embryonic stem cells biology definition

... transcripts in the undifferentiation of human embryonic stem cells; among the miRNAs that are highly expressed in undifferentiated embryonic stem cells, the miR-520 cluster may be closely involved ... human [16,17] and mouse embryonic stem cells [18-20] The unique patterns of miRNA expression in embryonic stem cells suggest they are involved in maintaining "stemness" Identifying mRNAs that ... miRNA upregulated in hES cells, 31 upregulated in EB cells, and 35 upregulated in adult cells (Figure 2) The 20 miRNAs most highly expressed in hES cells, EB, and adult cells respectively were

Ngày tải lên: 18/06/2014, 15:20

... murine embry-onic stem cells Biol Reprod 1997, 57(3):561-8. 26. Bonde S, Zavazava N: Immunogenicity and engraftment of mouse embryonic stem cells in allogeneic recipients Stem Cells 2006, 24(10):2192-201. ... Immunosuppression by embry-onic stem cells Stem Cells 2008, 26(1):89-98. 31. Fabricius D, Bonde S, Zavazava N: Induction of stable mixed chi-merism by embryonic stem cells requires functional Fas/ ... pro-teins in human embryonic stem cells Proc Natl Acad Sci USA 2002, 99(15):9864-9. 23. Li L, Baroja ML, Majumdar A, Chadwick K, Rouleau A, Gallacher L, et al.: Human embryonic stem cells possess

Ngày tải lên: 18/06/2014, 15:20

... that employ adult stem cells (such as blood-forming hematopoietic stem cells and cartilage-forming cells) A potential advantage of using adult stem cells is that the patient's own cells could be ... and characterization of human embryonic stem cells Stem Cells Dev 2004, 13, 325-336 18 Drukker M, Benvenisty N The immunogenicity of human embryonic stem-derived cells Trends Biotechnol 2004, ... from mouse embryonic stem cells Nat Biotechnol 2000, 18, 675-679 41 Levenberg S, Golub JS, Amit M, Itskovitz-Eldor J, Langer R Endothelial cells derived from human embryonic stem cells Proc Natl

Ngày tải lên: 07/08/2014, 18:21

... 61 (Suppl 1), 2-7 16 Chambers I The molecular basis of pluripotency in mouse embryonic stem cells Cloning Stem Cells 2004, 6, 386-391. 17 Cobb MH, Goldsmith EJ How MAP kinases are regulated ... H2O2-induced ROS generation in mouse embryonic stem cells using DCF-DA (10-5M), a fluorescent probe of intracellular H2O2 Production of ROS was detected in the cells exposed to H2O2 10-3 M for a ... mouse embryonic stem cells exposed to H 2 O 2 -induced oxidative stress 253Fig 5 Effect of dihydrotestosterone (DHT) on hydrogen peroxide (H2O2)-induced decrease of DNA synthesis A-D: The cells

Ngày tải lên: 07/08/2014, 20:23

... embryogenesis [9-11] Co-culture of ES cells with mouse skull-derived stromal cells, such as PA6 cells, or bone marrow-derived cells, such as MS5 cells, efficiently induces the ES cells to become neuron lineages ... the induction of Sox1/N-cadherin/nestin triple positive cells, which represent primitive neuroblasts, from mouse embryonic stem (ES) cells Results: We demonstrated that FGF1, FGF2, and FGF4, ... simple monocul-ture differentiation system for ES cells, these cells often undergo apoptosis on days 3 to 5 Recently, an efficient neural-induction monoculture system with a high sur-* Correspondence:

Ngày tải lên: 10/08/2014, 05:21

... oxygen depletion/reoxygenation con-ditions in mouse embryonic stem cells Methods Mouse embryonic stem cell culture The mouse embryonic stem (MES) cells (CCE-24) were routinely grown on 0.1% gelatin-coated ... the conserved catalytic active site in cultured mouse embryonic stem cells Due to the importance of anti-oxidative stress mechanisms in stem cells, we have performed studies on this isoform in ... differentiation Only cells within the 20th passage were used Anoxia/reoxygenation treatment The anoxic treatment of mouse embryonic stem cells was performed by incubating the cells in an anaerobic

