gene therapy for vascular diseases
gene therapy for primary immune deficiencies a canadian perspective
... development of HIV Gene therapy for primary immune deficiencies GT has been a particularly attractive option for PID The genes responsible for many PID have been identified, the diseases have been ... continue to carry significant complications Gene therapy Gene therapy (GT), i.e the use of genetic material to modify cells, has been investigated for numerous conditions since the development ... Trang 1Gene therapy for primary immune deficiencies: a Canadian perspective Xiaobai Xu1, Chetankumar S Tailor2 and Eyal Grunebaum1,3,4* Abstract The use of gene therapy (GT) for the treatment
Ngày tải lên: 04/12/2022, 10:38
Báo cáo y học: " Low autocrine interferon beta production as a gene therapy approach for AIDS: Infusion of interferon beta-engineered lymphocytes in macaques chronically infected with SIVmac251" pot
... virus These results reveal potential pitfalls for future developments of gene therapy strategies of HIV infection Background Highly active antiretroviral therapy (HAART) effectively inhibits human ... infection could be increased by develop-ing a gene therapy strategy based on the modified produc-tion of IFN-β in genetically engineered lymphocytes [18] For this purpose, a retroviral vector derived ... observed, and these macaques maintained high CD4+ T-lymphocyte counts for at least 478 days [24] However, a gene therapy strategy for HIV infection would only be possible during the chronic phase
Ngày tải lên: 13/08/2014, 13:20
Báo cáo sinh học: "A dual function fusion protein of Herpes simplex virus type 1 thymidine kinase and firefly luciferase for noninvasive in vivo imaging of gene therapy in malignant glioma" ppsx
... Corresponding author gliomabioluminescence imaginggene therapyherpes simplex virus type 1 thymidine kinaseluciferase Abstract Background: Suicide gene therapy employing the prodrug activating system ... insufficient transgene delivery to tumor cells Therefore, this study aimed at developing a strategy for real-time noninvasive in vivo monitoring of the activity of a therapeutic gene in brain tumor ... used for repetitive and noninvasive quantification of HSV-TK/ GCV mediated cell kill in vivo. Conclusion: This approach may represent a valuable tool for the in vivo evaluation of gene therapy
Ngày tải lên: 14/08/2014, 19:22
Báo cáo sinh học: "The use of retroviral vectors for gene therapy-what are the risks? A review of retroviral pathogenesis and its relevance to retroviral vector-mediated gene delivery" pptx
... vectors for gene therapy-what are the risks? A review of retroviral pathogenesis and its relevance to retroviral vector-mediated gene delivery Donald S Anson*1,2,3 Address: 1 Department of Genetic ... clinical trial of γc mediated gene therapy for X-linked severe combined immunodeficiency (X-SCID) has proven the potential of retroviral mediated gene transfer for the treatment of inherited ... vectors for gene therapy Non-defective oncogenic retroviruses Non-defective, replication competent retroviruses are also associated with malignant diseases These viruses do not carry oncogene sequences
Ngày tải lên: 14/08/2014, 19:22
Báo cáo sinh học: "Human cytomegalovirus plasmid-based amplicon vector system for gene therapy" ppt
... 1 of 11 (page number not for citation purposes) Genetic Vaccines and Therapy Open Access Research Human cytomegalovirus plasmid-based amplicon vector system for gene therapy Kutubuddin Mahmood, ... demonstrates that a foreign gene can be expressed in the context of a HCMV amplicon viral stock in infected HF cells. To test the utility of the HCMV amplicon in gene therapy or gene delivery, we ... hybridizes to a sequences. Genetic Vaccines and Therapy 2005, 3:1 http://www.gvt-journal.com/content/3/1/1 Page 8 of 11 (page number not for citation purposes) and expressing foreign genes in infected
Ngày tải lên: 14/08/2014, 19:22
Báo cáo sinh học: "Silencing the epidermal growth factor receptor gene with RNAi may be developed as a potential therapy for non small cell lung cancer" pot
... Combining RNAi with this gene delivery system may enable us to develop RNAi for silencing EGFR into an effective therapy for NSCLC Published: 30 June 2005 Genetic Vaccines and Therapy 2005, 3:5 doi:10.1186/1479-0556-3-5 ... current gene transfer vector systems, inadequate weak promoters to drive transgene expression Therefore, so far, only three cancer gene therapy protocols had reached phase III trials before being ... cycle at 94°C for 2 min, and 45 cycles involving denaturation at 94°C for 10 s annealing at 53°C for 30 s and extension at 72°C for 40 s, followed by a final extension at 72°C for 10 min The
Ngày tải lên: 14/08/2014, 19:22
Báo cáo sinh học: " Clostridial spores as live ''''Trojan horse'''' vectors for cancer gene therapy: comparison with viral delivery systems" pptx
... to serve as "Trojan horses" for the delivery of genes for cancer therapy Indeed, clostridial spores that were genetically manipulated to harbour genes for cancerstatic factors, prodrug ... consist of sev-eral aspects: one is from its transgene that encodes prod-rug converting enzymes for suicide-gene therapy or cytokines for immuno-gene therapy These are essentially the same as the ... vectors for cancer gene therapy At present, there are various gene therapy vector systems under development against cancer However, due to the complexity of the solid tumours involving angiogenesis,
Ngày tải lên: 14/08/2014, 19:22
