gene therapy for cardiovascular disease

... development of HIV Gene therapy for primary immune deficiencies GT has been a particularly attractive option for PID The genes responsible for many PID have been identified, the diseases have been ... consequent to EVI1 activation after gene therapy for chronic granulomatous disease Nat Med 2010;16(2):198–204 doi:10.1038/nm.2088. 45 Kuo CY, Kohn DB Gene therapy for the treatment of primary immune ... Trang 1Gene therapy for primary immune deficiencies: a Canadian perspective Xiaobai Xu1, Chetankumar S Tailor2 and Eyal Grunebaum1,3,4* Abstract The use of gene therapy (GT) for the treatment

Ngày tải lên: 04/12/2022, 10:38

... AccessPrevalence and risk factors for cardiovascular disease among chronic kidney disease patients: results from the Chinese cohort study of chronic kidney disease (C-STRIDE) Jun Yuan1,2, Xin-Rong ... intervals for these risk factors were 3.78 (2.55–5.59) for age 45–64 years and 6.07 (3.89–9 47) for age≥65 years compared with age <45 years; 2.07 (1.28–3.34) for CKD stage 3a, 1.66 (1.00–2.62) for ... respectively (P for trend = 0.001) The same pat-tern was observed with cerebrovascular disease (P for trend < 0.001) and PAD (P for trend = 0.001) The propor-tions of MI, cerebrovascular disease and

Ngày tải lên: 04/12/2022, 16:02

... and thus the former is the vector of choice for future gene therapy trials The number of gene therapy trials in RA is in sharp contrast to the more than 600 clinical trials of gene therapy in patients ... the application of gene therapy in RA, and the feasibility of gene therapy was recently demonstrated in phase I clinical trials Keywords: arthritis, cytokines, gene therapy, genetic synovectomy, ... safety of local gene therapy Gene therapy in RA is still in its adolescence, and more clinical studies must be initiated if it is to mature It is, however, imperative that gene therapy studies

Ngày tải lên: 09/08/2014, 01:24

... Questionnaire; CBT: Cognitive Behavioural Therapy; CREDO: Cardiovascular Risk E-couch Depression Outcome; CVD: Cardiovascular Disease; ECG: Electrocardiogram; GAD-7: Generalised Anxiety Disorder Scale; ... people with less severe disease (i.e., either less severe depressive symptoms or cardiovascular risk factors) might be of benefit [12] For example, peo-ple at high risk for CVD (e.g raised cholesterol, ... participating, information on the randomisation process each participant will undergo Trang 4to either one of two interactive internet programs, confi-dentiality information and contact information for the

Ngày tải lên: 11/08/2014, 16:22

... Thus, CD34 progenitor cells are an essential ingredient for HIV gene therapy In view of the need for CD34 cells for HIV gene therapy as well as for other hematopoietic disorders, if one can pro-duce ... lineages thus paving the way for utilization of these cells for hematopoietic cell therapy [20,27-29] For the effective utilization of hES-CD34 cells for HIV gene therapy, a number of parameters ... possibilities exist for treating a number of genetic and immune system dis-orders [1] Many novel applications can be foreseen for hES cells in infectious disease research AIDS is a potential disease that

Ngày tải lên: 13/08/2014, 09:21

... immunodeficiency virus type 1 disease: evi-dence for drug safety, tolerance, and antiviral activity J Infect Dis 1996, 173:1107-1114. E: Exploring interferon beta for gene therapy of HIV infection ... infection could be increased by develop-ing a gene therapy strategy based on the modified produc-tion of IFN-β in genetically engineered lymphocytes [18] For this purpose, a retroviral vector derived ... observed, and these macaques maintained high CD4+ T-lymphocyte counts for at least 478 days [24] However, a gene therapy strategy for HIV infection would only be possible during the chronic phase

Ngày tải lên: 13/08/2014, 13:20

... Corresponding author gliomabioluminescence imaginggene therapyherpes simplex virus type 1 thymidine kinaseluciferase Abstract Background: Suicide gene therapy employing the prodrug activating system ... insufficient transgene delivery to tumor cells Therefore, this study aimed at developing a strategy for real-time noninvasive in vivo monitoring of the activity of a therapeutic gene in brain tumor ... used for repetitive and noninvasive quantification of HSV-TK/ GCV mediated cell kill in vivo. Conclusion: This approach may represent a valuable tool for the in vivo evaluation of gene therapy

