dna sequencing using fluorescence detection

... diagnostic methods for the detection of intracellular viral DNAs in paraffin sections J Vet Med Sci 2003, 64, 231-235 73 Kim O, Chae C In situ hybridization for the detection and localization ... cells infected with 103 TCID50 using overnight hybridization protocol However, biotin-labeled probe was able to detect CHV in MDCK cells infected with 104 TCID50 By using digoxigenin-labeled probe, ... than those of biotin-labeled probe in the hybridization assay for the detection of CHV These results suggest that ISH assay using digoxigenin-labeled probe, which was optimized in this study, may...

Ngày tải lên: 07/08/2014, 17:22

... sequenced using the 100bp paired-end module, and sequencing was conducted using a newer version of Illumina’s sequencing chemistry These 24 libraries occupied approximately 5% of the total sequencing ... Validation by Sanger sequencing Sanger sequencing of positive pools for variant validation was conducted using the BigDye Terminator v3.1 Cycle Sequencing Kit on an ABI3100 automatic DNA analyzer (Applied ... Keywords Next-generation Sequencing, Sample-Pooling, Multiplexed Sequencing, Rare Variant Detection, Variant Filtering Algorithms, SRFIM, SERVIC4E Background Next-generation sequencing and computational...

Ngày tải lên: 09/08/2014, 23:20

... CONCLUSION 84 CHAPTER 7: REFERENCES 86 CHAPTER 8: APPENDIX 96 v SUMMARY Detection of low grade mosaicism in Turner syndrome using fluorescence in-situ hybridization (FISH) Turner syndrome is a common ... New York : Elsevier, 1995 21 2.5: Fluorescence in-situ hybridization: Fluorescence in-situ hybridization (FISH) is a technology utilizing fluorescently labeled DNA probes (with a fluorescent dye ... frequently used fluorochromes for the detection of the FISH signals are FITC (fluorescein isothiocyanate) that emits a green fluorescence and dyes with orange or red fluorescence, such as Cy3, or TexasRed...

Ngày tải lên: 04/10/2015, 15:45

... the researchers in the field of DNA sequencing An overview of DNA sequencing technologies right from the Sanger’s method to the next generation high throughput DNA sequencing techniques including ... signature sequencing, polony sequencing, pyrosequencing, Illumina Sequencing, SOLiD sequencing etc has been presented in chapter Chapter reviews how Hot Start-7-deaza-dGTP improves Sanger’s dideoxy sequencing ... rapid speed of sequencing attained with modern DNA sequencing technology has been instrumental in the sequencing of the human genome, in the human genome project Fig DNA Sequence Trace DNA can be...

Ngày tải lên: 08/03/2014, 19:20

Ngày tải lên: 21/03/2014, 01:20

... the error made using set k z= σd and σd = erri (1−erri ) ins + errj (1−errj ) ins Conclusions In this paper, we present a novel way to semantically represent negation using focus detection Implicit ... revealing implicit positive meaning The main contributions are: (1) interpretation of negation using focus detection; (2) focus of negation annotation over all PropBank negated sentences1 ; (3) feature ... and ¨ u Ozg¨ r and Radev (2009) propose scope detectors using the BioScope corpus Councill et al (2010) present a supervised scope detector using their own annotation Some NLP applications deal...

Ngày tải lên: 30/03/2014, 21:20

... identify suspects? How reliable is DNA evidence? 18 5.1 Using the NDNAD to identify suspects 18 5.2 DNA profiling is not foolproof .19 5.3 Using DNA evidence in court ... What is the National DNA Database? 11 3.1 Using DNA to identify individuals 11 3.2 How the police use DNA 12 3.3 Concerns about police use of DNA our right to privacy ... future of DNA profiling 30 7.1 Predicted changes to DNA profiling .30 7.2 Using DNA profiles to predict the characteristics of suspects .31 The future of the NDNAD ...

Ngày tải lên: 30/03/2014, 22:20

... single-stranded DNA phage in DNA sequencing, in Nucleic Acids Sequencing A Practical Approach (Howe, C J and Ward, E S., eds.), IRL Press, Oxford, pp l-36 Anderson, S (1981) Shotgun DNA sequencing ... Ml3 DNA to mmimaze single-stranded Ml3 DNA contammation Even so, sometimes the mmiprepared RF Ml3 DNA is still contaminated with singlestranded Ml3 DNA, which may confuse the pattern of DNA fragments ... the adventof cycle sequencing( Chapter 26) has vastly increased the range of templates that can be used Sequencing Strategies A lot of sequencing performed is confirmatory sequencing to check...

Ngày tải lên: 11/04/2014, 09:40

... current ABI PRISM DNA sequencing kits use cycle sequencing protocols See Chapter for information on cycle sequencing protocols Figure 1-3 Cycle sequencing Advantages of Cycle o Sequencing o Protocols ... ABI PRISM DNA sequencing kits 1-6 Introduction Applied Biosystems DNA Sequencing Instruments ABI 373 The ABI 373 DNA Sequencer is an automated instrument for analyzing uorescently DNA Sequencer ... troubleshooting sequencing data Refer to the ABI PRISM DNA Sequencing Analysis Software Users Manual for specic information about the Sequencing Analysis software 1-16 Introduction ABI PRISM DNA Sequencing...

