... identify the presence of viral RNA associated with the highly pathogenic Eurasian origin H5 outbreak virus in a single synanthropic bird associated with a poultry farm in northwest Iowa This ... poultry houses, but only on infected farms In addition, several fresh wild bird carcasses found associated with the poultry operations were also sampled Samples were collected in accordance with ... environment and these materials could subsequently be brought into contact with poultry during the movement of poultry workers or associated equipment and supplies Another potential mechanism leading
Ngày tải lên: 19/03/2023, 15:37
... from surface-applied or incorporated poultry litter with or without acidified biochars (control treatment subtracted) Legend for Table 2: PL refers to poultry litter; PC and PH refer to biochar ... readings of poultry litter alone or in combination with soil, with or without acidified biochars PL + PC 7.26 c 6.47 b PL + PH 7.39 b 6.73 ab Legend for Table 3: PL refers to poultry litter; PC and ... prevented us from using it Trang 8Table 4 Inorganic N leached with 0.01 M CaCl2 from soil (64.5 g, s) and poultry litter (PL, 2.1 g) with or without acidified biochars (PC, PH, 2.1 g) surface-applied
Ngày tải lên: 04/12/2022, 16:07
Tài liệu Viruses associated with human cancer docx
... is also associated with oral and other anogenital malignancies, however, it is most commonly associated with cervical cancer; in fact, over 99% of all cervical cancers are associated with high-risk ... often is the virus associated with the tumor?) 2 Consistency (has the association been observed repeatedly?) 3 Specificity of association (is the virus uniquely associated with the tumor?) 4 ... tumor viruses Although retroviruses have been associated with many animal tumors, to date, only one human retrovirus, HTLV-1, has been associated with human cancers The biology of HTLV-1 will
Ngày tải lên: 15/02/2014, 05:20
Tài liệu Báo cáo khoa học: The splicing factor ASF/SF2 is associated with TIA-1-related/ TIA-1-containing ribonucleoproteic complexes and contributes to post-transcriptional repression of gene expression doc
... and TIAR Nuclei were stained with 4¢,6-diamidino-2-phenylindole (blue). Trang 6is also associated with TIA-1 (Fig 3B), thereby indicat-ing that ASF⁄ SF2 can interact with both TIA proteins Western ... migrate into SGs in cells trea-ted with arsenite in combination with puromycin (data not shown) Furthermore, ASF⁄ SF2 co-localized exclu-sively with TIAR and not with Dcp1-positive Pbs [21], thereby ... cells were transfected with the DNA constructs encoding the HA-tagged interact-ing candidates and were treated with arsenite (1 m M for 30 min) Cells were fixed and stained with mouse anti-HA and
Ngày tải lên: 16/02/2014, 15:20
Tài liệu Báo cáo khoa học: Identification of a novel matrix protein contained in a protein aggregate associated with collagen in fish otoliths pdf
... soaked with 20 mm blocking with 5% fat-free dried milk in NaCl⁄ Tris (50 mm were incubated with the antiserum at a 1 : 1000 dilution overnight Each immunoreacted membrane was then incu-bated with ... anti-rOMM-64 affinity beads were allowed to react with saccular and otolith matrix extracts After incubation with the oto-lith matrix extract and stringent washing with acidic glycine, the beads were found ... h and then with anti-rOMM-64 serum immunoreaction with secondary antibody (1 : 2000, peroxi-dase-conjugated anti-rabbit IgG, Bio-Rad) for 2 h, the sec-tions were washed three times with NaCl⁄
Ngày tải lên: 18/02/2014, 17:20
Tài liệu Báo cáo khoa học: Down-regulation of heme oxygenase-2 is associated with the increased expression of heme oxygenase-1 in human cell lines docx
... cells with HO-2 siRNA induced the expres-sion of HO-1 mRNA and protein without affecting the concentration of GAPDH mRNA and b-actin (Fig 4A,B) Likewise, the selective reduction of HO-2 mRNA with ... Consistent with HO-1 mRNA expression, expression of HO-1 protein was also induced by the treatment with HO-2 siRNA in HepG2 cells These results indicate that the down-regulation of HO-2 expression is associated ... transuntrans-fected with siGAPDH or siHO-1, the heme content was twofold higher after treatment with hemin (Fig 9A), whereas the heme content remained unchanged in HepG2 cells treated with hemin (Fig
Ngày tải lên: 19/02/2014, 05:20
Tài liệu Báo cáo khoa học: Antioxidant defences in cybrids harboring mtDNA mutations associated with Leber’s hereditary optic neuropathy docx
... from 37% in the cells with the 14484⁄ ND6 mutation to 68% in those with the 11778⁄ ND4 mutation, com-pared with the controls Comparison of the results reported in Fig 7 with the data in Fig 4B ... complex I activity [3,6] and oxygen consump-tion (present data) and are associated with severe neuropathology [38], are associated with the highest mitochondrial ROS production [14] and the least effi-cient ... After 24 h, the culture medium was replaced with fresh complete DMEM supple-mented with 25 mm glucose (glu-DMEM) or with glucose-free DMEM supplemented with 5 mm galactose, 5 mm sodium pyruvate
Ngày tải lên: 19/02/2014, 16:20
