enzyme activities of nonstructural protein 3

... 2H, –CH ), 2.31 (s, 3H, CH ) 13 C NMR δ ppm (CDCl ): 169.1, 153.3, 142.7, 141.3, 140.2, 136.3, 136.3, 131.2, 130.9, 130.7, 129.2, 128.4, 127.9, 123.4, 122.9, 119.9, 109.7, 45.6, 13.9 2-(2-(4-Chlorophenyl)-1H ... 165.6, 162.9, 160.6, 153.9, 143.2, 136.6, 135.6, 134.5, 132.2, 131.3, 130.9, 128.9, 128.4, 127.7, 123.7, 123.5, 122.5, 121.8, 120.3, 115.7, 110.2, 70.4, 39.9, 36.3 2-((2-(4-(Benzyloxy)phenyl)-1H ... 13 C NMR δ ppm (CDCl ): 165.5, 162.8, 160.4, 153.7, 142.9, 136.4, 135.4, 134.9, 134.9, 132.2, 131.9, 131.1, 129.6, 128.7, 128.2, 127.5, 127.4, 123.4, 123.3, 121.6, 120.1, 115.4, 109.9, 70.1, 39.6,

Ngày tải lên: 12/01/2022, 23:27

... quantitation of microgram quantities of protein utilizing the principle of protein–dye binding Anal Biochem 72, 248–254 34 Laemmli UK (1970) Cleavage of structural proteins during the assembly of the ... mass of rmDNase1l3 ( 34 kDa) from the zymograms of DNase1 and DNase1l3 By comparing the properties of rmDNase1 and rmDNase1l3 in the hydrolysis of pDNA and chromatin with those of serum collected ... against mDNase1l3, using cell extracts of NIH-3T3 fibroblasts transiently transfected with mDNase1 or mDNase1l3 as a control the corresponding NIH-3T3 cell extract and that mDNase1l3 purified from

Ngày tải lên: 30/03/2014, 02:20

... buffer (pH 3.0), and 250 µM H2O2 The extinction coefficient of veratryl aldehyde (oxidized veratryl alcohol) at 310 nm is 9,300 M-1cm-1 One unit of enzyme activity represents the oxidation of veratryl ... Springer, Berlin, pp 249-273 19 Davis TN, Urdea MS, Masiarz FR, Thorner J (1986) Isolation of the yeast calmodulin gene: Calmodulin is an essential protein Cell 47:423-431 Gold MH, Kuwahara M, Chiu ... and characterization of an extracellular H2O2-requiring diarylpropane oxygenase from the white rot basidiomycete, Phanerochaete chrysosporium Arch Biochem Biophys 234:353-362 Heinzkill M, Messner

Ngày tải lên: 20/06/2014, 20:20

... RP2 03 BR DEN3 290 02 BR D3BR GO5 03 D3 BR RP AAF 2007 BR D3BR RP1 03 PY D3PY AS10 03 BR D3BR IG10 03 BR D3BR SL3 02 PY D3PY PJ4 03 PY D3PY PJ5 03 PY D3PY PJ6 03 BR D3BR PV1 03 BR D3BR PV3 03 BR ... D91 393 91 Thail D92 431 92 ThD3 0396 88 Mal LN7029 94 Mal LN7933 94 ThD3 0213 88 Thail D91 538 91 ThD3 87 Thail 87 1384 87 ThD3 0220 85 ThD3 0065 86 ThD3 0134 83 ThD3 0402 85 ThD3 0183 85 ThD3 ... 423 3 T C 588 3 A G II 633 3 C T 672 3 C T 784 1 C T 825 3 C T 1050 3 C T II 1131 3 A G 1170 3 C T 1185 3 G T TM 1314 3 T C 1356 3 G A 1374 3 T A 1473 3 A G Domain I: 1–156 nt (1–52 aa ); 397–573

Ngày tải lên: 12/08/2014, 04:21

... 30 Inductive Non-inductive _ Figure 31 B A Figure 32 A B Figure 33 A B Figure 34 mgb1D WT _ rgs1Dmgb1D magB G183Smgb1D Figure 35 Figure 36 MG010315 MG010105 MG09134 ... Figure 36 MG010315 MG010105 MG09134 Control: Gamma actin MG03982 MG01173 MG01630 Figure 37 A B [...]...Figure 37 A B

