endothelial function and oxidative stress in cardiovascular diseases

... paraffin blocks and stained with haematoxylin and eosin Organ and blood samples were examined for the presence of F tularensis by culture and the mouse inoculation test Agglutination antibody ... dying on day 9 post-inoculation The remaining three hares were killed on day 35 post-inoculation There were no gross and microscopic pathological findings in the surviving hares, and organs collected ... found in total cholesterol, triglycerides and creatine kinase Figures 1 and 2 demonstrate differences in the develop-ment profile of total protein and albumin As shown, the levels of total protein

Ngày tải lên: 01/11/2022, 08:52

... to neu-rodegenerative diseases in humans81 However, the link between increased brain oxidative stress and any brain dysfunction in the SE offspring remains to be elucidated In conclusion, maternal ... inflammation and hypoxia, which were lower in the SE mothers, however higher in mature SE offspring Increased brain oxidative stress and chronic inflammation are evident in certain neurodegenerative diseases, ... HIF-1α in the SE offspring Considering the protective effect of HIF-1α , its increase in the brains of SE offspring may be an adaptation to protect against increased oxidative stress in the brain

Ngày tải lên: 04/12/2022, 14:50

... Synaptojanin-1 is an inositol phosphatase involved in synaptic vesicle individuals with DS display an increase in the number and size of astrocytes and a high Trang 22in humans in order to understand ... Treacher Collins syndrome of cranial NCC origin in which individuals develop hypoplasia of facial bones including the maxilla and mandible, and alterations of the external and inner ears, leading to ... Midgestation in the PA1 59 5.3 EGCG and Harmine Ameliorate Proliferation and Migration Deficits in Ts65Dn PA1 and NT Tissues In Vitro 60 5.4 EGCG and Harmine Ater Proliferation and Migration in Ts1Rhr

Ngày tải lên: 24/08/2014, 11:47

... angiopoietin family is composed of four ligands (Angiopoietin1, Angiopoietin2 and Angiopoietin3/4) and two corresponding tyrosine kinase receptors (Tie1 and Tie2) Ang3 and Ang4 are orthologs found in ... Ang1, Ang2 and Tie2 Hypoxia induces VEGF expression in ECs and pervascular cells and regulates EC functions via autocrine and paracrine VEGF signaling It is very interesting to note that intracellular ... many basement membrane proteins produces many antiangiogenetic fragments including angiostatin, endostatin, kininostatin, endorepellin ,restin, tumstatin and vastatin (Chen et al 2006, Jimenez

Ngày tải lên: 24/08/2014, 11:59

... to investigate the protective mechanisms of GABA and Pu-erh tea leaf extract on KA-induced injury in neuronal cellsin vivo and in vitro Methods Materials GABA and kainic acid (KA) were obtained ... Coronal paraffin sections were prepared with Hematoxylin and Eosin (H&E) stain-ing for cells damage and TUNEL stainstain-ing to assess apop-tosis study After fixation for 1 h, mice brain sections ... Figure 6 In vitro and in vivo effect of PETL extract and GABA on the KA-induced oxidative stress KA-induced lipid peroxidation of PC12 cells and brain neuron tissue of FVB mice were determined by

Ngày tải lên: 10/08/2014, 10:20

... Pulmonary Medicine, Helsinki University Central Hospital, Helsinki, Finland, 3 Clinical Physiology, Helsinki University Central Hospital, Helsinki, Finland and 4 Department of Medicine, University ... tables and fig-ures and participated in the writing process TH participated in the recruitment and interview of the sub-jects and their characterization HI has participated in the recruitment and interview ... lung function parameters than in non-smokers This is also in line with increased levels of lactoferrin in cigarette smokers, since lactoferrin is located in neutrophils In agreement with the negative

Ngày tải lên: 12/08/2014, 16:20

... noticed adhering to endothelial wall 72 hrs (d) and 168 hrs (e) after PHx, depicting features similar to those seen in (c) (f) Ten days after PHx an intact endothelial lining (arrow) and fenestrae ... adhered to the endothelial lining In addition, the endothelial lining became disrupted as represented by the occurrence of gaps and microvilli, which were facing directly toward the sinusoidal lumen ... before and after shear stress Immunofluoresence of LECs before and after shear stress LECs were reacted with anti VE-cadherin and VEGFR-2 alone and in conjunction before and after exposure to laminar