Human pluripotent stem cell derived cellular vehicles for cancer gene therapy
... as source for MSCs and NSCs in suicide gene therapy 113 5.1.1 Baculoviruses as gene therapy vectors for human cancer 113 5.1.2 HESC- derived MSCs and NSCs as gene delivery vehicles for 5.1.3 ... Trang 31compared to single gene expression or even the injection of two genes (Samaranayake 1.1.2 Gene carriers for cancer therapy Successful gene therapy relies largely on gene delivery efficiency ... system for cancer treatment is desirable Of all candidates, gene therapy to achieve therapeutic effects through transgene expression is a promising treatment Unfortunately, although gene therapy
Ngày tải lên: 09/09/2015, 10:08
development of new human stem cell derived cellular vehicles for glioma gene therapy
... vectors in gene therapy for glioma Finally, this study demonstrated, for the first time, that human embryonic stem cells can provide a potentially unlimited source for glioma gene therapy Using ... therapies for degenerative diseases such as diabetes and Parkinson’s disease Finally, human ESCs provide an unlimited supply of cellular vectors for novel ex vivo gene therapy After genetic modification ... CELLULAR VEHICLES FOR GLIOMA GENE THERAPY ZHAO YING NATIONAL UNIVERSITY OF SINGAPORE 2008 Trang 2DEVELOPMENT OF NEW HUMAN STEM CELL-DERIVED CELLULAR VEHICLES FOR GLIOMA GENE THERAPY ZHAO YING
Ngày tải lên: 11/09/2015, 09:00
The study of gene and protein vaccines for allergic diseases in mice
... 1THE STUDY OF GENE AND PROTEIN VACCINES FOR ALLERGIC DISEASES IN MICE TAN LI KIANG NATIONAL UNIVERSITY OF SINGAPORE 2007 Trang 2THE STUDY OF GENE AND PROTEIN VACCINES FOR ALLERGIC DISEASES IN ... treatments of these diseases are largely symptomatic treatments Allergen-specific immunotherapy has been shown to be a curative treatment for allergic diseases, but the underlying mechanisms for the efficacy ... Liew Lee Mei, Mdm Wen Hong-Mei for providing me technical assistance I would like to thank the Bioinformatics Group at the Nanyang Polytechnic, Singapore, for performing the statistical analysis
Ngày tải lên: 14/09/2015, 14:20
Baculovirus mediated gene delivery for glioma therapy
... 2005), among which are suicide gene therapy, genetic immunotherapy, tumor suppressor gene or oncogene approaches, and anti-angiogenesis gene therapy Trang 14Suicide gene therapy is one of the commonly ... vector for gene therapy It is impossible to obtain success in gene therapy without effective gene delivery systems that can achieve high levels of therapeutic gene expression in targeted cells Gene ... accounting for 73% of the approved glioma gene therapy clinical trials (Barzon et al., 2006) As an attractive candidate for suicide gene therapy, the diphtheria toxin A-chain (DTA) gene has been
Ngày tải lên: 01/10/2015, 11:39
Nanoparticles of biodegradable polymers for gene therapy of hepatitis b
... Gene delivery 1 4 2.1.1 Overview of gene therapy 1 4 2.1.2 Hurdles for gene therapy 1 5 2.1.3 Systems for gene delivery 16 2.2 Viral vectors for gene delivery……… 17 2.3 Non-viral vectors for ... lab to the gene delivery system Trang 26Chapter 2 Literature Review 2.1 Gene therapy 2.1.1 Overview of gene therapy Gene therapy is a rapidly advancing field with great potential for the treatment ... efficiency and targeting problems[57] 2.1.2Hurdles for gene delivery The first challenging hurdle for gene therapy is the short-lived nature of gene therapy The therapeutic DNA introduced into target
Ngày tải lên: 05/10/2015, 21:33
Identification and characterization of IFI30 as a glioblastoma specific promoter for glioma gene therapy
... target for selective gene therapy As gene therapy for GBM is localised there is minimum risk of systemic toxicity (Pulkkanen and Yla-Herttuala, 2005) Gene therapy is the transfer of exogenous genes, ... therapies for Glioblastoma 3 1.2 Gene therapy for GBM 4 1.3 Transcriptional Targeting 5 1.3.1 Tissue-specific promoters 7 1.3.2 Tumour-specific promoters 9 1.4 Vectors for Gene Therapy ... survival Generally there is no curative therapy for GBM and long-term control is rarely achieved with current therapies (Pulkkanen and Yla-Herttuala, 2005) Trang 151.2 Gene Therapy for GBM Although
Ngày tải lên: 22/10/2015, 21:20
Guidance for industry human somatic cell therapy and gene therapy
... by gene therapy given directly to the subject When the genetic manipulation is performed ex vivo on cells which are then administered to the patient, this is also a form of somatic cell therapy ... information intended to provide manufacturers with current information regarding regulatory concerns for production, quality control testing, and administration of recombinant vectors for gene ... cell therapy is the administration to humans of autologous, allogeneic, or xenogeneic living cells which have been manipulated or processed ex vivo Manufacture of products for somatic cell therapy
Ngày tải lên: 24/10/2019, 10:26
gene therapy for hematological disorders
... PROGENITOR CELLS AS TARGETS FOR GENE THERAPY 143 144 GENE THERAPY FOR HEMATOLOGICAL DISORDERS restoration of cellular and humoral immunity were documented after gene therapy Data showed a surprisingly ... otherwise standard MuLV vectors for gene therapy has not been successful Beyond these HEMATOPOIETIC STEM AND PROGENITOR CELLS AS TARGETS FOR GENE THERAPY 137 efforts, there are obviously major ... In vivo gene transfer is most appropriate for target cells that cannot REQUIREMENTS FOR GENE TRANSFER INTO HEMATOPOIETIC CELLS 135 TABLE 6.1 Relevant Targets and Applications for Gene Therapy...
