functional anatomy of the hip and pelvis

... polymorphism at these loci and the presence of DDH and PPA We also explored the effect of genotype on acetabular morphology and severity of the conditions Patients and methods Hypothetically, it ... drawn from the centre of the head to the lateral edge of the acetabulum on antero-posterior radiographs of the pelvis [25] The acetabular index was measured as described by Sharp, between the inter-tear ... laxity and the other affecting the shape of acetabulum to be responsible for the causation of DDH Carter and Wilkinson reported increased incidence of joint laxity with DDH in 1964 [32] With the...

Ngày tải lên: 11/08/2014, 08:21

... determine the effects of GS compared with placebo during the first six weeks of the intervention and the effects of the walking program without the use of GS during the remaining weeks Furthermore, the ... groups on the mean number of days walked during each of the 12 weeks of the Stepping Out program and on the mean number of steps walked per ‘walking’ day during that time Type and usage of rescue ... participated in the interpretation of the data and the drafting of the manuscript, and all authors read and approved the final manuscript Competing interests All authors declare that they have no...

Ngày tải lên: 12/08/2014, 11:23

... II The hood domain is mainly built up by residues from the C-terminus and consists of a two-stranded anti-parallel b-sheet composed of b2 and b9 and an a-helix from the C-terminus (Fig 1A) The ... Lys173 interacts with both O6 and N7 (Fig 1C) The 2¢OH of the ribose is hydrogen bonded to the main chain and the side chain of Asp142 (Fig 1C) Both hydroxyl groups of the ribose are involved in ... Asp142, 2¢ and 3¢OH of the ribose and three water molecules with ˚ coordination distances of approximately 2.4 A The phosphate of the GMP is hydrogen bonded to main chain and side chain atoms of amino...

Ngày tải lên: 15/02/2014, 01:20

... a4-helix to the b4 strand and the b9 strand to the b10 strand at the bottom of the Zn-stem loop in the pRN1 prim–pol structure However, because the Zn-stem loop is a fairly self-standing structure ... 70 and 80 °C between 190 and 260 nm with a step increase of 0.2 nm, and a bandwidth of nm Thermal stability of Rep245 was measured by incubating the protein at 60, 70 and 80 °C for 30 and then ... the two-metal-ion mechanism of both RNA and DNA synthesis [17] In a previous study, we reported the findings of an analysis of the complete sequence of the cryptic plasmid pIT3 isolated from the...

Ngày tải lên: 18/02/2014, 18:20

... the C-terminal domain of apoCII interacts with the interface of the N- and C-terminal domains of LPL and part of the lid surface [32] Because the functional importance of LPL’s C-terminal domain ... illustrates the overall saddle shape of the dimeric structure and the key heparin-binding residues in the N-terminal domain held coordinately by the two subunits on the top of the hollow (Fig 1A) The ... not the lipase activity and that the normal affinity of these mutants for heparin (Fig 3, Table 1) implies conservation of the overall structure of the WW and WWW mutants – indicate that these...

Ngày tải lên: 21/02/2014, 15:20

... interaction of the membrane-bound AAA complex and the importomer Therefore, the protein complexes isolated with protein A fusions of Pex1p, Pex6p and Pex15p were tested for the presence of peroxins of the ... contaminate the eluates (Fig 3A and data not shown), thereby confirming the specifity of the isolation Taken together, these data indicate a close association of the membrane-bound AAA complex and the ... Moreover, these data indicate that at least one of the contact sites of the AAA complex and the importomer is made by Pex15p To investigate the influence of individual peroxins of the importomer on the...

Ngày tải lên: 07/03/2014, 12:20

... to the cytosol Here we confirm and extend earlier studies of these AAA-peroxins and give a further detailed functional analysis of their cassette structure and interaction The interaction of Pex1p ... of Pex1p and Pex6p A B Fig Two-hybrid interaction of Pex1p and Pex6p harboring point mutations of the WalkerA and B motifs of their ATP-binding sites A schematic representation of the Pex1p and ... the binding sites of these two AAA-proteins to their different protein regions and elucidated the importance of ATP-binding to the two AAA-cassettes of Pex1p and Pex6p for their interaction The...

