embryonic stem cells and cardiac progenitors sources of cardiomyocytes

... type of stem cell is the adult stem cells Like ES and EG cells, adult stem cells can self-renew, but unlike ES and EG cells, they give rise to specific sets of mature cell types Adult stem cells ... 1Generation of Hepatocyte-like cells from Mouse Embryonic Stem (ES) and Bone Marrow (BM) Cells YIN YIJUN NATIONAL UNIVERSITY OF SINGAPORE 2004 Trang 2Generation of Hepatocyte-like cells from Mouse Embryonic ... self-renew and to give rise to specialized cells that make up the tissues and organs of the body In general, there are three types of stem cells defined by their origins An embryonic stem (ES)...

Ngày tải lên: 16/09/2015, 12:10

... system to study complex events underlying human nervous system development (Ben-Nun and Benvenisty, 2006; Dvash et al., 2006) 2.7 Embryonic stem cells and induced pluripotent stem cells Embryonic ... specification of the neuroectoderm (Muñoz-Sanjuán and Brivanlou, 2002) Bmp are secreted from roof plate cells in the dorsal neural tube and induce formation of neural crest stem cells and dorsal ... 2.4! SHH and neural development 10! Trang 52.5! SHH and proliferation 14!2.6! SHH in developmental disorders and cancer 15! 2.7! Embryonic stem cells and induced pluripotent stem cells 16!...

Ngày tải lên: 11/09/2015, 10:14

... Introduction 1.1 Human embryonic stem cells 1.1.1 Overview and characteristics of human embryonic stem cells Embryonic stem (ES) cells are isolated from the inner cell mass (ICM) of embryos of blastocyst ... trust, and constant support, which is the driving force behind the successful completion of my research Trang 31.1 Human embryonic stem cells 1.1.1 Overview and characteristics of human embryonic stem ... 42 Materials and methods 2.1 Culture of human ES cell line 2.1.1 Preparation of feeder cells 2.1.2 Maintenance of human ES cells 2.1.3 Preparation of embryoid bodies 2.2 Culture of NCCIT cell...

Ngày tải lên: 16/10/2015, 11:58

... Gokhale PJ, Andrews PW Culture and characterization of human embryonic stem cells Stem Cells Dev 2004, 13, 325-336 18 Drukker M, Benvenisty N The immunogenicity of human embryonic stem-derived cells ... current perspectives of potential applications of stem cells for tissue repair and transplantation medicine are also reviewed Derivation and culture of ES cells In the 1980’s, ES cells were first ... cavity of an expanded blastocyst, ES cells give rise to chimeric mice in which ES cells take part in the development of all types of tissue including the germ line [62] Applications of stem cells...

Ngày tải lên: 07/08/2014, 18:21

... morphology of pluripotent state of ES cells. Localization of endogenous Cx30.3 and Cx43 proteins A comparative analysis of the localization of Cx30.3 and Cx43 proteins revealed the presence of Cx30.3 ... an early stage of differentiation Among 20 Cx isoforms in mouse genome, the gene expressions of 19 isoforms were detected in the pluripotent state of EB3 cells (Fig. 1a) and 15 of them showed ... overexpression on the shape of cells and colonies (i) EB3 cells in LIF(− ) for 6 d, (ii) Cx30.3 overexpressing EB3 cells in LIF(− ) for 6 d. Trang 6Figure 4 Localization of Cx30.3 protein and/or Cx43 protein...

Ngày tải lên: 19/11/2022, 11:41

... sequences, and mixed patterns of enrichment in the 3′ UTR Degree and pattern of methylation distinguish transcripts modified in both embryonic stem cells and brain from those methylated in either one of ... unbiased global analysis of m5C in total and nuclear poly(A) RNA of mouse embryonic stem cells and murine brain We show that there are intriguing differences in these samples and cell compartments ... sequencing (RNA BS-seq) in embryonic stem cells (ESCs) and the brain in total and nuclear poly(A) RNA and compared its prevalence and distribu-tion in both cell/tissue types and cellular compartments...

