expression purification and immobilization of extremozymes

... downstream of the T7lac promoter and transformed into E coli BL21-(DE3) Recombinant strains were cultivated on 2YTA medium and induced by the addition of 1 mM of IPTG at 37 °C for 20 h Amplification of VEGF ... extraction protocols of recombinant VEGF Figure 3a shows that when only Fig 1 Analysis of electrophoretic profiles a Agarose gel profile of products resulting from PCR amplification of the VEGF transcripts ... proliferation Fig 2 Effect of culture conditions on the expression of VEGF165 in the pellet (insoluble) and the supernatant (soluble) of centrifuged crude lysates a Insoluble VEGF165 expression at different...

Ngày tải lên: 24/11/2022, 17:53

... syringaldazine[37] Kinetics of laccase revealed a Kmof 0.5 mM and 2.0 mM and Vmax of 285 U/mg and 500 U/mg by free and sol–gel immobilized laccase, respectively Km and Vmax values of immobilized laccase ... than free enzyme which was optimum at 50 °C and pH 6.0 Kinetic analysis showed Kmof 0.5 mM and 2.0 mM and Vmaxof 285 U/mg and 500 U/mg by free laccase and sol–gel immo-bilized laccase respectively ... 1ORIGINAL ARTICLEPurification and immobilization of laccase from Trichoderma harzianum strain HZN10 and its application in dye decolorization Zabin K Bagewadia,b,*, Sikandar I Mullaa,*, Harichandra Z...

Ngày tải lên: 04/12/2022, 16:06

... PCR. Of course one potential disadvantage of the increased number of PCR cycles is the increased possibility of accumulating PCR-mediated mutations in the final PCR products. Use of ‘proofreading’ ... primers. The positioning of the restriction site in relation to the 5′-end of the primer and the enzyme you choose dictate the efficiency of digestion and the overall success of your cloning experi- ... preventing DNA damage of the long molecules and enhancing isolation of full-length clones The range of cloning kits and vectors... normal dNTPs, therefore has one strand carrying terminal...

Ngày tải lên: 25/10/2013, 22:20

... observed when Assembly of VLPs by expression of RVFV N and Gc proteins Figure 5 Assembly of VLPs by expression of RVFV N and Gc proteins The supernatant of cells expressing N and Gc was purified ... http://www.virologyj.com/content/5/1/82Detection of RVFV Gn and Gc and the cell surface expression Figure 2 Detection of RVFV Gn and Gc and the cell surface expression A) Cell lysate from infected cells ... form complex structures To determine the posi-Expression and purification of RVFV nucleoprotein (N) protein Figure 1 Expression and purification of RVFV nucleoprotein (N) protein Insect Sf9 cells...

Ngày tải lên: 20/06/2014, 01:20

... thankful to Professor Les Copeland (Faculty of Agriculture, Food and Natural Resources - The University of Sydney, New South Wales, Australia) and Professor Kaeko Kamei (Department of Biomolecular ... subunits Aa,Bb and g are 95, 56, and 51.5 kDa (UniProt), respectively. The time of hydrolysis is plotted at the top of the figures. Table 3 The values of pI and molecular weights (MW) of all P.excavatus ... Generally, the tw o-step chromatography of AEX and HIC was sufficient for the purification of FIII-1, FIII-2, and FII, since their SE C and SDS-PAGE profiles repre- sented pure proteins. Fraction...

Ngày tải lên: 20/06/2014, 23:20

... develop robust methods of DNA stretching and immobilization that offer both high sensitivity and highthroughput detection of DNA protein interactions Optical and magnetic traps ... stretched and immobilized at physiological pH and salt concentrations. In the present report, we discuss published methods of DNA stretching and immobilization that can be categorized into one of three ... the center of focus of the beam. Hence the particle is trapped at the focal point of the laser, and the relationship between the displacement of the particle from the focal point and the applied...

Ngày tải lên: 22/06/2014, 18:20

... identify the structural difference of duplicated genes, and 3) reveal the expression and evolution of fiber qual-ity differences between upland and sea-island cotton To complete this study, we ... blotting of Exp1 performed on the four species, showed two distinct hybridizing bands after digestion with EcoRI and HindI in Hai7124 and TM-1, and one hybridizing band in G herbaceum and G raimondii ... orthologs of fiber development genes in four cotton species Gene Numbers of exon Length of ORF(bp)/numbers of derived amino acids CAP A 1 , Ath and Atb: 10; D 5 , Dth and Dtb: 9 A 1 , Ath and Atb:...

