gene transfer genetic analysis and strain manipulation

... Finite element (FE) models General approach FE models of the Modular and Monoblock implants were developed, and strain and stress maps were generated for two constructs, A and B Construct A modeled ... Constructs A and B Meshing and material properties ANSYS Workbench 10.0 was used to generate meshes For Construct A, the number of nodes and elements was 41098 and 29436 (Modular) and 33270 and 23744 ... testing, and data analysis HB, RZ, and MM did the literature search, manuscript writing, figure preparation, and data analysis MP and PZ did extensive re-reading of the manuscript PZ and EHS were...

Ngày tải lên: 20/06/2014, 04:20

... History Survey, Champaign, Illinois, 1989 [22] Takahata N., Gene identity and genetic differentiation of populations in the finite island model, Genetics 104 (1983) 497- 512 [23] Toro M.A., Nieto B., ... value of using probabilities of gene origin to measure genetic variability in a population, Genet Sel Evol 29 (1997) 5-23 [3] Ellegren H., Johansson M., Sandberg K., Andersson L., Cloning of highly ... testing and linkage analysis in the horse using a set of highly polymorphic microsatellites, Anim Genet 25 (1994) 19-23 [16] Milligan B.G., Leebens-Mack J., Strand A.E., Conservation genetics:...

Ngày tải lên: 09/08/2014, 18:21

... of developing a non-viral gene delivery of anti-HIV genes for gene therapy, here we evaluated the utility of SB transposon system to deliver siRNA genes for stable gene transfer Two previously ... Results Stable gene transfer of CXCR4 and CCR5 shRNAs by SB transposon system To investigate the utility of SB mediated gene transfer of anti-HIV-1 coreceptor siRNAs against CCR5 and CXCR4 we used ... retro and lentiviral mediated gene transfer [37] Among these are that no viral sequences are involved thus minimizing insertion transcriptional activation of cellular genes and risk of generation...

Ngày tải lên: 10/08/2014, 05:21

Ngày tải lên: 13/11/2014, 09:37

... conducted detailed genetic analyses including homology analysis, phylogenetic analysis (using both the neighbor joining (NJ) and ML methods), and analysis of the pairwise distances of HN gene in HPIV1 ... on the phylogenetic tree was as follows: cluster 1, 84 strains including strains isolated during 2004–2009; and cluster 2, 95 strains from 2003 to 2005, and 2007 to 2009 Figure Phylogenetic tree ... Analyzer, Applied Biosystems, Foster City, CA) Phylogenetic analysis and calculation of pairwise distances by the NJ method Phylogenetic analysis of the nucleotide sequence of the HN coding...

Ngày tải lên: 18/06/2014, 18:20

... gene and its related functional and genetic studies 21 1.2.3 ABCB4 (MDR3) gene and its related functional and genetic studies 26 1.2.4 An important CYP3A cluster: the CYP3A4, CYP3A5, CYP3A7 and ... MDR1, MDR3 genes and the CYP3A cluster are introduced Previous genetic and pharmacogenetics studies on these candidate genes are also reviewed in this section Finally, the objectives and significance ... the non-random association between the genetic variations, which plays pivotal role in many genetic studies including Pharmacogenetics Linkage Disequilibrium rises and decays by mutation and recombination...

Ngày tải lên: 15/09/2015, 17:10

... radial and circumferential strain and strain rate by 2DST in normo-, hypo-, akinetic and dyskinetic segments of post-infarct patients 8-13, 30-37, which were not reliably accessible by TDI Radial strain ... Bijnens B Strain and strain rate imaging: a new clinical approach to quantifying regional myocardial function J Am Soc Echocardiogr 2004; 17(7): 788-802 Voigt JU, Flachskampf FA Strain and strain ... with no LE Comparing segments with LE ≥ 51% and LE 1-50% presented a significant difference of tissue velocities (S´ and E´), but not of strain and strain rate values In contrast to our results,...

Ngày tải lên: 25/10/2012, 11:15

... expression and activity in subcellular fractions of H9c2 cells overexpressing both MCD and AMPK Fig AMPK overexpression by adenoviral gene transfer and endogenous MCD activity (A) AMPK expression (i) and ... released into cytosol Western blot and SDS/PAGE for AMPK, MCD and mitochondrial markers To identify AMPK and MCD in the samples, SDS/PAGE and Western blot analysis was peformed Thirty micrograms ... transferred to a glass-Teflon homogenizer (Potter-Elvehjem, between 0.10 and 0.15 mm clearance), and homogenized by 10–15 up and down strokes while revolving at 500 r.p.m Homogenate was then transferred...

Ngày tải lên: 16/03/2014, 16:20

... Almeciga-Dıaz et al Promoter and SUMF1 effect on Morquio gene transfer Barrera conceived the study, its design and coordination, and helped to draft the manuscript All of the authors read and approved the ... Promoter and SUMF1 effect on Morquio gene transfer 48 Vargas J Jr, Gusella GL, Najfeld V, Klotman ME & Cara A (2004) Novel integrase-defective lentiviral episomal vectors for gene transfer Hum Gene ... C & Mannoni P (2000) Gene transfer to hepatocellular carcinoma: transduction efficacy and transgene expression kinetics by using retroviral and lentiviral vectors Cancer Gene Ther 7, 1286–1292...

