functional role of iron

... a functional role for each of the membrane-proximal domains of gp130 for receptor activa-tion in response to ligands that lead to gp130 homodime-rization We proposed a particular role for D5 of ... 4 of gp130 can be replaced by a similar domain of a different receptor without abrogation of signal transduction, this points to a spacer role of the domain Intriguingly, signal transduction of ... unchanged Replacement of D6 of gp130 by the corresponding domain of the GCSFR leads to the abrogation of signal transduction of all tested cytokines As coprecipitation of this gp130 mutant and

Ngày tải lên: 18/03/2014, 01:20

... may act as an allosteric inhibitor of m-NAD-ME [18,21,25], and the allosteric properties of this isoform may relate to its particular role in the pathways of malate and glutamine oxidation in ... K0.5,Malatevalues of E59D were 2-fold and 4.4-fold higher, respectively, than that of the wild-type Furthermore, the kcat value of E59 mutant enzymes was only one-third to one-quarter of that of the wild-type, ... Multiple sequence alignments of three clusters of ME isoforms around the fumarate-binding region are shown Amino acid sequences of MEs were obtained by a similarity search of BLAST [44], and alignments

Ngày tải lên: 23/03/2014, 06:20

... the role of the linker region In addition, we fur-ther analyzed the functional role of the linker region based on the crystal structures of a P-gp homolog, MsbA [28] Results Protease treatment of ... be used to perform kinetic analy-ses of the native and linker-cleaved P-gp In the present study, we investigated the functional role of the linker region of human P-gp using a highly purified ... transports a wide variety of struc-turally unrelated compounds out of cells P-gp possesses a ‘linker region’ of 75 amino acids that connects two homologous halves, each of which contain a transmembrane

Ngày tải lên: 29/03/2014, 23:20

... rate of polymerization of both fibrin desAABB and fibrin produced in the fibrinogen + thrombin reaction (Fig 6A,B) The values of IC50(the concentrations of the peptides at which 50% of inhibition of ... region of the molecule To verify this suggestion, we investigated the inhibitory action of Fig 1 The dependence of the maximum rate of fibrin desAABB polymerization (Vmax) on the molar ratios of ... fragment of the fibrin Bb-chain This site is operational before fibrinopeptide B is split off The inhibitory activity of synthetic peptides mimick-ing the various fragments of the N-terminal region of

Ngày tải lên: 30/03/2014, 03:20

... disrupted by insertion of kan r into the PstI site Transformation of strain 2951 resulted in allelic replacement (B) Construction of plasmid for mutation of lgt1 and dele-tion of lgt4: lgt1 and flanking ... insertion of kan r into the StyI site Transformation of strain 2951 resulted in recombination within lgt5 and lgt1, resulting in deletion of lgt4 and disruption of lgt1 (C) Construction of plasmid ... Transformation of strain 2951 resulted in recombination within lgt5 and lgt1, resulting in deletion of lgt4 and disruption of lgt5. Trang 6selective TOCSY experiments Although the anomericsignals of each of

Ngày tải lên: 30/03/2014, 08:20

... major role in the survival of an agent Pleasure and pain are not properties of the environment Our brain generates pleasant or unpleasant emotions in response to those aspects of the environment ... emotions, in terms of their evolutionary origin; their basic neurobiological substratum, and their functional significance in autonomous agents, we propose a model of minimal functionality of emotions ... Trang 1The functional role of emotions in aesthetic judgmentIoannis Xenakisa, Argyris Arnellosb,*, John Darzentasa a Department of Product & Systems Design Engineering, University of the Aegean,

Ngày tải lên: 30/03/2014, 16:20

... structure of apoE and the meta-analysis on the populations of people with apoE isoforms 7Figure 1-3 The structure of LDL receptor family members 16Figure 1-4 Schematic representation of the canonical ... abnormal assemblies of β-amyloid (Aβ) and neurofibrillary tangles (NFTs) composed of hyperphosphorylated forms of microtubule-associated tau protein, as well as the loss of neurons and synaptic ... are of particular interest due to their essential role in Wnt signaling, dysregulation of which has been reported to be associated with AD Given the lack of information between the involvement of