Ngày tải lên: 10/08/2014, 05:21

... referred to as the ‘epigenome’ [19] Abstract Human pluripotent cells such as human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) and their in vitro differentiation models ... underlying genetic variants © 2010 BioMed Central Ltd Epigenomics of human embryonic stem cells and induced pluripotent stem cells: insights into pluripotency and implications for disease Alvaro ... Prevalent in hESCs [66,67] ESC, embryonic stem cell; CTCF, CCCTC-binding factor, insulator associated protein; hESC, human embryonic stem cell; iPSC, induced pluripotent stem cell; H2AZ, histone variant

Ngày tải lên: 11/08/2014, 12:21

... pluripotent ES cells.</p> Abstract Background: The time of locus replication during S-phase is tightly regulated and correlates with chromatin state Embryonic stem (ES) cells have an unusual ... locus replication timing often differs between cell types In embryonic stem (ES) cells, the timing of DNA Published: 17 August 2007 Genome Biology 2007, 8:R169 (doi:10.1186/gb-2007-8-8-r169) Received: ... Trang 1The impact of chromatin modifiers on the timing of locus replication in mouse embryonic stem cells Addresses: * Lymphocyte Development Group, MRC Clinical Sciences Centre, Imperial

Ngày tải lên: 14/08/2014, 08:20

... screen for infection-resistant cells Figure 1 Negative selection screen for infection-resistant cells A gene-trap library in Blm-deficient embryonic stem (ES) cells was co-cultivated with the ... of ES cells by co-cultivation (a) Efficiency of infection by co-cultivation with the viral producer cell line B4-5 AB2.2, wild-type (WT), and NGG5.3 Blm-deficient embryonic stem cells cells ... retroviral infection in a library of insertionally mutated Blm-deficient embryonic stem cells Addresses: * Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing

Ngày tải lên: 14/08/2014, 20:22

... culture of pluripotent stem cells……… 1 1.2 Characteristics of mouse embryonic stem (ES) cells………8 1.3 Application of ES cells……… 10 1.4 Molecular characteristics of ES cells……… …12 Chapter II ... Derivation and culture of pluripotent stem cells Although the era of embryonic stem (ES) cells is considered to begin officially in 1981, when mouse ES cells were first isolated and successfully ... cells, embryonic stem cells, from the mouse embryo Embryonic stem cell lines are derived from the inner cell mass (ICM) of the mouse blastocyst at embryonic day 3.5 (E3.5) These cells were initially

Ngày tải lên: 10/09/2015, 15:51

... relief of symptoms Stem cells represent a promising cell source for cartilage repair and can be derived from two major sources: mesenchymal stem cells (MSCs) and embryonic stem cells (ESCs) (Lee ... which include issues of tumorigenicity and functionality of ESCs 2.2 Human Embryonic Stem Cells (hESCs) Embryonic stem cells (ESCs) are derived from the inner cell mass of the blastocyst-stage ... further advanced with the development of embryonic-like stem cells, also known as induced pluripotent stem cells (iPSCs), as a potential autologous pluripotent stem cell source that can be generated

Ngày tải lên: 11/09/2015, 09:57

... derivation sources, named embryonic stem cells Trang 25(ESCs), embryonic germ cells, fetal stem cells, umbilical cord stem cells, adult stem cells and induced pluripotent stem (iPS) cells (Ariff Bongso, ... 2007) Lymphoid stem cells, Myeloid stem cells Unipotent Retain self-renewal capacity but can only produce one cell type (Sorgner, 2007) Muscle stem cells Apart from the plasticity, stem cells can ... Human embryonic stem cells (hESCs) “Stem cell” is a distinct type of cell that can renew itself and give rise to multiple specialized cell types [National institutes of health (NIH) stem cells

Ngày tải lên: 11/09/2015, 10:00

... underlying human nervous system development (Ben-Nun and Benvenisty, 2006; Dvash et al., 2006) 2.7 Embryonic stem cells and induced pluripotent stem cells Embryonic stem cells (ESC) have two characteristics ... variety of cells that make up the central nervous system including dopaminergic neurons, motor neurons, glial cells, astrocytes, oligodendrocytes, neural crest stem cell cells and retinal cells (Bjorklund ... C PUBLICATIONS 161 ! Trang 9SUMMARY Human embryonic stem cells (hESC) are pluripotent stem cells that have the unique ability to differentiate into cells of the three germ line lineages Hence,