Ngày tải lên: 11/08/2014, 11:21

... China 3 Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education and Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture, Huazhong ... SJ158 and a novel microsatellite marker that includes a tandem repeat of (TC) n in intron 4. We also determined the allele number and frequencies of the three loci in seven pig breeds and found ... Additionally, the expression patterns of the porcine CA3 gene dur- ing the porcine skeletal muscle development and in different pig breeds were analysed. 2. MATERIALS AND METHODS 2.1. Analysis of CA3 expression...

Ngày tải lên: 14/08/2014, 13:22

... microglia altered the expression of proinflammatory cytokines such as TNF-α and IL-1β and inflammatory mediators including iNOS and NO and the migratory and phagocytic ability of activated microglia, ... Knockdown of Dpysl3 reduces the expression of iNOS expression in activated microglia 66 3.4.5 Knockdown of Dpysl3 reduces production of nitric oxide in activated microglia 67 3.4.6 Knockdown of Dpysl3 ... phagocytosis and proliferation of microglia 68 3.5.1 Knockdown of Dpysl3 alters the structure of F-actin organization 68 3.5.2 Knockdown of Dpysl3 inhibits migration of microglia 69 3.5.3 Knockdown of...

Ngày tải lên: 12/09/2015, 11:24

... hexakisphosphate) is the primary storage form of phosphorus in plants They occupy about 1-3% of the seeds of grains and legumes, and 60-80% of the total amount of plant phosphorus (Nelson, 1967) Phosphorus ... the Lab of Enzyme Technology, Biotechnology R&D Institute (Nguyen Nhat Khoa Tran, 2012) As the continuation of this research, the thesis on "Purification and characterizations of phytase ... concentration was measured by Bradford method (1976) and phytase activity was determined by the method of Heinonen and Lahti (1980) 2.2.1.3 Purification of phytase by cation exchange chromatography ...

Ngày tải lên: 06/10/2015, 13:05

... niger and A flavus The diameter of the zone of inhibition was shown in Table 4 The purified fucoidan could inhibit the growth of A niger and A flavus The MIC value and the zone of inhibition of ... buffers of Tris-HCl, phosphate, acetate and four given NaCl concentrations The effects of buffers and concentrations of NaCl were depicted in Figure 1 Figure 1 The effects of buffers (a) and concentrations ... R B., Padalia R B., and Shinde K D - Extraction and purification of C-phycocyanin from dry Spirulina powder and evaluating its antioxidant, anticoagulation and prevention of DNA damage activity,...

Ngày tải lên: 13/01/2020, 06:31

... temperature optima of 40°C and retaining an activity of 76% at a temperature range of 20-80°C and followed normal Michael-Menten curve with the kinetic parameter K m and V max of 0.5455mM and 33.927µmol/min ... (Fig 4) A summary of the purification scheme is given in Table4 A 23 fold purification of the phytase was achieved with a recovery of 47.8% and enzyme exhibited an activity of about 66.5U/mg ... optimize and understand the interaction between 4 selected variables i.e galactose, ammonium sulphate, beef extract and yeast extract in an experiments of 30 runs The coded levels of variable and...

Ngày tải lên: 11/03/2020, 11:58

... N., and Panda, T 2003 Purification and biochemical properties of microbial pectinases Process Biochem., 38: 987-996 Hammed, I M S A., and Alyaa, M 2010 Purification and characterization of ... polygalacturonic acid and pectin as substrates Determination of energy of activation and The energy of activation (Ea) was estimated from the slope of Arrhenius plot obtained by plotting the Log of initial ... of distilled water Fill the 3 ml syringe with the sample and slowly charge the focus chamber Then, 6 ml of 0.1 M H3PO4 and 6 ml of 0.1 M NaOH were respectively added to the ventilation hole of...

