electroporation based methods for analysis of zebrafish brain development

... excess of 0.2 m phosphate buffer pH 7.5 Synthesis of the fluorescent Cbl analogue CBC-244 Activation of the 5¢ hydroxyl group in the a-ribofuranoside moiety of CNCbl was performed with help of 1,1¢-dicarbo-nyl-di-(1,2,4-triazole) ... retention of Cbl Additionally, a His residue substituted for water of H2OCbl, which added to protection of the ligand against reduction and coordination of other com-pounds The structure of TC–Cbl ... present article were performed with 5¢ form, and below we will refer to 5¢ CBC-244 as CBC Spectral properties of CBC The coefficient of molar absorbance for rhodamine moi-ety of CBC was estimated...

Ngày tải lên: 19/02/2014, 05:20

... to the different isoforms The relative strength of the segments corresponds to the abundances of the two isoforms (Figure S9 in Additional file 1) Examining levels or changes of particular genes ... the webbased interface offers data analysis, visualization and browsing tools that realize the biological potential of this new technology Methods Calculating P-values for significance of changes ... platform are colored red (exon array) or green (RNA-Seq) and those different on both platforms are colored blue P-value cutoffs are 0.01 for exon array and 10-4 for RNA-Seq, and fold-change cutoffs...

Ngày tải lên: 09/08/2014, 23:20

... Trang 1СТАНДАРТНЫЕ МЕТОДЫ АНАЛИЗА КОНСИСТЕНТНОЙ СМАЗКИ STANDARD TEST METHODS FOR ANALYSIS OF LUBRICATING GREASE Trang 219428, USA ASTM International не утверждает и не подтверждает ... translation is executed by FBU “CIC “Interstandard” of Federal Agency on Technical Regulating and Metrology under the license of American Society for Testing and Materials (ASTM International) 100 ... version published with a sign of ASTM International copyright can be considered as the original version Reproduction of the specified translations by any Party, except for ASTM International or FBU...

Ngày tải lên: 10/03/2024, 04:30

... been approved for use by agencies of the U.S Department of Defense. 1 Scope 1.1 These test methods for analysis cover greases of the conventional type, which consist essentially of petroleum oil ... number of fatty acids, B = millilitres of KOH solution required for titration of the solution, N = normality of KOH solution, and X = grams of fatty acid titrated 15.1.2 Molecular Weight of Fatty ... mean molecular weight of the soap, C = percentage of fatty acids from soap, M f = mean molecular weight of fatty acids from soap, and E = number of moles of fatty acid per mole of soap 15.1.4 Soap...

Ngày tải lên: 10/03/2024, 04:30

... Research Article Analysis of Filter-Bank-Based Methods for Fast Serial Acquisition of BOC-Modulated Signals Elena Simona Lohan Institute of Communications Engineering, Tampere University of Technology, ... whose num- ber of degrees of freedom depends on the method and the number of filters used. Next section presents the parameters of the distribution of Z for each of the analyzed methods. ElenaSimonaLohan ... acquisition time-bin steps of 0.5 chips are used for BPSK modulation (such as for C/A code of GPS), where the width of the main lobe is 2 chips, and steps of 0.1– 0.2 chips are used for SinBOC(1,1) modulation,...

Ngày tải lên: 22/06/2014, 19:20

... for uniform regions and regions with smooth transitions Region based detectors regard local blobs of uniform brightness as the most salient aspects of an image and are therefore more suited for ... section we discuss global appearance-based methods for object recog-nition In fact, the discussion is reduced to subspace methods The main idea for all of these methods is to project the original ... summarized more formally in Algorithm 3.For our application we use this implementation of PCA for two reasons:(a) the computation of SVD is numerically often more stable than the com-putation of the EVD...

Ngày tải lên: 24/04/2014, 13:47

... Figure 2 Analysis of recombinant Arabidopsis HECT-type E3 ligases (UPL7 and UPL5) A, Production of FLAG-tagged recombinant UPL proteins was detected by immunoblot analysis For analysis, 5 μl of crude ... result of polyubiquiti-nation analysis of the E2s obtained from luminescent-based detection method was verified by immunoblot analysis (Fig 1) Our analysis also produced recombinant protein of HECT-type ... and used for sev-eral functional analyses For screening, conventional detection methods such as immunoblot analysis or radio-isotope-labeled proteins are not suitable for the detection of a large...

