribute to the stability of the dimer The first cluster includes F70/V336 and I72/Q338 (both in b5 strand) of D3 and F27/Y289 (b2 strand), L67/M324, V70/I327 and L72/L328 ... b5 strand) of D3 and I41/L304, C43/A306 (both in b3), L69/S326 and L71/L328 (both in b4) of B Stacking interactions between guanidinium groups of arginines R69/V335 of D3 and R25/G287 and R49/T312 ... in b4 strand) of B The second cluster consists of P6/M267, L10/ L271 (both in a-helix), V18/C279 (b1 strand), L32/F293 (b2 strand), I33/K294 (loop after b2 strand), I68/F334, L71/ V337 and L73/F339...

Ngày tải lên: 30/03/2014, 15:20

... Determination of the antigenic phenotype and frequency of subsets of WC1+ and WC1- γδ T cells in peripheral blood and lymphoid tissues: i) Flow cytometric analysis: Analysis of the tissue distribution of ... expression of WC1N3 and WC1-N4 isoforms and expression of families of the γδ TCR that express determinants TCR1-N6, TCR1N7, or TCR1-N6 and -N7 Only a subset of WC1- γδ T cells expressed a form of TCR1 ... pattern of expression of TCR1N6+ and TCR1-N7+ cells also suggested expansion of the WC1+ population of γδ T cells, in the course of evolution, included selective usage of a subset of TCR1 Vγ, Jγ, and...

Ngày tải lên: 07/08/2014, 14:22

... Structural and functional analysis of hUpf1……………………….41 1.7.1 Previous functional and biochemical studies of Upf1……………… .41 1.7.2 Structural studies of Superfamily helicases……………………………43 1.7.3 Aims of ... STRUCTURAL AND FUNCTIONAL ANALYSIS OF CRITICAL PROTEINS INVOLVED IN mRNA DECAY CHENG ZHIHONG (B.Sc) Ease China University of Science and Technology A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY ... assay of Dhh1………………………………… 109 Figure 4-7 In vivo mutagenesis analysis of Dhh1……………………………… 113 Figure 4-8 Analysis of limited trypsin digestion of Dhh1………………………….115 Figure 4-9 Comparison of Dhh1...

Ngày tải lên: 14/09/2015, 22:22

... exist in exon Restriction analysis was performed using 10μl of PCR product, 7μl of dH2O, 2μl of NEB Buffer (10X) and 1μl (10 units/μl) of BstUI (New England BioLabs) and incubated at 600C for ... with 50μl of DNase incubation mix (40μl of yellow core buffer, 5μl of 0.09M MnCl2 and 5μl of DNase I enzyme) and incubated at room temperature (20-25°C) for 15 minutes Immediately 200μl of SV DNase ... creates a unique restriction site and it produces two bands (600bp and 200bp) Three bands (800bp, 600bp and 200bp) will be found in case of heterozygotes 2.4.3 Sequencing of PCR product After detecting...

Ngày tải lên: 15/09/2015, 17:10