Ngày tải lên: 05/11/2020, 04:17

... inhibition of CPR could lengthen the durations of the effect of chemicals and consequently increase or decrease the chemical toxicity and affect the metabolism of endogenous compounds since P450 isoforms ... microsomes by primary and secondary DEAE-cellulose column chromatograph with 20.46 µ M/min/mg enzyme specific activities, 54.4% purification yield, and purification of 38-fold The purity of the enzyme ... xenobiotic metabolism and liver microsomal monooxygenase enzyme system, and assist in a better understanding of this system Another aim of this study was to research the in vitro effects of some drugs...

Ngày tải lên: 12/01/2022, 23:29

... protein, a yield of 2.38%, and a purification coefficient of 672-fold Figure 1 exhibits the SDS-PAGE conducted for the purity and molecular weight of the enzyme For the standard proteins and TrxR, Rf ... shown in Figures 5a and 5b for NADPH and DTNB A KM of 12.65 µ M and a V max of 0.513 EU mL−1 were obtained for NADPH, and 0.0828 µ M and 0.079 EU mL −1 were obtained for DTNB V0 and kcat values were ... Research Article Purification and characterization of mitochondrial thioredoxin reductase enzyme from rainbow trout (Oncorhynchus mykiss) liver and investigation of the in vitro effects of some metal...

Ngày tải lên: 12/01/2022, 23:57

... horikoshi,11 The aim of this study was to isolate and identify a new source of thermostable carboxymethylcellulase and investigate the biochemical and catalytic properties of highly purified CMCase ... Decarboxylation of: Growth at: Growth in: (a) Results of Madhaiyan et al 43 , (b) Results of Borriss et al 44 , (c) Results of Roberts et al 45 Trang 4of closely related taxa.14 On the other hand, some ... weight of the purified enzyme was estimated to be about 50 kDa as confirmed by the presence of a single protein band in denatured gel The result of activity staining also showed the active band of...

Ngày tải lên: 13/01/2022, 00:10

... Research Article Investigation of the effects of purification and characterization of turkey (Meleagris gallopavo) liver mitochondrial thioredoxin reductase enzyme and some metal ions on enzyme ... sonicator and so we preferred the freezing and thawing the supernatant was prepared to be used in analyses 2.4 Preparation of 2’,5’-ADP Sepharose 4B affinity column and purification of turkey liver ... metabolism, and possesses a function in such events in the protection of the organism from the damaging effects of peroxide, the production of RNA from DNA, in apoptosis, in cell growth, and in gene...

Ngày tải lên: 13/01/2022, 00:16

... A specific activity of 11.2 U/mg of protein was obtained for the puri-fied enzyme, with a yield of 6.6% and a purification factor of 50 (not shown) The SDS-PAGE analysis of the different fractions ... liver - Purification of membrane-bound BDH from jerboa liver In a typical trial, a total of 5100 mg of protein, corre-sponding to 5.5 units of BDH, was obtained after solubi-lization of mitoplast ... CL-6B, and immu-noaffinity columns Table 1 summarizes the results of the purification process A specific activity of 0.030 U/mg of protein was obtained for the purified enzyme, with a yield of 0.50%...

Ngày tải lên: 02/11/2022, 11:35

... immunohistochemistry also in rodents [22] Expression of ADH3 in the adult rodent and human brain Overviews of the expression patterns of the three classes of ADHs in the adult rodent brain are shown ... magnification of ADH3 expression in rat brain Many but not all neurons in cortex were ADH3 positive and all cerebellar Purkinje cells were strongly positive Expression in the granular layer of rat and ... metabolism, Ó FEBS 2003 ADH expression patterns in mammalian brains (Eur J Biochem 270) 1321 Fig Expression patterns of the different classes of ADH in the brains of rat and mouse at three different...

Ngày tải lên: 21/02/2014, 00:20

... classes of milk (See both USDA NASS release dates and formulas at the back of Primer) Of significance (to both hedgers and traders) is the price relationship of the major milk component of cheese ... lowering of price supports by the government, along with increased demand, mainly for cheese and cheese products, have brought about a price equilibrium between the supply of milk and the uses of milk ... into futures and options markets The Milk futures and options contracts trade each month, can be offset at any time, or can be held through contract expiration and be automatically offset at the...

Ngày tải lên: 21/12/2013, 01:20