Ngày tải lên: 12/08/2014, 03:20

... DEVELOPMENT, EVALUATION AND OPTIMIZATION OF IMAGE BASED METHODS FOR MONITORING CRYSTALLIZATION PROCESSES ZHOU YING NATIONAL UNIVERSITY OF SINGAPORE 2011 DEVELOPMENT, EVALUATION ... Validation of Predicted Optimal Parameters for Uniform Design - 139 Table 7.4 SSO Performance with Different Initial Guess and Step Size (1st set of images) 142 Table 7.5 Performance of 1st ... L. & Mrak, J., (1997), Quantitative FT-Raman analysis of two crystal forms of a pharmaceutical compound. Journal of Pharmaceutical and Biomedical Analysis, 15, 687-696. Larsen, P.A., Patience,...

Ngày tải lên: 10/09/2015, 15:51

... DATA-BASED METHODS FOR MODELING, CONTROL AND MONITORING OF CHEMICAL PROCESSES CHENG CHENG NATIONAL UNIVERSITY OF SINGAPORE 2006 DATA-BASED METHODS FOR MODELING, CONTROL AND MONITORING OF CHEMICAL ... information poor” is a common problem for most chemical processes Therefore, how to extract useful information from data for the purposes of process modeling, control, and monitoring is one of ... monitoring using model-based PCA Proceedings of the 5th IFAC Symposium of Dynamics and Control of Process Systems, Corfu, Greek, pp 86-81 Wigren, T (1994) Convergence analysis of recursive identification...

Ngày tải lên: 30/09/2015, 05:58

... isdifferent in the aspect of lack of collocation points The DSG Trang 2method is therefore independent of the order and form of theelement On an other front of development of finite element nology, Liu ... andeffectiveness of the present method The layout of the paper is as follows Next sectiondescribes the weak form of governing equations and the for-mulation of 3-node plate element In Sect.3, a formulation of ... alternative form of nodally integrated techniques in finite element tions [61–68] The crucial idea of these methods is toformulate a nodal deformation gradient via a weightedaverage of the surrounding...

Ngày tải lên: 16/12/2017, 05:10

... Therefore, although these methods yield acceptable solu-tions for design of structure and save lots of computational efforts, they have been gradually being replaced by advanced analysis methods ... advanced analysis Fig 4 shows the his-togram of ultimate load-factor of the truss with the number of samples of 50,000 and Keras are employed, while PAAP program [4] is used for performing advanced analysis ... the input of the unit of the layer; is the weight at the connection between the unit of the layer and the unit of the layer; is the number of units of the layer In each layer, to transform the...

Ngày tải lên: 12/01/2020, 02:44

... spectrometry imaging for more comprehensive analysis of the material complexities of the brain 3.1 The ideal chemical microscope for biological materials Techniques for in situ metal imaging analysis depend ... particular role of X-ray methods for elemental imaging analysis of accumulated metal species and metal-containing compounds in biological materials, in the context of post-mortem brain tissue X-rays ... recently as an essential element for tissue development and architecture [20] The main role of iodine is as a constituent of thyroid hormones required for brain development Sulfur and selenium...

Ngày tải lên: 24/11/2022, 17:38

... 1MINISTRY OF EDUCATION AND TRAINING VIETNAM ACADEMY OF SCIENCE AND TECHNOLOGY GRADUATE UNIVERSITY OF SCIENCE AND TECHNOLOGY - TRẦN VĂN PHÚC STUDY ON APPLICATION OF THE NUCLEAR METHODS FOR ANALYSIS ... tasks of the research The first goal of the thesis is to grasp the principles, experiments and applications of the Rutherford Backscattering Spectrometry (RBS) method in the analysis of materials, ... irradiation in the energy range of 100-250 keV using one of the ion beam analysis methods - Rutherford Backscattering Spectrometry (RBS) ii) Investigation dependence of ion-induced mixing at TiO2/SiO2...

Ngày tải lên: 27/02/2024, 07:50

... commissure Chapter – Electroporation- based methods for analysis of zebrafish brain development 3.1 Overview In order to investigate mechanisms of habenular commissure development, we required ... Formation of the habenular commissure 21 ii 2.9 CONCLUSIONS 22 2.10 EXPERIMENTAL PROCEDURES 22 CHAPTER – ELECTROPORATION- BASED METHODS FOR ANALYSIS OF ZEBRAFISH ... population of neurons This chapter presents a suite of techniques developed or optimized for the study of neural development in zebrafish Electroporation is a technique for the introduction of nucleic...

