genomic structure and genes of hhv 8

... copy of the ORF50 and A6 in the TR Importantly, removal of C500-DT from the LUR not only resulted in the absence from this region of ORF50 and A6, but also of the first 163 bp of ORF48 and the ... Virus research 188, 68–80 (2014). 28 Cha, T A et al Human cytomegalovirus clinical isolates carry at least 19 genes not found in laboratory strains Journal of virology 70, 78–83 (1996). 29 Maotani, ... almost identical to that of the parental strain, and we discovered and localised a duplicated and translocated region encoding ORF50 and A6 as well as partial sequences of ORF48 and A7 Since this duplication

Ngày tải lên: 04/12/2022, 10:35

... Central/South England, 50 randomly chosen from each of Devon and Cornwall, and 200 randomly chosen from the north of England This final combined dataset had a total coverage of 431,048 common SNPs ... grouped Figure 1 Clustering of 34 Irish Travellers, 300 Settled Irish, and 828 British by fineStructure (A) The first and second components of principal component analysis of the haplotype-based ... excess of ROH and IBD segments The ROH profile of the Irish Travellers is comparable to other consanguineous populations such as the Balochi of Pakistan and Druze of the Levant However, of the

Ngày tải lên: 04/12/2022, 10:35

... processes overlap and are closelylinked so that the form and structure of the plant evolves as the integration of many consecutive and interactingprocesses THE STRUCTURE AND DEVELOPMENT OF THE CEREAL ... diagram of a mature wheat plant highlighting tiller, leaf and internode numbering and position (redrawn from Kirby and Appleyard, 1987). Figure 2.3 Detailed structure of the stem and leaf of the ... formation of florets within each spikelet and, later, the regression and death of some florets and spikelets and the death of some tillers This phase is also the time of greatest dry mass increase and

Ngày tải lên: 08/03/2014, 23:20

... comprising mainly the b-strands. The loops between strands B and C, known as the ‘hypervariable loop‘, D and E, E and F, F and G, and G and H are particularly tolerant of longer insertions [19]. ... collected at 300 K of single CCP1 and CCP2 and of pair of modules at neutral and acidic pH Doc S1 Intermodule cooperativity in the structure and dynamics of consecutive. ... Set of HSQC spectra of CCP1CCP2 (#2) collected at 280, 300 and 320 K at pH 4.0 Fig S11 Set of HSQC spectra of CCP1CCP2 (#2) collected at 300 and 315 K at pH7.0 Fig S12 Overlay of

Ngày tải lên: 15/03/2014, 23:20

... minimized structures and the mean structure in SDS at pH 6.0 were 0.18 ± 0.06 and 0.85 ± 0.17 A˚ for the backbone and all heavy atoms, respectively, in the segment Leu9–Phe18, vs 0.20 ± 0.06 and 0.89 ... scan rate of 50 nmÆmin)1with a respond time of 0.25 s, step resolution of 0.1 nm and band width of 1 nm Each spectrum was obtained from the average of four scans Prior to calculation of final ellipiticity, ... range of well-defined residues, i.e Leu9–Phe18 in SDS (pH 6.0) and Leu9–Phe20 in TFE, 99.4% and 91.7% occupy the most favored regions of the Ramachandran space in SDS and TFE, respectively, and

Ngày tải lên: 16/03/2014, 16:20

... b-strands are numbered and the b5 and b7 strands are shown in red and blue, respectively G104 (equivalent to K137 of human HSP22) and R108 (equivalent to K141 of human HSP22) are shown in purple and ... importance of this part of molecule of the sHsp is supported by the fact that mutations in the loop connecting the b5 and b7 strands, or in the b7 strand of sHsp, often correlate with the development of ... the structures of M jannaschii Hsp16.5 and T aestivum Hsp16.9 with the corresponding structures of three human sHsp [30] The elements of the secondary structure of M janna-schii Hsp16.5 and

Ngày tải lên: 23/03/2014, 07:20

... concentra- tions of 15.54 and 35.8 mm of Pb?*, while far CD studies show considerable change in the secondary structure of Blg-B by increasing the concentration of Pb** Near CD and fluorescence ... conformation of F-actin at various stages of filament growth during polymerization of CaATP-, MgATP-, and MgADP-G-actin Effects of the type of polymerizing salt, filament stabilization by phalloidin, and ... EcoR124I E Sisakova and M Weiserova Laboratory of Molecular Genetics of Bacteria, Division of Cell and Molecular Microbiology, Institute of Microbiology, Czech Academy of Sciences, Prague,

