genetics gene expression and phenotype

... a, c, e, g, I and k) and meniscectomized (b, d, f, h, j and l) joints are shown Scale bar: 250 µ m. Trang 7In the present study the changes observed in the expression of aggrecan and type II collagen ... University for her assistance with the animal handling and care. References 1. Maroudas AI: Balance between swelling pressure and collagen tension in normal and degenerate cartilage Nature 1976, 260:808-809. ... III, and VI sequester and modulate the multifunctional cytokine oncostatin M J Biol Chem 2002, 277:3242-3246. 43 Aigner T, Zien A, Gehrsitz A, Gebhard PM, McKenna L: Anabolic and catabolic gene expression

Ngày tải lên: 09/08/2014, 06:23

... article Plant origin and ploidy influence gene expression and life cycle characteristics in an invasive weed Müller-Schärer4 and Jorge M Vivanco*1,2 Fribourg, Switzerland Email: Amanda K Broz - akbroz@lamar.colostate.edu; ... actin, ubiquitin, and cytochrome c oxidase were also analyzed as controls to nor-malize the expression of candidate genes (Table 3) Gene expression analysis RNA extraction and cDNA synthesis ... appropriate standard curve for each gene of interest and normalized using the geometric mean of the three standards: actin, cytochrome c oxidase (COX) and ubiquitin (UBQ), as suggested in Vandersompele

Ngày tải lên: 12/08/2014, 03:20

... cloned and cultured as PBMCs from HAM/ TSP, and all generated clones were assayed for HTLV-1 infection, tax expression, CD4+ and CD8+ expression, cell cycling and apoptosis, as shown in Figure 1 and ... cell/ well and cultured during 1.5 – 3 months in the same conditions Then cells were assayed for HTLV-1 infection and integration, tax expression, CD4+and CD8+expression, cell cycling and apoptosis, ... without malignancy also add up nuclear and mitotic defects typical of genetic instability related to theexpression of the virus-encoded oncogene tax HTLV-1 expression is cancer-prone in vitro,

Ngày tải lên: 12/08/2014, 23:23

... regions of histone modification and active promoters [11,12] Until now the study of global gene expression in T gondii and the use of expression data to inform gene annotation has been almost ... trans-lational control [7-9] and the potential regulation of ribosome expression has also been proposed [10] Evidence may also point to possible epigenetic control of gene expression, fol-lowing observations ... gels and assuming post-translational variants are the products of a single gene, these data repre-sent the expression of 616 non-redundant Toxoplasma genes, of which 547 correspond to release4 gene

Ngày tải lên: 14/08/2014, 20:22

... volume and gene expression Further, the geometry sensitive histone Trang 12deacetylases and transcription factors and co-factors which alters chromatin compaction and modulate early gene expressions ... geometrical cues and how it correlates with chromatin compaction and gene expression The critical molecular intermediates involved in the geometry regulated chromatin compaction and gene expression ... (1800µm2) and triangle and circle of equal area (1800µm2) respectively are shown in Fig 2.5A, B and C Gene expression profiles between cells of varying sizes revealed actin and actin related genes

Ngày tải lên: 10/09/2015, 09:04

... Cold-induced BAT Gene Expression VLCFAs and the ELOVL Gene Family The ELOVL3 Gene Time course of ELOVL3 Cold-induced Expression The ELOVL3 Knock-Out (KO) Model Elovl3-ablation and Lipid Recruitment ... Lipogenesis in Liver and Adipose Tissue Brown Fat mRNA Extraction cDNA synthesis and qRT-PCR Gene Expression Analysis Trang 6Results Experiment 1: Effect of Temperature on Brown Fat Gene Expression ... mammals Group Housing and Behavioral Thermoregulation Exercise and Thermoregulation Homeothermic Thermogenesis Shivering Thermogenesis Brown Adipose and Non-shivering Thermogenesis White vs Brown

Ngày tải lên: 21/09/2015, 21:25

... duplication and that silicatein β gene share traits with both cathepsin L and silicatein Conservation of gene structure and exon length in silicatein and cathepsin L genes suggests Trang 3that these genes ... spicule number and silicatein gene expression throughout A queenslandica development……… 59 List of Tables Table 2.1 Intron phases for A queenslandica silicatein and cathepsin L genes, and S ... better understanding of how spiculogenesis is genetically regulated By using A queenslandica I can trace the expression and role of silicatein genes through embryonic, larval and postlarval