Ngày tải lên: 14/09/2015, 10:34

... has been established for Arabidopsis and rice [2–4] However, although plants encode for a large number of BTB proteins, a functional role has only been assigned for a few of them, including ETO1 ... interactors for the MATH domain, but would also provide information on to what extent the two different MATH domains of BPM1 and BPM3 target the same group of proteins (see Table S1A,B for identity ... expression strength ⁄ intensity: For BPM1 and BPM3 twofold, and for BPM4 threefold, the amount of RT-PCR product was loaded (compared with actin2 control reaction) (B) RT-PCR analysis showing BPM1, BPM2,...

Ngày tải lên: 18/02/2014, 06:20

... Hsp27 Hdj-2 is an isoform of Hdj-1 that has been previously shown not to decrease htt inclusion body formation in COS-7 cells [39] Immunoblot analysis of the intracellular level of Hsps revealed ... the percentage of cells presenting inclusions as well as the distribution of the size of the inclusions were dependent on when the analysis was performed after transfection Therefore, all the ... analyzed the activity of the Hsp70 ⁄ Hdj-1 reconformation machine by cotransfecting COS-7 cells with vectors encoding Hsp70 and the nonactive isoform of Hsp40, Hdj-2 In the presence of Hsp70 ⁄ Hdj-2,...

Ngày tải lên: 19/02/2014, 06:20

... identifying new classes of antibiotics displaying diverse mode(s) of action: antimicrobial peptides are currently considered to be some of the most promising candidates for the development of novel anti-infective ... (µM) % of lactamase release B 100 80 60 40 20 10 32 15 20 Time (min) Fig Permeation of E coli OM by Esc(1–18) (A) Effects of different concentrations of Esc(1–18) on permeation of the OM of E coli ... Permeation of the bacterial IM To assess the ability of Esc(1–18) to alter the permeability of the IM of E coli, · 106 cells were mixed with lm SYTOX Green in NaCl ⁄ Pi for in the dark After addition of...

Ngày tải lên: 16/03/2014, 00:20

... 150 V for  h For autoradiography, wet gels were exposed for h to a Kodak X-Omat film at )80 °C To ensure the complexes were formed by the association of proteins and nucleic acids, lg of each ... not part of any of the three LaGT complexes The values estimated for the protein masses included the mass of the Tel6 oligonucleotide ( 5.6 kDa) The binding specificity of LaGT protein-forming ... bands of  35 kDa and  52 kDa and the UV-irradiated complex of  18–20 kDa were in-gel digested with trypsin and subjected to MALDITOF MS and ESI-MS/MS analysis The MALDI-TOF spectra obtained for...

Ngày tải lên: 23/03/2014, 13:20

... 25S formation in Dnop53 ⁄ A-NOP53, compared with the much less affected formation of mature 18S rRNA (Fig 4B) Analysis of pre-rRNA and rRNA steady-state levels by means of northern blot was performed ... rRNA Analysis of rRNA processing showed that depletion of Nop53p leads to an accumulation of the 27S and 7S pre-rRNAs, confirming a role for Nop53p on late steps of processing Accumulation of unprocessed ... was performed after incubation of pmol of in vitro transcribed, uniformly labeled 5.8S rRNA with increasing amounts of His–Nop53p or bovine serum albumin (NEB), in the absence or presence of cold...

Ngày tải lên: 20/02/2014, 01:20

... Ssa1p binds a hexameric form of Ure2p in the presence of ATP, whereas the form that is bound in the presence of ADP is different, and probably dimeric [6] We therefore performed Ure2p and Ssa1p ... assembly of Ure2p into fibrils [33] Thus, the binding of Ssa1p in the vicinity of this stretch interferes with Ure2p assembly into fibrils either because of a change in the conformation of this ... with 5% formic acid at 37 °C for 15 The extracted peptides were vacuum dried, dissolved in 1% formic acid and stored at )20 °C until MS analysis High mass MALDI-TOF MS High-mass MALDI-TOF mass...

Ngày tải lên: 15/03/2014, 00:20