gene therapy in cardiovascular disease

... Trang 2GENETIC MANIPULATION OF CARDIOVASCULAR TISSUEModulating Gene Expression in Cardiovascular Tissue Gene therapy can be defined as any manipulation of gene expression that influences disease ... use of gene therapy in animal models and in clinical trials 183 An Introduction to Molecular Medicine and Gene Therapy Edited by Thomas F Kresina, PhD Copyright © 2001 by Wiley-Liss, Inc ISBNs: ... 1CHAPTER 8Gene Therapy in Cardiovascular Disease VICTOR J DZAU, M.D., AFSHIN EHSAN, M.D., and MICHAEL J MANN, M.D. INTRODUCTION The explosive growth in understanding the changes in gene expression...

Ngày tải lên: 10/04/2014, 22:11

... by intrathymic injections and oraladministrations of adenoviral protein extracts or by neonatal administration of thevirus in utero, thereby increasing long-term expression and allowing readministra-tion ... addressing the underlying genetic defect in liver cells In addition, gene therapeutic strategies could theoretically be used to treat acquireddiseases such as viral infections of the liver Infections ... INTRODUCTION In the early years of gene therapy, the liver was not taken into consideration as a 153 An Introduction to Molecular Medicine and Gene Therapy Edited by Thomas F Kresina, PhD Copyright...

Ngày tải lên: 10/04/2014, 22:11

... cancer gene therapy Thus, gene therapy for thetreatment of cancer has been directed at (1) replacing mutated tumor suppressorgenes, (2) inactivating overexpressed oncogenes, (3) delivering the genetic ... translational initiation site or a ing site on the gene (Fig 10.4) This binding represents an antigene approach that would inhibit genetic information flow (DNA–RNA–protein) The antigeneapproach ... andtiming of activation of the CDK Thus, each of the eight distinct cyclin genes (Table10.1) regulate the cell cycle at its designated point by binding to CDKs and formingCDK/cyclin complexes Cyclins...

Ngày tải lên: 10/04/2014, 22:11

... transgene expression in the joint was high [48], but interestingly the transgene expression also increased with joint inflammation [49] Clinical efficacy of local gene therapy with recombinant ... gene therapy trials The number of gene therapy trials in RA is in sharp contrast to the more than 600 clinical trials of gene therapy in patients with cancer Although not a life-threatening disease, ... of proinflammatory cytokines, thereby reducing their circulating levels and abolishing a sensitizing effect on nạve joints A conflicting finding was the protection of the ipsilateral hind paw...

Ngày tải lên: 09/08/2014, 01:24

... imaging methods using fluo-rescence and bioluminescence were introduced and increasingly used for temporal and spatial monitoring of transgene expression [11-13] Bioluminescence imaging (BLI) using ... author gliomabioluminescence imaginggene therapyherpes simplex virus type 1 thymidine kinaseluciferase Abstract Background: Suicide gene therapy employing the prodrug activating system Herpes ... (during and after GCV therapy) of tumor volumes and integrated light intensity units within a region of interest (ROI) including all tumors were plotted against each other (Figure 5) Within the...

Ngày tải lên: 14/08/2014, 19:22

... puncture (CLP)-induced septic shock model Induction of sepsis is intrinsically more challenging than inducing endotoxemia since the immune challenge is episodic in nature Some of the guidelines for ... for in vivo, differential effects are present in Trang 30various vascular beds Thus vasoconstriction may predominate in certain organs (e.g intestines, kidney) (Spain et al., 1994a; Spain et ... structure, in which each monomer consists of a reductase domain and an oxygenase domain, separated by a short sequence (30 amino acids) for the attachment of the Ca2+-binding protein calmodulin In addition...

Ngày tải lên: 14/09/2015, 14:16

... observed in hearing after AAV vector-mediated gene therapy [11] or in visual function after BMT [12] or localized AAV vector-mediated gene therapy [13] and have not been evaluated after systemic administration ... bone was stained with hematoxylin and eosin (20 original magnification) The inner surface of the cortex is at the top and white arrows indicate osteocytes in the cortical bone. Trang 7in MPS I ... pathology in a dose-dependent fashion DISCUSSION The goal of this study was to determine if liver-targeted gene therapy could correct the clinical manifestations of MPS I in mice Neonatal gene therapy...

Ngày tải lên: 02/11/2022, 14:26

... hunting-tin protein are lower in the R6/1 mice compared to R6/2 [10] and that neuronal intranuclear inclusions (NIIs) containing the human transgene-encoded amino terminus of human huntingtin ... dopamine neurotransmission, and abnor-malities in cannabinoid signalling or modulation of dop-amine signalling or both have been implicated in a number of neurodegenerative diseases including ... encoding mutant huntingtin (HD) Although it is known that the coding region of CB1 is contained within a single exon in mice, rats and humans, the 5¢-untranslated region of the mouse gene remained...

Ngày tải lên: 16/03/2014, 18:20

... favoring the insurgents) “COIN win” includes “COIN win” and “mixed, favoring COIN force.” “Mixed, favoring COIN force” occurs only once in the eight COIN wins For details on outcome scoring and ... our findings to be the most interesting or illuminating, this section presents six findings identified as key in formulating successful COIN operations Effective COIN Practices Tend to Run in Packs ... contained the insurgency by attacking the ADF’s rear bases in the DRC and by developing special COIN units trained in mountain warfare Papua New Guinea, 1988–1998 Case Outcome: COIN Loss The insurgency...

