electrospray ionization and ion trap mass spectrometry it ms

... Retention time, monitored SIM ion and regression equation for 16 PAHs by GC-MS t R (min) SIM ion (m/z) standard (IS) Recovery, reproducibility and the analysis limits The recovery and reproducibility ... system with a pulsed splitless mode The MS spectrum analyzed the PAHs in two forms: TIC (total ion current) and SIM (selective ion monitoring) in positive ion mode (Table 1) RESULTS AND DISCUSSION ... with a linear concentration from 10 to 100ppb and coefficients of determination r2 > 0.99 In addition, the limit of detection for PAHs concentrations (0.3-0.8ppb) allowed the quantification

Ngày tải lên: 20/12/2019, 16:39

... Retention time, monitored SIM ion and regression equation for 16 PAHs by GC-MS t R (min) SIM ion (m/z) standard (IS) Recovery, reproducibility and the analysis limits The recovery and reproducibility ... system with a pulsed splitless mode The MS spectrum analyzed the PAHs in two forms: TIC (total ion current) and SIM (selective ion monitoring) in positive ion mode (Table 1) RESULTS AND DISCUSSION ... with a linear concentration from 10 to 100ppb and coefficients of determination r2 > 0.99 In addition, the limit of detection for PAHs concentrations (0.3-0.8ppb) allowed the quantification

Ngày tải lên: 14/01/2020, 17:37

... system with a pulsed splitless mode The MS spectrum analyzed the PAHs in two forms: TIC (total ion current) and SIM (selective ion monitoring) in positive ion mode (Table 1) RESULTS AND DISCUSSION ... (Germany) and 4-digit Ohaus balance (USA) were used for sample preparation All sample preparation equipment (e.g centrifuge tubes) were washed with soap and water and rinsed with acetone and n-hexane ... Nguyen Van Phuc Determination of polycyclic aromatic hydrocarbons Table Retention time, monitored SIM ion and regression equation for 16 PAHs by GC-MS Naphthalene 4.51 SIM ion (m/z) 128 Acenaphthylene

Ngày tải lên: 02/03/2020, 16:02

... Retention time, monitored SIM ion and regression equation for 16 PAHs by GC-MS t R (min) SIM ion (m/z) standard (IS) Recovery, reproducibility and the analysis limits The recovery and reproducibility ... system with a pulsed splitless mode The MS spectrum analyzed the PAHs in two forms: TIC (total ion current) and SIM (selective ion monitoring) in positive ion mode (Table 1) RESULTS AND DISCUSSION ... with a linear concentration from 10 to 100ppb and coefficients of determination r2 > 0.99 In addition, the limit of detection for PAHs concentrations (0.3-0.8ppb) allowed the quantification

Ngày tải lên: 01/05/2021, 11:19

... Ionization Desorption and Field Ionization Techniques? 2 Field Desorption and Field Ionization The Principles of Field Desorption and Field Ionization Practical Operation of Field Desorption and ... tmospheric Pressure Ionization Techniques - Electrospray Ionization and Atmospheric Pressure Chemical Ionization Atmospheric Pressure Ionization Techniques? Atmospheric pressure ionization (API) encompasses ... Ionization Methods in Organic Mass Spectrometry Chapter 2 Atmospheric Pressure Ionization Techniques - Electrospray Ionization and Atmospheric Pressure Chemical Ionization Atmospheric Pressure Ionization

Ngày tải lên: 18/05/2022, 09:28

... obtained by 16S-ARDRA and MALDI-TOF MS Isolate Source Identification by 16S rDNA sequence Identity value GenBank accession no Identification by 16S-ARDRA (% similarity between wild and reference strains, ... correct genus identification but only presumptive species identification The 16S-ARDRA protocol was based on digestion of 16S rDNA with the restriction enzymes MseI, HinfI, MboI and AluI This technique ... Access 16S-ARDRA and MALDI-TOF mass spectrometry as tools for identification of Lactobacillus bacteria isolated from poultry Marta Dec* , Andrzej Puchalski, Renata Urban-Chmiel and Andrzej Wernicki