Ngày tải lên: 23/03/2014, 15:20

... 600 MHz and temperatures of 288 K and 298 K A reference [1H,15N]-HSQC spectrum was recorded with fully protonated Xis_C28S The sample was then lyophilized and dissolved in the same volume of ice-cold2H2O ... of Phe31 and the carbonyl oxygen of Val48 The experimentally determined Fig 5 Solution state structure of HK022 Xis_C28S (A)Stereoview of the 20 conformers representing the final HK022 Xis_C28S ... consists of five b-strands, whereas k Xis features only two regular b-strands and two extended sequence segments [20] Second, the
wing b-sheet of HK022 Xis_C28S consists of three b-strands and is

Ngày tải lên: 23/03/2014, 15:21

... lysozyme [12,13] The native fold of a-lactalbumin is stabilized by the high-affinity calcium-binding site, coordinated by the side chains of asparagines 82, 84, 87 and 88 and lysine 79 [14] The helical ... 23–34 and 86–98) and two short 310-helical domains The smaller b-sheet domain consists of a triple-stranded antiparallel b-sheet (amino acids 40–50) One disulfide bond connects a-helical and b-sheet ... the form and function of novel complexes such as HAMLET and ELOA, we have provided new evidence that a loss of native structure can endow proteins and their complexes with distinct and beneficial

Ngày tải lên: 29/03/2014, 21:20

... surface potential of AFP (left) and PAF (right) structures representing the orientation of the side chain lysine resi- dues. Lys9, Lys35 and Lys38 of PAF and Lys9, Lys32 and Arg35 of AFP are the ... indicate b-strands. G. Batta et al. Structure and dynamics of an antifungal protein FEBS Journal 276 (2009) 2875–2890 ª 2009 The Authors Journal compilation ª 2009 FEBS 2877 b-strand secondary structure. ... 2009 FEBS 2881 The second half of loop 3 is one of the most highly- conserved regions of PAF: three lysine side chains (Lys34, Lys35 and Lys38) from this loop give rise to a high density of positive

Ngày tải lên: 29/03/2014, 23:20

... citrate, competitive 0.92 and 518 versus noncompetitive 0.95 and 351; isocitrate, competitive 0.98 and 121 versus noncompetitive 0.99 and 58. Trang 6Fig 4 Tetrameric structure of 3-carboxy-cis,cis-muconate ... (Arg155 and Arg312) are indicated by dashed lines The side chain of His278 is not visible in the ArCMLE1 structure The figure was created from the coordinates of the P212121 structure of ArCMLE1 and ... Finally, ArCMLE1 Gln308A is replaced by His308A, and Val289D and Thr290D on helix 283–308 are both mutated to Ala in AtCMLE1 These changes might affect the flexibility at the back of the active site

Ngày tải lên: 30/03/2014, 10:20

... Asp99 and Phe102, Met104 and Ile107, and Ser108 and Glu111, which marks the start of aC The entire flexible loop packs between the N-terminal portion of aC and part of the C-lobe (Figs 2A and 3A) ... the structure of APO-Rio1 presented here, this loop con-sists of 27 amino acids (Arg83 of b3 through Glu111 of aC) and is significantly longer than the 18 amino acids long disordered loop of Rio2 ... the structure of Rio2 bound to ATP and Mn2+, the c-phosphate is buried through the ordering and binding of three residues of the N-terminal end of the flexible loop [21] The P-loops of Rio1 and

Ngày tải lên: 30/03/2014, 20:20

... lower energies because of the increase of the Si-Si resonance peak at 1842 eV and reduction of the peak related to Si-N bonding located at 1845.5 eV The weak Si-O peak at 1848 eV in the crystalline ... effects of the anneal-ing time on the growth, structure and luminescence of SRSN films are addressed in ‘Isothermal anneals at 600° C’ and ‘Isothermal anneals at 800°C’ below The electronic structure ... of the film and should not be taken as an indication of Si-O bonding within the bulk of these films Figure 4 compares the silicon L3,2 -edge spectra for PECVD and ICP CVD AD films Both sets of

Ngày tải lên: 21/06/2014, 05:20

... result of changing genera-tions of beech In Krkonoše Mts the largest reported tree is silver fi r with 182 cm of dbh and 58 m of height Total volume of forest stands varies between 500 and 900 m3·ha–1 ... selected stands with the absence of silver fi r (PRP 6 and 28) virtual underplanting of this species was done and prediction of development after 25 and 50 years performed Th e forest stand diversity ... result of destruction of the ageing stand Table 2 presents growth variables after spontaneous development for the whole stand and beech as dominant tree species Mixed stands Mixed forest stands of