Ngày tải lên: 07/03/2014, 16:20

... on the one hand and lipid membranes with little incorporated protein on the other [48] The success of this method thus probably depends on the relative affinities of interaction of the protein and ... was present on the surface of transfected cells, and had the expected molecular mass To test whether the presence of the myc-his tag at the C-terminus of CCR5 would interfere with the protein’s ... glycosaminoglycans [37] As the myc-his tag of the protein was present at the C-terminus of the receptor which is located on the inside of the cell, we then determined if signal transduction by the protein was...

Ngày tải lên: 08/03/2014, 09:20

... of the medium and K2 the orientation factor, determined by the mutual spatial orientation of the transition dipole moments of the donor and acceptor As no data on the spatial orientation of the ... in cells of the inner hypodermis and the epithelium of the oviduct as well as extracellularly in the pseudocoelomic cavity of the worms Discussion A bundle of four a-helices constitutes the secondary ... determined pI of 6.1 and the theoretically deduced pI value of 6.22 was found A theoretical prediction of the secondary structural organization of Ag-NPA-1 performed on the basis of the amino acid...

Ngày tải lên: 16/03/2014, 18:20

... significantly in the presence or absence of the b1B subunit, whereas, under the same conditions, the rate of inactivation was increased in the presence of the b1 subunit The effects of b1B were further assessed ... in Fig 4A–D, the rates of activation and inactivation of the sodium current via 4768 N Qin et al (Eur J Biochem 270) Ó FEBS 2003 Fig The effect of b1B and b1 subunits on the function and expression ... part of intron adjacent to exon (Fig 1C and Table 1) As the site of divergence between the b1 and b1B subunit cDNAs was located precisely at the exon 3/intron boundary of the SCN1B gene, the human...

Ngày tải lên: 16/03/2014, 23:20

... from Hanes plots Determination of the metal contents of the Qor proteins, and detection of the nucleotide moiety of the molybdenum cofactor The contents of molybdenum and iron were determined by ... cleaves 364-bp upstream of the start codon of qorM, and DraIII, that cleaves 8-bp downstream of the stop codon of qorL After removing the 3¢ overhang and filling the 5¢ overhang of the plasmid with T4 ... indicated in Fig 4B The absence of Mo(V)signals reveals the g2-component of FeSII It is noted that the g-factors of the FeSI and II signals of the Qor proteins from the wild-type strain and from P putida...

Ngày tải lên: 17/03/2014, 10:20

... further uptake and preserving or enhancing their excretion The discovery of bile acids as ligands of the orphan liver receptor FXR [14] led to the concept of FXR-mediated regulation of Ntcp and Bsep ... Analysis of the 5¢ flanking region sequence of the Bsep gene A total of 2488 bp upstream from the 5¢-end of the Bsep cDNA was sequenced in both directions; 1583 bp are shown in Fig Identity with the ... even below that of the wild-type p-126 Luc Analysis of the binding affinity of the putative FXRE site for the orphan nuclear receptor FXR To characterize the FXRE site identified in the Bsep promoter...

Ngày tải lên: 17/03/2014, 23:20

... activity of PAF Differences and similarities between the structures of PAF and AFP The common geometrical arrangement of the two proteins is the special Greek key fold, in which the Structure and ... mPAF at the respective concentrations of and 100 lgỈmL)1 Fig Ribbon diagram of the mean PAF structure without disulfide bond constraints the core of the protein (Fig 9) As a consequence of the highly-twisted ... contributing to the sheet stabilization (Table 2) The b5 strand is the most negativelycharged region of PAF and close to the C-terminus starts at Ala51 Loop (47–50) connects strands b4 and b5 and creates...