Ngày tải lên: 19/11/2022, 11:35

... (=trueness and precision, i.e repeatability and intermediate precision), limit of detection (LOD), limit of quantification (LOQ), assay range, sensitivity and robustness Other specific criteria can ... identification cannot be performed with the usual algorithms applied to the recognition of full spectra Deconvolution algorithms are useful for identification purposes, but their quantitative reliability ... advisable to apply the IP calculation to the ions extracted from full spectra The auto-ionization and adduct formation trend of old hyperbolic ion traps has been observed, and such a risk should be carefully

Ngày tải lên: 04/12/2022, 10:36

... mg of vitamin B1, 0.06 mg of vitamin B2, 0.5 mg of vitamin PP, and a significant 30 mg of vitamin C. Table 2.1: Nutritional value per 100g radish Encyclopedia of Food Sciences and Nutrition (Second ... grams of protein, 3.7 grams of carbohydrates, and 1.5 grams of fiber Additionally, it contains 40 mg of calcium, 41 mg of phosphorus, and 1.1 mg of iron Radishes also offer small amounts of vitamins, ... (LC-MS /MS)”.Research’s objectiveThe objective for this study is evaluate the sensitive and accurate method based on: Linearity, Repeatability, Recovery, Reproducibility, Limit of detection (LOD),

Ngày tải lên: 16/12/2021, 17:08

... sensitive and accurate method based on: Linearity, Repeatability, Recovery, Reproducibility, Limit of detection (LOD), Limit of quantification (LOQ) - Why choose Liquid Chromatography tandem mass ... 0.06 mg of vitamin B2, 0.5 mg of vitamin PP, and a notable 30 mg of vitamin C. Table 2.1: Nutritional value per 100g radish Encyclopedia of Food Sciences and Nutrition (Second Edition), 2003 ... leading to nausea and severe diarrhea Additionally, neurological symptoms may include drowsiness, coma, convulsions, and muscle paralysis These conditions can result in hypotension and respiratory

Ngày tải lên: 02/04/2022, 16:30

... phosphorus, and 1.1 mg of iron Additionally, radish contains vital vitamins such as 0.06 mg of vitamin B1, 0.06 mg of vitamin B2, 0.5 mg of vitamin PP, and 30 mg of vitamin C, making it a healthy addition ... Chemical composition and nutritional value Radish is a nutrient-rich vegetable that provides 1.4 grams of protein, 3.7 grams of carbohydrates, and 1.5 grams of dietary fiber per 100 grams It is a good ... tandem Mass Spectrometry (LC-MS /MS)”.Research’s objectiveThe objective for this study is evaluate the sensitive and accurate method based on: Linearity, Repeatability, Recovery, Reproducibility,

Ngày tải lên: 15/02/2023, 08:52

... the surface ionization proba-bility is independent of the angle and energy of the particles emitted On metals, the ionization probability is usually de-pendent on the outgoing velocity.53–57Assuming ... validation concerns the whole dis-tribution of emitted ions in terms of energy and angle It con-firms that the NIEADF for HOPG is close to the neutral distribution function given by SRIM It implies ... the American Institute of Physics Trang 2Negative-ion surface production in hydrogen plasmas: Determinationof the negative-ion energy and angle distribution function using mass spectrometry J P

Ngày tải lên: 04/12/2022, 15:31

... Both MS and MS/MS spectra were acquired in the negative-ion mode with an acquisition rate of 1 s per spectrum over a mass range of m/z 300–2000 (for MS) and m/z 100–2000 (for MS/MS) The ion source ... Conceptualization, Project admin-istration Ming Li: Funding acquisition Fei Ding: Methodology Wen-gang Chai: Funding acquisition, Writing - review & editing Jingyu Yan: Funding acquisition, Writing ... negative-ion ESI-MS for detection and composition analysis of the acidic oligosaccharides as the native reducing sugars and ESI-CID-MS/MS for subsequent sequencing For the low quantity of milk