Tài liệu Báo cáo khoa học: The distinct nucleotide binding states of the transporter associated with antigen processing (TAP) are regulated by the nonhomologous C-terminal tails of TAP1 and TAP2 ppt
... antigen presentation; transporter associated with antigen processing; endoplasmic reticulum; peptide trans-port; nucleotide binding domains The transporter associated with antigen processing (TAP) ... histocompatibility complex; NBD, nucleotide binding domain; TAP,transporter associated with antigen processing; tapasin,TAP-associated glycoprotein; TMD,transmembrane domain. *Present address: Max ... (Sigma)] Immunoprecipitations with anti-rat TAP2 (116/ 5) were performed as described previously [23] Immuno-precipitates were washed with NaCl/Pi,1% Triton X-100 and eluted with 10 mM Tris/HCl pH 8.8
Ngày tải lên: 20/02/2014, 02:21
Tài liệu The Economics Associated with Outdoor Recreation, Natural Resources Conservation and Historic Preservation in the United States pdf
... Trang 1The Economics Associated with Outdoor Recreation, Natural Resources Conservation and Historic Preservation in the ... to larger levels • The combined spending effect of hunting, fishing and wildlife watching associated with National Forest Service land totaled $9.5 billion in annual retail sales, supported 189,400 ... properties elsewhere Cross-Cutting Department of Interior Activities • Overall, in 2010 activities associated with DOI lands provided more than 2.2 million jobs for Americans, which generated $377 billion
Ngày tải lên: 21/02/2014, 01:20
Báo cáo khoa học: Muramyl-dipeptide-induced mitochondrial proton leak in macrophages is associated with upregulation of uncoupling protein 2 and the production of reactive oxygen and reactive nitrogen species docx
... treated with MDP presented a significant decrease in respiratory control ratio, an effect that was absent following treatment with a non-toxic analogue such as murabutide Stimulation of cells with ... respectively, with UCP1, and 73% iden-tity with each other [1], whereas UCP4 and UCP5 (also referred to as brain mitochondrial carrier protein 1, BMCP1) have much lower sequence identity with UCP1 ... However, MDP adminis-tration into different hosts was associated with serious toxicity Therefore, attempts have been made to gener-ate analogues with desirable properties and reduced toxicities One
Ngày tải lên: 05/03/2014, 23:20
Indoor Air Pollution Associated with Household Fuel Use in India: An exposure assessment and modeling exercise in rural districts of Andhra Pradesh, India doc
... USEPA United States Environmental Protection Agency Trang 14Department of Environmental Health Sciences, School of Public Health, University of California, Berkeley Department of Environmental Health ... pollutants associated with combustionof solid fuels in households of developing coun-tries are now recognized as a major source of health risks to the exposed populations Use of open fires with simple ... correlated with fuel quantity,while only living area concentrations were corre-lated with the number of rooms and windows Nei-ther kitchen nor living room concentrations wassignificantly correlated with
Ngày tải lên: 06/03/2014, 16:20
Traffic-related air pollution associated with prevalence of asthma and COPD/chronic bronchitis. A cross-sectional study in Southern Sweden pdf
... emission database for NOx Results: Living within 100 m of a road with >10 cars/minute (compared with having no heavy road within this distance) was associated with prevalence of asthma diagnosis ... were not associated with self-reported traffic exposure (tables 5, 7) Association with traffic intensity on heaviest road within 100 m Asthma diagnosis and asthma symptoms were associated with traffic ... symptoms Self-reported traffic exposure was associated with asthma diagnosis and COPD diagnosis, and with asthma symptoms Annual average NOx was associated with COPD diagnosis and symptoms of asthma
Ngày tải lên: 06/03/2014, 19:20
Báo cáo khoa học: Functional analysis of mutations in UDP-galactose-4epimerase (GALE) associated with galactosemia in Korean patients using mammalian GALE-null cells pdf
... acids that have been characterized, none of the mutations with mild defects are associated with these conserved amino acid residues, with the exception of N34S By contrast, all seven GALE mutants ... the protein with myc antibody When increasing amounts of lysate from cells transfected only with FLAG–GALE were immunoprecipitated, no GALE bands were detected on western blots probed with anti-body ... 293T cells grown on coverslips were transfected with myc– GALE or FLAG–GALE, fixed with 4% paraformaldehyde in NaCl⁄ Pifor 20 min, and permeabilized with 1% Nonidet P-40⁄ 10 mm glycine for 5 min
Ngày tải lên: 07/03/2014, 00:20
Báo cáo khoa học: Endogenous tetrahydroisoquinolines associated with Parkinson’s disease mimic the feedback inhibition of tyrosine hydroxylase by catecholamines doc