Ngày tải lên: 13/10/2015, 14:07

... canine origin and determined their genetic fingerprint through PCR and microsattelite analyses, creating a point of reference for validation in future studies and collaborations Gene expression ... available genomic and drug sensitivity data we have generated, and to show its utility in comparative and translational oncology by focusing specifically on canine melanoma and osteosarcoma We ... LINE (FACC) PANEL IN COMPARATIVE AND TRANSLATIONAL STUDIES OF GENE EXPRESSION AND DRUG SENSITIVITY Submitted by Jared S Fowles Graduate Degree Program in Cell and Molecular Biology In partial

Ngày tải lên: 07/08/2019, 02:33

... deformities at hatch, larval survival and expression of targeted genes relating to larval development (gh and igf) and stress (hsp). Here, the expression of targeted genes was affected by larval age/stage and temperature, as well as their interaction when ... be close to the thermal regime of the recently identified natural spawning area of this species [16] Gene expression In the present study, we observed an increased expression of hsp70 and hsp90 with increasing age and stage, while expression levels increased towards both, colder (16°C) and warmer (22°C) thermal limits. The expression of hsp has previously been linked to ... (similarly to hsp70) lower than for the other temperatures throughout development. Larval age influenced gene expression of hsp90 when larvae were reared in 16, 18, 20 and 22°C (P < 0.010; Fig 4K–4N) and relationships between developmental age and hsp90 expression were best explained by quadratic parabola regressions at 16°C (y = 1.35–0.32 x + 0.02 x

Ngày tải lên: 26/09/2019, 16:15

... RT-PCR, slide and tube coagulase test Additionally, YB downregulated seb, sec, sed, and tsst-1 mRNA expression to suppress enterotoxin and tsst-1 secretion and adhesion function related genes sarA, ... enterotoxin and tsst-1 expression using RT-PCR Figure 7 The role of YB on adhesion function related genes expression of S aureus YB inhibits adhesion function related genes sarA (A), icaA (B), and cidA ... function related genes sarA, icaA, and cidA mRNA expression Taken together, the data suggest that YB may reduce HAPU via suppressing virulence gene expression and biofilm formation of S aureus

Ngày tải lên: 15/01/2020, 10:47

... excessive ROS, and the expression of alternative oxi-dase gene (AOX1a) is also known to respond to various stresses in plants [58] The expression ofAOX1 affects both ROS and RNS generation and accumulation ... expressed genes (DEGs) among treatments Analysis by GenSpring indicated that a total of 965, 1662 and 3783 genes showed significantly up-regulated expression and a total of 287, 1511 and 3261 genes ... GO:0004601 exist in a big gene family, and many ofVvPOD genes were up-/down-regulated, especially by HC and PHC (10/14, and 15/10) Obviously, most of thePOD genes were upreg-ulated and they are assumed

Ngày tải lên: 22/05/2020, 04:44

... the turtle RfBP gene and its effects on flowering and expression of FD and AP1 genes in Arabidopsis WT, RfBP + , and RfBP−plants were grown in long days Northern blotting (a) and electrophoresis ... of gene expression levels between RfBP + and WT plants, and locate RfBP-affected genes with colored square patterns and other genes with grey dots in METC Electron-carrier protein com-plexes and ... categories By contrast, the expression of FT and photoperiod genes in leaves and the expression of FD and AP1 in the shoot apex were no longer enhanced when the RfBP gene was silenced, RfBP protein

Ngày tải lên: 26/05/2020, 23:27

... of chemokines and their receptors [10–12] Cytokines and chemokines in pSS In patients with pSS the salivary and lacrimal glands are the main target organs for the immune system and, at least ... encoded by VκA2, O8/O18, L11, A17 and A27 [62] Moreover, Vλ genes of the Vλ2 and Vλ7 family were found in the Hib-antibody VL gene repertoire [62] In addition, VκA27 and Vλ2C, 2E, 2A2 or 10A were ... normal salivary glands It has therefore been suggested that T helper type (Th1) cytokines, such as IFN-γ and IL-2, as well as IL-10, IL-6 and TGF-β, might be important in the induction and/ or maintenance...