Ngày tải lên: 13/08/2014, 13:20

... data sug-gest that in the Doberman pinchers copper accumulates in time and, finally, will have its negative effect on copper metabolism and induce oxidative stress Oxidative stress has been ascribed ... with copper incorpo-rated during biosynthesis Metallothionein 1A (MT1A) is a small intracellular protein capable of chelating several metal ions, including copper It contains many cysteine residues, ... values in the N-CASH and CASH group In contrast, many of these (including ATP7A, ATP7B, ceruloplasmin, and metallothionein) were significantly reduced in the DH group Measurements on defences against

Ngày tải lên: 13/08/2014, 13:20

... stress and inflammation are related In the initial inflammation, immune cells migrate to inflamma-tory sites and release various proinflammainflamma-tory cytokines that function in a coordinative ... determined according to Sinha [58] Histological examination The lungs were removed, inflated at 25 cm pressure with PBS then fixed, embedded in paraffin, sectioned and stained with hematoxylin and ... cancer and can cause pulmonary fibrosis as a complication, has been used in research to induce pulmonary fibrosis in mice and rats and produce an oxidative injury resulting in DNA damage and the

Ngày tải lên: 13/08/2014, 14:20

... (baseline) and every 10 minutes during the observation period In vitro measurements Determination by electron paramagnetic resonance (EPR) NO spin trapping Aorta and heart samples were incubated ... enzymes, HO-1 and HO-2 Indeed, Maineset al [30] reported increased levels of HO-1 in I/R injuries; moreover, HO-1 was found to improve resistance to oxidative stress [32] and modulate inflammatory ... NaHS on inflammatory pathway signaling (a, d) Western blots revealing NF-kB expression in the aorta (a) and in the heart (d) (b, e) Western blots revealing P-I B expression in aorta (b) and in heart

Ngày tải lên: 13/08/2014, 21:21

... [30] For instance, inhibition of mitochondrial complex I in PD and ALS, complexes II and III in HD, and complexes II and IV in AD stimulate disorganized oxidative phosphorylation and result in apoptosis ... amplifying H 2 S level induces several molecular changes in neurodegenerative diseases by the increasing and decreasing the enzymes including CBS, CSE, and 3-3MST H 2S also exerts its antioxidant function ... altered function under oxidative stress Oxidative stress-induced mutations in mtDNA have harmful effects on mitochondrial function over time mtDNA mutations result in abnormalities in the oxidative

Ngày tải lên: 15/01/2020, 12:10

... tolerance in yeast [26] Loss of function analyses of Arabidopsis AtHSFA4c and rice OsHSFA4d has indicated additional HSFA4 functions These functions include the sensing of moderate oxidative stress and ... as explained with detail in the Methods section, we performed careful controls to insure equal loading of total protein in all the 1D and 2D-western analyses included in this report The single ... HaHSFA4a protein in the 35S:A4a and 355:A9/A4a seedlings 1D-western analyses using anti-hemaglutinin antibodies Additional file 5: Examples of total protein loading controls for the protein samples

Ngày tải lên: 27/05/2020, 01:25

... [45,46], and the causal link between salinity and oxidative stress signalling and reactive species detoxification is becoming evident [47] At the same time, attempts to link plants salinity tolerance ... term salinity stress tolerance under field conditions Other mechanisms contributing to restricted Na+ accumulation in the shoot include reduced Na+ loading into the xylem [6,22] and increased ... ionic, osmotic and oxidative stress components towards overall salinity stress tolerance in this species, both at the whole-plant and cellular levels In addition, transcriptional changes in the gene

Ngày tải lên: 27/05/2020, 01:49

... multiple lysine residues and enhanced its DNA binding activity, leading to cell cycle arrest; and 3) induced oxidative stress by suppressing the thioredoxin system, consequently inhibiting the activation ... examined the antineoplastic properties of PA-2 in TNBC in vitro and in vivo PA-2 was much more potent than aspirin in inhibiting the growth of TNBC cells and strongly suppressed TNBC growth in ... con-sistent with previous findings [20] We also determined cell proliferation and apoptosis in MDA-MB-231 xenografts in the treatment study (Figure 2A) using Ki-67 staining and TUNEL assay, respectively