Ngày tải lên: 09/09/2015, 08:16

... SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF BIOCHEMISTRY NATIONAL UNIVERSITY OF SINGAPORE 2007 Trang 3I would like to thank my supervisors, Prof Kam Hui and A/Prof Kanaga Sabapathy ... 109 List of Figures Figure 1-2 Development of the cervical transformation zone 5 Figure 1-3 Diagram of extent of spread in FIGO staging of cervical cancer 11 Figure 1-5 Dual effect of HPV E6 ... over-expression of p16 is functionally sequestered and cell cycle progression is unaffected (38) The up-regulation of p16 is in stark contradiction to its role as a tumour suppressor, and as yet, the role of

Ngày tải lên: 14/09/2015, 11:19

... induction of apoptosis, transfection of anti-miR-10b resulted in a dose-dependent induction of apoptosis (Fig 3b), again suggestive of sensitization of these cells to tamoxifen through deregulation of ... 1R E S E A R C H A R T I C L E Open AccessFunctional role of miR-10b in tamoxifen resistance of ER-positive breast cancer cells through down-regulation of HDAC4 Aamir Ahmad1, Kevin R Ginnebaugh1, ... Collectively, these results provided a clear functional involvement of miR-10b in tamoxifen resistance HDAC4 is a novel target of miR-10b Having established a role of miR-10b in tamoxifen resist-ance,

Ngày tải lên: 28/09/2020, 10:19

... Conformational stability and functionality of WT and mutant opsins One of the important aspects underlying the functionality of rhodopsin in visual perception is the structural stability of both the dark-adapted ... importance of molecular investigations of positive selected sites in rhodopsin evolution and the relevance of structural and functional analysis of these sites in unravelling the molecular basis of visual ... needed to determine the role of specific amino acid substitutions on the structure and function of these visual proteins during their evolution1 One of the main phenotypic marks of visual opsins is

Ngày tải lên: 04/12/2022, 10:31

... Functional expression of human liver cytosolic b-glucosidase in Pichia pastoris Insights into its role in the metabolism of dietary glucosides Jean-Guy Berrin 1,2 , ... (glycosphingo- lipids) present in the lysosomal membranes, a nd a lack of this enzyme is the cause of the various form s of Gaucher's disease, one of the hereditary lysosomal storage disorders [3]. Lactase-phlorizin ... contain an ORF of 1407 nucleotides encoding a p rotein of 496 a mino acids with a calculated molecular mass of 53.7 kDa. A single putative glycosylation site was located at N47 of the deduced

Ngày tải lên: 08/03/2014, 16:20

... presence of NaTDC (data not shown) In the case of NcC2, no inhibition of the lipase activity was observed, as 100% of the lipase activity still remained even at a molar excess of 45 The effect of NcC2 ... regard to the rate of inactivation show for the first time the dominant role of the N-terminal domain of PL in the phenomenon of interfacial dena-turation The N-terminal domain of PLRP2 does not ... understanding of the role of the C-termi-nal domain in the function of PLRP2, mainly with regard to the orientation of the residues essential for col-ipase binding and the behavior of PLRP2 towards

Ngày tải lên: 16/03/2014, 06:20

... determining short-termfunctional outcomes following a wide range of injury types The underlying hypothesis of this investigation is that, aside from the nature of injury, a range of other factors ... as a score of ≤ 25 out of a maximum possible score of 40 Indication of a major depressive episode was assessed by using two DSM-III screening questions for depressed mood or loss of interest ... one night or more as a result of their injury Participants were also asked for their assessment of the seriousness of the injury in terms of threat to life and threat of dis-ability The questions