Ngày tải lên: 14/09/2015, 09:39

... 31 Opportunities 33 Weakness 34 Threaten 36 CHAPTER 4: COMPARISON 37 CHAPTER 5: SOLUTION 39 Logistics activities of Vinamilk ... warehousing activities 19 3.3 Structure of distribution channels 22 3.4 Ordering and Process to order 27 3.5 Customer care 27 CHAPTER 3: EVALUTATE 31 Strength ... technology application in the process of raising dairy cows with an investment of 300 billion VND and a capacity of 3000 cows /year Logistics activities of Vinamilk Group h Local farmers Farmers

Ngày tải lên: 02/05/2019, 15:36

... ArH’s); 13C-NMR (DMSOd6) δ: 21.4 (­CH3), 106.8, 110.3,113.5 120.3, 125.6, 126.4, 127.5, 132.6, 138.3, 139.6, 142.5, 157.9, 171.7, 177.3, 183.9; MS (m/z): 337 (M+1, 2), 336 (M+, 12), 245 (6), 230 (10), ... pyrazole H-5); 13 C-NMR (DMSO-d6) δ: 21.0 ­(CH3), 21.4 (­ CH3), 109.3, 115.3, 116.8, 120.4, 124.4, 126.6, 127.2, 127.5, 127.8, 129.4, 131.08, 133.9, 133.9, 136.3, 137.7, 139.1, 139.3, 142.5, 148.9, ... 123.2, 124.1, 126.2, 129.3, 134.5, 137.9, 139.2, 140.1, 144.6, 144.9, 145.2, 149.2, 156.9; MS (m/z): 395 (M+1, 1), 394 (M+, 6), 393 (49), 379 (29), 364 (8), 351 (8), 133 (9), 119 (11), 107 (33),

Ngày tải lên: 29/05/2020, 13:09

... carrying out of thesis. Without the intensive assistance of my supervisor the results of this thesis would never have been possible. Further, I would like to thank all professors of Hanoi ... 1,147 MT of both raw materials and finished products. The plastic import value was $1,426mil, equal to four times of export value of $350 in 2005. In 2004, Vietnam exported only 10 percent of total ... actives of Ha Trung plastic joint stock company… 1 Business activities in Ha Trung Company JSC 2 Production Process and Facilities .18 3 Process of work 24 Chapter3 Direction

Ngày tải lên: 16/04/2013, 08:07

... Competitiveness of Tramat Co., Ltd 34 3.1 Company’s action plan for 2010: 34 3.2 Challenges the company faces 35 3.3 Solutions to improving competitiveness of Tramat Co., Ltd 36 3.3.1 ... Achievements of the trading activities from 2007 to 2009 31 2.3 General assessments of competitiveness in trading activities of Tramat Co., Ltd .32 2.3.1 Strengths 32 2.3.2 Weaknesses ... and marketing 36 3.3.2 Solutions to product and warranty 37 3.3.3 Solutions to improving customer services 37 3.3.4 Solutions to fostering and motivation staff 38 CONCLUSION

Ngày tải lên: 22/04/2013, 15:35

... period life Tuesday, November 25th , 2010 THE WORLD OF WORK Lesson3: A student’s work( A4 ) I.Vocabulary: II Reading: *Prediction Guess the amount of time Hoa spends on her work Hoa has periods a ... works hours a week She has about hours of homework every week She works about hours a week before tests Tuesday, November 25th , 2010 THE WORLD OF WORK Lesson3: A student’s work( A4 ) I.Vocabulary: ... WORLD OF WORK Lesson3: A student’s work( A4 ) I.Vocabulary: II Reading: *Check the prediction Hoa has periods a day She goes to school days a week She works 20 hours a week She has about 12hours of

Ngày tải lên: 03/12/2013, 16:11

... 19073-3299 USA The integrative nature of projects and project management can be better understood if we think of the other activities performed while completing a project. For example, some activities ... Management Body of Knowledge (PMBOK Guide) Third Edition 2004 Project Management Institute, Four Campus Boulevard, Newtown Square, PA 190 73- 3299 USA 4 .3. 3 Develop Project ... 73- 3299 USA 85 Chapter 4 − Project Integration Management 3 Project Management Information System The Project Management Information System (PMIS) is a standardized set of

Ngày tải lên: 24/12/2013, 19:15

... of license. Binding to Plasma Proteins Having entered the blood, drugs may bind to the protein molecules that are present in abundance, resulting in the formation of drug-protein complexes. Protein ... (K D approx. 10 –5 –10 –3 M) for plasma proteins than for their specific binding sites (recep- tors). In the range of therapeutically rel- evant concentrations, protein binding of most drugs increases ... cor- responding change in the concentration of bound drug. Protein binding is of great importance, because it is the con- centration of free drug that determines the intensity of the effect. At an identi- cal