Ngày tải lên: 02/06/2014, 09:23

... objectives To understand how DNA is sequenced using the most common technique automated dideoxynucleotide sequencing Content INTRODUCTION GENOMIC DNA DNA PREPARATION SEQUENCING REACTION STRAND ... GENOMIC DNA First, DNA has to be extracted from the cells of the organism being studied The sequencing reaction is then performed on the DNA, and the sequenced DNA strands are sorted by size using ... million bases in length Viral and bacterial DNA sequences, which not contain introns, are typically the shortest genes 16 17 GENOMIC DNA The dideoxy DNA sequencing procedure was invented by Frederic...

Ngày tải lên: 29/06/2014, 13:20

... chickens using different detection methods and to define the molecular characteristics of the isolates using multiplex PCR and RAPD The conventional culture method showed the lowest detection ... had been shown not to amplify products detectable by agarose gel (Sigma, USA) electrophoresis using DNA templates derived from a range of Gram-positive and Gram-negative bacterial species from ... for 90 s The final extension cycle was followed by at 72oC for Amplified DNA fragments were resolved by gel electrophoresis using 1.5% agarose gels in Trisacetate-EDTA (TAE) buffer Gels were stained...

Ngày tải lên: 07/08/2014, 18:21

... directions n using the following equation for each pixel in the image: ∇ (G × g) n = ∇ (G × g) (1) Determination of OSB fiber orientation (4) Find the location of the edges using the ... cycle, For Prod J 17 (1969) 51–57 [9] Tsai D.M., Hou H.T., Su H.J., Boundary-based corner detection using eigenvalues of covariance matrices, Pattern Recognition Lett 20 (1999) 31–40 To access ... technique of image analysis (IA) [6] In this paper, a novel image processing method based on line detection analysis was proposed to automatically detect fiber orientation on OSB surface with the...

Ngày tải lên: 08/08/2014, 00:22

... indirect mechanism of detection by a gamma detection probe, can result in relatively high background counts and can potentially complicate tumor detection efficiency of the gamma detection probe during ... within the gamma detection probe system Two general categories of gamma detection probe systems exist that can be utilized within the operating room environment These include gamma detection probe ... amplified by the detection unit There are advantageous and disadvantageous features that are specific to scintillation-type detection systems and to semiconductor ionization-type detection systems...

Ngày tải lên: 09/08/2014, 04:21

... Maize genome sequencing project Plant Physiol 2001, 127:1572-1578 Lander ES, Linton LM, Birren B, Nusbaum C, Zody MC, Baldwin J, Devon K, Dewar K, Doyle M, FitzHugh W, et al.: Initial sequencing ... A, Jiang N, Tibbitts DC, Wessler SR, Paterson AH: Integration of Cot analysis, DNA cloning, and high-throughput sequencing facilitates genome characterization and gene discovery Genome Res 2002, ... polypeptides will be captured by one or other technique The application of genome filtration for sequencing the maize genome would require the mapping of sequences onto physical or genetic maps,...

Ngày tải lên: 09/08/2014, 20:20

... Table EBV DNA load and antibody titre detection rates in the population samples (Group 5, n = 218) Target Positive n (%) Detectable Range Spearman correlation (p) EBNA-1 DNA load BHRF-1 DNA load ... Targets DNA load EBV EBNA-1 DNA load (copies/ml) 24 (11.0%) 2.0 × 102 - 9.1 × 104 1.00 EBV BHRF-1 DNA load (copies/ml) 47 (21.6%) 2.0 × 102 - 3.3 × 104 0.63 p < 0.001 1.00 Combined EBV targets DNA ... 100% EBV DNA positivity in whole blood, although DNA loads were all below the detection limit of the assay (2.0 × 103 copies/ml) [38] In the population sample the EBV VCA IgG antibody detection...

Ngày tải lên: 12/08/2014, 01:22

... DNA was isolated from 10 independent clones of Escherichia coli by a modified alkaline lysis procedure [12] and sequenced by using quick start cycle DNA sequencing kit (Beckman-Coulter) The DNA ... technically demanding, LiPA and direct DNA sequencing yielded accurate identification of all genotype A and genotype D and mixed genotype A and D strains of HBV The DNA sequencing data reported in this ... designed the study and FH collected the specimens MMA carried out the DNA Extraction, INNO-LiPA genotyping assay, Direct DNA Sequencing, PCR-RFLP studies, sequence alignments and analysis SA participated...

Ngày tải lên: 12/08/2014, 04:20

... IFA) by the National Veterinary Institute, Uppsala, Sweden Total DNA was extracted from whole blood or serum using the QIAGEN QIAamp DNA Blood Mini Kit based on the manufacturer's instructions (QIAgen, ... concentration of the eluted DNA should be 15-60 ng/μl (3-12 μg of DNA eluted in 200 μl of buffer AE) Due to low sensitivity in the PCR reactions modified methods for extraction of DNA were tested and ... appears to be a good source of A phagocytophilum DNA, and has been used extensively in other studies [3,8] In DNA extracts from serum samples less host DNA would be present to interfere with the PCR,...

Ngày tải lên: 12/08/2014, 18:22

Ngày tải lên: 13/08/2014, 14:43