Ngày tải lên: 06/03/2014, 09:21

... cooperation, including new Turkish investments in the KRG.18 Finally, there have been indications within the PKK of a rethinking of the value of armed struggle In an interview with the respected Turkish ... Partnership against PKK training camps and sanctuaries in northern Iraq, fearing that this could destabilize the Kurdish-dominated areas in northern Iraq, which were relatively stable in comparison ... understanding of its long-term political repercussions These admissions are contained in a series of interviews of leading Turkish military commanders involved in the fight with the PKK The inter-...

Ngày tải lên: 22/03/2014, 21:20

... manpower, time spent investigating and explaining incidents, lost stock value following a major incident, cost of installing "end-of-pipe" solutions rather than "designing -in" solutions In this book ... Permit - Safe Work Practices - Confined Space Entry - Line Breaking - Machine Guarding - Welding, Cutting, Brazing - Personal Protective Equip - Spill Prevention Training X X X X X X X X X X X X Emergency ... improvement in resources and efficiency is achieved by integration and not by improving each individual program and element • Draw on existing material If your company is actively involved in industry initiatives...

Ngày tải lên: 02/04/2014, 16:31

... enrolled Infectious disease control in child care helps prevent spread of infectious disease in the community Outbreaks of infectious diseases and intestinal parasites in young children in child ... certificate in pediatric first aid, including CPR; d) Pre-service training in health management in child care, including the ability to recognize signs of illness, knowledge of infectious disease ... with new information on inappropriate infant sleeping equipment, pacifier use and swaddling Standard 2.1.1.4: Monitoring Children’s Development/ Obtaining Consent for Screening – NEW Defines the...

Ngày tải lên: 12/02/2014, 11:20

... key indicators, including infant mortality and injury deaths In Mexico, children living in rural and indigenous communities experience worse health outcomes than those who live in cities And, in ... cut spending in other areas, or look more and more to private payers — including making people pay more out of their own pockets in order to maintain their existing health care system In Mexico, ... 35th in the leading causes of death in Mexico, it is now third.118 In Canada, while national incidence data are not available, type diabetes is increasingly being recognized as a disease affecting...

Ngày tải lên: 12/02/2014, 12:20

... drinking-water quality Several measures were adopted to improve drinking-water quality, including: • increasing the public water supply, with 85% of people connected in 2005; • legally enforcing ... gastrointestinal disease and more than 50 million cases of severe respiratory diseases are incurred each year through swimming and bathing in wastewater-polluted coastal waters (13) Infectious diseases ... measures, information dissemination, public awareness and health advice WHO GUIDELINES FOR DRINKING-WATER QUALITY WHO’s Guidelines for drinking-water quality (39) are the international reference point...

Ngày tải lên: 12/02/2014, 12:20

... preventing the spread of disease in their child care setting Assist providers in establishing, developing and promoting written policies regarding health and safety in their child care setting Help ... disease and injuries The training in prevention of infectious disease is estimated to be four hours This curriculum is designed to assist the trainer in meeting the requirement and in providing sufficient ... 22 •Eating/drinking or handling food •Feeding a child •Giving medication (particularly eye drops/ointment, etc.) •Playing in water that is used by more than one person •Toileting, diapering and...

Ngày tải lên: 12/02/2014, 12:20

... use closed piping systems for draining and venting flange and pump seal leaks), and from non-routine hydrocarbon containing equipment prior to maintenance; operations (maintenance, inspection) ... Using the same water for multiple tests; include the following: • Reducing the need for corrosion inhibitors and other chemicals by minimizing the time that test water remains in • Use of boiling ... containment; and • Developing, implementing, and maintaining a specific Emergency Management Plan providing emergence kinetics; • Removing or diluting the release and limiting the area measures to...

Ngày tải lên: 14/02/2014, 03:20

... of drinking and recreational waterborne diseases in humans in the United States In the spring of 1993 in Milwaukee, municipal drinking water that was within bacterial standards was contaminated ... Activity Trends in the United States (in millions of people)144 Activity Visit beach or waterside Swimming in natural waters Canoeing Kayaking Snorkeling or scuba diving Surfing Jet skiing 1994–1995 ... to contaminated drinking water or groundwater; or from diving, swimming, kayaking, canoeing or other activities in recreational waters.55,56 Recreational exposure usually occurs through ingestion,...

Ngày tải lên: 17/02/2014, 10:20

... during activities such as eating, drinking, playing, learning or working (and scavenging, in poor areas ) •Chemicals are present in the places (settings) where children spend most time: including ... THE SINGLE MOST EFFECTIVE MEANS OF PROTECTING CHILDREN AGAINST TOXICANTS Health care providers play a key role in: Identifying the problem Defining its determinants and characteristics Informing ... effects and may be present in paints, antiseptics, and certain fish •Pesticides may be present in fields, in food, or in water runoff which may be used for drinking and cooking Some pesticides have...

Ngày tải lên: 18/02/2014, 15:20

... Diseases housing zones, providing protective clothing to personnel, Animal disease- causing agents can spread rapidly, especially in and closing holes in buildings to keep out wild animals); intensive ... • Waste Management Maintain feed storage , transport and feeding systems in good working condition; Solid waste generated during poultry production includes waste • Maintain records of livestock ... avoiding land spreading of manure within these areas.17 Industrial Process Wastewater Poultry operations may generate effluents from various sources including runoff from poultry housing, feeding,...

Ngày tải lên: 21/02/2014, 01:20