Ngày tải lên: 22/01/2014, 00:20

... purpose of saving the cost of a sleeping-berth and of having a chance to classify and clarify the events of the most wonderful week in his life! VII. A Plunge into Wall Street The father of Edward ... front platform of the street-cars, and take them to the postoffice. Thus the boys absolutely knew the growth of their circulation by the weight of their bundles and the number of their front-platform ... city editor of the Brooklyn Eagle had given Edward the assignment of covering the news of the theatres; he was to ascertain "coming attractions" and any other dramatic items of news interest.

Ngày tải lên: 26/01/2014, 17:20

... Class B: O3 X O4 O1: the degree of the participation before the experiment of Class A O2: the degree of the participation at the end of the experiment of Class A O3: the degree of the participation ... goes for demanding challenges, high proficiency, top grades • Goal orientation: The learner is very aware of the goals of learning, or of specific learning activities, and directs his or her efforts ... students of the two former groups are often obstacles towards creating an encouraging learning environment Second, students’ knowledge including both knowledge of English proficiency and knowledge of

Ngày tải lên: 29/01/2014, 14:35

... lines as a result of NF-kappaB trapping by linker-modi- FEBS Journal 276 (2009) 2505–2515 ª 2009 The Authors Journal compilation ª 2009 FEBS A Tadlaoui Hbibi et al 31 32 33 34 35 36 37 fied hairpin ... Tweardy DJ (2003) Identification and characterization of signal transducer and activator of transcription recruitment sites within the epidermal growth factor receptor Cancer Res 63, 3923–3930 Fujitani ... hairpin STAT3 decoy ODN inhibits the transcriptional activity of STAT3 and colocalizes with STAT3 to the cytoplasm of SW480 cells The transcriptional activity of STAT3 after treatment of the cells

Ngày tải lên: 18/02/2014, 08:20

... 1.29 ± 0.24 515 ± 45 398 ± 82 200 0.76 ± 0.05 678 ± 19 893 ± 66 350 0.36 ± 0.03 608 ± 17 1675 ± 144 500 0.14 ± 0.01 369 ± 7 2599 ± 202 1000 1.00 ± 0.18 164 ± 13 163 ± 32 Effect of ionic strength ... 275 (2008) 3783–3792 ª 2008 The Authors Journal compilation ª 2008 FEBS 3783 examples of enzymes with a PTP fold that are capable of hydrolyzing InsP 6 . Initial structural studies of PhyAsr revealed ... [18]. Treatment of PhyAsr with 100 lm H 2 O 2 resulted in 33% of the enzyme being inactivated after 10 min of treatment and 65% being inactivated after 30 min. Incubation of the enzyme for up to

Ngày tải lên: 18/02/2014, 18:20

... Biotechnologie des Plantes, Baˆtiment 630, Universite´ Paris-Sud, F-91405 Orsay Cedex, France Fax: +33 1 69153423 Tel +33 1 69153338 E-mail: stephane.lemaire@u-psud.fr (Received 3 October 2006, revised 25 ... time of inactivation (min) C153S A 4 -GAPDH half time of inactivation (min) A2B2-GAPDH half time of inactivation (min) A 8 B 8 -GAPDH half time of inactivation (min) 0 5 3 3 0 4 6 3 2 1 3 3 35600 ... 20 40 60 80 100 36324.5 36633.4 36326.0 Mass (m/z) 0 20 40 60 80 100 Fig 9 MALDI-TOF mass spectrometry indicates that C153S A 4 -GAPDH undergoes glutathionylation in the presence of H 2 O 2 and

Ngày tải lên: 19/02/2014, 05:20

... accepted 11 November 2003) Eur. J. Biochem. 271, 173–185 (2004) Ó FEBS 2003 doi:10.1046/j.1432-1033.2003.03917.x important is the modeling of the folded state, where the errors of computing pK a s ... significantly on it [33–36] The success of the modeling of the pH dependent free energy of folding critically depends of the accuracy of the calculated pKas of the ionizable ... dynamic runs [33] or some assumptions of the organization of the amino acids in unfolded state [34,36] or even an experimental determination of the pKas in model compounds [35,73] Our goal

Ngày tải lên: 19/02/2014, 12:20