... with the essential pterin cofactor (Fig 2B); hTH inhibition by NMNorsal was noncompetitive with respect to tyrosine (data not shown) We compared the inhibitory effects of NMNorsal and NMSal with ... recombinant human TH, we show here that endogenous TIQs associated with PD mimic the mech-anisms of catecholamine feedback inhibition: TIQs both compete with the tetrahydropterin for access to the active ... obtained with MPTP However, nei-ther NMTIQ nor MPTP formed electrostatic interac-tions with the surrounding TH residues, resulting in a low binding affinity NMTIQ is therefore unlikely to compete with
Ngày tải lên: 07/03/2014, 05:20
Báo cáo khoa học: Cellular refractoriness to the heat-stable enterotoxin peptide is associated with alterations in levels of the differentially glycosylated forms of guanylyl cyclase C pdf
... 20-day-old Caco2 cells were washed with serum-free DMEM/F12 and incubated with or without 10)7MSTh in DMEM/F12 for 9 h Monolayers were then washed and re-stimulated with fresh STh (10)7M) in the presence ... desensitized cells This is concomitant with a 50% reduction in ST-binding activity in desensitized cells Desensitization was correlated with a loss of the plasma membrane-associated, hyperglycosylated ... Cells were then incubated overnight with 5 lgÆmL)1GC-C:4D7, an antibody raised to the protein kinase-like domain of GC-C [32] or with GC-C:4D7 antibody preadsorbed with a fusion protein of the kinase-like
Ngày tải lên: 08/03/2014, 08:20
Visitor Profiles, Economic Impacts and Recreational Aesthetic Values Associated with Eight Priority Florida Springs Located in the St. Johns River Water Management District pot
... assistance with the project’s report editing tasks along with Chris Ware, Environmental Scientist, SJRWMD Carol Miller, Administrative Assistant, SJRWMD, is to be recognized for her help with administrative ... Springs Table 2.5 16 Estimation of the Direct Economic Impact of Visitors Associated with Silver Glen Springs, Marion County, Florida 3rd Quarter (July-Sept), 2003 Table 2.6 ... Publication SJ2004-SP35 Visitor Profiles, Economic Impacts and Recreational Aesthetic Values Associated with Eight Priority Florida Springs Located in the St Johns River Water Management District
Ngày tải lên: 08/03/2014, 08:20
Báo cáo khoa học: N-Methylation in polylegionaminic acid is associated with the phase-variable epitope of Legionella pneumophila serogroup 1 lipopolysaccharide pptx
... containing LPS species with different OPS chain-lengths (Fig 4B) A ladder-like pattern was observed with differences of up to 10 sugar residues within each fraction and with overlap-ping about ... NOESY with mixing time 300 ms), and H-detected 1H,13C NMR (HMQC and HMBC) spectra were recorded with a Bruker Avance DRX-600 spectrometer.13C NMR and DEPT-135 NMR spectra were recorded with a ... °C for neutral sugars [32] or with 10MHCl for 30 min at 80 °C for amino sugars [33] Following hydrolysis, sugars were dried, reduced with NaBH4, and acetylated with acetic anhydride in pyridine
Ngày tải lên: 08/03/2014, 10:20
SPECIAL STUDY: INJURIES AND DEATHS ASSOCIATED WITH CHILDREN’S PLAYGROUND EQUIPMENT doc
... injuriesresulted in an estimate of 156,040 injuries associated with public equipment, 46,930injuries associated with home equipment, and 2,880 injuries associated with homemadeequipment.The remainder of ... of the injuries associated with swings involved falls, about 69 percent of the injuries associated withslides involved falls, and about 59 percent of the injuries associated with other types ... School-age children ages 5-9 years wereassociated with the largest portion of injuries, 56 percent Older school-age children ages10-14 years were associated with about 14 percent of the injuries
Ngày tải lên: 08/03/2014, 13:20
Rapid intercontinental air pollution transport associated with a meteorological bomb docx
... Germany 3Institute of Environmental Physics, University of Bremen, Germany 4Institute of Environmental Physics, Heidelberg University, Germany 5Cooperative Institute for Research in Environmental Sciences ... Martin et al 2002) The accuracy of tropospheric NO2columns from GOME is mainly limited by problems associated with cloud con-tamination, errors introduced by the correction of the strato-spheric contribution, ... analyses with a resolution of 1◦ Continental outlines are shown as thick grey lines, and synoptic systems are labeled, as described in the text, with bold white letters northeast of their center with
Ngày tải lên: 15/03/2014, 20:20
Báo cáo khoa học: Aberrant interchain disulfide bridge of tissue-nonspecific alkaline phosphatase with an Arg433 fi Cys substitution associated with severe hypophosphatasia pdf
... Trang 1alkaline phosphatase with an Arg433 fi Cys substitution associated with severe hypophosphatasia Makiko Nasu1, Masahiro Ito2, Yoko Ishida2, Natsuko ... the molecular basis for the lethal hypophosphatasia associated with TNS-ALP(R433C) Trang 3TNSALP(R433H) The cells were metabolically labeledwith [35S]methionine⁄ cysteine for 3 h and subjected ... plasmid encoding TNS-ALP(R433C) were cultured with 0.5 lgÆmL)1doxycycline for 24 h After fixation, the cells were reacted with anti-TNSALP and then with anti-(rabbit IgG)–rhodamine (B) The established
Ngày tải lên: 16/03/2014, 12:20