Ngày tải lên: 29/03/2014, 23:20

... found in the father or the mother of 30 of these 125 HCM-patients, that could thus be regarded as familial cases In 45 patients, none of the parents were available for study A total of 40 of the 245 ... measured, and the left ventricular wall thickness (LVWT) calculated as the sum of IVS and PWT Table summarizes the main characteristics of patients All them fulfilled the next inclusion criteria: they ... of 150 of these controls were examined through electrocardiography to exclude the existence of cardiac diseases All the patients and controls were Caucasians from the Northern Spain regions of...

Ngày tải lên: 18/06/2014, 16:20

... follicles were cut open and the theca and adherent granulosa cells peeled from the stroma The granulosa cells were then gently scraped from the theca and the granulosa and theca cells were snap ... [21,22] The objectives of the studies reported here were to examine the interactions of the gonadotrophins and IGF with the Akt and Erk signalling pathways in theca and granulosa cells in vitro and ... concentration of 50 μM of the inhibitors, and 50 μM (0.005%) of the DMSO Concentrations of the inhibitors were based on the treatments used in vitro in Experiment The ewes recovered from surgery and 48...

Ngày tải lên: 20/06/2014, 07:20

... deciphering the structural and functional organization of the synapse We have assembled leaders in the field of synapse biology to describe and distill the wonders and mysteries of the synapse ... must occur within the tiny space of the synapse, the precise molecular organization of proteins, lipids, and membranes at the synapse is paramount Given the central role of the synapse in neuronal ... STRUCTURAL AND FUNCTIONAL ORGANIZATION OF THE SYNAPSE STRUCTURAL AND FUNCTIONAL ORGANIZATION OF THE SYNAPSE Edited by Johannes W Hell University of Iowa Iowa City, IA, USA and Michael D Ehlers...

Ngày tải lên: 06/07/2014, 15:30

... subcloned into the plasmid pGEM-3Z to generate the clone pGS217 and used for analysis and sequencing 2.2 Fusions of the 5’region of GS1a to the GUS reporter gene The 981 bp sequence upstream of the translation ... [4,16,18], and none of them correspond to nitrogen metabolism We have studied the promoter activity of the 5’-untranslated region using fusions with the reporter gene uidA and the presence of DNA-protein ... tissues of developing seedlings and the level of the transcript was affected by developmental and light conditions [9] Here, we present the DNA sequence of a partial genomic clone containing exons of...

Ngày tải lên: 08/08/2014, 14:20

... manuscript and its revision SG wrote with MM the statistical section of the protocol, checked the data and performed the statistical analysis IK performed the follow-up of the trial and the radiographic ... 12:515-524 Lequesne MG, Laredo JD: The faux profil (oblique view) of the hip in the standing position Contribution to the evaluation of osteoarthritis of the adult hip Ann Rheum Dis 1998, 57:676-681 ... report form The mark was then removed by the reader For the oblique view, measurement had to be performed in the anterior and upper part of the circumference between the femoral head and the acetabulum,...

Ngày tải lên: 09/08/2014, 07:20

... synovial fluid are the presence of hyaluronic acid and the ph of synovial fluid [8] On the other hand mechanical destruction of joint may be caused by several factors Firstly the initial trauma ... with a history of progressive, severe hip pain ten years after a gun shot injury to his right hip Radiographs at the time of injury confirmed the presence of bullet around the hip joint He was ... disorganization of the columnar epithelium Tide mark duplication and unequal thickness of the cartilage was observed in the articular cartilage while the synovium showed both cellular and stromal...

Ngày tải lên: 11/08/2014, 10:23

... drugs (Hofmann et al., 1999) 1.1.2 The β and α2δ subunits Auxiliary subunits of calcium channels modulate the trafficking and the biophysical properties of the α1 subunit The β subunit aids in the ... Alignment of the C-terminal sequences of the L-type CaV1.3 with CaV1.2 and CaV1.4 shows high conservation in the EF-hand and IQ motif (Figure 1.1) Alternative splicing of the CaV1.3 in the C-terminus ... like to thank all the members, past and present, of the Ion Channel and Transporter Laboratory for their support, encouragement and friendship Of special mention are Ms Yu Dejie and Dr Gregory Tan...

Ngày tải lên: 10/09/2015, 15:48