Ngày tải lên: 09/08/2014, 01:21

... discussions and Edith Trejo and Carlos Zadikian for technical assistance We are indebted to Drs J J Cazzulo and C Labriola for their generous gifts of a cruzipaincontaining plasmid and primers A and ... [10] In the present Foreign ADC gene expression in T cruzi work we used ADC-transgenic protozoa to follow the time-course modulation of foreign gene expression and to investigate whether this ... the parasite genome organization Results and Discussion Expression of the oat ADC gene in T cruzi In order to study the expression of the foreign ADC gene in T cruzi epimastigotes, we transformed...

Ngày tải lên: 19/02/2014, 07:20

... activation and NAMI-A- induced down regulation of c-myc gene expression NAMI-A inhibits c-myc gene transcription and protein expression To establish whether the decrease in c-myc mRNA expression ... regulates c-myc gene expression To investigate whether NAMI-A-induced inhibition of ERK1/2 signaling could affect the expression of a cell growth-related gene, we assessed c-myc gene expression in ... FEBS 2002 NAMI-A affects c-myc gene expression and MEK/ERK phosphorylation (Eur J Biochem 269) 5867 Fig NAMI-A and PD98059 completely inhibits PMA-induced c-myc gene expression (A) Serum-starved...

Ngày tải lên: 21/02/2014, 01:21

... pathways in which one or Gene Expression and Signal Transduction (A) Tryptophan operon 5′ DNA Regulatory gene i Transcription Promoter p Operator o 3′ Gene E Gene D Gene C Gene B Gene A Transcription ... on other genes Other important protooncogenes that encode nuclear transcription factors include MYC and MYB Both phytochrome (see Chap- Gene Expression and Signal Transduction ter 17) and gibberellin ... discussion of eukaryotic gene expression with a brief overview of transcriptional regulation in prokaryotes However, it is now clear that Gene Expression and Signal Transduction gene regulation in...

Ngày tải lên: 07/03/2014, 21:20

... regulating the gene expression of rate-limiting enzymes of gluconeogenesis and glycogenolysis, including phosphoenolpyruvate carboxykinase (PEPCK) and glucose-6-phosphatase (G6Pase), and by regulating ... cells, and the ensuing effect on gluconeogenesis and glucose output Our results show that, in the presence of TNFa, insulin-induced inhibition of gluconeogenesis and glucose output is attenuated and ... with TBP and b-actin antibodies and taken as nuclear and cytosolic loading controls, respectively, and also used to ascertain the purity of the nuclear and cytosolic preparations (A) Bands were...

Ngày tải lên: 16/03/2014, 02:20

... breakdown of ECM and facilitate cell invasion and metastasis Increased expression of uPAR is associated with invasive cancer cell phenotypes and a poor prognosis The ability to decrease uPAR expression ... decreased [9] We have previously cloned the human uPAR gene and characterized its promoter and transcription factors, including two AP-1 and one NF-jB motifs [10–12] We have also found that colon ... uPAR expression in colon cancer invasion and metastasis [13] To develop a potential tool for uPAR gene therapy, we used a catalytic DNA enzyme (DNAzyme) approach The DNAzymes were originally generated...

Ngày tải lên: 16/03/2014, 22:20

... metabolism and HBV gene expression H Y Kim et al the liver, intestine, kidney and adipose tissue via the regulation of the expression and function of genes involved in bile acid synthesis, uptake and ... metabolism and HBV gene expression Fig The effects of bile acids on HBV gene expression in hepatocyte cell lines (A) Chang liver, HepG2 and Huh7 cells were transfected with the 1.3x HBV-luc construct and ... metabolism and HBV gene expression Fig The effect of AP-1 and C ⁄ EBPs on bile acids-induced HBV gene expression (A) Chang liver cells were cotransfected with AP-1-luc or 3xC ⁄ EBP-luc construct and...

Ngày tải lên: 22/03/2014, 21:21

... SREBP target gene, and that the transcription of SREBP-1a gene is predominantly regulated by the general transcription factor Sp1 [11,12] We next compared the glutamineinduced gene expression of ... of SREBP gene expression is not the initial trigger for the glutamine effects If induction of the SREBP gene expression serves as the initial trigger for the glutamine effects, the gene expression ... in the induction of SREBP-1a gene expression When endogenous Sp1 expression was reduced to  20% of normal with gene- specific small interfering RNA (siRNA), the gene expression of SREBP-1a was significantly...

Ngày tải lên: 28/03/2014, 22:21