Ngày tải lên: 05/11/2020, 01:26

... 3confirmed that the control group mainly engaged indesk work not often involving copy printing and had never engaged in handling work The toner-handling area and the area where the control group ... study, toner-handling work was not associated with the deterioration of respiratory function and an increase in biomarker values for inflammation, allergy, and oxidative stress This finding suggests ... and oxidative stress, such as those for C-reactive protein (CRP), immunoglobulin E (Ig E), interleukin (IL)-4, IL-6, IL-8, and interferon-gamma (IFN-γ) in serum, and 8-hydroxy-2′-deoxyguanosine

Ngày tải lên: 06/05/2021, 15:31

... expression in the process of OA implies an imbalance in regulatory processes governing oxidative stress and inflammation, potentially linking changes in metabolism to osteoarthritic changes In our ... 5LXR, RXR, and PPAR agonism promote changes in genesinvolved in ECM homeostasis and chondrocyte metabolism Genes induced in the microarray were primarily involved in metabolic processes or in extracellular ... treatments was Txnip, which encodes the Thioredoxin interacting protein Txnip inhibits Thioredoxin and contributes to ER stress, inflammasome activation, and the accu-mulation of reactive oxygen species

Ngày tải lên: 04/12/2022, 15:53

... epimerase 1 and condensation domain 1 (E1 and C1) occur between nucleotides 1485 and 3783 The adenylation domains 2 and 4 (A2 and A4) occur between nucleotides 4326 and 5505 and 10 710 and 11 919, ... 0.001) and increased sensitivity to oxidative stress (P¼ 0.002) in culture and during neutrophil-mediated phagocytosis In addition, the mutant exhibited altered conidial surface morphology and hydrophilicity, ... protein), and a typical final protein profile is shown in Fig 3D A dominant protein band was obvious at approximately 220 kDa (indicated by arrow) that was associated with an immu-noreactive band

Ngày tải lên: 30/03/2014, 10:20

... apoptosis In fact, ROS-induced oxidative stress and cell death play important roles in the efficacy of many antineoplastic agents [13,14] To investigate whether oxidative stress is involved in the ... LOX inhibitors, including aesculetin (a nonselective LOX inhibitor), MK886 (an inhibitor of the 5-LOX-activating protein), zileuton (a 5-LOX inhibitor), baicalein (a 12/15-LOX inhibitor), and ... 2-), and hydroxyl radical (OH·2-), are generated in cells by Figure 1 NSC-741909-induced MKP1 and MKP7 clustering, and ROS production in sensitive cell lines (A, B), Clustering of MKP1 (A) and

Ngày tải lên: 18/06/2014, 16:20

... subunit is present in mouse brain in cortex, in hippocampal neurons, and in Purkinje cells [73] Neuronal cells respond to toxic insults including ischemia [73], zinc overload [72], and nerve growth ... global inhibition of src-family tyrosine kinases or inhibition of phosphatidylinositol-3 kinase (PI3K) attenuates ROS production These findings suggest that these kinases are involved in upstream ... within microglia and astrocytes including the nicotinamide adenine dinucleotide phosphate (NADPH) oxidase, mitochondria respiratory chain, xanthine oxidase, microsomal enzymes, cycloxygenase and

Ngày tải lên: 19/06/2014, 22:20

... kingdoms of life (Landry et al, 1982; Vierling, 1991; Parsell and Lindquist, 1994), provides a link between conditioning treatments that induce heat shock protein production and those increasing ... high and low temperature stress and wing size was examined within and among four Drosophila melanogaster populations from temperate (Denmark and Italy) and subtropical areas (Canary Islands and ... avoiding overcrowding Mass populations were obtained by pooling lines of each population in cages with overlapping generations Flies were maintained on a medium of yeast, sugar, cornmeal and agar

Ngày tải lên: 09/08/2014, 18:22