Ngày tải lên: 01/01/2023, 12:10

... approach with no further separa-tion was evaluated For the tandem mass spectrometry, three different MS instruments were utilized: 1.) a matrix assisted laser desorption ionization tandem mass spec-trometer ... with no further separation The samples were prepared for mass spectrometry analysis by digestion with trypsin Tryptic digests were analyzed by using either a matrix assisted laser desorption ionization ... fractionated the virion into soluble (membrane) and insoluble (core) fractions via treatment with detergent and centrifugation Further fractionation was achieved using two procedures: SDS-PAGE and

Ngày tải lên: 19/06/2014, 08:20

... pretreated with NPDS, and after digestion incubated with propionyl-CoA The identity of domains present in each peak is indicated, together with their inferred acylation status Separation conditions ... studies In our work, ion trap mass spectrometry was used Compared with the Fourier transform -ion cyclotron resonance (FT-ICR) mass spectrometer [16,17], the ion trap is less expensive and more widely ... conclusion, our strategy for limited proteolysis in combination with on-line liquid chromatography ion trap mass spectrometry studies on the multifunctional proteins, DEBS1-TE, DEBS3 and DKS...

Ngày tải lên: 16/03/2014, 18:20

... fragments, fractionate the complex mixture and use high-resolution electrospray ionization Fourier-Transform mass spectrometry Changing patterns of covalent active-site occupancy (ESI ⁄ FTMS) to pick ... for mass deconvolution The detailed conditions for limited proteolysis and LC ⁄ MS analysis have been described previously [37] Substrate specificity for the chain-extender unit A mm concentration ... [12,42] and in vitro [43] 7064 Evidence for coordination of condensation and ketoreduction In previous work [44] the operation of a single extension module from DEBS was studied in vitro by mixing...

Ngày tải lên: 16/03/2014, 03:20

... 100% acetonitrile The solutions were combined and concentrated in a SpeedVac iTRAQ labeling and LC-ESI MS/ MS analysis To label peptides with the iTRAQ reagent (Applied Biosystems, Foster City, CA, ... digestion so that it would not interfere with the LCMS ⁄ MS analysis In addition, we also utilized a recently developed and widely used multiplexed quantitation strategy based on iTRAQ isobaric ... utilized a technology combining gel-assisted digestion, isobaric tags with related and absolute quantitation (iTRAQ) labeling and LC -MS ⁄ MS for quantitative analysis of the membrane proteome of colorectal...

Ngày tải lên: 16/02/2014, 15:20

... ionization' methods such as field desorption and electrospray ionization tend to produce mass spectra with little or no fragment -ion content Gas-Phase Ionization These methods rely upon ionizing ... require either desorption or desolvation methods if they are to be analyzed by mass spectrometry Although ionization and desorption/desolvation are usually separate processes, the term "ionization ... ionization and desorption (or desolvation) methods The choice of ionization method depends on the nature of the sample and the type of information required from the analysis So-called 'soft ionization' ...

Ngày tải lên: 22/03/2014, 14:20

... Determination with Quadrupole MS 6.5.1 Impurity Identification with LC -MS 6.5.2 Impurity Identification with GC -MS 6.5.3 Pros and Cons of Determination of Elemental Decomposition (DEC) with Quadrupole MS ... Compositions by Simple Screening HPLC -MS and Infusion Orbitrap MS 4.9 Determining Substructures: Orbitrap Tandem Mass Spectrometry (MSn) 4.10 Multianalyzer (Hybridized) System: The Linear Ion Trap/ Orbitrap ... electrospray- assisted desorption /ionization electrosonic spray ionization electron transfer dissociation enzyme unit fast-atom bombardment field flow fractionation field-induced droplet ionization...

Ngày tải lên: 28/03/2014, 10:20

... (metabolite candidates) with comparably few dimensions (conditions) In contrast, sample-based clustering implies only few intensity vectors according to the number of conditions and repetitions ... replicas for each condition The identification of metabolites was verified by exact mass measurement and coelution with authentic standards Data processing The raw mass spectrometry data of all ... depends on reproducibility and biological interpretability Without prior selection, large amounts of non-informative markers with little intensity variation across different conditions would dominate...

Ngày tải lên: 12/08/2014, 17:20