Ngày tải lên: 20/06/2014, 15:20

... (PBMC) of mucocutaneous Figure 1 Qa-2 expression in PBMC of BD A RT-PCR demonstrated that mRNA expression was lower in PBMC of BD skin than in BD normal mice B The frequency of Qa-2 in PBMC of normal ... injection of siRNA into BD mice down-regulated the frequency of Qa-2 expression in lym-phocytes and granulocytes of BD mice Treatment of BD mice with Qa-2 siRNA resulted in deterioration of symp-toms ... believed to play roles in the immunopatho-genesis of Behcet's disease [46,47] Functionally, HLA-G directly inhibits the cytolytic function of peripheral blood NK cells [48] The frequency of NK cells

Ngày tải lên: 11/08/2014, 03:20

... regulation of functional categories in response to alterations of iron homeostasis in Arabidopsis thaliana Mara Schuler1, Andreas Keller2, Christina Backes2, Katrin Philippar3, Hans-Peter Lenhof2and ... and statistical analysis of the microarray data CB conducted adaptations of the GeneTrail software for the use of Arabidopsis thaliana CB and AK supported the application of GeneTrail and revised ... identify functional categories in large-scale plant transcriptome data sets The distinguished feature that allowed analysis of individually assembled functional categories facilitated the study of

Ngày tải lên: 11/08/2014, 11:20

... article Functional analysis of B and C class floral organ genes in spinach demonstrates their role in sexual dimorphism Abstract Background: Evolution of unisexual flowers entails one of the ... USA Full list of author information is available at the end of the article Trang 2Among the most extreme examples of evolution of thereproductive organs in flowers is the development of uni-sexual ... contrast, the assumption of the gen-eral conservation of expression has been used to assign homology of highly derived organs to putative ancestral forms [10-14] Lastly, evolution of the coding sequences

Ngày tải lên: 12/08/2014, 03:21

... expression (Sun et al., 2002) In view of all these interesting examples of intracellular CD38, the present study was carried to characterize the functional role of specific organelle targeted-CD38 ... regions of CD38 expressed co-localization with DioC6 and other regions where co-localization was not evident The partial merge intensity profile may be a result of the accumulation of non-functional ... no direct involvement of ectocellular synthesis of cADPR on the regulation of the cADPR-mediated intracellular Ca2+signaling in T-lymphocytes and observed no increase of intracellular cADPR

Ngày tải lên: 11/09/2015, 09:07

... cerebrum, the association of immunoreactivity with synaptic vesicles is rather unique to cerebellar neurons, suggesting an additional functional role of CD38 in certain types of presynaptic region ... (Pitter et al., 2002) In the event of high local Ca2+ domains of tens of micromolar in the vicinity of voltage-operated Ca2+channels or Ca2+ release sites of the endoplasmic reticulum (Rizzuto ... show the functional role of CD38 on mitochondria Here, for the first time, enzymatically active CD38 was shown to be present intracellularly on the outer mitochondrial membrane (OMM) of CD38+mitochondria

Ngày tải lên: 11/09/2015, 09:08

... ratio of NAD+glycohydrolase activity as compared to ADP-ribosyl cyclase activity (Chapter 4 Section 4.2.4.2) In view of the well established functional role of CD38, that is the hydrolysis of NAD+ ... molecules may have specific role against each other The roles of nitric oxide in the enzyme regulation such as the autoribosylation of CD38 or regulation of the ADP-ribosylation of other proteins via ... post-ischemic/hypoxia model of brain in vitro system can be established as future study to characterize the functional role (s) of brain mitochondrial CD38 A study of CD38KO mice (Jin et al.,

Ngày tải lên: 11/09/2015, 09:08

... store This data suggests a functional role of mitochondrial CD38 in Ca2+ signalling Having observed the functional role of CD38 in the overexpression system, it is of interest to verify this ... Determination of the purity of Percoll purified brain mitochondria 161 4.2.3 Determination of the submitochondrial localization of CD38 166 4.2.4 Determination of the enzymatic activities of brain ... an analog of NAD+ with guanine substituting for the adenine group, to produce cGDPR, a fluorescent analogue of cADPR and the site of cyclization is N7 of the guanine ring instead of N1 of the adenine,

Ngày tải lên: 11/09/2015, 09:08