Ngày tải lên: 14/08/2014, 19:22

... vectors for gene therapy-what are the risks? A review of retroviral pathogenesis and its relevance to retroviral vector-mediated gene delivery Donald S Anson*1,2,3 Address: 1 Department of Genetic ... γc mediated gene therapy for X-linked severe combined immunodeficiency (X-SCID) has proven the potential of retroviral mediated gene transfer for the treatment of inherited metabolic disease However, ... vectors for gene therapy Non-defective oncogenic retroviruses Non-defective, replication competent retroviruses are also associated with malignant diseases These viruses do not carry oncogene sequences

Ngày tải lên: 14/08/2014, 19:22

... 1 of 11 (page number not for citation purposes) Genetic Vaccines and Therapy Open Access Research Human cytomegalovirus plasmid-based amplicon vector system for gene therapy Kutubuddin Mahmood, ... demonstrates that a foreign gene can be expressed in the context of a HCMV amplicon viral stock in infected HF cells. To test the utility of the HCMV amplicon in gene therapy or gene delivery, we ... hybridizes to a sequences. Genetic Vaccines and Therapy 2005, 3:1 http://www.gvt-journal.com/content/3/1/1 Page 8 of 11 (page number not for citation purposes) and expressing foreign genes in infected

Ngày tải lên: 14/08/2014, 19:22

... Combining RNAi with this gene delivery system may enable us to develop RNAi for silencing EGFR into an effective therapy for NSCLC Published: 30 June 2005 Genetic Vaccines and Therapy 2005, 3:5 doi:10.1186/1479-0556-3-5 ... current gene transfer vector systems, inadequate weak promoters to drive transgene expression Therefore, so far, only three cancer gene therapy protocols had reached phase III trials before being ... cycle at 94°C for 2 min, and 45 cycles involving denaturation at 94°C for 10 s annealing at 53°C for 30 s and extension at 72°C for 40 s, followed by a final extension at 72°C for 10 min The

Ngày tải lên: 14/08/2014, 19:22

... to serve as "Trojan horses" for the delivery of genes for cancer therapy Indeed, clostridial spores that were genetically manipulated to harbour genes for cancerstatic factors, prodrug ... consist of sev-eral aspects: one is from its transgene that encodes prod-rug converting enzymes for suicide-gene therapy or cytokines for immuno-gene therapy These are essentially the same as the ... vectors for cancer gene therapy At present, there are various gene therapy vector systems under development against cancer However, due to the complexity of the solid tumours involving angiogenesis,

Ngày tải lên: 14/08/2014, 19:22

... as source for MSCs and NSCs in suicide gene therapy 113 5.1.1 Baculoviruses as gene therapy vectors for human cancer 113 5.1.2 HESC- derived MSCs and NSCs as gene delivery vehicles for 5.1.3 ... Trang 31compared to single gene expression or even the injection of two genes (Samaranayake 1.1.2 Gene carriers for cancer therapy Successful gene therapy relies largely on gene delivery efficiency ... have enabled the development of gene therapy, which introduces genetic material into cells for therapeutic purposes Originally, gene therapy was proposed to correct genetic disorders by transferring

Ngày tải lên: 09/09/2015, 10:08

... and robustness of the platform Particularly for a multifactorial disease like AD, these platforms provide excellent opportunity to gather high-density biological information related to different ... inhibition in an Alzheimer’s disease cell model 13th International Geneva/Springfield International Symposium on Advances in Alzheimer's Therapy, 26-29 Mar 2014, Geneva, Switzerland 4. Bahety ... stress, neuronal cell loss and NFT formation, rather than a single cause Several competing hypotheses have been put forward for explaining the cause of the disease as shown in Figure 1.2 Amongst

Ngày tải lên: 09/09/2015, 11:27

... therapies for degenerative diseases such as diabetes and Parkinson’s disease Finally, human ESCs provide an unlimited supply of cellular vectors for novel ex vivo gene therapy After genetic modification ... vectors in gene therapy for glioma Finally, this study demonstrated, for the first time, that human embryonic stem cells can provide a potentially unlimited source for glioma gene therapy Using ... CELLULAR VEHICLES FOR GLIOMA GENE THERAPY ZHAO YING NATIONAL UNIVERSITY OF SINGAPORE 2008 Trang 2DEVELOPMENT OF NEW HUMAN STEM CELL-DERIVED CELLULAR VEHICLES FOR GLIOMA GENE THERAPY ZHAO YING