Ngày tải lên: 11/09/2015, 10:14

... factor 1α eGFP enhanced green fluorescent protein ES cells embryonic stem cells FBS fetal bovine serum hES cells human embryonic stem cells HIV human immunodeficiency virus HSV herpes simplex ... specific diseases in the course of ex vivo gene therapy 1.1.1 Human Embryonic Stem Cells Human embryonic stem (hES) cells are cells derived from the inner cell mass of human blastocysts The hES ... vectors for the genetic modification of human embryonic stem cells Although lentiviral vectors are widely used in gene transfer to human embryonic stem cells, there is still a need for vectors and

Ngày tải lên: 11/09/2015, 14:38

... the embryonic stem (ES) cells, embryonic germ (EG) cells and embryonal carcinoma (EC) cells Multipotent stem cells can give rise to cells of certain specialized lineages Many adult stem cells ... differentiation, stem cells in mammals can be grouped into three different types, including totipotent stem cells, pluripotent stem cells and multipotent stem cells Totipotent stem cells can generate ... of pluripotent stem cells Pluripotent stem cells can be isolated from various embryonic sources For instance, EC cells can be derived from teratocarcinomas, while ES cells and EG cells can be

Ngày tải lên: 12/09/2015, 08:19

... potentials: totipotent stem cells, pluripotent stem cells, multipotent stem cells, and unipotent stem cells A totipotent cell can develop into a whole organism Pluripotent cells can give rise to ... application of stem cells which has been impeded by lack of efficient and straightforward ways to obtain the patient-specific stem cells Trang 171.1 Pluripotent stem cells Pluripotent stem cells can ... all of specialized cell types Only one type of cells can be derived from unipotent stem cells Mouse embryonic stem (ES) cells are pluripotent cells with various applications For example, they

Ngày tải lên: 14/09/2015, 14:07

... adult stem cells are the hematopoietic stem cells, mesechymalstem cells and neuronal stem cells Adult stem cells have also been isolated from severalother organs such as the brain (neuronal stem cells), ... blood stem cells give rise to red blood cells,white blood cells and platelets, while skin stem cells give rise to the various types of skincells Some recent reports suggest that adult stem cells ... immuno-privileged because of their embryonic origin.Adult stem cells versus embryonic stem cells The general differences between the characteristics of adult and embryonic stem cells aresummarised in Table

Ngày tải lên: 16/09/2015, 15:55

... marrow (mesenchymal stem cells, hematopoietic stem cells) and (2) embryonic stem and embryonic germ cells derived from discarded human embryos and germ line stem cells Adult stem cells can be derived ... of hES cells is mandatory 1.5 Human Embryonic stem cells As the name suggests, the human embryonic stem cells are derived from the early embryo These cells have the capacity to self renew ... osteogenic cells in bone formation Trang 30CHAPTER 2 MATERIAL & METHODS Trang 312 MATERIAL & METHODS 2.1 Culture and maintenance of Human Embryonic Stem Cells Human embryonic stem cells

Ngày tải lên: 09/10/2015, 11:24

... Human embryonic stem cells 1.1.1 Overview and characteristics of human embryonic stem cells 1.1.2 Regulatory networks and transcription factors in human ES cells 1.1.3 Induced pluripotent stem cells ... Trang 12Chapter 1 Introduction 1.1 Human embryonic stem cells 1.1.1 Overview and characteristics of human embryonic stem cells Embryonic stem (ES) cells are isolated from the inner cell mass ... ‘pluripotent’ stem cells of these cancers (Sperger et al., 2003) Despite their germ cell origin, EC cells share many commonalities with ES cells in various aspects Like ES cells, EC cells proliferate

Ngày tải lên: 16/10/2015, 11:58

... 11B) Embryonic stem cells transfected with FAM-labelled negative control retained a normal mESC morphology 6 days post-transfection, while siOct or siSox transfected cells differentiated into cells ... proposes that embryonic stem (ES) cells for various species do not require external factors such as fibroblast growth factors (FGFs) or wingless homologues (WNTs) to instruct ES cells to become ... Stromal-Derived Inducing Activity (SDIA) co-culture Mouse embryonic stem cells seeded onto PA6; the latter were visible as flat, dark patches of cells beyond the bright, round cluster morphology of

Ngày tải lên: 27/11/2015, 11:25