Ngày tải lên: 29/03/2014, 21:20

... direction Oy, and , the shearing strain relative to Ox and Oy y Figure 5.14 Strains in an inclined direction; strains in the directions 0, and 0, and defined by E,, E, and, ,y lead to strains E , ... (ii) strain distribution (iii) stress distribution Figure 5.33 In-plane stresses and strains in a laminate 140 Analysis of stress and strain As the load P is in-plane and symmetrical, the strain ... 5.16(iii), is found by superposing the strains of Figures 5.16(i) and (ii); taking tensile strain as positive and compressive strain as negative, the strains in the x- andydirections are given, respectively,...

Ngày tải lên: 08/04/2014, 11:27

... marked for banding/collection reference Adenoviral Vectors: Production and Purification 17 References Graham, F L and Prevec, L (1991) Manipulation of adenovirus vectors, in Gene Transfer and Expression ... this case is defined both from the standpoint of genetic homogeneity, and from the absence of any toxic elements that may jeopardize cellular homeostasis and/ or virion-cell receptor interactions ... Production and Purification Adenoviral Vectors Production and Purification Faris P Albayya and Joseph M Metzger Introduction Current methodologies in first-generation adenoviral gene transfer, ...

Ngày tải lên: 10/04/2014, 11:11

... field-mediated DNA transfer Pm Natl Acad Sci USA 63,349~3499 Kaye, J and Hedrick, S M (1988) Analysis of specificity for antigen, MIS, and allogeneic MHC by transfer of T-cell receptor cx -and @hain genes ... cell and the presence of a human phenotypic trait is the basis of human gene mapping by somatic cell genetics (2) This method for constructing physical maps has been central to human genetics and ... somatic-cell genetics and molecular methods, we have explored two techniques that combine chromosome fragmentation with somatic cell genetics The first method, chromosome-mediated gene transfer (CMGT)...

Ngày tải lên: 11/04/2014, 09:42

... Montagu, M., and Schell, J (198 1) Site-specific mutagenesis of Agrobactertum Ti-plasmids and transfer of genes to plant cells J MoZ AppZ Genet 1, 149-164 Ditta, G , Stanfield, S , Corbm, D., and Helmski, ... cotransferred gene of interest (see Note 7) References Horsch, R B , Fry, J E., Hoffman, N L., Elchholtz, D , Rogers, S G , and Fraley, R T (1985) A simple and general method for transferring genes ... this gene inconvenient for sensitive quantification of gene expression The expression of the neomycin phosphotransferase (nptIl) (see Chapter 12) and phosphinothricin acetyltransferase (bar) genes...

Ngày tải lên: 11/04/2014, 10:10

... 2, 404–414 Heteroduplex Analysis in DNA Mutations 13 Analysis of Genetic Variants in Cardiovascular Risk Genes by Heteroduplex Analysis Ana Cenarro, Fernando Civeira, and Miguel Pocovi Introduction ... bands, two corresponding to the two different single-stranded DNA molecules and the remainder corresponding to the doublestranded Usually the fastest migrating band represents the double-stranded ... methodologies and limitations, in Genetics of Common Diseases (Day, I N M and Humphries, S E., eds.), BIOS Scientific Publishers, Oxford, pp 33–74 Cooper, D N and Krawczak, M (1993) Human Gene Mutation,...

Ngày tải lên: 11/04/2014, 10:39

... proteins and therefore its role in HIV-1 pathogenesis To better characterize the oligomerization of Vpu and the domains for this, we have used genetic, biochemical and biophysical methods, and two ... energy transfer (FRET) This non-radiative energy transfer between donor and acceptor fluorophores is critically dependent upon the distance and dipole orientations of the two partners, and is ... electrophoretic and pull-down assays Vpu oligomerization based on gel electrophoretic and pull-down assays The full-length R5-Vpu protein and its transmembrane and cytoplasmic domains were synthesized and...

Ngày tải lên: 18/06/2014, 18:20

... too resulted in enhanced gene delivery [37,42] Compared to the non -genetic methods, our genetically PTDtat modified vector has major advantages for two major reasons: 1) genetic modification allows ... using a genetic capsid modification strategy We genetically incorporated the sequences encoding the PTDtat peptide into the 3' end of the Ad5 fiber gene, rescued the modified viruses, and characterized ... enhanced gene delivery efficacy compared to unmodified Ad5 vectors To confirm that this expanded tropism was mediated by the genetically incorporated targeting motif PTDtat, we performed a gene transfer...

Ngày tải lên: 18/06/2014, 18:20

... (0,1); ⎩ where U and U are the dimensionless forms of T1 and T2 , respectively (3.3) 192 Heat Transfer - Mathematical Modelling, Numerical Methods and Information Technology 3.2 Heat transfer in a ... Numerical Methods and Information Technology Finally, β = u∞ = 20, it can be seen from figures 5(d) and 5(e) and figures 6(c) and 6(d) that neither of the solutions based on Eqs (4.1), (4.2) and (4.10), ... Patankar, Numerical Heat Transfer and Fluid Flow (Hemisphere Series on Computational Methods in Mechanics and Thermal Science) T & F / Routledge, 1980 W Dai and S Su, “A nonstandard finite difference...

Ngày tải lên: 20/06/2014, 01:20