Ngày tải lên: 11/09/2015, 09:00

... hand, it is necessary for any vehicle used for in 156 GENE THERAPY FOR LIVER DISEASE vivo hepatic gene therapy to reach the liver efficiently For systemic application, the gene vectors are ideally ... was the first recipient of liver-directed gene therapy She was homozygous for a mutation in the LDL receptor gene, result- 162 GENE THERAPY FOR LIVER DISEASE ing in the expression of a nonfunctional ... damages such as motor 168 GENE THERAPY FOR LIVER DISEASE FIGURE 7.6 Gene therapy for a1-antitrypsin (AAT) deficiency A plasmid that contains the full-length human AAT gene is encapsulated in small...

Ngày tải lên: 10/04/2014, 22:11

... 184 GENE THERAPY IN CARDIOVASCULAR DISEASE GENETIC MANIPULATION OF CARDIOVASCULAR TISSUE Modulating Gene Expression in Cardiovascular Tissue Gene therapy can be defined as any manipulation of gene ... READINGS Cardiovascular Gene Therapy Allen, MD Myocardial protection: Is there a role for gene therapy Ann Thorac Surg 68:1924–1928, 1999 Amant C, Berthou L, Walsh K Angiogenesis and gene therapy ... the gene, preventing the translation into protein Another form of gene blockade is the use FIGURE 8.1 Gene therapy strategies See color insert (A) Gene transfer involves delivery of an entire gene, ...

Ngày tải lên: 10/04/2014, 22:11

... end-stage renal failure [8] The prevalence of Fabry disease is estimated at : 40 000 for the classic form The incidence of the variant form of Fabry disease was found to be higher Screening of various ... to Fabry disease, small molecules capable of specifically rescuing misfolded enzyme proteins have been identified for Gaucher disease [20,21], Tay-Sachs and Sandhoff disease [22] (details for Gaucher ... weightÆday)1 for weeks [47] No apparent toxic effects were observed in transgenic mice treated with DGJ for 140 days, indicating that DGJ is well tolerated in mice ASSC therapy for Fabry disease in...

Ngày tải lên: 30/03/2014, 03:20

... PROGENITOR CELLS AS TARGETS FOR GENE THERAPY 143 144 GENE THERAPY FOR HEMATOLOGICAL DISORDERS restoration of cellular and humoral immunity were documented after gene therapy Data showed a surprisingly ... otherwise standard MuLV vectors for gene therapy has not been successful Beyond these HEMATOPOIETIC STEM AND PROGENITOR CELLS AS TARGETS FOR GENE THERAPY 137 efforts, there are obviously major ... In vivo gene transfer is most appropriate for target cells that cannot REQUIREMENTS FOR GENE TRANSFER INTO HEMATOPOIETIC CELLS 135 TABLE 6.1 Relevant Targets and Applications for Gene Therapy...

Ngày tải lên: 10/04/2014, 22:10

... competes for an essential substrate or co-factor that is available in limiting amounts, or, for proteins that form multimeric complexes, the 270 GENE THERAPY FOR HIV INFECTION TABLE 11.1 Gene Therapy ... gene expression Thus, the antisense gene expression must be quantatively higher than the levels of HIV-1 gene expression for an antisense gene therapy strategy to be effective Standard gene therapy ... levels in HSCs Thus, retroviral-mediated gene transfer is currently the optimal gene transfer system available for use in HIV-1 gene therapy The 282 GENE THERAPY FOR HIV INFECTION limitations of retroviral...

Ngày tải lên: 10/04/2014, 22:11

... Nanogene Therapy for Lung Disease with those from CIN-treated mice The gene array analysis was repeated once with a similar expression profile Ribonuclease Protection Assay for Cytokine Gene ... experiment Kong et al, Chitosan Interferon-c Nanogene Therapy for Lung Disease 103 Figure Chitosan interferon-c nanogene (CIN) treatment induces changes in gene expression in lung dendritic cells A, ... facilitate gene delivery to mucosa- and bronchus-associated lymphoid tissue Chitosan, therefore, appears to possess all of the attributes of an ideal gene delivery agent for effective nonviral gene...

Ngày tải lên: 08/08/2014, 21:20