Ngày tải lên: 07/08/2014, 10:21

... 1988; Lähde et al, 1991, 1992). Bøhmer, 1957; Mitscherlich, 1963; Kern, 1966; Hasse and Ek, 1981; Pretzsch, 1985; Solomon et al, 1986; Haight and Gets, 1987; Lundqvist, 1989), the comparison of ... mixed stands of birch and spruce grew better than pure spruce stands (Otto, 1986). structurally different stands The trees in in conifer stands (Frivold, 1982; Mielikäi-nen, 1985) Mixed stands ... and for the same average volume class, and as a relative growth (%) The effect of stand structure and tree species composition on different stand parameters was analysed with 2-way analysis of

Ngày tải lên: 08/08/2014, 19:21

... The survival of Scots pine as an under-storey is also good on virgin and drained peatlands (Heikurainen, 1971; Hånell, 1984; Gustavsen and Päivänen, 1986; Hökkä and Trang 7Laine, 1988; Hökkä et ... the case of 31% of sample plots while 17% were irregularly uneven-sized in structure The number of trees per hectare in the all-sized stands was more than twice that of even-sized stands The ... (Reynolds, 1969) and Ponderosa pine (Pinus ponderosa Dougl ex Laws) (Roe, 1952; Alexander and Edminster, 1978) can be raised as all-sized stands According to Foiles (1978), such stands must be logged

Ngày tải lên: 08/08/2014, 19:21

... ª 20 08 The Authors Journal compilation ª 20 08 FEBS T N Rampias et al Genomic structure and expression analysis of the Cc RNase epidermis and intestine of 6-day-old larvae and adult heads and ovaries, ... molecule of 1.5 kb, referred to as Cc RNase cDNA 02 (EMBL database accession number AJ874 689 ) This novel cDNA clone has a precise length of 1466 bp and contains a 5¢ UTR of 1 38 bp, an ORF of 288 nucleotides ... (rectangles) and intron (horizontal lines) structure of the ORF region of the RNase j genes from Homo sapiens (NC_000017: 685 6522– 685 8575), Mus musculus (NC_000077:c700533 48 700516 28) , Rattus norvegicus...

Ngày tải lên: 23/03/2014, 06:20

... HT22Hch HT374Ta HT 382 Ta HT 382 Hch 0,25 0,10 0,05 0,15 0,10 0,05 0,00 0,00 -0,05 76 78 80 82 84 86 88 -0,05 90 76 78 80 82 Temperature ºC H1 H106 84 86 88 90 Temperature ºC T22 HT22 BW2 08 Ta 200bp 100bp ... Chinese Spring and a 5Hch addition line of cv Chinese spring), and tritordeum (HT374, HT 382 and HT22) wild (H11 and H2 08) and cultivated barley (H106) and is identical in H11 and H2 08 Consequently, ... 440 48. 424 82 .1 92 91.5 n/a n/a n/a n/a 1323 64.2 440 48. 081 7.31 n/a 91.9 89 .8 3244 3240 52.2 52.3 9 10 10 1323 1323 64.3 64.6 440 440 48. 315 48. 273 6.94 6.97 82 .9 83 92.5 92.6 91.3 91 .8 (1)...

Ngày tải lên: 12/08/2014, 03:20

... heterochromatin 2204 81 82 83 84 85 86 87 88 89 90 91 92 organization, chromosome segregation, and mitotic progression Mol Cell Biol 20, 37 28 3741 Gause M, Schaaf CA & Dorsett D (20 08) Cohesin and CTCF: ... Chem 261, 383 8– 384 5 95 Garrard WT (1991) Histone H1 and the conformation of transcriptionally active chromatin Bioessays 13, 87 – 88 96 Kamakaka RT & Thomas JO (1990) Chromatin structure of transcriptionally ... Cell 84 , 84 3 85 1 Hirschhorn JN, Brown SA, Clark CD & Winston F (1992) Evidence that SNF2 ⁄ SWI2 and SNF5 activate transcription in yeast by altering chromatin structure Genes Dev 6, 2 288 –22 98 Kwon...

Ngày tải lên: 14/02/2014, 18:20

... domains of Spir-2 and PTPN13 were generated by RT-PCR and inserted into pEGFP-C1 and pGEX-4T-2 The N- and C-terminal regions of the v-KIND KIND2 and the MAP2 CD were generated by PCR and cloned ... downstream of v-KIND overexpression [12]) enhances the activity of MAP2 to bind to microtubules and promote their assembly [ 18] , and that the inhibition of JNK1 (such as downstream of v-KIND knockdown ... series of GST-fused deletions of the CD2-1 region (Fig 3E) and examined the interactions between bacterially expressed proteins of these subregions (Fig S1C) and 1654 Fig Of all members of the...

Ngày tải lên: 14/02/2014, 19:20