Ngày tải lên: 24/11/2022, 17:43

... Trang 1DIFFERENTIATION AND DERIVATION OF LINEAGE-COMMITTED CHONDROPROGENITORS AND CHONDROGENIC CELLS FROM HUMAN EMBRYONIC STEM CELLS FOR CARTILAGE TISSUE ENGINEERING AND REGENERATION TOH WEI ... Associate Professor Cao Tong, Vice-Dean (Research), Department of Oral & Maxillofacial Surgery, Faculty of Dentistry, National University of Singapore, and Professor Lee Eng Hin, Director of Graduate ... 84.1 PHASE I: MODEL SYSTEM……… 65 4.1.1 Pluripotency of human embryonic stem cells……… 65 4.1.2 Effects of culture conditions in modulation of chondrogenesis…….66 4.1.3 Effects of culture conditions...

Ngày tải lên: 11/09/2015, 09:57

... haematopoietic stem cells that give Trang 21rise to blood cells (Moore and Lemischka, 2006; Petersen and Terada, 2001) (Figure 1.2) Stem cells, particularly embryonic stem cells (ESCs), offer enormous ... differentiation of mESCs reduces Oct4 and Sox2 levels and binding, and abolishes Stat3 and Smad1 binding 7.10 Knockdown of Oct4 and Sox2 in mESCs differentiates the cells, significantly reduces Oct4 and ... binding of Oct4, Sox2, Stat3 and Smad1 in mouse embryonic stem cells as annotated in NCBI Trang 14LIST OF FIGURES Figure 1.1 A schematic view of mouse pre-implantation development 1.2 Mouse embryonic...

Ngày tải lên: 11/09/2015, 16:03

... pluripotent embryonic stem cells (ESCs) and pluripotent cells of the early embryo, thus gaining their characteristic developmental potential and other characteristics diagnostic of pluripotent cells ... SCREEN OF NUCLEAR RECEPTORS FOR THE ENHANCED AND ALTERNATIVE GENERATION OF INDUCED PLURIPOTENT STEM CELLS DOMINIC HENG JIAN CHIEN (B.Sc (Hons), NTU) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF ... expressed in epiblast stem cells (EpiSCs) and primordial germ cells (PGCs) 12 The importance of this transcription factor is evidenced by the fact that the ablation of Oct4 results in embryonic lethality...

Ngày tải lên: 09/09/2015, 18:56

... embryonic stem cell; hESC, human embryonic stem cell; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of lysine 27 of ... pluripotent embryonic stem cells and differentiation Nat Rev Mol Cell Biol 2006, 7:540-546 51 Park SH, Kook MC, Kim EY, Park S, Lim JH: Ultrastructure of human embryonic stem cells and spontaneous and ... embryonic stem cell; H3K4me1, methylation of lysine of histone H3; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of...

Ngày tải lên: 11/08/2014, 12:21

... Human Embryonic Stem Cells (hESCs) 38 4.2.1 Culture of Human Embryonic Stem Cells on MEF feeders 38 4.2.2 Feeder- free Culture of Human Embryonic Stem Cells 39 4.2.3 Coating of Matrigel ™ ... development of cardiac cells from IPSCs 14 Literature Review 3.1 Human Embryonic Stem Cells (hESCs) Human Embryonic Stem Cells are pluripotent cells derived from the inner cell mass of the developing ... Aims and objectives 14 Literature Review 15 3.1 Human Embryonic Stem Cells (hESCs) 15 3.2 Porcine Embryonic Stem Cells 16 3.3 Generation of Induced Pluripotent Stem Cells...

Ngày tải lên: 30/09/2015, 10:13

... experience, skill and knowledge in the derivation, culture and expansion of human embryonic stem cells, and more recently, of human induced pluripotent stem cells The exploration and realization of the ... translational research in the field of embryonic and induced pluripotent stem cells It commences with practical aspects of the derivation and growth of human embryonic stem cells from inner cell mass ... pluripotent stem cells The culmination of two events has enabled the production of human embryonic stem cells: the birth of the first IVF test-tube baby in 1978 (Steptoe and Edwards 1978) and the...