Ngày tải lên: 10/04/2014, 22:10
gene therapy for liver disease
... hand, it is necessary for any vehicle used for in 156 GENE THERAPY FOR LIVER DISEASE vivo hepatic gene therapy to reach the liver efficiently For systemic application, the gene vectors are ideally ... attempted so far GENERAL PRINCIPLES FOR HEPATIC GENE THERAPY There are two basic approaches for gene transfer into hepatocytes: ex vivo and in vivo strategies (Fig 7.1) Ex vivo therapy requires ... expression of the therapeutic gene. Alternatively, different viral vectors as well as liposomes can be used for ex vivo gene transfer GENERAL PRINCIPLES FOR HEPATIC GENE THERAPY 155 Collagenase treatment...
Ngày tải lên: 10/04/2014, 22:11
gene therapy for hiv infection
... competes for an essential substrate or co-factor that is available in limiting amounts, or, for proteins that form multimeric complexes, the 270 GENE THERAPY FOR HIV INFECTION TABLE 11.1 Gene Therapy ... gene expression Thus, the antisense gene expression must be quantatively higher than the levels of HIV-1 gene expression for an antisense gene therapy strategy to be effective Standard gene therapy ... levels in HSCs Thus, retroviral-mediated gene transfer is currently the optimal gene transfer system available for use in HIV-1 gene therapy The 282 GENE THERAPY FOR HIV INFECTION limitations of retroviral...
Ngày tải lên: 10/04/2014, 22:11
Nanofiber covered stent for vascular diseases
... angiographic restenosis was associated with a lower rate of TLR (3.5% for SESs vs 18.5% for BMSs; 3.3% for PESs vs 12.2% for BMSs) More recently, the benefits of DESs have been confirmed in studies ... deposits the fibers on the surface, where the fibers deposit to form a thin, uniform mesh Electrospinning generates continuous, uniformed and long fibers, which have diameters down to nano-scale ... A) 35% DMF, B) 40% DMF, C) 45% DMF, D) 50% DMF, E) 15% Chloroform/Methanol (70:30), F) 20% Chloroform/Methanol (70:30), G) 25% Chloroform/Methanol (70:30), H) 15% HFIP and I) 20% HFIP solutions...
Ngày tải lên: 14/09/2015, 08:41
Gene Transfer Approaches for Gynecological Diseases pot
... suicide gene therapy Antiangiogenic gene therapy Virotherapy Virotherapy Measles virus Virotherapy Measles and mumps viruses Virotherapy Adenovirus AAV Delivery of p73 HSV-TK suicide gene therapy ... is forthcoming Antiangiogenic Gene Therapy Antiangiogenic gene transfer inhibits formation of neovasculature required for tumor growth and may also act by collapsing immature tumor-associated vascular ... phase I trial [36] Molecular Chemotherapy Molecular chemotherapy (a.k.a suicide gene therapy) is a strategy based on delivery of genes encoding a prodrug- MOLECULAR THERAPY Vol 14, No 2, August 2006...
Ngày tải lên: 22/03/2014, 11:20
nonviral vectors for gene therapy, methods and protocols
... vectors for gene therapy formed by self-assembly of DNA with synthetic block co-polymers Hum Gene Ther 7, 2123–2133 11 Kabanov, A V and Kabanov, V A (1995) DNA complexes with polycations for the ... Nonviral Gene Delivery Gert W Bos, Daan J A Crommelin, and Wim E Hennink Introduction The aim of gene therapy is to treat inherited or acquired genetic deficiencies (e.g., cystic fibrosis) or viral diseases ... refs 8–10 for typical results) The protocol can be used for all adherent cell types For suspension type cells, cells need to be spun down before changing the medium at a speed suitable for the...
Ngày tải lên: 11/04/2014, 09:59