Ngày tải lên: 22/03/2014, 09:20

... m l of α-synuclein protein in the form of Lewy bodies and Lewy neurites, and selective loss of dopamin­ rgic (DA) e neurons in the substantia nigra of the mid­ rain [68,69] b The majority of PD ... study of live cell and early aspects of disease pathogenesis For AD, there are many attractive targets for this type of analysis, including the toxicity, clearance and localization of Aβ and other ... GQ, Porteus MH, Joung JK, Cheng L: Gene targeting of a disease-related gene in human induced pluripotent stem and embryonic stem cells Cell Stem Cell 2009, 5:97-110 41 Hockemeyer D, Soldner F,...

Ngày tải lên: 11/08/2014, 12:21

... differentiation of mesenchymal progenitor cells and to up regulate the levels of type II collagen and aggrecan in chondrocytes and chondroprogenitor cells [1,7-11] During development of the limbs, ... of primary MSCs and to investigate whether levels and extent of hypertrophy in vitro is influenced by the choice of transgene Materials and methods Construction and preparation of recombinant ... cartilage, and CS4, one of the monomers of the polysaccharide portion of proteoglycan, showed significantly enhanced production of these cartilage matrix proteins at days 10 and 21 of culture...

Ngày tải lên: 09/08/2014, 14:22

... Feinberg AP: Differential methylation of tissue- and cancer-specific CpG island shores distinguishes human induced pluripotent stem cells, embryonic stem cells and fibroblasts Nat Genet 2009, 41:1350-1353 ... human embryonic stem cells Cell 2005, 122:947-956 Cole MF, Johnstone SE, Newman JJ, Kagey MH, Young RA: Tcf3 is an integral component of the core regulatory circuitry of embryonic stem cells ... References Young RA: Control of the embryonic stem cell state Cell 2011, 144:940-954 Ben-David U, Benvenisty N: The tumorigenicity of human embryonic and induced pluripotent stem cells Nat Rev Cancer...

Ngày tải lên: 11/08/2014, 12:21

... Page of 11 Figure Mesenchymal stem cells (MSCs) inhibit the proliferation of CD4 + T cells in vitro by induction of nitric oxide and prostaglandin E2 (PGE2) (a) CD4+ T cells, in the presence of ... http://arthritis-research.com/content/12/1/R31 Page of 11 Figure Phenotype and differentiation potential of mesenchymal stem cells (MSCs) Bone marrow cells of DBA/1 wild-type and interferon-gamma receptor knockout ... Materials and methods Isolation and expansion of mesenchymal stem cells DBA/1 mice were bred in the Animal Centre of the University of Leuven Bone marrow from 4- to 6-week-old DBA/1 and DBA/1...

Ngày tải lên: 12/08/2014, 11:23

... erythroid differentiation of murine embryonic stem cells PCR analysis of DNase I treated and reverse-transcribed total RNA extracted from differentiating embryonic stem cells at the indicated time ... ES cells and in human hematopoietic progenitor cells Discussion B Fig Transcription of LCR hypersensitive site in undifferentiated murine embryonic stem cells and in human CD 133+ bone marrow cells ... the developmental progression of cell samples and in all cases except that of the Rex-1 gene, primer sets span introns Day cells are composed of ES cells and MEF cells grown in ES media containing...

Ngày tải lên: 07/03/2014, 12:20

... either EB or adult cells (Figure 6, panel A) The difference in the expression of miR-200c, miR-302b, and miR-367 between hES cells and EB, and between hES cells and adult cells was significant ... in adult cells (Figure 6, panel C) The differences in expression of these miRNA expression between adult cells and hES cells and between adult cells and EB were significant (P < 0.05) Of note, ... expression pattern of undifferentiated and differentiated human embryonic stem cells Stem Cells Dev 2007, 16(6):1003-1016 Page 15 of 17 (page number not for citation purposes) Journal of Translational...

Ngày tải lên: 18/06/2014, 15:20

... because of the limited amount of sample from patients and normal donors On the other hand, in vitro culture is capable of sustaining a large number of cells, which can confer specific advantages and ... Thailand and two normal donors All patients were children 3-12 years-old and had symptoms of severe anemia and hepatosplenomegaly Hemoglobin analysis showed no expression of the b-hemoglobin band ... The isolated HSCs/CD34+ cells were harvested for phosphoproteomic analysis and cultivation of erythroid cells The cultivation of erythroid cells derived from HSCs/CD34+ cells was performed according...

Ngày tải lên: 18/06/2014, 19:20